This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding mult... See more...

Aliases for PKMYT1 Gene

Aliases for PKMYT1 Gene

  • Protein Kinase, Membrane Associated Tyrosine/Threonine 1 2 3 5
  • Membrane-Associated Tyrosine- And Threonine-Specific Cdc2-Inhibitory Kinase 2 3 4
  • Protein Phosphatase 1, Regulatory Subunit 126 2 3
  • Myt1 Kinase 3 4
  • MYT1 3 4
  • EC 2.7.11.1 4
  • PPP1R126 3

External Ids for PKMYT1 Gene

Previous GeneCards Identifiers for PKMYT1 Gene

  • GC16M003043
  • GC16M003023
  • GC16M002962
  • GC16M003019
  • GC16M003020
  • GC16M002995

Summaries for PKMYT1 Gene

Entrez Gene Summary for PKMYT1 Gene

  • This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

GeneCards Summary for PKMYT1 Gene

PKMYT1 (Protein Kinase, Membrane Associated Tyrosine/Threonine 1) is a Protein Coding gene. Among its related pathways are Oocyte meiosis and Cell cycle. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is EIF2AK4.

UniProtKB/Swiss-Prot Summary for PKMYT1 Gene

  • Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

Gene Wiki entry for PKMYT1 Gene

Additional gene information for PKMYT1 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PKMYT1 Gene

Genomics for PKMYT1 Gene

GeneHancer (GH) Regulatory Elements for PKMYT1 Gene

Promoters and enhancers for PKMYT1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PKMYT1 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PKMYT1

Top Transcription factor binding sites by QIAGEN in the PKMYT1 gene promoter:
  • AP-1
  • c-Myc
  • E2F
  • E2F-1
  • E2F-2
  • E2F-3a
  • E2F-4
  • E2F-5
  • USF-1
  • USF1

Genomic Locations for PKMYT1 Gene

Genomic Locations for PKMYT1 Gene
chr16:2,968,024-2,980,539
(GRCh38/hg38)
Size:
12,516 bases
Orientation:
Minus strand
chr16:3,018,025-3,030,540
(GRCh37/hg19)
Size:
12,516 bases
Orientation:
Minus strand

Genomic View for PKMYT1 Gene

Genes around PKMYT1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PKMYT1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PKMYT1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PKMYT1 Gene

Proteins for PKMYT1 Gene

  • Protein details for PKMYT1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99640-PMYT1_HUMAN
    Recommended name:
    Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
    Protein Accession:
    Q99640
    Secondary Accessions:
    • B3KUN8
    • B4DXD4
    • D3DUA4
    • F8W164
    • I3L1V2
    • O14731
    • Q7LE24
    • Q8TCM9

    Protein attributes for PKMYT1 Gene

    Size:
    499 amino acids
    Molecular mass:
    54521 Da
    Quaternary structure:
    • Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.
    SequenceCaution:
    • Sequence=CAD28540.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PKMYT1 Gene

    Alternative splice isoforms for PKMYT1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PKMYT1 Gene

Post-translational modifications for PKMYT1 Gene

  • Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
  • Ubiquitination at Lys42 and Lys163
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for PKMYT1 Gene

Domains & Families for PKMYT1 Gene

Gene Families for PKMYT1 Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins
  • Predicted membrane proteins

Suggested Antigen Peptide Sequences for PKMYT1 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ60243, highly similar to Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC 2.7.11.1) (A6NHV6_HUMAN)
  • cDNA FLJ61000, highly similar to Membrane-associated tyrosine- andthreonine-specific cdc2-inhibitory kinase (EC 2.7.11.1) (B4DZM6_HUMAN)
  • Myt1 kinase (PMYT1_HUMAN)
  • PKMYT1 protein (Q0IJ49_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q99640

UniProtKB/Swiss-Prot:

PMYT1_HUMAN :
  • The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Domain:
  • The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
genes like me logo Genes that share domains with PKMYT1: view

Function for PKMYT1 Gene

Molecular function for PKMYT1 Gene

UniProtKB/Swiss-Prot Function:
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.

Enzyme Numbers (IUBMB) for PKMYT1 Gene

Phenotypes From GWAS Catalog for PKMYT1 Gene

Gene Ontology (GO) - Molecular Function for PKMYT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IEA,IBA 21873635
GO:0004674 protein serine/threonine kinase activity IBA,IEA 21873635
GO:0005515 protein binding IPI 10373560
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with PKMYT1: view
genes like me logo Genes that share phenotypes with PKMYT1: view

Animal Model Products

  • Taconic Biosciences Mouse Models for PKMYT1

CRISPR Products

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PKMYT1 Gene

Localization for PKMYT1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PKMYT1 Gene

Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PKMYT1 gene
Compartment Confidence
nucleus 5
endoplasmic reticulum 4
cytosol 4
golgi apparatus 4
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (3)
  • Nucleoli (1)
  • Nucleoplasm (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PKMYT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm TAS --
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with PKMYT1: view

Pathways & Interactions for PKMYT1 Gene

genes like me logo Genes that share pathways with PKMYT1: view

Pathways by source for PKMYT1 Gene

1 Cell Signaling Technology pathway for PKMYT1 Gene

SIGNOR curated interactions for PKMYT1 Gene

Inactivates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for PKMYT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity TAS 9001210
GO:0000086 G2/M transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS 9001210
GO:0006468 protein phosphorylation IEA --
GO:0007049 cell cycle IEA --
genes like me logo Genes that share ontologies with PKMYT1: view

Drugs & Compounds for PKMYT1 Gene

(2) Drugs for PKMYT1 Gene - From: DrugBank and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Pore Blocker, Potentiation 0

(1) Additional Compounds for PKMYT1 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Full agonist, Agonist, Partial agonist, Antagonist, Gating inhibitor 58-64-0
genes like me logo Genes that share compounds with PKMYT1: view

Transcripts for PKMYT1 Gene

mRNA/cDNA for PKMYT1 Gene

4 REFSEQ mRNAs :
15 NCBI additional mRNA sequence :
20 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for PKMYT1 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3a · 3b · 3c · 3d · 3e · 3f ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b ^ 8a · 8b · 8c · 8d ^ 9 ^
SP1: - - - - -
SP2: - - - - - -
SP3: - - - - - - -
SP4: - - - - - - -
SP5: - - - -
SP6:
SP7: - - - - -
SP8: - - - -
SP9: - - -
SP10: -
SP11: - - -
SP12: - -
SP13:

ExUns: 10 ^ 11
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:

Relevant External Links for PKMYT1 Gene

GeneLoc Exon Structure for
PKMYT1

Expression for PKMYT1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PKMYT1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PKMYT1 Gene

This gene is overexpressed in Testis (x19.2).

Protein differential expression in normal tissues from HIPED for PKMYT1 Gene

This gene is overexpressed in Testis (62.9) and Urine (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for PKMYT1 Gene



Protein tissue co-expression partners for PKMYT1 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for PKMYT1

SOURCE GeneReport for Unigene cluster for PKMYT1 Gene:

Hs.732385

Evidence on tissue expression from TISSUES for PKMYT1 Gene

  • Nervous system(4.5)
  • Thyroid gland(4)
genes like me logo Genes that share expression patterns with PKMYT1: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for PKMYT1 Gene

Orthologs for PKMYT1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PKMYT1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PKMYT1 31 30
  • 99.67 (n)
OneToOne
cow
(Bos Taurus)
Mammalia PKMYT1 31 30
  • 88.64 (n)
OneToOne
dog
(Canis familiaris)
Mammalia PKMYT1 31 30
  • 88.14 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pkmyt1 30
  • 85.96 (n)
mouse
(Mus musculus)
Mammalia Pkmyt1 17 31 30
  • 85.51 (n)
oppossum
(Monodelphis domestica)
Mammalia PKMYT1 31
  • 79 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PKMYT1 31
  • 72 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PKMYT1 31
  • 43 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia pkmyt1 30
  • 55.26 (n)
African clawed frog
(Xenopus laevis)
Amphibia MGC68436 30
zebrafish
(Danio rerio)
Actinopterygii pkmyt1 31 30
  • 52.58 (n)
OneToOne
Dr.11509 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.7419 30
fruit fly
(Drosophila melanogaster)
Insecta Myt1 31 32
  • 30 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea wee-1.1 31 32
  • 23 (a)
OneToMany
wee-1.3 31 32
  • 23 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SWE1 33 31
  • 11 (a)
Species where no ortholog for PKMYT1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PKMYT1 Gene

ENSEMBL:
Gene Tree for PKMYT1 (if available)
TreeFam:
Gene Tree for PKMYT1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for PKMYT1: view image

Paralogs for PKMYT1 Gene

Paralogs for PKMYT1 Gene

(10) SIMAP similar genes for PKMYT1 Gene using alignment to 10 proteins:

  • PMYT1_HUMAN
  • A6NHV6_HUMAN
  • B4DZM6_HUMAN
  • I3L136_HUMAN
  • I3L1H7_HUMAN
  • I3L2S4_HUMAN
  • I3L3P0_HUMAN
  • I3L4K3_HUMAN
  • I3L4Y0_HUMAN
  • Q0IJ49_HUMAN
genes like me logo Genes that share paralogs with PKMYT1: view

Variants for PKMYT1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for PKMYT1 Gene

SNP ID Clinical significance and condition Chr 16 pos Variation AA Info Type
708191 Benign: not provided 2,975,551(-) G/A SYNONYMOUS_VARIANT
rs10546 - p.Val445Ala
rs35192104 - p.Arg246His
rs4149796 - p.Arg140Cys
rs4149800 - p.Pro417Arg

Additional dbSNP identifiers (rs#s) for PKMYT1 Gene

Structural Variations from Database of Genomic Variants (DGV) for PKMYT1 Gene

Variant ID Type Subtype PubMed ID
nsv509588 CNV insertion 20534489
nsv510673 CNV deletion 20534489
nsv571246 CNV loss 21841781
nsv827525 CNV gain 20364138
nsv952906 CNV deletion 24416366

Variation tolerance for PKMYT1 Gene

Residual Variation Intolerance Score: 34% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.45; 64.10% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PKMYT1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PKMYT1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PKMYT1 Gene

Disorders for PKMYT1 Gene

Additional Disease Information for PKMYT1

No disorders were found for PKMYT1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PKMYT1 Gene

Publications for PKMYT1 Gene

  1. The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex. (PMID: 9001210) Liu F … Piwnica-Worms H (Molecular and cellular biology 1997) 2 3 4 23 54
  2. The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase. (PMID: 12606722) Passer BJ … Telerman A (Proceedings of the National Academy of Sciences of the United States of America 2003) 2 3 23 54
  3. Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate. (PMID: 12738781) Nakajima H … Nishida E (The Journal of biological chemistry 2003) 3 4 23 54
  4. Overproduction of human Myt1 kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-cyclin B1 complexes. (PMID: 10373560) Liu F … Piwnica-Worms H (Molecular and cellular biology 1999) 3 4 23 54
  5. The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts with Cdc2 complexes and is required for inhibition of G(2)/M progression. (PMID: 10504341) Wells NJ … Hunter T (Journal of cell science 1999) 3 4 23 54

Products for PKMYT1 Gene

Sources for PKMYT1 Gene