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Aliases for PIAS4 Gene

Aliases for PIAS4 Gene

  • Protein Inhibitor Of Activated STAT 4 2 3 5
  • Protein Inhibitor Of Activated STAT Protein Gamma 3 4
  • Protein Inhibitor Of Activated STAT Protein 4 3 4
  • RING-Type E3 Ubiquitin Transferase PIAS4 3 4
  • Zinc Finger, MIZ-Type Containing 6 2 3
  • PIAS-Gamma 3 4
  • Protein Inhibitor Of Activated STAT Protein PIASy 3
  • E3 SUMO-Protein Ligase PIAS4 3
  • EC 2.3.2.27 4
  • PIASY 3
  • Piasg 3
  • ZMIZ6 3
  • PIASG 4
  • PIASy 4

External Ids for PIAS4 Gene

Previous GeneCards Identifiers for PIAS4 Gene

  • GC19P003959
  • GC19P003960
  • GC19P003773

Summaries for PIAS4 Gene

GeneCards Summary for PIAS4 Gene

PIAS4 (Protein Inhibitor Of Activated STAT 4) is a Protein Coding gene. Among its related pathways are Cell Cycle, Mitotic and NF-kappa B signaling pathway. Gene Ontology (GO) annotations related to this gene include ligase activity and SUMO transferase activity. An important paralog of this gene is PIAS1.

UniProtKB/Swiss-Prot for PIAS4 Gene

  • Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

Gene Wiki entry for PIAS4 Gene

Additional gene information for PIAS4 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PIAS4 Gene

Genomics for PIAS4 Gene

GeneHancer (GH) Regulatory Elements for PIAS4 Gene

Promoters and enhancers for PIAS4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J004006 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 655.7 +1.5 1482 4.7 ARNT ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 POLR2B CBX5 ZNF207 PIAS4 CACTIN YJU2 ENSG00000267030 HDGFL2 CACTIN-AS1 ENSG00000267769 C19orf71 ATCAY ZFR2
GH19J003363 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 20 -639.9 -639861 8.1 HDGF SMAD1 ARNT YBX1 ZNF2 YY1 POLR2B ZNF213 ZNF143 ZNF263 NFIC CACTIN-AS1 CACTIN YJU2 ZNF77 APBA3 LMNB2 PIAS4 ENSG00000267139 ZNF555
GH19J003965 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 19.2 -38.8 -38766 5.9 HDGF PKNOX1 FOXA2 MLX ARID4B NEUROD1 SIN3A FEZF1 DMAP1 ZBTB7B DAPK3 PIAS4 HDGFL2 CACTIN-AS1 PLIN5 GC19M003970 MIR637
GH19J003605 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE dbSUPER 20.1 -400.0 -399995 4.6 HDGF PKNOX1 ARNT SIN3A YBX1 ZNF2 POLR2B ZNF766 SP3 MCM5 CACTIN-AS1 TBXA2R ZNF77 CACTIN ENSG00000267030 ZNF555 ENSG00000267769 YJU2 APBA3 GNA11
GH19J004246 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 20.4 +239.7 239734 2.2 CLOCK DMAP1 IRF4 YY1 SLC30A9 ZNF213 E2F8 ZNF143 ZNF263 MCM3 YJU2 ENSG00000280247 CACTIN CACTIN-AS1 ENSG00000267030 ENSG00000267769 APBA3 HDGFL2 KDM4B PIAS4
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PIAS4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the PIAS4 gene promoter:
  • TBP
  • STAT5A
  • STAT1beta
  • STAT1alpha
  • STAT1
  • c-Myb
  • GATA-1
  • YY1
  • HNF-4alpha1
  • Nkx3-1

Genomic Locations for PIAS4 Gene

Genomic Locations for PIAS4 Gene
chr19:4,007,598-4,039,386
(GRCh38/hg38)
Size:
31,789 bases
Orientation:
Plus strand
chr19:4,007,644-4,039,384
(GRCh37/hg19)
Size:
31,741 bases
Orientation:
Plus strand

Genomic View for PIAS4 Gene

Genes around PIAS4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PIAS4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PIAS4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PIAS4 Gene

Proteins for PIAS4 Gene

  • Protein details for PIAS4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8N2W9-PIAS4_HUMAN
    Recommended name:
    E3 SUMO-protein ligase PIAS4
    Protein Accession:
    Q8N2W9
    Secondary Accessions:
    • O75926
    • Q96G19
    • Q9UN16

    Protein attributes for PIAS4 Gene

    Size:
    510 amino acids
    Molecular mass:
    56504 Da
    Quaternary structure:
    • Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with MTA1.
    SequenceCaution:
    • Sequence=AAD45155.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for PIAS4 Gene

Post-translational modifications for PIAS4 Gene

  • Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Ubiquitination at isoforms=504, isoforms=231, and Lys114
  • Modification sites at PhosphoSitePlus

Other Protein References for PIAS4 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for PIAS4 Gene

Domains & Families for PIAS4 Gene

Gene Families for PIAS4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for PIAS4 Gene

Suggested Antigen Peptide Sequences for PIAS4 Gene

Graphical View of Domain Structure for InterPro Entry

Q8N2W9

UniProtKB/Swiss-Prot:

PIAS4_HUMAN :
  • The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Belongs to the PIAS family.
Domain:
  • The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
Family:
  • Belongs to the PIAS family.
genes like me logo Genes that share domains with PIAS4: view

Function for PIAS4 Gene

Molecular function for PIAS4 Gene

UniProtKB/Swiss-Prot Function:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Enzyme Numbers (IUBMB) for PIAS4 Gene

Phenotypes From GWAS Catalog for PIAS4 Gene

Gene Ontology (GO) - Molecular Function for PIAS4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003714 transcription corepressor activity IEA --
GO:0005515 protein binding IPI 11388671
GO:0008022 protein C-terminus binding IEA --
GO:0008270 zinc ion binding NAS 9724754
genes like me logo Genes that share ontologies with PIAS4: view
genes like me logo Genes that share phenotypes with PIAS4: view

Animal Models for PIAS4 Gene

MGI Knock Outs for PIAS4:

Animal Model Products

  • Taconic Biosciences Mouse Models for PIAS4

Clone Products

  • Addgene plasmids for PIAS4

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PIAS4 Gene

Localization for PIAS4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PIAS4 Gene

Nucleus, PML body. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PIAS4 gene
Compartment Confidence
nucleus 5
cytosol 2
extracellular 1
peroxisome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for PIAS4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus NAS 9724754
GO:0005654 nucleoplasm TAS,IEA --
GO:0005737 cytoplasm IDA 16816390
GO:0016363 nuclear matrix IEA --
GO:0016605 PML body IEA --
genes like me logo Genes that share ontologies with PIAS4: view

Pathways & Interactions for PIAS4 Gene

genes like me logo Genes that share pathways with PIAS4: view

UniProtKB/Swiss-Prot Q8N2W9-PIAS4_HUMAN

  • Pathway: Protein modification; protein sumoylation.

SIGNOR curated interactions for PIAS4 Gene

Activates:
Inactivates:

Gene Ontology (GO) - Biological Process for PIAS4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II IEA --
GO:0006303 double-strand break repair via nonhomologous end joining TAS --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway IEA --
genes like me logo Genes that share ontologies with PIAS4: view

Drugs & Compounds for PIAS4 Gene

(2) Drugs for PIAS4 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with PIAS4: view

Transcripts for PIAS4 Gene

Unigene Clusters for PIAS4 Gene

Protein inhibitor of activated STAT, 4:
Representative Sequences:

Clone Products

  • Addgene plasmids for PIAS4

Alternative Splicing Database (ASD) splice patterns (SP) for PIAS4 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14
SP1: - - -
SP2:
SP3: - - - -
SP4: - -
SP5: -

Relevant External Links for PIAS4 Gene

GeneLoc Exon Structure for
PIAS4
ECgene alternative splicing isoforms for
PIAS4

Expression for PIAS4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for PIAS4 Gene

Protein differential expression in normal tissues from HIPED for PIAS4 Gene

This gene is overexpressed in Fetal Brain (18.4), Urinary Bladder (15.1), Retina (8.6), and Liver (8.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for PIAS4 Gene



Protein tissue co-expression partners for PIAS4 Gene

NURSA nuclear receptor signaling pathways regulating expression of PIAS4 Gene:

PIAS4

SOURCE GeneReport for Unigene cluster for PIAS4 Gene:

Hs.105779

mRNA Expression by UniProt/SwissProt for PIAS4 Gene:

Q8N2W9-PIAS4_HUMAN
Tissue specificity: Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

Evidence on tissue expression from TISSUES for PIAS4 Gene

  • Nervous system(4.5)
  • Skin(4.2)
  • Lung(4.1)
genes like me logo Genes that share expression patterns with PIAS4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for PIAS4 Gene

Orthologs for PIAS4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PIAS4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PIAS4 34
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia PIAS4 34
  • 91 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PIAS4 34 33
  • 89.28 (n)
OneToOne
dog
(Canis familiaris)
Mammalia PIAS4 34 33
  • 88.69 (n)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 88 (a)
OneToMany
-- 34
  • 84 (a)
OneToMany
mouse
(Mus musculus)
Mammalia Pias4 16 34 33
  • 83.53 (n)
rat
(Rattus norvegicus)
Mammalia Pias4 33
  • 83.27 (n)
chicken
(Gallus gallus)
Aves PIAS4 34 33
  • 82.33 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PIAS4 34
  • 73 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia pias4 33
  • 69.78 (n)
Str.11012 33
African clawed frog
(Xenopus laevis)
Amphibia LOC398703 33
zebrafish
(Danio rerio)
Actinopterygii pias4a 34 33
  • 63.15 (n)
OneToMany
pias4b 34
  • 41 (a)
OneToMany
wufi20e09 33
fruit fly
(Drosophila melanogaster)
Insecta Su(var)2-10 34
  • 31 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea gei-17 34
  • 17 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NFI1 36 34
  • 12 (a)
SIZ1 34
  • 9 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 24 (a)
OneToMany
Species where no ortholog for PIAS4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PIAS4 Gene

ENSEMBL:
Gene Tree for PIAS4 (if available)
TreeFam:
Gene Tree for PIAS4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for PIAS4: view image

Paralogs for PIAS4 Gene

Paralogs for PIAS4 Gene

(5) SIMAP similar genes for PIAS4 Gene using alignment to 2 proteins:

  • PIAS4_HUMAN
  • Q05DS6_HUMAN
genes like me logo Genes that share paralogs with PIAS4: view

Variants for PIAS4 Gene

Sequence variations from dbSNP and Humsavar for PIAS4 Gene

SNP ID Clin Chr 19 pos Variation AA Info Type
rs1000030136 -- 4,026,924(+) T/C intron_variant
rs1000048417 -- 4,037,851(+) G/T coding_sequence_variant, missense_variant
rs1000209809 -- 4,030,126(+) C/T intron_variant
rs1000214899 -- 4,009,707(+) T/C/G genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant
rs1000284377 -- 4,033,790(+) C/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for PIAS4 Gene

Variant ID Type Subtype PubMed ID
dgv495e201 CNV deletion 23290073
esv2658425 CNV deletion 23128226
esv2662737 CNV deletion 23128226
esv2670138 CNV deletion 23128226
esv2671087 CNV deletion 23128226
esv2717979 CNV deletion 23290073
esv2717980 CNV deletion 23290073
esv2717981 CNV deletion 23290073
esv2717982 CNV deletion 23290073
esv2717983 CNV deletion 23290073
esv2717986 CNV deletion 23290073
esv2717988 CNV deletion 23290073
esv3555892 CNV deletion 23714750
esv3643486 CNV gain 21293372
esv9150 CNV loss 19470904
nsv1120352 CNV tandem duplication 24896259
nsv1146574 CNV insertion 26484159
nsv470115 CNV loss 18288195
nsv476927 CNV novel sequence insertion 20440878
nsv480690 CNV novel sequence insertion 20440878
nsv519665 CNV loss 19592680
nsv578435 CNV loss 21841781
nsv828401 CNV gain 20364138
nsv828402 CNV gain 20364138
nsv953949 CNV deletion 24416366

Variation tolerance for PIAS4 Gene

Residual Variation Intolerance Score: 13.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.25; 25.01% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PIAS4 Gene

Human Gene Mutation Database (HGMD)
PIAS4
SNPedia medical, phenotypic, and genealogical associations of SNPs for
PIAS4

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PIAS4 Gene

Disorders for PIAS4 Gene

Additional Disease Information for PIAS4

No disorders were found for PIAS4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PIAS4 Gene

Publications for PIAS4 Gene

  1. SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. (PMID: 15831457) Ihara M … Kikuchi A (Molecular and cellular biology 2005) 3 4 22 58
  2. Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner. (PMID: 12631292) Dahle Ø … Gabrielsen OS (European journal of biochemistry 2003) 3 4 22 58
  3. Sumoylation is involved in beta-catenin-dependent activation of Tcf-4. (PMID: 12727872) Yamamoto H … Kikuchi A (The EMBO journal 2003) 3 4 22 58
  4. Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy. (PMID: 12750312) Chun TH … Nakao K (Circulation research 2003) 3 4 22 58
  5. A transcriptional corepressor of Stat1 with an essential LXXLL signature motif. (PMID: 11248056) Liu B … Shuai K (Proceedings of the National Academy of Sciences of the United States of America 2001) 3 4 22 58

Products for PIAS4 Gene

Sources for PIAS4 Gene

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