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This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptiona... See more...
Aliases for POLR2B Gene
Aliases for POLR2B Gene
External Ids for POLR2B Gene
- HGNC: 9188
- NCBI Entrez Gene: 5431
- Ensembl: ENSG00000047315
- OMIM®: 180661
- UniProtKB/Swiss-Prot: P30876
Previous GeneCards Identifiers for POLR2B Gene
- GC04P057648
- GC04P057841
- GC04P057760
- GC04P057685
- GC04P057538
- GC04P053798
- GC04P057844
Summaries for POLR2B Gene
-
This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
GeneCards Summary for POLR2B Gene
POLR2B (RNA Polymerase II Subunit B) is a Protein Coding gene. Diseases associated with POLR2B include Fusariosis and Eumycotic Mycetoma. Among its related pathways are Nucleotide excision repair and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include DNA-directed 5'-3' RNA polymerase activity. An important paralog of this gene is POLR3B.
UniProtKB/Swiss-Prot Summary for POLR2B Gene
-
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
Additional gene information for POLR2B Gene
- HGNC (9188)
- NCBI Entrez Gene (5431)
- Ensembl (ENSG00000047315)
- OMIM® (180661)
- UniProtKB/Swiss-Prot (P30876)
- Open Targets Platform(ENSG00000047315)
- Monarch Initiative
- Search for POLR2B at DataMed
- Search for POLR2B at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for POLR2B Gene
Genomics for POLR2B Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for POLR2B Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around POLR2B on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for POLR2B
- Top Transcription factor binding sites by QIAGEN in the POLR2B gene promoter:
-
- C/EBPalpha
- CUTL1
- E47
- FOXF2
- HTF
- ITF-2
- NF-kappaB
- NF-kappaB1
- Tal-1beta
- TBP
Genomic Locations for POLR2B Gene
Latest Assembly
- chr4:56,977,722-57,031,158
- (GRCh38/hg38)
- Size:
- 53,437 bases
- Orientation:
- Plus strand
Previous Assembly
- chr4:57,845,062-57,897,324
- (GRCh37/hg19 by Entrez Gene)
- Size:
- 52,263 bases
- Orientation:
- Plus strand
- chr4:57,843,888-57,897,334
- (GRCh37/hg19 by Ensembl)
- Size:
- 53,447 bases
- Orientation:
- Plus strand
Genomic View for POLR2B Gene
Genes around POLR2B on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 4q12 by HGNC
- 4q12 by Entrez Gene
- 4q12 by Ensembl
POLR2B Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for POLR2B Gene
Proteins for POLR2B Gene
-
Protein details for POLR2B Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P30876-RPB2_HUMAN
- Recommended name:
- DNA-directed RNA polymerase II subunit RPB2
- Protein Accession:
- P30876
- A8K1A8
- Q8IZ61
Protein attributes for POLR2B Gene
- Size:
- 1174 amino acids
- Molecular mass:
- 133897 Da
- Quaternary structure:
-
- Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with WDR82. Interacts with MEN1.
- Miscellaneous:
-
- The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity).
Protein Expression for POLR2B Gene
Selected DME Specific Peptides for POLR2B Gene
- P30876:
-
- AVGLVKNLALMAYISVGS
- FGEMERD
- GVSQVLNR
- AMGKQAMG
- HMVDDKIH
- PQIGDKF
- PILEFLEEW
- EGRSRDGGLR
- IFIGPTY
- THCEIHP
- GPTYYQRL
- TDAGRICRPL
- PSRMTIG
- GDKFASR
- TTRSMEYL
- IGKIPIM
- IPFPDHNQSPR
- LNECPLDPGGYF
- GKLAKPRQLHN
- INGSEKVLIAQE
- GGYFIING
- PMMPNEARLRNLTY
- KLRRQMDIIVSEVSM
- LYYPQKPL
- NPHAIPSRM
- FCETKKAYF
- KLLFQEL
- ATKIFVNG
- PLYVDITK
- CPAETPEG
- GEMERDC
- HNQSPRNTYQ
- GYNQEDS
- DPEMMEMVKPSLDEAFV
- SLDEAFV
- ARLRNLTYSAPLYVD
- STLSHLRR
- LATGNWG
- DGLIAPGVRVSG
- KRLDLAGPLL
- KGLVRQQLDSFD
Post-translational modifications for POLR2B Gene
-
Ubiquitination at Lys95, Lys130, Lys134, Lys146, Lys151, Lys199, Lys214, Lys264, Lys340, Lys421, Lys445, Lys457, Lys578, Lys730, Lys756, Lys821, Lys831, Lys847, Lys869, Lys917, Lys934, Lys942, Lys988, Lys993, Lys1010, Lys1036, Lys1143, and Lys1156
- NX_P30876 (NX_P30876-1)
- Modification sites at PhosphoSitePlus
Other Protein References for POLR2B Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibodies for research
- LifeSpan BioSciences POLR2B / RPB2 Antibody
-
Custom Antibody ServicesOriGene Antibodies for POLR2B
- Novus Biologicals Antibodies for POLR2B
-
Abcam antibodies for POLR2B
- Invitrogen Antibodies for POLR2B (AFLGC-POLR2B)
-
Biorbyt antibodies for POLR2B
-
Boster Bio Antibodies for POLR2B
-
Custom Antibody ServicesProSci Antibodies for POLR2B
-
Santa Cruz Biotechnology (SCBT) Antibodies for POLR2B
- Sino Biological Antibodies for POLR2B
Protein products for research
- Search Origene for Purified Proteins, MassSpec and Protein Over-expression Lysates for POLR2B
- Origene Custom Protein Services for POLR2B
- Novus Biologicals proteins for POLR2B
Assay products for research
Domains & Families for POLR2B Gene
Gene Families for POLR2B Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for POLR2B Gene
- InterPro:
- Blocks:
-
- RNA polymerase Rpb2, domain 6
- RNA polymerase, beta subunit
- RNA polymerase Rpb2, domain 5
- RNA polymerase Rpb2, domain 4
- ProtoNet:
Suggested Antigen Peptide Sequences for POLR2B Gene
- GenScript: Design optimal peptide antigens:
-
- RpB2 (B2WTN6_HUMAN)
- DNA-directed RNA polymerase (C9J2Y9_HUMAN)
- DNA-directed RNA polymerase (C9J4M6_HUMAN)
- RNA polymerase II subunit B2 (RPB2_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P30876- Family:
-
- Belongs to the RNA polymerase beta chain family.
Function for POLR2B Gene
Molecular function for POLR2B Gene
- UniProtKB/Swiss-Prot Function:
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA-COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400; EC=2.7.7.6;.
- GENATLAS Biochemistry:
- polymerase II,RNA (DNA directed) large polypeptide B (140kDa)
Enzyme Numbers (IUBMB) for POLR2B Gene
Phenotypes From GWAS Catalog for POLR2B Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0001055 | contributes_to RNA polymerase II activity | ISS | -- |
| GO:0003677 | DNA binding | TAS,IEA | -- |
| GO:0003682 | chromatin binding | IEA | -- |
| GO:0003723 | RNA binding | HDA | 22658674 |
| GO:0003899 | contributes_to DNA-directed 5'-3' RNA polymerase activity | IBA | 21873635 |
Phenotypes for POLR2B Gene
- GenomeRNAi human phenotypes for POLR2B:
-
- Increased vaccinia virus (VACV) infection
- Decreased hepcidin::fluc mRNA expression
- no effect
- Decreased viability
- Reduced mammosphere formation
- Increased nuclear migration distance
- Decreased shRNA abundance (Z-score < -2)
- Inconclusive
- Decreased homologous recombination repair frequency
- Decreased shRNA abundance
- Increased shRNA abundance (Z-score > 2)
- Increased cell death HMECs cells
- Increased cell death in HCT116 cells
- Increased cell death in DLD-1 cells
- Increased circadian period length
- Increased ionizing radiation sensitivity
- Decreased cell number
- Decreased viability of wild-type and TP53 knockout cells, decreased TP53 protein expression ratio (wild-type / TP53 knockout cells)
- Increased cell death in HCC-1954 cells
- Decreased TP53 protein expression ratio (wild-type / TP53 knockout cells)
- Decreased G3BP1 protein expression and assembly in stress granules after arsenite stimulation
- Downregulation of NFkappaB pathway
Animal Models for research
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for POLR2B - Starting at $85 >
- * POLR2B CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for POLR2B
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for POLR2B
-
Santa Cruz Biotechnology (SCBT) CRISPR for POLR2B
miRNA for POLR2B Gene
- miRTarBase miRNAs that target POLR2B
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for POLR2B
- Browse OriGene Inhibitory RNA Products For POLR2B
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLR2B
Clone products for research
- Applied Biological Materials (abm): Clones for POLR2B - Starting at $45 >
- * POLR2B as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for POLR2B
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for POLR2B
No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for POLR2B Gene
Localization for POLR2B Gene
Subcellular locations from UniProtKB/Swiss-Prot for POLR2B Gene
- Nucleus.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytoskeleton | 2 |
| mitochondrion | 2 |
| cytosol | 2 |
| plasma membrane | 1 |
| extracellular | 1 |
| endoplasmic reticulum | 1 |
| golgi apparatus | 1 |
- Nucleoplasm (3)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000781 | colocalizes_with chromosome, telomeric region | IDA | 24270157 |
| GO:0005634 | nucleus | IEA,IDA | 9852112 |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005665 | RNA polymerase II, core complex | IDA | 9852112 |
| GO:0016020 | membrane | HDA | -- |
Pathways & Interactions for POLR2B Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Formation of HIV-1 elongation complex containing HIV-1 Tat |
.93
|
|
| 2 | RNA Polymerase II Transcription Initiation And Promoter Clearance |
Assembly of RNA Polymerase-II Initiation Complex
.91
Transcription of mRNA
.91
|
Protein Acetylation and Deacetylation
.51
Crosstalk Between CARM1 and ESRs
.39
Transcription Ligand-Dependent Transcription of Retinoid-Target genes
.32
|
| 3 | HIV Life Cycle |
.91
.64
|
.45
|
| 4 | Transcription-Coupled Nucleotide Excision Repair (TC-NER) | ||
| 5 | Signaling by FGFR2 in disease | ||
Pathways by source for POLR2B Gene
1 BioSystems pathway for POLR2B Gene
66 Reactome pathways for POLR2B Gene
2 KEGG pathways for POLR2B Gene
3 GeneGo (Thomson Reuters) pathways for POLR2B Gene
- ATP/ITP metabolism
- DNA damage_Role of Brca1 and Brca2 in DNA repair
- Transcription Ligand-Dependent Transcription of Retinoid-Target genes
17 Qiagen pathways for POLR2B Gene
- AHR Pathway
- Androgen Signaling
- Assembly of RNA Polymerase-II Initiation Complex
- CREB Pathway
- Crosstalk Between CARM1 and ESRs
Interacting Proteins for POLR2B Gene
Selected Interacting proteins:
ENSP00000370625
P30876-RPB2_HUMAN
for POLR2B Gene via
STRING
MINT
IID
GPS-Prot Interaction Network for POLR2B
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000398 | mRNA splicing, via spliceosome | TAS | -- |
| GO:0006283 | transcription-coupled nucleotide-excision repair | TAS | -- |
| GO:0006351 | transcription, DNA-templated | TAS | -- |
| GO:0006366 | transcription by RNA polymerase II | TAS | -- |
| GO:0006367 | transcription initiation from RNA polymerase II promoter | TAS | -- |
No data available for SIGNOR curated interactions for POLR2B Gene
Drugs & Compounds for POLR2B Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Magnesium | Approved, Experimental, Investigational | Pharma | 0 | |||
| Cytidine triphosphate | Experimental | Pharma | 0 | |||
| Guanosine triphosphate | Experimental | Pharma | 0 | |||
| Thymidine 5'-triphosphate | Experimental | Pharma | 0 | |||
| Uridine triphosphate | Experimental, Investigational | Pharma | 0 |
| Name | Synonyms | Role | CAS Number | PubChem IDs | PubMed IDs | |
|---|---|---|---|---|---|---|
| pyrophosphate |
|
14000-31-8 |
|
(3) ApexBio Compounds for POLR2B Gene
| Compound | Action | Cas Number |
|---|---|---|
| Actinomycin D | RNA polymerase inhibitor | 50-76-0 |
| PSI-6206 | Inhibitor of HCV RNA polymerase,potent and selective | 863329-66-2 |
| PSI-7977 | Antiviral agents for chronic HCV infection | 1190307-88-0 |
Transcripts for POLR2B Gene
mRNA/cDNA for POLR2B Gene
- 3 REFSEQ mRNAs :
- 11 NCBI additional mRNA sequence :
- 12 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000314595 3785 nts (view in UCSC)
- ENST00000381227 4108 nts (view in UCSC)
- ENST00000431623 3666 nts (view in UCSC)
- ENST00000433463 539 nts (view in UCSC)
- ENST00000441246 3839 nts (view in UCSC)
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for POLR2B - Starting at $85 >
- * POLR2B CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for POLR2B
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for POLR2B
-
Santa Cruz Biotechnology (SCBT) CRISPR for POLR2B
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for POLR2B
- Browse OriGene Inhibitory RNA Products For POLR2B
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLR2B
Clone products for research
- Applied Biological Materials (abm): Clones for POLR2B - Starting at $45 >
- * POLR2B as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for POLR2B
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for POLR2B
| ExUns: | 1a | · | 1b | · | 1c | ^ | 2a | · | 2b | ^ | 3a | · | 3b | ^ | 4a | · | 4b | ^ | 5a | · | 5b | · | 5c | ^ | 6a | · | 6b | ^ | 7a | · | 7b | · | 7c | ^ | 8 | ^ | 9a | · | 9b | ^ | 10a | · | 10b | ^ | 11a | · | 11b | · | 11c | ^ | 12a | · |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP2: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP3: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP4: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP5: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP6: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP7: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP8: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP9: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP10: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP11: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP12: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP13: | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||||
| SP14: | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||||
| SP15: |
| ExUns: | 12b | ^ | 13 | ^ | 14 | ^ | 15 | ^ | 16a | · | 16b | ^ | 17a | · | 17b | ^ | 18a | · | 18b | ^ | 19 | ^ | 20 | ^ | 21a | · | 21b | ^ | 22a | · | 22b | ^ | 23a | · | 23b | ^ | 24a | · | 24b | ^ | 25 | ^ | 26 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | |||||||||||||||||||||||||||||||||||||||||||
| SP2: | |||||||||||||||||||||||||||||||||||||||||||
| SP3: | |||||||||||||||||||||||||||||||||||||||||||
| SP4: | |||||||||||||||||||||||||||||||||||||||||||
| SP5: | |||||||||||||||||||||||||||||||||||||||||||
| SP6: | |||||||||||||||||||||||||||||||||||||||||||
| SP7: | |||||||||||||||||||||||||||||||||||||||||||
| SP8: | |||||||||||||||||||||||||||||||||||||||||||
| SP9: | |||||||||||||||||||||||||||||||||||||||||||
| SP10: | |||||||||||||||||||||||||||||||||||||||||||
| SP11: | |||||||||||||||||||||||||||||||||||||||||||
| SP12: | |||||||||||||||||||||||||||||||||||||||||||
| SP13: | |||||||||||||||||||||||||||||||||||||||||||
| SP14: | |||||||||||||||||||||||||||||||||||||||||||
| SP15: |
Relevant External Links for POLR2B Gene
- GeneLoc Exon Structure for
- POLR2B
Expression for POLR2B Gene
mRNA expression in embryonic tissues and stem cells from LifeMap Discovery
- Brain (Nervous System)
-
Ovary (Reproductive System)
- Primary Oocyte Primary Follicle
-
Gonad
- Primary Oocyte Primary Follicle
- Gut Tube (Gastrointestinal Tract)
- Neural Tube (Nervous System)
- Kidney (Urinary System)
- Thymus (Hematopoietic System)
- Eye (Sensory Organs)
-
Stomach (Gastrointestinal Tract)
This gene is overexpressed in Lymph node (17.1) and Peripheral blood mononuclear cells (9.2).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for POLR2B Gene
- Elite partner
Transcriptomic regulation report from SPP (The Signaling Pathways Project) for POLR2B
SOURCE GeneReport for Unigene cluster for POLR2B Gene:
Hs.602757Evidence on tissue expression from TISSUES for POLR2B Gene
- Nervous system(4.9)
- Skin(4.6)
- Liver(4.5)
- Muscle(2.8)
- Blood(2.7)
- Eye(2.5)
- Kidney(2.3)
- Heart(2.2)
- Intestine(2.2)
- Lung(2.1)
Primer products for research
-
OriGene qPCR primer pairs for POLR2B
-
OriGene qPCR primer pairs and template standards for POLR2B
Gene Expression Assays for research
No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for POLR2B Gene
Orthologs for POLR2B Gene
This gene was present in the common ancestor of eukaryotes.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| Platypus (Ornithorhynchus anatinus) |
Mammalia | -- 31 |
|
OneToMany | |
| -- 31 |
|
OneToMany | |||
| Oppossum (Monodelphis domestica) |
Mammalia | POLR2B 31 |
|
OneToOne | |
| Chimpanzee (Pan troglodytes) |
Mammalia | POLR2B 30 31 |
|
OneToOne | |
| Dog (Canis familiaris) |
Mammalia | POLR2B 30 31 |
|
OneToOne | |
| Cow (Bos Taurus) |
Mammalia | POLR2B 30 31 |
|
OneToOne | |
| Mouse (Mus musculus) |
Mammalia | Polr2b 30 17 31 |
|
OneToOne | |
| Rat (Rattus norvegicus) |
Mammalia | Polr2b 30 |
|
||
| Chicken (Gallus gallus) |
Aves | POLR2B 30 31 |
|
OneToOne | |
| Lizard (Anolis carolinensis) |
Reptilia | POLR2B 31 |
|
OneToOne | |
| Tropical Clawed Frog (Silurana tropicalis) |
Amphibia | polr2b 30 |
|
||
| Str.15006 30 |
|
||||
| African clawed frog (Xenopus laevis) |
Amphibia | MGC68569 30 |
|
||
| Zebrafish (Danio rerio) |
Actinopterygii | polr2b 30 31 |
|
OneToOne | |
| Dr.16327 30 |
|
||||
| Rainbow Trout (Oncorhynchus mykiss) |
Actinopterygii | Omy.13862 30 |
|
||
| Fruit Fly (Drosophila melanogaster) |
Insecta | RpII140 30 31 32 |
|
OneToOne | |
| RpIII128 32 |
|
|
|||
| RpI135 32 |
|
|
|||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP003648 30 |
|
||
| Worm (Caenorhabditis elegans) |
Secernentea | C26E6.4 32 |
|
|
|
| rpb-2 30 31 |
|
OneToOne | |||
| F09F7.3 32 |
|
|
|||
| F14B4.3 32 |
|
|
|||
| Baker's yeast (Saccharomyces cerevisiae) |
Saccharomycetes | RPB2 30 31 33 33 |
|
OneToMany | |
| K. Lactis Yeast (Kluyveromyces lactis) |
Saccharomycetes | KLLA0B05577g 30 |
|
||
| A. gosspyii yeast (Eremothecium gossypii) |
Saccharomycetes | AGOS_AFR404C 30 |
|
||
| Thale Cress (Arabidopsis thaliana) |
eudicotyledons | NRPB2 30 |
|
||
| Alicante grape (Vitis vinifera) |
eudicotyledons | Vvi.3309 30 |
|
||
| Rice (Oryza sativa) |
Liliopsida | Os03g0646800 30 |
|
||
| Os.25542 30 |
|
||||
| Barley (Hordeum vulgare) |
Liliopsida | Hv.4411 30 |
|
||
| Wheat (Triticum aestivum) |
Liliopsida | Ta.12287 30 |
|
||
| Sea Squirt (Ciona savignyi) |
Ascidiacea | CSA.1138 31 |
|
OneToOne | |
| Fission Yeast (Schizosaccharomyces pombe) |
Schizosaccharomycetes | rpb2 30 |
|
||
| Bread mold (Neurospora crassa) |
Ascomycetes | NCU05120 30 |
|
||
| Sea Vase (Ciona intestinalis) |
Ascidiacea | Cin.11524 30 |
|
- Species where no ortholog for POLR2B was found in the sources mined by GeneCards:
-
- Actinobacteria (Mycobacterium tuberculosis)
- Alpha proteobacteria (Wolbachia pipientis)
- Amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- Beta proteobacteria (Neisseria meningitidis)
- Chromalveolata (Phytophthora infestans)
- Common water flea (Daphnia pulex)
- Corn (Zea mays)
- E. coli (Escherichia coli)
- Filamentous fungi (Aspergillus nidulans)
- Firmicute Bacteria (Streptococcus pneumoniae)
- Green Algae (Chlamydomonas reinhardtii)
- Honey Bee (Apis mellifera)
- Loblloly Pine (Pinus taeda)
- Malaria Parasite (Plasmodium falciparum)
- Medicago Trunc (Medicago Truncatula)
- Moss (Physcomitrella patens)
- Orangutan (Pongo pygmaeus)
- Pig (Sus scrofa)
- Rice Blast Fungus (Magnaporthe grisea)
- Schistosome Parasite (Schistosoma mansoni)
- Sea Anemone (Nematostella vectensis)
- Sea Urchin (Strongylocentrotus purpuratus)
- Sorghum (Sorghum bicolor)
- Soybean (Glycine max)
- Stem Rust Fungus (Puccinia graminis)
- Sugarcane (Saccharum officinarum)
- Tomato (Lycopersicon esculentum)
- Toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
Evolution for POLR2B Gene
- ENSEMBL:
- Gene Tree for POLR2B (if available)
- TreeFam:
- Gene Tree for POLR2B (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for POLR2B: view image
Paralogs for POLR2B Gene
Variants for POLR2B Gene
Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for POLR2B Gene
| SNP ID | Clinical significance and condition | Chr 04 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| 916568 | Likely Pathogenic: Tracheoesophageal fistula | 57,017,210(+) |
C/T NM_000938.3(POLR2B):c.2123C>T (p.Ala708Val) |
MISSENSE |
All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| dgv5214n100 | CNV | gain | 25217958 |
Residual Variation Intolerance Score:
3.05% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
0.89;
18.44% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for POLR2B Gene
- SNPedia medical, phenotypic, and genealogical associations of SNPs for
- POLR2B
SNP Genotyping and Copy Number Assays for research
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for POLR2B Gene
Disorders for POLR2B Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| fusariosis |
|
|
| eumycotic mycetoma |
|
|
| dermatomycosis |
|
|
| tinea nigra |
|
|
| basidiobolomycosis |
|
|
Search POLR2B in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for POLR2B
- Genetic Association Database
- (GAD)
- Human Genome Epidemiology Navigator
- (HuGE)
- Atlas
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
- Open Targets Platform
- Open Targets Platform
No data available for UniProtKB/Swiss-Prot and Genatlas for POLR2B Gene
Publications for POLR2B Gene
- Primary structure of the second largest subunit of human RNA polymerase II (or B). (PMID: 1518060) Acker J … Kédinger C (Journal of molecular biology 1992) 2 3 4
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41
- Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk. (PMID: 19237606) Michiels S … Benhamou S (Carcinogenesis 2009) 3 41
- Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. (PMID: 17998332) Lee JH … Skalnik DG (Molecular and cellular biology 2008) 3 4
- Polymorphism discovery in 62 DNA repair genes and haplotype associations with risks for lung and head and neck cancers. (PMID: 17494052) Michiels S … Benhamou S (Carcinogenesis 2007) 3 41
ExternalLinks
Products for POLR2B Gene
- Custom Antibody ServicesOriGene Antibodies for POLR2B
- Browse OriGene ELISA Kits
- Custom Assay Services
- Search Origene for Purified Proteins, MassSpec and Protein Over-expression Lysates for POLR2B
- Origene Custom Protein Services for POLR2B
- Origene shrna, sirna, and RNAi products in human, mouse, rat for POLR2B
- Browse OriGene Inhibitory RNA Products For POLR2B
- OriGene qPCR primer pairs for POLR2B
- OriGene qPCR primer pairs and template standards for POLR2B
- OriGene CRISPR knockouts for POLR2B
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For POLR2B
- Sino Biological Human cDNA Clone for POLR2B
- Browse Sino Biological Recombinant Proteins
- Browse Sino Biological Cell Lysates
- Sino Biological Antibodies for POLR2B
- Browse Sino Biological Assays
- Browse ELISA Kits
- Browse CRO Services
- High-throughput Antibody Production Service
- Protein Production Service
- Epitope Tag Antibodies & Reporter Protein Antibodies
- Lentiviral ORF Clones
- Novus Biologicals Antibodies for POLR2B
- Novus Biologicals proteins for POLR2B
- Browse Antibodies
- Browse Peptides and Proteins
- Browse Lysates
- Browse ELISA Kits
- Abcam antibodies for POLR2B
- Find your target
- Browse Primary Antibodies
- Browse Conjugated Primary Antibodies
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- Browse ELISA Kits
- Browse Matched Antibody Pairs
- Browse Proteins and Peptides
- Search Knockout (KO) Validated Antibodies
- Browse Monoclonal Antibodies
- Browse Recombinant Antibodies
- Invitrogen Antibodies for POLR2B (AFLGC-POLR2B)
- ApexBio compounds for POLR2B
- VectorBuilder Custom and pre-made DNA vectors for POLR2B (ie. gene expression, shRNA knockdown, CRISPR)
- VectorBuilder Viral vectors for POLR2B (ie. lentivirus, AAV, adenovirus)
- VectorBuilder CRISPR vectors for POLR2B (ie. knockout, knockin, CRISPRa, CRISPRi)
- VectorBuilder Virus packaging for POLR2B CRISPR vectors (ie. lentivirus, AAV, adenovirus)
- VectorBuilder Stable cell line generation for POLR2B
- antibodies-online: Show 19 available POLR2B Antibodies ranked by validation data
- Compare Top POLR2B Antibodies
- antibodies-online: Search results for available POLR2B related products ranked by validation data
- antibodies-online: Search results for available POLR2B related products ranked by validation data
- Santa Cruz Biotechnology (SCBT) Antibodies for POLR2B
- Search Santa Cruz Biotechnology (SCBT) for POLR2B siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for POLR2B
- Custom Antibody ServicesProSci Antibodies for POLR2B
- Search for POLR2B Proteins at ProSci
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for POLR2B
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for POLR2B
- Search GeneCopoeia for miRNA 3'UTR Target clone products for POLR2B
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLR2B
- Boster Bio Antibodies for POLR2B
- Browse Boster Bio ELISA Kits
- Biorbyt antibodies for POLR2B
Sources for POLR2B Gene
- (1) GeneCards
- (2) HGNC
- (3) NCBI Entrez Gene
- (4) UniProtKB/Swiss-Prot
- (5) Ensembl
- (6) OMIM® (Updated: 05-Mar-2021)
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) Cell Signaling Technology
- (12) GO
- (13) UniProtKB/TrEMBL
- (14) InterPro
- (15) ProtoNet
- (16) Blocks
- (17) MGI
- (18) IUBMB
- (19) KEGG
- (20) MINT
- (21) STRING
- (22) IntAct
- (23) Novoseek
- (24) PharmGKB
- (25) DrugBank
- (26) HMDB
- (27) ASD
- (28) SAGE
- (29) SOURCE
- (30) HomoloGene
- (31) PanEnsembl
- (32) euGenes
- (33) SGD
- (34) FlyBase
- (35) WormBase
- (36) Pseudogene
- (37) DGV
- (38) dbSNP
- (39) GenAtlas
- (40) HGMD
- (41) GAD
- (42) BGMUT
- (43) HuGE
- (44) Atlas
- (45) GenBank
- (46) HORDE
- (47) HUGE
- (48) IMGT
- (49) Leiden
- (50) miRBase
- (51) DME
- (52) OriGene
- (53) PubMed
- (54) R&D Systems
- (55) TGDB
- (56) Tocris
- (57) Abcam
- (58) Novus Biologicals
- (59) ProSpec
- (60) Sino Biological
- (61) GenScript
- (62) Qiagen
- (63) OCA
- (64) Proteopedia
- (65) MOPED
- (66) neXtProt
- (67) Reactome
- (68) GeneGo (Thomson Reuters)
- (69) DISEASES
- (70) SIMAP
- (71) GenomeRNAi
- (72) LifeMap
- (73) miRTarBase
- (74) MalaCards
- (75) Invitrogen
- (76) BitterDB
- (77) ESI-BIO
- (78) RefSeq
- (79) BioSystems
- (80) MaxQB
- (81) IUPHAR
- (82) BioGPS
- (83) Illumina
- (84) COMPARTMENTS
- (85) HOMER
- (86) PaxDb
- (87) ApexBio
- (88) Addgene
- (89) antibodies-online
- (90) CYP
- (91) NONCODE
- (92) TreeFam
- (93) PathCards
- (94) GeneReviews
- (95) GeneTex
- (96) Taconic Biosciences
- (97) GTEx
- (98) ProteomicsDB
- (99) SCBT
- (100) DGIdb
- (101) ClinicalTrials
- (102) RVIS
- (103) SIGNOR
- (104) diseasecard
- (105) NIH Rare Diseases
- (106) Orphanet
- (107) UMLS
- (108) GTR
- (109) Disease Ontology
- (110) MedlinePlus Genetics
- (111) MeSH
- (112) MedlinePlus
- (113) CDC
- (114) NINDS
- (115) NCBI Bookshelf
- (116) ClinVar
- (117) Gene Damage Index
- (118) HPO
- (119) UDN
- (120) VISTA
- (121) FANTOM5
- (122) ENCODE
- (123) ProSci
- (124) Horizon
- (125) The Signaling Pathways Project (SPP)
- (126) IID
- (127) VectorBuilder
- (128) SNPedia
- (129) BRCA Exchange
- (130) CIViC
- (131) ProteoGenix
- (132) dbSUPER
- (133) TISSUES
- (134) Gene ORGANizer
- (135) abm
- (136) Human Protein Atlas
- (137) GWAS Catalog
- (138) Monarch Initiative
- (139) DataMed
- (140) HumanCyc
- (141) UCNEbase
- (142) EPDnew
- (143) Applied Biosystems
- (144) GlyConnect
- (145) Aminode
- (146) GeneCopoeia
- (147) GPS-Prot
- (148) RNAcentral
- (149) LncRNADisease
- (150) LOVD
- (151) miR2Disease
- (152) DiseaseEnhancer
- (153) Boster Bio
- (154) Biorbyt
- (155) Biocompare
- (156) Mastermind
- (157) CTD^2 Dashboard
- (158) CraniofacialAtlas
- (159) GeneImprint
- (160) Synthego
- (161) ENA
- (162) GtRNAdb
- (163) LncBase
- (164) LncBook
- (165) LNCipedia
- (166) Modomics
- (167) PDBe
- (168) Rfam
- (169) SILVA
- (170) snOPY
- (171) SRPDB
- (172) GENCODE
- (173) TarBase
- (174) Open Targets Platform
- (175) 5SRRNADB
- (176) snoDB
- (177) MirGeneDB