Aliases for POLA2 Gene

Aliases for POLA2 Gene

  • DNA Polymerase Alpha 2, Accessory Subunit 2 3 5
  • DNA Polymerase Alpha 70 KDa Subunit 2 3 4
  • DNA Polymerase Alpha Subunit B 2 3 4
  • Polymerase (DNA Directed), Alpha 2 (70kD Subunit) 2 3
  • Polymerase (DNA) Alpha 2, Accessory Subunit 2 3
  • Polymerase (DNA Directed), Alpha 2, Accessory Subunit 3
  • Polymerase (DNA-Directed), Alpha (70kD) 3
  • FLJ21662 2
  • POLA2 5

External Ids for POLA2 Gene

Previous GeneCards Identifiers for POLA2 Gene

  • GC11P067545
  • GC11P066711
  • GC11P065279
  • GC11P064804
  • GC11P064786
  • GC11P065029
  • GC11P061356

Summaries for POLA2 Gene

GeneCards Summary for POLA2 Gene

POLA2 (DNA Polymerase Alpha 2, Accessory Subunit) is a Protein Coding gene. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Mitotic G1-G1/S phases. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and DNA-directed DNA polymerase activity. An important paralog of this gene is ENSG00000285816.

UniProtKB/Swiss-Prot Summary for POLA2 Gene

  • Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).

Gene Wiki entry for POLA2 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for POLA2 Gene

Genomics for POLA2 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for POLA2 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around POLA2 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for POLA2

Top Transcription factor binding sites by QIAGEN in the POLA2 gene promoter:
  • AML1a
  • c-Myc
  • E47
  • Max1
  • Meis-1a
  • Meis-1b
  • MyoD
  • NF-1
  • NF-1/L
  • SEF-1 (1)

Genomic Locations for POLA2 Gene

Latest Assembly
chr11:65,261,917-65,306,269
(GRCh38/hg38)
Size:
44,353 bases
Orientation:
Plus strand

Previous Assembly
chr11:65,029,433-65,066,156
(GRCh37/hg19 by Entrez Gene)
Size:
36,724 bases
Orientation:
Plus strand

chr11:65,029,233-65,073,060
(GRCh37/hg19 by Ensembl)
Size:
43,828 bases
Orientation:
Plus strand

Genomic View for POLA2 Gene

Genes around POLA2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
POLA2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for POLA2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for POLA2 Gene

Proteins for POLA2 Gene

  • Protein details for POLA2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q14181-DPOA2_HUMAN
    Recommended name:
    DNA polymerase alpha subunit B
    Protein Accession:
    Q14181
    Secondary Accessions:
    • B4DNB4
    • Q9BPV3

    Protein attributes for POLA2 Gene

    Size:
    598 amino acids
    Molecular mass:
    65948 Da
    Quaternary structure:
    • Component of the alpha DNA polymerase complex (also known as the alpha DNA polymerase-primase complex) consisting of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and primase complex subunits PRIM1 and PRIM2 respectively (PubMed:9705292). Within the complex, POLA1 directly interacts with PRIM2/p58 (By similarity).

    Three dimensional structures from OCA and Proteopedia for POLA2 Gene

    Alternative splice isoforms for POLA2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for POLA2 Gene

Post-translational modifications for POLA2 Gene

  • Phosphorylated in a cell cycle-dependent manner, in G2/M phase.
  • Ubiquitination at Lys72, Lys292, Lys160, Lys194, Lys207, Lys212, Lys500, and Lys564
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for POLA2 Gene

Domains & Families for POLA2 Gene

Gene Families for POLA2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for POLA2 Gene

InterPro:
Blocks:
  • DNA polymerase alpha, subunit B N-terminal
ProtoNet:

Suggested Antigen Peptide Sequences for POLA2 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ60312, highly similar to DNA polymerase subunit alpha B (B4DNB4_HUMAN)
  • DNA polymerase alpha 70 kDa subunit (DPOA2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q14181

UniProtKB/Swiss-Prot:

DPOA2_HUMAN :
  • The N-terminal 240 amino acids are sufficient to mediate complex formation.
  • Belongs to the DNA polymerase alpha subunit B family.
Domain:
  • The N-terminal 240 amino acids are sufficient to mediate complex formation.
Family:
  • Belongs to the DNA polymerase alpha subunit B family.
genes like me logo Genes that share domains with POLA2: view

Function for POLA2 Gene

Molecular function for POLA2 Gene

UniProtKB/Swiss-Prot Function:
Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).

Phenotypes From GWAS Catalog for POLA2 Gene

Gene Ontology (GO) - Molecular Function for POLA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003674 molecular_function ND --
GO:0003677 DNA binding IEA --
GO:0005515 protein binding IPI 32814053
genes like me logo Genes that share ontologies with POLA2: view
genes like me logo Genes that share phenotypes with POLA2: view

Animal Models for POLA2 Gene

MGI Knock Outs for POLA2:
  • Pola2 Pola2<tm1b(KOMP)Mbp>

miRNA for POLA2 Gene

miRTarBase miRNAs that target POLA2

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLA2

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for POLA2 Gene

Localization for POLA2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for POLA2 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for POLA2 gene
Compartment Confidence
nucleus 5
cytosol 4
cytoskeleton 2
extracellular 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (3)
  • Cytosol (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for POLA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
GO:0005658 alpha DNA polymerase:primase complex IBA,IDA 8026492
GO:0005829 cytosol IDA --
genes like me logo Genes that share ontologies with POLA2: view

Pathways & Interactions for POLA2 Gene

genes like me logo Genes that share pathways with POLA2: view

Pathways by source for POLA2 Gene

1 KEGG pathway for POLA2 Gene
3 GeneGo (Thomson Reuters) pathways for POLA2 Gene
  • Cell cycle Start of DNA replication in early S phase
  • Cell cycle Transition and termination of DNA replication
  • Transcription Ligand-dependent activation of the ESR1/SP pathway

Gene Ontology (GO) - Biological Process for POLA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0006260 DNA replication IEA,NAS 8223465
GO:0006269 DNA replication, synthesis of RNA primer IDA 8026492
GO:0006270 DNA replication initiation TAS --
GO:0032201 telomere maintenance via semi-conservative replication TAS --
genes like me logo Genes that share ontologies with POLA2: view

No data available for SIGNOR curated interactions for POLA2 Gene

Drugs & Compounds for POLA2 Gene

(2) Drugs for POLA2 Gene - From: DrugBank, ApexBio, DGIdb, and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Dacarbazine Approved, Investigational Pharma Target, other/unknown 198
Fludarabine Phosphate (Fludara) Pharma Inhibits STAT1 activation and DNA synthesis 0

(1) ApexBio Compounds for POLA2 Gene

Compound Action Cas Number
Fludarabine Phosphate (Fludara) Inhibits STAT1 activation and DNA synthesis 75607-67-9
genes like me logo Genes that share compounds with POLA2: view

Drug products for research

Transcripts for POLA2 Gene

mRNA/cDNA for POLA2 Gene

1 REFSEQ mRNAs :
12 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLA2

Alternative Splicing Database (ASD) splice patterns (SP) for POLA2 Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15a · 15b ^ 16 ^ 17a · 17b · 17c ^ 18a ·
SP1: - - - -
SP2:
SP3: - - -
SP4:
SP5:
SP6: - -
SP7: -

ExUns: 18b
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:

Relevant External Links for POLA2 Gene

GeneLoc Exon Structure for
POLA2

Expression for POLA2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for POLA2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for POLA2 Gene

This gene is overexpressed in Pancreas (30.5), Heart (18.9), and Fetal ovary (8.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for POLA2 Gene



Protein tissue co-expression partners for POLA2 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for POLA2

SOURCE GeneReport for Unigene cluster for POLA2 Gene:

Hs.201897

Evidence on tissue expression from TISSUES for POLA2 Gene

  • Lung(4.5)
  • Heart(4.1)
  • Nervous system(3.9)
genes like me logo Genes that share expression patterns with POLA2: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for POLA2 Gene

Orthologs for POLA2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for POLA2 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia POLA2 30 31
  • 99.72 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia POLA2 30 31
  • 86.64 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia POLA2 30 31
  • 85.84 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Pola2 30
  • 82.83 (n)
Mouse
(Mus musculus)
Mammalia Pola2 30 17 31
  • 81.77 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 80 (a)
OneToMany
-- 31
  • 74 (a)
OneToMany
-- 31
  • 47 (a)
OneToMany
Oppossum
(Monodelphis domestica)
Mammalia POLA2 31
  • 72 (a)
OneToOne
Chicken
(Gallus gallus)
Aves LOC100859759 30
  • 59.87 (n)
POLA2 31
  • 53 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia POLA2 31
  • 72 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia pola2 30
  • 64.59 (n)
Str.6808 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.4593 30
Zebrafish
(Danio rerio)
Actinopterygii pola2 30 31
  • 62.21 (n)
OneToOne
wufb48f05 30
Fruit Fly
(Drosophila melanogaster)
Insecta DNApol-alpha73 30 31
  • 47.62 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004392 30
  • 45.82 (n)
Worm
(Caenorhabditis elegans)
Secernentea div-1 31
  • 23 (a)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes POL12 31 33
  • 21 (a)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons POLA2 30
  • 50.25 (n)
Rice
(Oryza sativa)
Liliopsida Os12g0242900 30
  • 48.98 (n)
Species where no ortholog for POLA2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for POLA2 Gene