Aliases for PKM Gene
Aliases for PKM Gene
External Ids for PKM Gene
- HGNC: 9021
- Entrez Gene: 5315
- Ensembl: ENSG00000067225
- OMIM: 179050
- UniProtKB: P14618
Previous HGNC Symbols for PKM Gene
- PKM2
Previous GeneCards Identifiers for PKM Gene
- GC15M072492
Summaries for PKM Gene
-
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
GeneCards Summary for PKM Gene
PKM (Pyruvate Kinase M1/2) is a Protein Coding gene. Diseases associated with PKM include Pyruvate Kinase Deficiency Of Red Cells and Acute Diarrhea. Among its related pathways are Glucose metabolism and Glycosaminoglycan metabolism. Gene Ontology (GO) annotations related to this gene include MHC class II protein complex binding. An important paralog of this gene is PKLR.
UniProtKB/Swiss-Prot for PKM Gene
-
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Additional gene information for PKM Gene
- Monarch Initiative
- Search for PKM at DataMed
- Search for PKM at HumanCyc
No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PKM Gene
Genomics for PKM Gene
GeneHancer (GH) Regulatory Elements for PKM Gene
Promoters and enhancers for PKM Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data dump
GeneHancers around PKM on UCSC Golden Path with GeneCards custom track
Regulatory Element Products
Genomic Locations for PKM Gene
Genomic Locations for PKM Gene
- chr15:72,199,029-72,231,822
- (GRCh38/hg38)
- Size:
- 32,794 bases
- Orientation:
- Minus strand
- chr15:72,491,370-72,524,164
- (GRCh37/hg19)
Genomic View for PKM Gene
Genes around PKM on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 15q23 by Ensembl
- 15q23 by Entrez Gene
- 15q23 by HGNC
PKM Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for PKM Gene
Proteins for PKM Gene
-
Protein details for PKM Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P14618-KPYM_HUMAN
- Recommended name:
- Pyruvate kinase PKM
- Protein Accession:
- P14618
- A6NFK3
- B2R5N8
- B3KRY0
- B4DFX8
- B4DUU6
- P14786
- Q53GK4
- Q96E76
- Q9BWB5
- Q9UCV6
- Q9UPF2
Protein attributes for PKM Gene
- Size:
- 531 amino acids
- Molecular mass:
- 57937 Da
- Cofactor:
- Name=K(+); Xref=ChEBI:CHEBI:29103;
- Cofactor:
- Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Quaternary structure:
-
- Monomer and homotetramer. Exists as a monomer in the absence of D-fructose 1,6-bisphosphate (FBP), and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia. Interacts (isoform M2, but not isoform M1) with TRIM35; this interaction prevents FGFR1-dependent tyrosine phosphorylation (PubMed:25263439).
- Miscellaneous:
-
- There are 4 isozymes of pyruvate kinase in mammals (L, R, M1, M2) encoded by 2 different genes: PKLR and PKM. The L and R isozymes are generated from the PKLR by differential splicing of RNA; the M1 and M2 forms are produced from the PKM gene by differential splicing. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells.
- SequenceCaution:
-
- Sequence=BAG57589.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Three dimensional structures from OCA and Proteopedia for PKM Gene
Alternative splice isoforms for PKM Gene
- UniProtKB/Swiss-Prot:
Protein Expression for PKM Gene
Selected DME Specific Peptides for PKM Gene
- P14618:
-
- VDMVFASFIR
- GKARGFFK
- AAMADTFLEH
- EIPAEKV
- LEHMCRL
- ICATQMLESM
- QTARQAHLYRG
- GPASRSV
- GAVEASFKC
- IAREAEAA
- KGVNLPG
- RYRPRAPIIAVTRN
- VANAVLDG
- IKIISKIE
- DDGLISL
- SDGIMVARGD
- VDLPAVS
- MVARGDLG
- NFSHGTH
- LWLDYKNICKVVEVGSK
- ATESFASDPI
- EVENGGSLGS
- ATQMLESMI
- NAVLDGAD
- PRPTRAE
- LDTKGPEIR
- TEVENGG
- GSKKGVN
- RLNFSHG
- MLSGETA
- TGWRPGSG
Post-translational modifications for PKM Gene
- Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
- FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
- ISGylated.
- Under hypoxia, hydroxylated by EGLN3.
- Ubiquitination at isoforms=266, isoforms=289, Lys125, isoforms=3, 2151, Lys166, Lys188, Lys207, Lys266, Lys270, isoforms=3, 2367, Lys475, and isoforms=3, 2498
- Modification sites at PhosphoSitePlus
Other Protein References for PKM Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- R&D Systems Antibodies for PKM (PKM2)
-
Custom Antibody ServicesOriGene Antibodies for PKM
- AM50349PU-N
- AM50349PU-S
- AP08210PU-N
- TA190271
- TA190270
- TA190269
- TA190268
- TA190267
- TA190266
- TA190265
- TA190264
- TA190262
- TA190260
- TA190259
- TA347018
- TA309804
- TA351941
- TA347826
- TA344601
- TA344600
- TA336903
- TA327597
- TA326808
- TA324317
- TA322979
- TA322978
- R1108HRPS
- R1108BS
- R1108
- AP53318PU-N
- AP26075PU-S
- AP26075PU-N
- AP26067PU-S
- AP26067PU-N
- AP22592PU-N
- AP13828PU-N
- AP13827PU-N
- AP13595PU-N
- AP08813PU-N
- Novus Biologicals Antibodies for PKM
-
Abcam antibodies for PKM
-
Cloud-Clone Corp. Antibodies for PKM
- Invitrogen Antibodies for PKM
- antibodies-online: Search results for available PKM related products ranked by validation data
- GeneTex PKM antibody for PKM
-
Santa Cruz Biotechnology (SCBT) Antibodies for PKM
Protein Products
- R&D Systems Proteins and Enzymes for PKM (PKM2)
- Search Origene for MassSpec and Protein Over-expression Lysates for PKM
- Origene Custom Protein Services for PKM
- Sino Biological Cell Lysates for PKM
- Sino Biological Recombinant Proteins for PKM
- ProSpec Recombinant Proteins for PKM
-
Cloud-Clone Corp. Proteins for PKM
- antibodies-online: Search results for available PKM related products ranked by validation data
- Search GeneTex for Proteins for PKM
-
ProSci Proteins for PKM
-
Abcam proteins for PKM
Assay Products
-
Cloud-Clone Corp. Assay Kits for PKM
- antibodies-online: Search results for available PKM related products ranked by validation data
Domains & Families for PKM Gene
Gene Families for PKM Gene
- Human Protein Atlas (HPA):
-
- Cancer-related genes
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for PKM Gene
- InterPro:
- Blocks:
- ProtoNet:
Suggested Antigen Peptide Sequences for PKM Gene
- GenScript: Design optimal peptide antigens:
Graphical View of Domain Structure for InterPro Entry
P14618- Family:
-
- Belongs to the pyruvate kinase family.
Function for PKM Gene
Molecular function for PKM Gene
- UniProtKB/Swiss-Prot BiophysicochemicalProperties:
- Kinetic parameters: KM=2.7 mM for phosphoenolpyruvate (at 32 degrees Celsius, pH 8.0) {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723}; KM=0.17 mM for phosphoenolpyruvate (in the presence of 2 mM D- fructose 1,6-bisphosphate (FBP), at 32 degrees Celsius, pH 8.0) {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723}; KM=0.34 mM for ADP (at 32 degrees Celsius, pH 8.0) {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723}; KM=0.24 mM for ADP (in the presence of 2 mM FBP, at 32 degrees Celsius, pH 8.0) {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723}; KM=0.13 mM for phosphoenolpyruvate (in the presence of 2 mM FBP, at 25 degrees Celsius) {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723}; KM=0.63 mM for ADP (in the presence of 2 mM FBP, at 25 degrees Celsius) {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723}; pH dependence: Optimum pH for T3 binding is 6.0-6.5. Increase in pH causes T3 binding to drop, does not bind T3 above pH 9.0 or below pH 5.0. {ECO:0000269 PubMed:15996096, ECO:0000269 PubMed:1854723};
- UniProtKB/Swiss-Prot CatalyticActivity:
- ATP + pyruvate = ADP + phosphoenolpyruvate.
- UniProtKB/Swiss-Prot EnzymeRegulation:
- Isoform M2 is allosterically activated by D-fructose 1,6-bisphosphate (FBP). Inhibited by oxalate and 3,3,5-triiodo-L-thyronine (T3). The activity of the tetrameric form is inhibited by PML. Selective binding to tyrosine-phosphorylated peptides releases the allosteric activator FBP, leading to inhibition of PKM enzymatic activity, this diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. Glycolytic flux are highly dependent on de novo biosynthesis of serine and glycine, and serine is a natural ligand and allosteric activator of isoform M2.
- UniProtKB/Swiss-Prot Function:
- Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Enzyme Numbers (IUBMB) for PKM Gene
Phenotypes From GWAS Catalog for PKM Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000287 | magnesium ion binding | IEA | -- |
| GO:0003723 | RNA binding | HDA,IDA | 22658674 |
| GO:0003824 | catalytic activity | IEA | -- |
| GO:0004743 | pyruvate kinase activity | IEA,TAS | 2040271 |
| GO:0005515 | protein binding | IPI | 12620389 |
Phenotypes for PKM Gene
- MGI mutant phenotypes for PKM:
- inferred from 4 alleles
- GenomeRNAi human phenotypes for PKM:
-
- Increased viability
- Increased vaccinia virus (VACV) infection
- shRNA abundance <= 50%
- Increased shRNA abundance (Z-score > 2)
- Decreased shRNA abundance (Z-score < -2)
- Decreased viability
- Increased HPV18 LCR reporter activity
- Decreased shRNA abundance
- Increased cell death HMECs cells
- Synthetic lethal with vaccinia virus (VACV) infection
- Decreased viability after gemcitabine stimulation
- Decreased BPV1 E2 protein expression
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
- Cyagen custom Knockout/knockin (KOKI) mouse models for PKM
-
- Search ViGene Biosciences for PKM
CRISPR Products
-
OriGene CRISPR knockouts for PKM
- genomics-online: gRNA clones - Search results for available PKM gene related products
- Applied Biological Materials CRISPR for PKM
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for PKM
- GenScript: Design CRISPR guide RNA sequences for PKM
miRNA for PKM Gene
- miRTarBase miRNAs that target PKM
-
- hsa-mir-133b (MIRT001815)
- hsa-mir-133a-3p (MIRT001816)
- hsa-mir-326 (MIRT005520)
- hsa-mir-122-5p (MIRT023396)
- hsa-mir-30a-5p (MIRT028653)
- hsa-mir-1301-3p (MIRT036045)
- hsa-mir-935 (MIRT036697)
- hsa-mir-877-3p (MIRT036965)
- hsa-mir-423-5p (MIRT038155)
- hsa-mir-296-3p (MIRT038436)
- hsa-mir-766-3p (MIRT039032)
- hsa-mir-769-5p (MIRT039183)
- hsa-mir-18a-3p (MIRT040961)
- hsa-mir-484 (MIRT041665)
- hsa-mir-423-3p (MIRT042646)
- hsa-mir-324-3p (MIRT042876)
- hsa-mir-328-3p (MIRT043754)
- hsa-mir-330-3p (MIRT043843)
- hsa-mir-320a (MIRT044585)
- hsa-mir-125b-5p (MIRT046067)
- hsa-mir-222-3p (MIRT046680)
- hsa-mir-221-3p (MIRT046929)
- hsa-mir-30c-5p (MIRT047865)
- hsa-mir-92a-3p (MIRT049024)
- hsa-mir-23a-3p (MIRT050390)
- hsa-mir-1260b (MIRT052783)
- hsa-mir-3187-3p (MIRT052808)
- hsa-mir-218-5p (MIRT439960)
- hsa-mir-6730-5p (MIRT470931)
- hsa-mir-4665-5p (MIRT470932)
- hsa-mir-1275 (MIRT470933)
- hsa-mir-6515-5p (MIRT470934)
- hsa-mir-4727-3p (MIRT470935)
- hsa-mir-4472 (MIRT470936)
- hsa-mir-4447 (MIRT470937)
- hsa-mir-7847-3p (MIRT470938)
- hsa-mir-6880-5p (MIRT470939)
- hsa-mir-6745 (MIRT470940)
- hsa-mir-363-5p (MIRT470941)
- hsa-mir-5010-5p (MIRT470942)
- hsa-mir-4525 (MIRT470943)
- hsa-mir-7111-5p (MIRT470944)
- hsa-mir-6870-5p (MIRT470945)
- hsa-mir-5698 (MIRT470946)
- hsa-mir-4723-5p (MIRT470947)
- hsa-mir-625-5p (MIRT470948)
- hsa-mir-4668-5p (MIRT470949)
- hsa-mir-6822-3p (MIRT470950)
- hsa-mir-504-3p (MIRT470951)
- hsa-mir-6851-5p (MIRT470952)
- hsa-mir-3689d (MIRT470953)
- hsa-mir-6797-5p (MIRT470954)
- hsa-mir-1249-5p (MIRT470955)
- hsa-mir-3202 (MIRT470956)
- hsa-mir-4533 (MIRT470957)
- hsa-mir-3116 (MIRT470958)
- hsa-mir-1254 (MIRT470959)
- hsa-mir-3659 (MIRT470960)
- hsa-mir-6804-5p (MIRT470961)
- hsa-mir-17-3p (MIRT470962)
- hsa-mir-4254 (MIRT470963)
- hsa-mir-3129-5p (MIRT470964)
- hsa-mir-199b-3p (MIRT470965)
- hsa-mir-199a-3p (MIRT470966)
- hsa-mir-4650-5p (MIRT470967)
- hsa-mir-1296-3p (MIRT470968)
- hsa-mir-4694-3p (MIRT470969)
- hsa-mir-338-3p (MIRT732802)
- hsa-let-7a-5p (MIRT732965)
- hsa-mir-369-3p (MIRT733295)
- hsa-mir-184 (MIRT735440)
- hsa-mir-1205 (MIRT735987)
- hsa-mir-1238-5p (MIRT736335)
- hsa-mir-1285-3p (MIRT737032)
- hsa-mir-1286 (MIRT737060)
- hsa-mir-1302 (MIRT737267)
- hsa-mir-153-5p (MIRT737886)
- hsa-mir-1587 (MIRT737920)
- hsa-mir-185-3p (MIRT738214)
- hsa-mir-185-5p (MIRT738254)
- hsa-mir-19a-3p (MIRT738748)
- hsa-mir-19b-3p (MIRT738759)
- hsa-mir-3135b (MIRT740134)
- hsa-mir-3144-5p (MIRT740222)
- hsa-mir-3176 (MIRT740699)
- hsa-mir-3179 (MIRT740743)
- hsa-mir-3187-5p (MIRT740927)
- hsa-mir-3191-3p (MIRT740989)
- hsa-mir-330-5p (MIRT741333)
- hsa-mir-3620-5p (MIRT741748)
- hsa-mir-3652 (MIRT741898)
- hsa-mir-378g (MIRT742768)
- hsa-mir-3909 (MIRT742830)
- hsa-mir-3922-3p (MIRT742975)
- hsa-mir-3945 (MIRT743183)
- hsa-mir-4253 (MIRT743355)
- hsa-mir-4263 (MIRT743488)
- hsa-mir-4271 (MIRT743630)
- hsa-mir-4298 (MIRT743817)
- hsa-mir-4306 (MIRT743910)
- hsa-mir-432-3p (MIRT744059)
- hsa-mir-4430 (MIRT744336)
- hsa-mir-4436a (MIRT744438)
- hsa-mir-4481 (MIRT745116)
- hsa-mir-4486 (MIRT745185)
- hsa-mir-4492 (MIRT745296)
- hsa-mir-4498 (MIRT745377)
- hsa-mir-4505 (MIRT745535)
- hsa-mir-4534 (MIRT746057)
- hsa-mir-4644 (MIRT746382)
- hsa-mir-4648 (MIRT746469)
- hsa-mir-4651 (MIRT746565)
- hsa-mir-4652-5p (MIRT746604)
- hsa-mir-4656 (MIRT746658)
- hsa-mir-4675 (MIRT746887)
- hsa-mir-4722-5p (MIRT747618)
- hsa-mir-4725-3p (MIRT747711)
- hsa-mir-4731-5p (MIRT747945)
- hsa-mir-4741 (MIRT748124)
- hsa-mir-4745-5p (MIRT748187)
- hsa-mir-4747-5p (MIRT748263)
- hsa-mir-4758-5p (MIRT748485)
- hsa-mir-491-5p (MIRT749221)
- hsa-mir-5000-3p (MIRT749325)
- hsa-mir-5001-5p (MIRT749370)
- hsa-mir-5189-5p (MIRT749888)
- hsa-mir-5192 (MIRT749948)
- hsa-mir-5196-5p (MIRT750047)
- hsa-mir-552-3p (MIRT750880)
- hsa-mir-576-5p (MIRT751448)
- hsa-mir-5787 (MIRT751484)
- hsa-mir-6085 (MIRT751805)
- hsa-mir-608 (MIRT752002)
- hsa-mir-612 (MIRT752230)
- hsa-mir-637 (MIRT752694)
- hsa-mir-6732-5p (MIRT754080)
- hsa-mir-6752-5p (MIRT754648)
- hsa-mir-6756-5p (MIRT754809)
- hsa-mir-6758-5p (MIRT754882)
- hsa-mir-6766-5p (MIRT755117)
- hsa-mir-6780a-3p (MIRT755551)
- hsa-mir-6780b-5p (MIRT755642)
- hsa-mir-6810-5p (MIRT756665)
- hsa-mir-6813-5p (MIRT756741)
- hsa-mir-6829-5p (MIRT757111)
- hsa-mir-6842-5p (MIRT757382)
- hsa-mir-6852-3p (MIRT757631)
- hsa-mir-6856-5p (MIRT757728)
- hsa-mir-6860 (MIRT757861)
- hsa-mir-6862-5p (MIRT757891)
- hsa-mir-6894-5p (MIRT758785)
- hsa-mir-7110-5p (MIRT759008)
- hsa-mir-7154-3p (MIRT759235)
- hsa-mir-762 (MIRT759512)
- hsa-mir-764 (MIRT759534)
- hsa-mir-765 (MIRT759581)
- hsa-mir-766-5p (MIRT759652)
- hsa-mir-8077 (MIRT760142)
- hsa-mir-873-5p (MIRT760319)
- hsa-mir-92a-2-5p (MIRT760577)
miRNA Products
- Search ViGene Biosciences for PKM
Inhibitory RNA Products
- Origene RNAi, sirna, and shrna products in human, mouse, rat for PKM
- Browse OriGene Inhibitory RNA Products For PKM
- genomics-online: shRNA clones - Search results for available PKM gene related products
Clone Products
- Sino Biological Human cDNA Clone for PKM
- VectorBuilder custom plasmid, inducible vectors for PKM
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for PKM
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for PKM
- genomics-online: cdna clones - Search results for available PKM gene related products
- orf clones - Search results for available PKM gene related products
- Applied Biological Materials Clones for PKM
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
Cell Line Products
No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PKM Gene
Localization for PKM Gene
Subcellular locations from UniProtKB/Swiss-Prot for PKM Gene
- Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
| Compartment | Confidence |
|---|---|
| extracellular | 5 |
| mitochondrion | 5 |
| nucleus | 5 |
| cytosol | 5 |
| plasma membrane | 4 |
| cytoskeleton | 2 |
| endoplasmic reticulum | 1 |
- Cytosol (4)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005576 | extracellular region | TAS | -- |
| GO:0005634 | nucleus | IDA,IEA | 17308100 |
| GO:0005737 | cytoplasm | IDA,IEA | 18298799 |
| GO:0005739 | mitochondrion | HDA,IEA | 20833797 |
| GO:0005829 | cytosol | TAS | -- |
Pathways & Interactions for PKM Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Glucose metabolism |
.52
|
.01
|
| 2 | Innate Immune System |
.61
|
|
| 3 | Carbon metabolism | ||
| 4 | Metabolism |
.37
|
|
| 5 | Glucose / Energy Metabolism | ||
Pathways by source for PKM Gene
6 BioSystems pathways for PKM Gene
7 Reactome pathways for PKM Gene
1 PharmGKB pathway for PKM Gene
11 KEGG pathways for PKM Gene
2 GeneGo (Thomson Reuters) pathways for PKM Gene
1 R&D Systems pathway for PKM Gene
2 Cell Signaling Technology pathways for PKM Gene
UniProtKB/Swiss-Prot P14618-KPYM_HUMAN
- Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Interacting Proteins for PKM Gene
Selected Interacting proteins:
ENSP00000320171
P14618-KPYM_HUMAN
for PKM Gene via
STRING
MINT
IID
UniProtKB
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0001666 | response to hypoxia | IEA | -- |
| GO:0001889 | liver development | IEA | -- |
| GO:0006006 | glucose metabolic process | IEA | -- |
| GO:0006096 | glycolytic process | IEA | -- |
| GO:0006754 | ATP biosynthetic process | IEA | -- |
Drugs & Compounds for PKM Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Pyruvic acid | Approved, Investigational | Nutra | Target | 0 | ||
| Copper | Approved, Investigational | Pharma | Target | 202 | ||
| Carbon dioxide | Approved, Investigational, Vet_approved | Pharma | 0 | |||
| Potassium | Approved, Investigational | Pharma | 0 | |||
| Magnesium | Approved | Nutra | 0 |
| Name | Synonyms | Role | CAS Number | PubChem IDs | PubMed IDs | |
|---|---|---|---|---|---|---|
| ADP |
|
Full agonist, Agonist | 58-64-0 | |||
| dADP |
|
2793-06-8 |
|
|||
| dGDP |
|
3493-09-2 |
|
|||
| IDP |
|
86-04-4 |
|
|||
| inosine triphosphate |
|
132-06-9 |
|
(1) ApexBio Compounds for PKM Gene
| Compound | Action | Cas Number |
|---|---|---|
| DASA-58 | activator of pyruvate kinase M2 (PKM2) | 1203494-49-8 |
Transcripts for PKM Gene
mRNA/cDNA for PKM Gene
- (13) REFSEQ mRNAs :
- (25) Additional mRNA sequences :
- (3712) Selected AceView cDNA sequences:
- (25) Ensembl transcripts including schematic representations, and UCSC links where relevant :
Unigene Clusters for PKM Gene
- Pyruvate kinase, muscle:
- Representative Sequences:
CRISPR Products
-
OriGene CRISPR knockouts for PKM
- genomics-online: gRNA clones - Search results for available PKM gene related products
- Applied Biological Materials CRISPR for PKM
-
Vectors and viruses for KO, Activation, Repression, and more
-
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Clone Products
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-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for PKM
- genomics-online: cdna clones - Search results for available PKM gene related products
- orf clones - Search results for available PKM gene related products
- Applied Biological Materials Clones for PKM
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | · | 3c | ^ | 4a | · | 4b | ^ | 5a | · | 5b | ^ | 6a | · | 6b | ^ | 7a | · | 7b | · | 7c | · | 7d | ^ | 8a | · | 8b | · | 8c | · | 8d | ^ | 9 | ^ | 10 | ^ | 11a | · | 11b | · | 11c | ^ | 12a | · | 12b | ^ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | - | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||
| SP2: | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |||||||||||||||||||||||||||||||||||
| SP3: | - | - | - | - | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||
| SP4: | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||||
| SP5: | - | - | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||
| SP6: | - | - | ||||||||||||||||||||||||||||||||||||||||||||||||||
| SP7: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP8: | - | - | - | - | - | - | - | - | - | - | - | - | ||||||||||||||||||||||||||||||||||||||||
| SP9: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP10: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP11: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP12: | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||||
| SP13: |
| ExUns: | 13a | · | 13b | ^ | 14a | · | 14b | · | 14c | · | 14d | ^ | 15 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | |||||||||||
| SP2: | - | - | - | ||||||||||
| SP3: | - | - | |||||||||||
| SP4: | |||||||||||||
| SP5: | - | - | - | ||||||||||
| SP6: | |||||||||||||
| SP7: | |||||||||||||
| SP8: | |||||||||||||
| SP9: | |||||||||||||
| SP10: | |||||||||||||
| SP11: | |||||||||||||
| SP12: | |||||||||||||
| SP13: |
Expression for PKM Gene
mRNA expression in embryonic tissues and stem cells from LifeMap Discovery
- Brain (Nervous System)
- Neural Tube (Nervous System)
- Thymus (Hematopoietic System)
- Lung (Respiratory System)
- Smooth Muscle (Muscoskeletal System)
- Adipose (Muscoskeletal System)
- Bone (Muscoskeletal System)
- Kidney (Urinary System)
- Lower Urinary Tract (Urinary System)
-
Blood (Cardiovascular System)
- Double Negative 2 Thymocytes Thymus
This gene is overexpressed in Bone marrow stromal cell (9.5).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PKM Gene
NURSA nuclear receptor signaling pathways regulating expression of PKM Gene:
PKMSOURCE GeneReport for Unigene cluster for PKM Gene:
Hs.534770mRNA Expression by UniProt/SwissProt for PKM Gene:
P14618-KPYM_HUMAN
Tissue specificity:
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Evidence on tissue expression from TISSUES for PKM Gene
- Eye(5)
- Kidney(5)
- Liver(5)
- Skin(5)
- Nervous system(5)
- Blood(4.9)
- Intestine(4.9)
- Lung(4.9)
- Pancreas(4.9)
- Muscle(4.7)
- Bone(4.3)
- Heart(4.3)
- Stomach(4.3)
- Spleen(4)
- Thyroid gland(4)
- Lymph node(2.8)
- Adrenal gland(2.3)
- Bone marrow(2.3)
- Gall bladder(2.2)
Primer Products
-
OriGene qPCR primer pairs for PKM
- genomics-online: primer clones - Search results for available PKM gene related products
Gene Expression Assay Products
No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for PKM Gene
Orthologs for PKM Gene
This gene was present in the common ancestor of eukaryotes.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| chimpanzee (Pan troglodytes) |
Mammalia | PKM 34 33 |
|
OneToOne | |
| platypus (Ornithorhynchus anatinus) |
Mammalia | PKM 34 |
|
OneToOne | |
| cow (Bos Taurus) |
Mammalia | PKM2 34 |
|
OneToOne | |
| PKM 33 |
|
||||
| dog (Canis familiaris) |
Mammalia | -- 34 |
|
OneToMany | |
| PKM 33 |
|
||||
| -- 34 |
|
OneToMany | |||
| rat (Rattus norvegicus) |
Mammalia | Pkm 33 |
|
||
| mouse (Mus musculus) |
Mammalia | Pkm 33 16 34 |
|
||
| oppossum (Monodelphis domestica) |
Mammalia | -- 34 |
|
OneToMany | |
| -- 34 |
|
OneToMany | |||
| chicken (Gallus gallus) |
Aves | PKM 34 |
|
OneToOne | |
| PKM2 33 |
|
||||
| lizard (Anolis carolinensis) |
Reptilia | PKM 34 |
|
OneToOne | |
| tropical clawed frog (Silurana tropicalis) |
Amphibia | pkm 33 |
|
||
| Str.373 33 |
|
||||
| zebrafish (Danio rerio) |
Actinopterygii | pkma 33 34 |
|
||
| pkmb 34 |
|
OneToMany | |||
| pkm2 33 |
|
||||
| rainbow trout (Oncorhynchus mykiss) |
Actinopterygii | Omy.7227 33 |
|
||
| fruit fly (Drosophila melanogaster) |
Insecta | PyK 33 34 |
|
||
| CG2964 34 |
|
ManyToMany | |||
| CG7069 34 |
|
ManyToMany | |||
| CG7362 34 |
|
ManyToMany | |||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP004596 33 |
|
||
| worm (Caenorhabditis elegans) |
Secernentea | pyk-1 33 34 |
|
||
| pyk-2 34 |
|
ManyToMany | |||
| baker's yeast (Saccharomyces cerevisiae) |
Saccharomycetes | CDC19 33 34 36 |
|
||
| PYK2 34 |
|
ManyToMany | |||
| K. lactis yeast (Kluyveromyces lactis) |
Saccharomycetes | KLLA0F23397g 33 |
|
||
| A. gosspyii yeast (Ashbya gossypii) |
Saccharomycetes | AGOS_ADR368W 33 |
|
||
| thale cress (Arabidopsis thaliana) |
eudicotyledons | AT5G63680 33 |
|
||
| Alicante grape (Vitis vinifera) |
eudicotyledons | Vvi.1458 33 |
|
||
| rice (Oryza sativa) |
Liliopsida | Os04g0677500 33 |
|
||
| sea squirt (Ciona savignyi) |
Ascidiacea | -- 34 |
|
OneToMany | |
| fission yeast (Schizosaccharomyces pombe) |
Schizosaccharomycetes | pyk1 33 |
|
||
| bread mold (Neurospora crassa) |
Ascomycetes | NCU06075 33 |
|
||
| sea squirt (Ciona intestinalis) |
Ascidiacea | Cin.4710 33 |
|
- Species where no ortholog for PKM was found in the sources mined by GeneCards:
-
- Actinobacteria (Mycobacterium tuberculosis)
- African clawed frog (Xenopus laevis)
- alpha proteobacteria (Wolbachia pipientis)
- amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- barley (Hordeum vulgare)
- beta proteobacteria (Neisseria meningitidis)
- Chromalveolata (Phytophthora infestans)
- common water flea (Daphnia pulex)
- corn (Zea mays)
- E. coli (Escherichia coli)
- filamentous fungi (Aspergillus nidulans)
- Firmicute bacteria (Streptococcus pneumoniae)
- green algae (Chlamydomonas reinhardtii)
- honey bee (Apis mellifera)
- loblloly pine (Pinus taeda)
- malaria parasite (Plasmodium falciparum)
- medicago trunc (Medicago Truncatula)
- moss (Physcomitrella patens)
- orangutan (Pongo pygmaeus)
- pig (Sus scrofa)
- rice blast fungus (Magnaporthe grisea)
- schistosome parasite (Schistosoma mansoni)
- sea anemone (Nematostella vectensis)
- sea urchin (Strongylocentrotus purpuratus)
- sorghum (Sorghum bicolor)
- soybean (Glycine max)
- stem rust fungus (Puccinia graminis)
- sugarcane (Saccharum officinarum)
- tomato (Lycopersicon esculentum)
- toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- wheat (Triticum aestivum)
Paralogs for PKM Gene
(2) SIMAP similar genes for PKM Gene using alignment to 9 proteins:
Pseudogenes.org Pseudogenes for PKM Gene
Variants for PKM Gene
| SNP ID | Clin | Chr 15 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| rs11558368 | likely-benign, not specified | 72,202,513(-) | G/A | coding_sequence_variant, intron_variant, non_coding_transcript_variant, synonymous_variant | |
| rs879255367 | uncertain-significance, not specified | 72,208,664(-) | T/C | coding_sequence_variant, missense_variant, non_coding_transcript_variant | |
| rs10514 | benign, not specified | 72,209,800(-) | G/A | coding_sequence_variant, non_coding_transcript_variant, synonymous_variant | |
| rs1000107033 | -- | 72,209,197(-) | T/C | intron_variant | |
| rs1000246117 | -- | 72,200,374(-) | G/A | downstream_transcript_variant, genic_downstream_transcript_variant, intron_variant |
Residual Variation Intolerance Score:
18.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
0.84;
17.66% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for PKM Gene
SNP Genotyping and Copy Number Assay Products
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PKM Gene
Disorders for PKM Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| pyruvate kinase deficiency of red cells |
|
|
| acute diarrhea |
|
|
| pigmentation disease |
|
|
| atrichia with papular lesions |
|
|
| li-fraumeni syndrome |
|
|
Search PKM in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for PKM
No data available for UniProtKB/Swiss-Prot and Genatlas for PKM Gene
Publications for PKM Gene
- Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription. (PMID: 18191611) Lee J … Kim J (The international journal of biochemistry & cell biology 2008) 3 4 22 58
- Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein. (PMID: 18298799) Shimada N … Ishii S (Genes to cells : devoted to molecular & cellular mechanisms 2008) 3 4 22 58
- Pyruvate kinase M2 is a phosphotyrosine-binding protein. (PMID: 18337815) Christofk HR … Cantley LC (Nature 2008) 3 4 22 58
- Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death. (PMID: 17308100) Steták A … Ullrich A (Cancer research 2007) 3 4 25 58
- Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis. (PMID: 15996096) Dombrauckas JD … Mesecar AD (Biochemistry 2005) 3 4 22 58
Products for PKM Gene
- R&D Systems Antibodies for PKM (PKM2)
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- AM50349PU-N
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- TA344600
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- TA347826
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- TA309804
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- TA190259
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- ApexBio compounds for PKM
- Cyagen custom Knockout/knockin (KOKI) mouse models for PKM
- VectorBuilder custom plasmid, inducible vectors for PKM
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for PKM
- VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for PKM
- antibodies-online: Search results for available PKM related products ranked by validation data
- antibodies-online: Search results for available PKM related products ranked by validation data
- antibodies-online: Search results for available PKM related products ranked by validation data
- GeneTex PKM antibody for PKM
- Search GeneTex for Proteins for PKM
- Search ViGene Biosciences for PKM
- Santa Cruz Biotechnology (SCBT) Antibodies for PKM
- Search Santa Cruz Biotechnology (SCBT) for PKM siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for PKM
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- Custom Antibody Services
- ProSci Proteins for PKM
- genomics-online: cdna clones - Search results for available PKM gene related products
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- genomics-online: gRNA clones - Search results for available PKM gene related products
- genomics-online: primer clones - Search results for available PKM gene related products
- genomics-online: shRNA clones - Search results for available PKM gene related products
Sources for PKM Gene
- (1) GeneCards
- (2) HGNC
- (3) EntrezGene
- (4) Swiss-Prot
- (5) Ensembl
- (6)