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Aliases for PJA2 Gene

Aliases for PJA2 Gene

  • Praja Ring Finger Ubiquitin Ligase 2 2 3 5
  • Praja Ring Finger 2, E3 Ubiquitin Protein Ligase 2 3
  • RING-Type E3 Ubiquitin Transferase Praja-2 3 4
  • Ring Finger Protein 131 2 3
  • Praja2 3 4
  • RNF131 3 4
  • E3 Ubiquitin-Protein Ligase Praja-2 3
  • Praja 2, RING-H2 Motif Containing 3
  • RING Finger Protein 131 4
  • Praja Ring Finger 2 2
  • EC 2.3.2.27 4
  • Neurodap1 3
  • KIAA0438 4

External Ids for PJA2 Gene

Previous HGNC Symbols for PJA2 Gene

  • RNF131

Previous GeneCards Identifiers for PJA2 Gene

  • GC05M108701
  • GC05M108746
  • GC05M108733
  • GC05M108698
  • GC05M103843
  • GC05M108670

Summaries for PJA2 Gene

GeneCards Summary for PJA2 Gene

PJA2 (Praja Ring Finger Ubiquitin Ligase 2) is a Protein Coding gene. Among its related pathways are Class I MHC mediated antigen processing and presentation and Innate Immune System. Gene Ontology (GO) annotations related to this gene include ligase activity and protein kinase A catalytic subunit binding. An important paralog of this gene is PJA1.

UniProtKB/Swiss-Prot for PJA2 Gene

  • Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes.

Gene Wiki entry for PJA2 Gene

Additional gene information for PJA2 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PJA2 Gene

Genomics for PJA2 Gene

GeneHancer (GH) Regulatory Elements for PJA2 Gene

Promoters and enhancers for PJA2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH05J109406 Promoter/Enhancer 2.6 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 663.3 +0.3 307 5.6 FEZF1 DMAP1 YY1 ZNF143 SP3 NFYC ZNF610 GLIS1 NBN NR2C1 PJA2 LOC285638 LINC01023 MAN2A1 GC05P109401
GH05J109454 Enhancer 1.3 FANTOM5 Ensembl ENCODE 8.5 -45.6 -45584 2.6 PKNOX1 ATF1 FEZF1 IRF4 FOS ATF7 RUNX3 SP3 JUNB REST LOC285638 PJA2 ENSG00000248867 GC05P109465 GC05P109466
GH05J109412 Enhancer 1.2 FANTOM5 ENCODE dbSUPER 7.9 -3.5 -3470 1.5 MXI1 MAX BMI1 KMT2B YY1 CC2D1A ZNF766 POLR2A NR2F6 SRF LOC285638 PJA2 GC05P109401 ENSG00000248867
GH05J109328 Enhancer 0.7 Ensembl ENCODE 12.8 +80.2 80249 2.3 JUND CEBPB CEBPG FOS LOC285638 PJA2 GC05P109316
GH05J109643 Enhancer 0.8 ENCODE dbSUPER 5.6 -235.2 -235242 2.8 SCRT1 PKNOX1 NANOG FEZF1 ZIC2 ETS1 POLR2A SCRT2 MAN2A1 PJA2 RN7SKP230 LOC105379118 PIR59063
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PJA2 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the PJA2 gene promoter:
  • C/EBPbeta
  • Sp1
  • YY1
  • AP-1
  • c-Fos
  • c-Jun
  • FosB
  • Fra-1
  • JunB
  • JunD

Genomic Locations for PJA2 Gene

Genomic Locations for PJA2 Gene
chr5:109,334,709-109,409,994
(GRCh38/hg38)
Size:
75,286 bases
Orientation:
Minus strand
chr5:108,670,410-108,745,695
(GRCh37/hg19)
Size:
75,286 bases
Orientation:
Minus strand

Genomic View for PJA2 Gene

Genes around PJA2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PJA2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PJA2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PJA2 Gene

Proteins for PJA2 Gene

  • Protein details for PJA2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43164-PJA2_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase Praja-2
    Protein Accession:
    O43164
    Secondary Accessions:
    • A8K6U4
    • D3DSZ5
    • Q68D49
    • Q8N1G5

    Protein attributes for PJA2 Gene

    Size:
    708 amino acids
    Molecular mass:
    78214 Da
    Quaternary structure:
    • Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase.
    SequenceCaution:
    • Sequence=BAA23710.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Alternative splice isoforms for PJA2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PJA2 Gene

Post-translational modifications for PJA2 Gene

  • Ubiquitination at isoforms=2339, isoforms=2320, and isoforms=2268
  • Modification sites at PhosphoSitePlus

Other Protein References for PJA2 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for PJA2 Gene

Domains & Families for PJA2 Gene

Gene Families for PJA2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for PJA2 Gene

Suggested Antigen Peptide Sequences for PJA2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with PJA2: view

No data available for UniProtKB/Swiss-Prot for PJA2 Gene

Function for PJA2 Gene

Molecular function for PJA2 Gene

UniProtKB/Swiss-Prot Function:
Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes.
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Enzyme Numbers (IUBMB) for PJA2 Gene

Gene Ontology (GO) - Molecular Function for PJA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IDA 21423175
GO:0016740 transferase activity IEA --
GO:0034236 protein kinase A catalytic subunit binding IMP 21423175
GO:0034237 protein kinase A regulatory subunit binding IMP 21423175
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with PJA2: view
genes like me logo Genes that share phenotypes with PJA2: view

Animal Models for PJA2 Gene

MGI Knock Outs for PJA2:
  • Pja2 Pja2<tm1b(EUCOMM)Wtsi>

Animal Model Products

CRISPR Products

miRNA for PJA2 Gene

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PJA2

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PJA2 Gene

Localization for PJA2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PJA2 Gene

Cytoplasm. Cell membrane. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses (By similarity). Colocalizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PJA2 gene
Compartment Confidence
plasma membrane 5
cytoskeleton 4
nucleus 3
endoplasmic reticulum 3
golgi apparatus 3
cytosol 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Intermediate filaments (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PJA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0005737 cytoplasm IDA,IEA 21423175
GO:0005783 endoplasmic reticulum IEA --
GO:0005789 endoplasmic reticulum membrane IEA --
GO:0005794 Golgi apparatus IEA --
genes like me logo Genes that share ontologies with PJA2: view

Pathways & Interactions for PJA2 Gene

genes like me logo Genes that share pathways with PJA2: view

Pathways by source for PJA2 Gene

UniProtKB/Swiss-Prot O43164-PJA2_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for PJA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007616 long-term memory ISS --
GO:0010738 regulation of protein kinase A signaling IMP 21423175
GO:0016567 protein ubiquitination IEA,IDA --
GO:0035329 hippo signaling IEA --
genes like me logo Genes that share ontologies with PJA2: view

No data available for SIGNOR curated interactions for PJA2 Gene

Drugs & Compounds for PJA2 Gene

No Compound Related Data Available

Transcripts for PJA2 Gene

mRNA/cDNA for PJA2 Gene

Unigene Clusters for PJA2 Gene

Praja ring finger 2, E3 ubiquitin protein ligase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PJA2

Alternative Splicing Database (ASD) splice patterns (SP) for PJA2 Gene

ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11
SP1:
SP2: - - -
SP3: -
SP4:

Relevant External Links for PJA2 Gene

GeneLoc Exon Structure for
PJA2
ECgene alternative splicing isoforms for
PJA2

Expression for PJA2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for PJA2 Gene

Protein differential expression in normal tissues from HIPED for PJA2 Gene

This gene is overexpressed in Bone (19.0), Frontal cortex (10.2), Fetal Brain (9.1), Testis (8.5), and Blymphocyte (7.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for PJA2 Gene



NURSA nuclear receptor signaling pathways regulating expression of PJA2 Gene:

PJA2

SOURCE GeneReport for Unigene cluster for PJA2 Gene:

Hs.483036

Evidence on tissue expression from TISSUES for PJA2 Gene

  • Nervous system(4.9)
  • Liver(4.4)
  • Heart(2)
  • Spleen(2)
genes like me logo Genes that share expression patterns with PJA2: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for PJA2 Gene

Orthologs for PJA2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for PJA2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PJA2 34 33
  • 99.39 (n)
OneToOne
dog
(Canis familiaris)
Mammalia PJA2 34 33
  • 89.2 (n)
OneToOne
cow
(Bos Taurus)
Mammalia PJA2 34 33
  • 87.65 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Pja2 16 34 33
  • 85.27 (n)
rat
(Rattus norvegicus)
Mammalia Pja2 33
  • 84.33 (n)
oppossum
(Monodelphis domestica)
Mammalia -- 34
  • 56 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 34 (a)
OneToMany
chicken
(Gallus gallus)
Aves PJA2 33
  • 63.51 (n)
-- 34
  • 48 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 34
  • 49 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia pja2 33
  • 57.54 (n)
Str.4831 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.13459 33
zebrafish
(Danio rerio)
Actinopterygii LOC101882388 33
  • 58.89 (n)
pja2 34
  • 38 (a)
OneToMany
Dr.6114 33
Species where no ortholog for PJA2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for PJA2 Gene

ENSEMBL:
Gene Tree for PJA2 (if available)
TreeFam:
Gene Tree for PJA2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for PJA2: view image

Paralogs for PJA2 Gene

Paralogs for PJA2 Gene

(2) SIMAP similar genes for PJA2 Gene using alignment to 1 proteins:

  • PJA2_HUMAN
genes like me logo Genes that share paralogs with PJA2: view

Variants for PJA2 Gene

Sequence variations from dbSNP and Humsavar for PJA2 Gene

SNP ID Clin Chr 05 pos Variation AA Info Type
rs1000005311 -- 109,335,055(-) A/T 3_prime_UTR_variant
rs1000053205 -- 109,410,943(-) C/A upstream_transcript_variant
rs1000065359 -- 109,335,533(-) CAGT/ 3_prime_UTR_variant
rs1000091196 -- 109,364,170(-) C/A intron_variant
rs1000131894 -- 109,365,829(-) A/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for PJA2 Gene

Variant ID Type Subtype PubMed ID
esv1180527 CNV insertion 17803354
esv1229231 CNV deletion 17803354
esv23192 CNV gain 19812545
esv2730587 CNV deletion 23290073
esv2763481 CNV gain 21179565
esv3303267 CNV mobile element insertion 20981092
esv3378334 CNV insertion 20981092
esv3421599 CNV insertion 20981092
nsv1073479 CNV deletion 25765185
nsv1077993 CNV duplication 25765185
nsv1114715 CNV deletion 24896259
nsv1141499 CNV duplication 24896259
nsv1142001 OTHER inversion 24896259
nsv327359 CNV deletion 16902084
nsv472753 CNV novel sequence insertion 20440878
nsv477073 CNV novel sequence insertion 20440878
nsv599338 CNV gain 21841781
nsv950112 CNV duplication 24416366

Variation tolerance for PJA2 Gene

Residual Variation Intolerance Score: 34.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 10.39; 90.86% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PJA2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PJA2

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PJA2 Gene

Disorders for PJA2 Gene

Additional Disease Information for PJA2

No disorders were found for PJA2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PJA2 Gene

Publications for PJA2 Gene

  1. Control of PKA stability and signalling by the RING ligase praja2. (PMID: 21423175) Lignitto L … Feliciello A (Nature cell biology 2011) 3 4 58
  2. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen JV … Mann M (Cell 2006) 3 4 58
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58
  4. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. (PMID: 12036302) Yu P … Cai T (Genomics 2002) 3 4 58
  5. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro. (PMID: 9455477) Ishikawa K … Ohara O (DNA research : an international journal for rapid publication of reports on genes and genomes 1997) 3 4 58

Products for PJA2 Gene

Sources for PJA2 Gene

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