POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] See more...

Aliases for POLE3 Gene

Aliases for POLE3 Gene

  • DNA Polymerase Epsilon 3, Accessory Subunit 2 3 5
  • CHRAC17 2 3 4
  • Polymerase (DNA Directed), Epsilon 3 (P17 Subunit) 2 3
  • Chromatin Accessibility Complex 17 KDa Protein 3 4
  • Polymerase (DNA) Epsilon 3, Accessory Subunit 2 3
  • Chromatin Accessibility Complex Subunit 2 2 3
  • DNA Polymerase Epsilon P17 Subunit 2 3
  • DNA Polymerase Epsilon Subunit P17 3 4
  • DNA Polymerase Epsilon Subunit 3 3 4
  • Arsenic Transactivated Protein 2 3
  • Histone Fold Protein CHRAC17 2 3
  • DNA Polymerase II Subunit 3 3 4
  • HuCHRAC17 3 4
  • CHARAC17 2 3
  • CHRAC-17 3 4
  • CHRAC2 2 3
  • AsTP 3 4
  • P17 2 3
  • Polymerase (DNA Directed), Epsilon 3, Accessory Subunit 3
  • Chromatin Accessibility Complex 17 2
  • Arsenic-Transactivated Protein 4
  • EC 2.7.7.7 51
  • POLE3 5
  • YBL1 3
  • Ybl1 2

External Ids for POLE3 Gene

Previous GeneCards Identifiers for POLE3 Gene

  • GC09M106967
  • GC09M107901
  • GC09M109623
  • GC09M111545
  • GC09M113249
  • GC09M115209
  • GC09M116169
  • GC09M085777

Summaries for POLE3 Gene

Entrez Gene Summary for POLE3 Gene

  • POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]

GeneCards Summary for POLE3 Gene

POLE3 (DNA Polymerase Epsilon 3, Accessory Subunit) is a Protein Coding gene. Diseases associated with POLE3 include Macular Degeneration, Age-Related, 2. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Transcription-Coupled Nucleotide Excision Repair (TC-NER). Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and DNA-directed DNA polymerase activity.

UniProtKB/Swiss-Prot Summary for POLE3 Gene

  • Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed:10801849).

Gene Wiki entry for POLE3 Gene

Additional gene information for POLE3 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for POLE3 Gene

Genomics for POLE3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for POLE3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH09J113409 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 600.7 +0.0 49 3 BRCA1 SP1 CREB1 GATAD2A PRDM10 ZNF629 ZNF512 RFX1 LEF1 SOX13 C9orf43 POLE3 RGS3 PRPF4
GH09J113372 Promoter/Enhancer 2.4 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 11.5 +32.7 32749 11.9 ARHGAP35 SP1 GATAD2A REST TFE3 ZNF512 IKZF1 NFKBIZ ZNF692 POLR2A HDHD3 lnc-ALAD-2 piR-30350-065 RGS3 PRPF4 C9orf43 POLE3 RNF183 ALAD CDC26
GH09J113577 Promoter/Enhancer 2 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 11.1 -169.5 -169474 6.3 SP1 HNRNPL PRDM10 IKZF1 POLR2A BACH1 TARDBP JUND FOXA1 LARP7 RGS3 C9orf43 POLE3 ALAD RNF183 ENSG00000237073 lnc-C9orf43-6 ZNF618
GH09J113563 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 10 -154.8 -154757 3.1 HNRNPL ZBTB33 IKZF1 JUND MTA2 ZIC2 PKNOX1 POLR2A MTA1 REST RGS3 lnc-C9orf43-6 C9orf43 RNF183 POLE3 CDC26 PRPF4 ALAD ENSG00000237073 ZNF618
GH09J113339 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 9.9 +70.6 70648 1.8 BRCA1 SP1 HNRNPL GATAD2A PRDM10 REST ZNF629 TFE3 RFX1 SIX5 WDR31 lnc-BSPRY-1 PRPF4 CDC26 BSPRY RNF183 ALAD C9orf43 POLE3 HDHD3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around POLE3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for POLE3

Top Transcription factor binding sites by QIAGEN in the POLE3 gene promoter:
  • AP-1
  • GR
  • GR-alpha

Genomic Locations for POLE3 Gene

Genomic Locations for POLE3 Gene
chr9:113,407,235-113,410,749
(GRCh38/hg38)
Size:
3,515 bases
Orientation:
Minus strand
chr9:116,169,515-116,173,029
(GRCh37/hg19)
Size:
3,515 bases
Orientation:
Minus strand

Genomic View for POLE3 Gene

Genes around POLE3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
POLE3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for POLE3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for POLE3 Gene

Proteins for POLE3 Gene

  • Protein details for POLE3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NRF9-DPOE3_HUMAN
    Recommended name:
    DNA polymerase epsilon subunit 3
    Protein Accession:
    Q9NRF9
    Secondary Accessions:
    • Q5W0U1
    • Q8N758
    • Q8NCE5
    • Q9NR32

    Protein attributes for POLE3 Gene

    Size:
    147 amino acids
    Molecular mass:
    16860 Da
    Quaternary structure:
    • Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC.

neXtProt entry for POLE3 Gene

Selected DME Specific Peptides for POLE3 Gene

Q9NRF9:
  • TPLKEALEAYRR
  • KGKKEASEQKKKDKDKK
  • EEQDKSR

Post-translational modifications for POLE3 Gene

  • Ubiquitination at Lys31, Lys62, and Lys86
  • Modification sites at PhosphoSitePlus

Other Protein References for POLE3 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for POLE3 Gene

Gene Families for POLE3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • FDA approved drug targets
  • Predicted intracellular proteins

Protein Domains for POLE3 Gene

InterPro:
Blocks:
  • Transcription factor CBF/NF-Y/archaeal histone
ProtoNet:

Suggested Antigen Peptide Sequences for POLE3 Gene

GenScript: Design optimal peptide antigens:
  • DNA polymerase epsilon subunit p17 (DPOE3_HUMAN)
genes like me logo Genes that share domains with POLE3: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for POLE3 Gene

Function for POLE3 Gene

Molecular function for POLE3 Gene

UniProtKB/Swiss-Prot Function:
Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed:10801849).

Enzyme Numbers (IUBMB) for POLE3 Gene

Phenotypes From GWAS Catalog for POLE3 Gene

Gene Ontology (GO) - Molecular Function for POLE3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003887 DNA-directed DNA polymerase activity TAS 10801849
GO:0005515 protein binding IPI 10801849
GO:0031490 chromatin DNA binding IBA 21873635
GO:0046982 protein heterodimerization activity IEA --
genes like me logo Genes that share ontologies with POLE3: view
genes like me logo Genes that share phenotypes with POLE3: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLE3

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for POLE3 Gene

Localization for POLE3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for POLE3 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for POLE3 gene
Compartment Confidence
nucleus 5
extracellular 1
cytoskeleton 1
mitochondrion 1
cytosol 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoli (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for POLE3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 10801849
GO:0005654 nucleoplasm TAS --
GO:0005671 Ada2/Gcn5/Ada3 transcription activator complex IDA 18838386
GO:0008622 epsilon DNA polymerase complex IBA,IDA 10801849
GO:0008623 CHRAC IBA 21873635
genes like me logo Genes that share ontologies with POLE3: view

Pathways & Interactions for POLE3 Gene

PathCards logo

SuperPathways for POLE3 Gene

SuperPathway Contained pathways
1 Telomere C-strand (Lagging Strand) Synthesis
.48
2 Regulation of activated PAK-2p34 by proteasome mediated degradation
.78
3 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
4 DNA Double-Strand Break Repair
5 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
genes like me logo Genes that share pathways with POLE3: view

Pathways by source for POLE3 Gene

1 GeneGo (Thomson Reuters) pathway for POLE3 Gene
  • Cell cycle Transition and termination of DNA replication

Gene Ontology (GO) - Biological Process for POLE3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0006260 DNA replication TAS 10801849
GO:0006270 DNA replication initiation TAS --
GO:0006272 leading strand elongation IBA 21873635
GO:0006974 cellular response to DNA damage stimulus IBA 21873635
genes like me logo Genes that share ontologies with POLE3: view

No data available for SIGNOR curated interactions for POLE3 Gene

Drugs & Compounds for POLE3 Gene

(7) Drugs for POLE3 Gene - From: DrugBank, ApexBio, and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cladribine Approved, Investigational Pharma Target, inhibitor Apoptosis inducer in CLL cells 105
Clofarabine Approved, Investigational Pharma Antimetabolite,inhibit DNA polymerase and ribonucleotide reductase 167
Phosphoric acid Approved Pharma 0
dATP Experimental Pharma Agonist, Full agonist 0
dctp Experimental Pharma 0

(1) Additional Compounds for POLE3 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(ho)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • PYROphosphATE
14000-31-8

(1) ApexBio Compounds for POLE3 Gene

Compound Action Cas Number
Clofarabine Antimetabolite,inhibit DNA polymerase and ribonucleotide reductase 123318-82-1
genes like me logo Genes that share compounds with POLE3: view

Drug Products

Transcripts for POLE3 Gene

mRNA/cDNA for POLE3 Gene

2 REFSEQ mRNAs :
13 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLE3

Alternative Splicing Database (ASD) splice patterns (SP) for POLE3 Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c · 5d · 5e · 5f
SP1: -
SP2: - -
SP3:
SP4: -
SP5: - -
SP6:
SP7:

Relevant External Links for POLE3 Gene

GeneLoc Exon Structure for
POLE3

Expression for POLE3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for POLE3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for POLE3 Gene

This gene is overexpressed in Bone (23.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for POLE3 Gene



Protein tissue co-expression partners for POLE3 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for POLE3

SOURCE GeneReport for Unigene cluster for POLE3 Gene:

Hs.108112

mRNA Expression by UniProt/SwissProt for POLE3 Gene:

Q9NRF9-DPOE3_HUMAN
Tissue specificity: Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Evidence on tissue expression from TISSUES for POLE3 Gene

  • Nervous system(4.8)
  • Lung(4.3)
  • Skin(2.7)
  • Intestine(2.5)
  • Eye(2.2)
  • Liver(2)
genes like me logo Genes that share expression patterns with POLE3: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for POLE3 Gene

Orthologs for POLE3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for POLE3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia POLE3 30 31
  • 100 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia POLE3 30 31
  • 93.88 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia POLE3 31
  • 92 (a)
OneToOne
Mouse
(Mus musculus)
Mammalia Pole3 30 17 31
  • 91.53 (n)
OneToMany
Gm21827 31
  • 77 (a)
OneToMany
Dog
(Canis familiaris)
Mammalia POLE3 30 31
  • 91.38 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Pole3 30
  • 90.98 (n)
Chicken
(Gallus gallus)
Aves POLE3 30 31
  • 77.87 (n)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia pole3 30
  • 77.51 (n)
Str.10053 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.32726 30
Zebrafish
(Danio rerio)
Actinopterygii pole3 30 31
  • 74.25 (n)
OneToOne
zgc73351 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.9305 30
Fruit Fly
(Drosophila melanogaster)
Insecta Chrac-14 30 31
  • 58.5 (n)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes HAP3 31
  • 20 (a)
OneToMany
Species where no ortholog for POLE3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for POLE3 Gene

ENSEMBL:
Gene Tree for POLE3 (if available)
TreeFam:
Gene Tree for POLE3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for POLE3: view image

Paralogs for POLE3 Gene

(1) SIMAP similar genes for POLE3 Gene using alignment to 1 proteins:

  • DPOE3_HUMAN
genes like me logo Genes that share paralogs with POLE3: view

No data available for Paralogs for POLE3 Gene

Variants for POLE3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for POLE3 Gene

SNP ID Clinical significance and condition Chr 09 pos Variation AA Info Type
rs34852828 - p.Asp126Ala
rs35933626 - p.Glu135Asp
rs36023979 - p.Thr83Ala

Additional dbSNP identifiers (rs#s) for POLE3 Gene

Structural Variations from Database of Genomic Variants (DGV) for POLE3 Gene

Variant ID Type Subtype PubMed ID
esv3621540 CNV gain 21293372

Variation tolerance for POLE3 Gene

Residual Variation Intolerance Score: 61.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.29; 6.56% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for POLE3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
POLE3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for POLE3 Gene

Disorders for POLE3 Gene

MalaCards: The human disease database

(1) MalaCards diseases for POLE3 Gene - From: DISEASES

Disorder Aliases PubMed IDs
macular degeneration, age-related, 2
  • armd2
- elite association - COSMIC cancer census association via MalaCards
Search POLE3 in MalaCards View complete list of genes associated with diseases
genes like me logo Genes that share disorders with POLE3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for POLE3 Gene

Publications for POLE3 Gene

  1. HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins. (PMID: 10880450) Poot RA … Varga-Weisz PD (The EMBO journal 2000) 2 3 4
  2. Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon. (PMID: 10801849) Li Y … Linn S (The Journal of biological chemistry 2000) 2 3 4
  3. DNA sequence and analysis of human chromosome 9. (PMID: 15164053) Humphray SJ … Dunham I (Nature 2004) 3 4
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4

Products for POLE3 Gene

Sources for POLE3 Gene