PGA4 Gene (Protein Coding)

Pepsinogen A4

GC11P061222
GIFtS:
30
This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromos... See more...

Aliases for PGA4 Gene

Aliases for PGA4 Gene

  • Pepsinogen A4 2 3 5
  • Pepsinogen 4, Group I (Pepsinogen A) 2 3
  • Pepsinogen-4 3 4
  • Pepsin A-4 3 4
  • Pregnancy-Associated Glycoprotein 3
  • EC 3.4.23.1 4
  • Pepsin A 2
  • PGA4 5

External Ids for PGA4 Gene

Previous GeneCards Identifiers for PGA4 Gene

  • GC11U990101
  • GC11P060765
  • GC11P060727
  • GC11P060747
  • GC11P060739
  • GC11P060989

Summaries for PGA4 Gene

Entrez Gene Summary for PGA4 Gene

  • This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

GeneCards Summary for PGA4 Gene

PGA4 (Pepsinogen A4) is a Protein Coding gene. Diseases associated with PGA4 include Atrophic Gastritis and Gastritis. Among its related pathways are Surfactant metabolism and Collagen chain trimerization. Gene Ontology (GO) annotations related to this gene include aspartic-type endopeptidase activity. An important paralog of this gene is PGA3.

UniProtKB/Swiss-Prot Summary for PGA4 Gene

  • Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Additional gene information for PGA4 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PGA4 Gene

Genomics for PGA4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Download GeneHancer 2017 data
Promoters and enhancers for PGA4 Gene
GeneHancer (GH) Identifier GH Type GH
Score		 GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites		 Gene Targets
GH11J061221 Promoter/Enhancer 0.7 EPDnew Ensembl dbSUPER 250.7 -0.1 -147 3.2 HSALNG0084505-001 PGA4 PGA5 piR-45035-035 PGA3 HSALNG0084505-002
GH11J061240 Promoter/Enhancer 0.7 EPDnew Ensembl 0.4 +18.8 18754 3 EZH2 ZNF512 PRDM1 HSALNG0084505-002 PGA5 HSALNG0084506 piR-45035-036 ENSG00000256220 PGA4
GH11J061217 Enhancer 0.3 Ensembl dbSUPER 0.7 -4.2 -4213 1.7 HSALNG0084505-001 PGA4 piR-45035-035 PGA3 HSALNG0084505-002
GH11J061227 Enhancer 0.3 Ensembl dbSUPER 0.4 +5.1 5054 4.8 ENSG00000256220 piR-45035-036 PGA4 HSALNG0084505-002 PGA5 HSALNG0084506
GH11J061212 Enhancer 0.3 Ensembl dbSUPER 0.4 -8.0 -8046 2.2 piR-45035-035 HSALNG0084505-001 PGA4 PGA3 HSALNG0084505-002
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PGA4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PGA4

Top Transcription factor binding sites by QIAGEN in the PGA4 gene promoter:
  • FOXD1

Genomic Locations for PGA4 Gene

Latest Assembly
chr11:61,222,347-61,231,694
(GRCh38/hg38)
Size:
9,348 bases
Orientation:
Plus strand

Previous Assembly
chr11:60,989,819-60,999,166
(GRCh37/hg19 by Entrez Gene)
Size:
9,348 bases
Orientation:
Plus strand

chr11:60,989,688-61,018,929
(GRCh37/hg19 by Ensembl)
Size:
29,242 bases
Orientation:
Plus strand

Genomic View for PGA4 Gene

Genes around PGA4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PGA4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PGA4 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for PGA4 Gene

Proteins for PGA4 Gene

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  1. Antibodies for research
  2. Protein products for research
  3. Assay products for research

  • Protein details for PGA4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P0DJD7-PEPA4_HUMAN
    Recommended name:
    Pepsin A-4
    Protein Accession:
    P0DJD7
    Secondary Accessions:
    • A8K749
    • B7ZW75
    • P00790
    • Q7M4R0
    • Q8N1E3

    Protein attributes for PGA4 Gene

    Size:
    388 amino acids
    Molecular mass:
    41977 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for PGA4 Gene

neXtProt entry for PGA4 Gene

Post-translational modifications for PGA4 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for PGA4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibodies for research

  • Boster Bio Antibodies for PGA4

No data available for DME Specific Peptides for PGA4 Gene

Domains & Families for PGA4 Gene

Gene Families for PGA4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins
  • Predicted secreted proteins

Protein Domains for PGA4 Gene

InterPro:

Suggested Antigen Peptide Sequences for PGA4 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ58952, highly similar to Pepsin A (EC 3.4.23.1) (B7Z719_HUMAN)
  • Pepsinogen 4, group I (Pepsinogen A) (B7ZW10_HUMAN)
  • Pepsinogen 4, group I (Pepsinogen A) (B7ZW16_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P0DJD7

UniProtKB/Swiss-Prot:

PEPA4_HUMAN :
  • Belongs to the peptidase A1 family.
Family:
  • Belongs to the peptidase A1 family.
genes like me logo Genes that share domains with PGA4: view

Function for PGA4 Gene

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  3. miRNA products for research
  4. Inhibitory RNAs for research
  5. Clone products for research
  6. Cell Lines for research

Molecular function for PGA4 Gene

UniProtKB/Swiss-Prot Function:
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.; EC=3.4.23.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10094};.
GENATLAS Biochemistry:
pepsinogen 4,group I (pepsinogen A),clustered with PGA3,PGA5

Enzyme Numbers (IUBMB) for PGA4 Gene

Gene Ontology (GO) - Molecular Function for PGA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004190 aspartic-type endopeptidase activity IEA --
GO:0005515 protein binding IPI 32296183
GO:0008233 peptidase activity IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with PGA4: view
genes like me logo Genes that share phenotypes with PGA4: view

Animal Models for research

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PGA4

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for PGA4 Gene

Localization for PGA4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PGA4 Gene

Secreted.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PGA4 gene
Compartment Confidence
extracellular 5
endosome 4
mitochondrion 1

Gene Ontology (GO) - Cellular Components for PGA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region IEA --
GO:0070062 extracellular exosome HDA 23533145
GO:0097486 multivesicular body lumen TAS --
genes like me logo Genes that share ontologies with PGA4: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for PGA4 Gene

Pathways & Interactions for PGA4 Gene

genes like me logo Genes that share pathways with PGA4: view

Pathways by source for PGA4 Gene

2 Reactome pathways for PGA4 Gene
1 KEGG pathway for PGA4 Gene

Gene Ontology (GO) - Biological Process for PGA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0007586 digestion IEA --
GO:0044267 cellular protein metabolic process TAS --
genes like me logo Genes that share ontologies with PGA4: view

No data available for SIGNOR curated interactions for PGA4 Gene

Drugs & Compounds for PGA4 Gene

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  1. Drug products for research
No Compound Related Data Available

Transcripts for PGA4 Gene

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  1. CRISPR products for research
  2. miRNA products for research
  3. Inhibitory RNAs for research
  4. Clone products for research

mRNA/cDNA for PGA4 Gene

1 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PGA4

Alternative Splicing Database (ASD) splice patterns (SP) for PGA4 Gene

No ASD Table

Relevant External Links for PGA4 Gene

GeneLoc Exon Structure for
PGA4

Expression for PGA4 Gene

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  1. Primer products for research
  2. Gene Expression Assays for research

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PGA4 Gene

mRNA expression in normal human tissues for PGA4 Gene
mRNA expression by GTEx for PGA4 GenemRNA expression by BioGPS for PGA4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PGA4 Gene

This gene is overexpressed in Stomach (x52.6).

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for PGA4

SOURCE GeneReport for Unigene cluster for PGA4 Gene:

Hs.661883

Evidence on tissue expression from TISSUES for PGA4 Gene

  • Stomach(4.9)
  • Intestine(4.5)
  • Kidney(4.2)
  • Muscle(4.2)
  • Nervous system(2.3)
genes like me logo Genes that share expression patterns with PGA4: view

Primer products for research

No data available for Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for PGA4 Gene

Orthologs for PGA4 Gene

This gene was present in the common ancestor of chordates.

Orthologs for PGA4 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
 Mammalia PGA4 29
  • 99.14 (n)
-- 30
  • 99 (a)
 OneToMany
Dog
(Canis familiaris)
 Mammalia PGA 29 30
  • 86.1 (n)
 OneToMany
Cow
(Bos Taurus)
 Mammalia PGA5 29
  • 84.72 (n)
PGA 30
  • 82 (a)
 OneToMany
Mouse
(Mus musculus)
 Mammalia Cym 30
  • 54 (a)
 ManyToMany
Oppossum
(Monodelphis domestica)
 Mammalia -- 30
  • 41 (a)
 ManyToMany
Chicken
(Gallus gallus)
 Aves LOC395926 29
  • 63.53 (n)
PGA 30
  • 62 (a)
 OneToMany
Lizard
(Anolis carolinensis)
 Reptilia -- 30
  • 72 (a)
 OneToMany
Tropical Clawed Frog
(Silurana tropicalis)
 Amphibia pga4 29
  • 68.14 (n)
Species where no ortholog for PGA4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rat (Rattus norvegicus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)
  • Zebrafish (Danio rerio)

Evolution for PGA4 Gene

ENSEMBL:
Gene Tree for PGA4 (if available)
TreeFam:
Gene Tree for PGA4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for PGA4: view image
Alliance of Genome Resources:
Additional Orthologs for PGA4

Paralogs for PGA4 Gene

Paralogs for PGA4 Gene

(8) SIMAP similar genes for PGA4 Gene using alignment to 3 proteins:

  • PEPA4_HUMAN
  • B7ZW10_HUMAN
  • B7ZW16_HUMAN
genes like me logo Genes that share paralogs with PGA4: view

Variants for PGA4 Gene

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  1. SNP Genotyping and Copy Number Assays for research

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for PGA4 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for PGA4 Gene

Variant ID Type Subtype PubMed ID
dgv1960n54 CNV loss 21841781
dgv1961n54 CNV loss 21841781
esv1004439 CNV deletion 20482838
esv2744587 CNV deletion 23290073
esv2759831 CNV gain+loss 17122850
esv2761679 CNV loss 21179565
esv29298 CNV gain+loss 19812545
esv3350259 CNV duplication 20981092
esv3382959 CNV duplication 20981092
esv3425930 CNV duplication 20981092
esv3579734 CNV loss 25503493
nsv1115412 CNV duplication 24896259
nsv1118213 CNV deletion 24896259
nsv339 CNV deletion 18451855
nsv340 CNV insertion 18451855
nsv428259 CNV gain+loss 18775914
nsv509414 CNV insertion 20534489
nsv514629 CNV gain 21397061
nsv555162 CNV loss 21841781
nsv555163 CNV loss 21841781
nsv820586 CNV deletion 20802225
nsv825937 CNV loss 20364138
nsv832176 CNV gain+loss 17160897
nsv832177 CNV loss 17160897
nsv8833 CNV gain 18304495
nsv952458 CNV duplication 24416366
nsv975192 CNV duplication 23825009

Variation tolerance for PGA4 Gene

Gene Damage Index Score: 0.06; 1.46% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PGA4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PGA4
Leiden Open Variation Database (LOVD)
PGA4

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for PGA4 Gene

Disorders for PGA4 Gene

MalaCards: The human disease database

(6) MalaCards diseases for PGA4 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
atrophic gastritis
  • gastric atrophy
gastritis
  • acute gastric mucosal erosion
chief cell adenoma
  • chief cell adenoma of parathyroid gland
macular degeneration, age-related, 10
  • armd10
gastric cancer
  • gastric cancer, somatic
- elite association - COSMIC cancer census association via MalaCards
Search PGA4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for PGA4

Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with PGA4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PGA4 Gene

Publications for PGA4 Gene

  1. Primary structure of human pepsinogen gene. (PMID: 6300126) Sogawa K … Takahashi K (The Journal of biological chemistry 1983) 2 3 4
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  3. Crystal structure of human pepsin and its complex with pepstatin. (PMID: 7663352) Fujinaga M … James MN (Protein science : a publication of the Protein Society 1995) 3 4
  4. A comparative study on the NH2-terminal amino acid sequences and some other properties of six isozymic forms of human pepsinogens and pepsins. (PMID: 2515193) Athauda SB … Takahashi K (Journal of biochemistry 1989) 3 4
  5. Nucleotide sequence comparison of five human pepsinogen A (PGA) genes: evolution of the PGA multigene family. (PMID: 2714789) Evers MP … Eriksson AW (Genomics 1989) 3 4

ExternalLinks

View latest publications for PGA4 gene in Mastermind

Products for PGA4 Gene

Sources for PGA4 Gene