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Aliases for PAK4 Gene

Aliases for PAK4 Gene

  • P21 (RAC1) Activated Kinase 4 2 3 5
  • P21 Protein (Cdc42/Rac)-Activated Kinase 4 2 3
  • Serine/Threonine-Protein Kinase PAK 4 3 4
  • P21(CDKN1A)-Activated Kinase 4 2 3
  • EC 2.7.11.1 4 56
  • Protein Kinase Related To S. Cerevisiae STE20, Effector For Cdc42Hs 3
  • P21-Activated Kinase 4 4
  • EC 2.7.11 56
  • KIAA1142 4
  • PAK-4 4

External Ids for PAK4 Gene

Previous GeneCards Identifiers for PAK4 Gene

  • GC19P040228
  • GC19P040007
  • GC19P044292
  • GC19P044308
  • GC19P044274
  • GC19P039616
  • GC19P036064

Summaries for PAK4 Gene

Entrez Gene Summary for PAK4 Gene

  • PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for PAK4 Gene

PAK4 (P21 (RAC1) Activated Kinase 4) is a Protein Coding gene. Diseases associated with PAK4 include Breast Adenoma. Among its related pathways are Immune response IL-23 signaling pathway and Human cytomegalovirus infection. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PAK5.

UniProtKB/Swiss-Prot for PAK4 Gene

  • Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Gene Wiki entry for PAK4 Gene

Additional gene information for PAK4 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PAK4 Gene

Genomics for PAK4 Gene

GeneHancer (GH) Regulatory Elements for PAK4 Gene

Promoters and enhancers for PAK4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J039124 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 630.8 +1.1 1124 5.4 ZNF652 SP1 ZFX CC2D1A ZNF148 NKRF POLR2A ZNF121 CEBPG GTF2F1 PAK4 PIR35823 HNRNPL FBL ZNF780B ZNF780A ENSG00000269688 ENSG00000269172 EID2B RN7SL566P
GH19J039397 Promoter/Enhancer 2.8 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 51.6 +281.3 281305 20 HDGF ZNF473 MTA3 SP1 ZNF652 CC2D1A ZFX ELF3 CTCF ZKSCAN8 PAF1 ZFP36 HNRNPL ZNF780B FBL AKT2 SUPT5H ZNF780A RN7SL566P RPS16
GH19J038647 Promoter/Enhancer 2.6 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 50.6 -466.0 -465978 25.6 ZNF473 SP1 ZFX ELF3 ZKSCAN8 MNT CBFA2T2 ZNF148 NKRF POLR2A ACTN4 HNRNPL ZFP30 ZNF607 ZNF781 ZNF573 SUPT5H ENSG00000267152 PAK4 RN7SL566P
GH19J039389 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 33.5 +265.2 265182 3.7 ZFX SP1 SIX5 NKRF POLR2A ZNF121 CEBPG GTF2F1 CSDE1 ZNF687 MED29 PAF1 HNRNPL EID2B PAK4 ZNF780B FBL SUPT5H AKT2 PSMC4
GH19J039029 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 33.1 -93.7 -93727 4.4 HDGF SP1 ZFX NKRF ZNF121 CEBPG ZNF687 ZSCAN21 AHR TFE3 FBXO27 ENSG00000267992 HNRNPL RN7SL566P FBL PAK4 PLEKHG2 ENSG00000269688 PAF1 SUPT5H
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PAK4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the PAK4 gene promoter:
  • AREB6
  • CBF(2)
  • E47
  • Hand1
  • NF-kappaB
  • NF-kappaB1
  • NF-kappaB2
  • NF-Y

Genomic Locations for PAK4 Gene

Genomic Locations for PAK4 Gene
chr19:39,125,770-39,182,816
(GRCh38/hg38)
Size:
57,047 bases
Orientation:
Plus strand
chr19:39,616,410-39,673,456
(GRCh37/hg19)
Size:
57,047 bases
Orientation:
Plus strand

Genomic View for PAK4 Gene

Genes around PAK4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PAK4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PAK4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PAK4 Gene

Proteins for PAK4 Gene

  • Protein details for PAK4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O96013-PAK4_HUMAN
    Recommended name:
    Serine/threonine-protein kinase PAK 4
    Protein Accession:
    O96013
    Secondary Accessions:
    • B4DGG6
    • Q8N4E1
    • Q8NCH5
    • Q8NDE3
    • Q9BU33
    • Q9ULS8

    Protein attributes for PAK4 Gene

    Size:
    591 amino acids
    Molecular mass:
    64072 Da
    Quaternary structure:
    • Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1 (PubMed:15827085). Interacts with INKA1 (PubMed:26607847). Interacts with SH3RF2 (PubMed:24130170).
    SequenceCaution:
    • Sequence=BAA86456.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAC11166.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PAK4 Gene

    Alternative splice isoforms for PAK4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PAK4 Gene

Selected DME Specific Peptides for PAK4 Gene

O96013:
  • GPEVDIWS
  • LEGGALTDIVTH
  • EVDIWSLG
  • PSNFEHRVHTGFD
  • DFGFCAQ
  • MDLRKQQRRELLFNEVVIMRDY
  • HEQFRAALQ
  • KIGEGSTG
  • GTPYWMAPE
  • QGVIHRDIKSDSILL
  • SYLVGDELWVVME

Post-translational modifications for PAK4 Gene

  • Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.31). Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
  • Polyubiquitinated, leading to its proteasomal degradation.
  • Ubiquitination at posLast=3131
  • Modification sites at PhosphoSitePlus

Domains & Families for PAK4 Gene

Gene Families for PAK4 Gene

IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for PAK4 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ55049, highly similar to Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (B4DE88_HUMAN)
  • cDNA FLJ61413, highly similar to Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (B4DUG0_HUMAN)
  • p21-activated kinase 4 (PAK4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O96013

UniProtKB/Swiss-Prot:

PAK4_HUMAN :
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Family:
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
genes like me logo Genes that share domains with PAK4: view

Function for PAK4 Gene

Molecular function for PAK4 Gene

UniProtKB/Swiss-Prot Function:
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by INKA1; which inhibits the serine/threonine-protein kinase activity by binding PAK4 in a substrate-like manner (PubMed:26607847).

Enzyme Numbers (IUBMB) for PAK4 Gene

Phenotypes From GWAS Catalog for PAK4 Gene

Gene Ontology (GO) - Molecular Function for PAK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity NAS 9822598
GO:0004674 protein serine/threonine kinase activity IMP 21788589
GO:0005515 protein binding IPI 20936779
GO:0005524 ATP binding IEA --
GO:0016301 kinase activity IEA --
genes like me logo Genes that share ontologies with PAK4: view
genes like me logo Genes that share phenotypes with PAK4: view

Animal Models for PAK4 Gene

MGI Knock Outs for PAK4:
  • Pak4 Pak4<tm1Amin>
  • Pak4 Pak4<tm2.2Amin>

Animal Model Products

  • Taconic Biosciences Mouse Models for PAK4

CRISPR Products

miRNA for PAK4 Gene

miRTarBase miRNAs that target PAK4

Clone Products

  • Applied Biological Materials (abm): Clones for PAK4 - Now 50% OFF >
  • * PAK4 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
  • * PAK4 tags and reporters available: His, HA, Myc, Flag, GFP, RFP, Luciferase - Browse All
  • Addgene plasmids for PAK4

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PAK4 Gene

Localization for PAK4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PAK4 Gene

Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways. {ECO:0000269 PubMed:12356872}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PAK4 gene
Compartment Confidence
golgi apparatus 4
cytosol 3
cytoskeleton 2
nucleus 2
plasma membrane 1
extracellular 1
peroxisome 1
endoplasmic reticulum 1
mitochondrion 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cell Junctions (3)
  • Plasma membrane (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PAK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IBA 21873635
GO:0005794 Golgi apparatus TAS 9822598
GO:0005913 cell-cell adherens junction HDA 25468996
GO:0005925 focal adhesion HDA 21423176
genes like me logo Genes that share ontologies with PAK4: view

Pathways & Interactions for PAK4 Gene

genes like me logo Genes that share pathways with PAK4: view

SIGNOR curated interactions for PAK4 Gene

Activates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for PAK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001558 regulation of cell growth TAS 20070256
GO:0006468 protein phosphorylation IEA --
GO:0006915 apoptotic process TAS 20070256
GO:0007010 cytoskeleton organization TAS 20070256
GO:0007049 cell cycle IEA --
genes like me logo Genes that share ontologies with PAK4: view

Drugs & Compounds for PAK4 Gene

(23) Drugs for PAK4 Gene - From: DrugBank, ClinicalTrials, ApexBio, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Nicotinamide Approved, Investigational Pharma 505,505
Niacin Approved, Investigational Nutra Unknown, Agonist, Full agonist 509,509
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
Folic Acid Approved, Vet_approved Nutra 4566
Guanosine triphosphate Experimental Pharma 0

(1) Additional Compounds for PAK4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Full agonist, Agonist, Partial agonist, Antagonist, Gating inhibitor 58-64-0

(2) ApexBio Compounds for PAK4 Gene

Compound Action Cas Number
KPT-9274 Orally acitve allosteric inhibitor of PAK4 1643913-93-2
PF-3758309 PAK4 inhibitor 898044-15-0
genes like me logo Genes that share compounds with PAK4: view

Transcripts for PAK4 Gene

Unigene Clusters for PAK4 Gene

P21 protein (Cdc42/Rac)-activated kinase 4:
Representative Sequences:

CRISPR Products

Clone Products

  • Applied Biological Materials (abm): Clones for PAK4 - Now 50% OFF >
  • * PAK4 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
  • * PAK4 tags and reporters available: His, HA, Myc, Flag, GFP, RFP, Luciferase - Browse All
  • Addgene plasmids for PAK4

Alternative Splicing Database (ASD) splice patterns (SP) for PAK4 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b
SP1: -
SP2: - -
SP3: - -
SP4: - - -
SP5: - - -
SP6: - - - -
SP7: - - - - - -
SP8: - - - - - - -
SP9: - - - -
SP10: - - - - - - - - -
SP11: - - - - -
SP12: - - - - - - - -
SP13:
SP14:

Relevant External Links for PAK4 Gene

GeneLoc Exon Structure for
PAK4
ECgene alternative splicing isoforms for
PAK4

Expression for PAK4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for PAK4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for PAK4 Gene

This gene is overexpressed in Breast (52.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for PAK4 Gene



Protein tissue co-expression partners for PAK4 Gene

NURSA nuclear receptor signaling pathways regulating expression of PAK4 Gene:

PAK4

SOURCE GeneReport for Unigene cluster for PAK4 Gene:

Hs.20447

mRNA Expression by UniProt/SwissProt for PAK4 Gene:

O96013-PAK4_HUMAN
Tissue specificity: Highest expression in prostate, testis and colon.

Evidence on tissue expression from TISSUES for PAK4 Gene

  • Nervous system(4.8)
  • Pancreas(4.7)
  • Liver(4.3)
  • Eye(4.2)
  • Adrenal gland(2.1)
  • Lung(2.1)
genes like me logo Genes that share expression patterns with PAK4: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for PAK4 Gene

Orthologs for PAK4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PAK4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PAK4 35
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PAK4 35 34
  • 91.4 (n)
OneToOne
cow
(Bos Taurus)
Mammalia PAK4 35 34
  • 91 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pak4 34
  • 86.89 (n)
mouse
(Mus musculus)
Mammalia Pak4 17 35 34
  • 86.67 (n)
oppossum
(Monodelphis domestica)
Mammalia PAK4 35
  • 82 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 42 (a)
ManyToMany
chicken
(Gallus gallus)
Aves PAK4 35 34
  • 76.62 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PAK4 35
  • 65 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia pak4 34
  • 65.98 (n)
Str.17438 34
African clawed frog
(Xenopus laevis)
Amphibia PAK5 34
zebrafish
(Danio rerio)
Actinopterygii pak4 35 34
  • 67.43 (n)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta mbt 35 36 34
  • 66.44 (n)
OneToMany
Pak3 36
  • 48 (a)
worm
(Caenorhabditis elegans)
Secernentea C45B11.1a 36
  • 48 (a)
C45B11.1b 36
  • 48 (a)
pak-2 35
  • 47 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes STE20 35
  • 21 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10829 35
  • 42 (a)
OneToMany
Species where no ortholog for PAK4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PAK4 Gene

ENSEMBL:
Gene Tree for PAK4 (if available)
TreeFam:
Gene Tree for PAK4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for PAK4: view image

Paralogs for PAK4 Gene