This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011] See more...

Aliases for OTUB2 Gene

Aliases for OTUB2 Gene

  • OTU Deubiquitinase, Ubiquitin Aldehyde Binding 2 2 3 5
  • OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 2 3 4
  • Ubiquitin-Specific-Processing Protease OTUB2 3 4
  • OTU Domain, Ubiquitin Aldehyde Binding 2 2 3
  • Deubiquitinating Enzyme OTUB2 3 4
  • Ubiquitin Thioesterase OTUB2 3 4
  • C14orf137 3 4
  • Otubain-2 3 4
  • OTB2 3 4
  • OTU2 3 4
  • Ubiquitin-Specific Protease Otubain 2 3
  • Chromosome 14 Open Reading Frame 137 2
  • EC 3.4.19.12 4
  • FLJ21916 2
  • MGC3102 2
  • OTUB2 5

External Ids for OTUB2 Gene

Previous HGNC Symbols for OTUB2 Gene

  • C14orf137

Previous GeneCards Identifiers for OTUB2 Gene

  • GC14P092483
  • GC14P093562
  • GC14P094492
  • GC14P074672

Summaries for OTUB2 Gene

Entrez Gene Summary for OTUB2 Gene

  • This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]

GeneCards Summary for OTUB2 Gene

OTUB2 (OTU Deubiquitinase, Ubiquitin Aldehyde Binding 2) is a Protein Coding gene. Among its related pathways are Deubiquitination and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include hydrolase activity and omega peptidase activity. An important paralog of this gene is OTUB1.

UniProtKB/Swiss-Prot Summary for OTUB2 Gene

  • Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Gene Wiki entry for OTUB2 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for OTUB2 Gene

Genomics for OTUB2 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for OTUB2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH14J094025 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 256.7 +1.6 1602 4.3 ZBTB10 SP1 MNT MBD2 ZNF217 ZBTB7B ZIC2 HOMEZ ZNF610 THAP11 OTUB2 HSALNG0103342 BTBD7 DDX24 CCDC197 ASB2 IFI27L2 IFI27L1 IFI27 lnc-IFI27L2-3
GH14J093935 Enhancer 0.8 Ensembl dbSUPER 10.6 -89.7 -89739 1.2 CTCF SIN3A FOXA2 RAD21 SMC3 ZNF24 ZNF547 MIXL1 ZNF184 ZNF143 ASB2 CCDC197 OTUB2 HSALNG0103331 FAM181A-AS1 FAM181A
GH14J094088 Enhancer 0.5 Ensembl 10.6 +63.1 63061 0.8 RELA FOSL1 CAVIN1 NR2C1 PTTG1 ID3 NR4A1 MEF2D ZNF592 IFI27L2 OTUB2 IFI27L1 ENSG00000241499 HSALNG0103345 NONHSAG015770.2 RF00994-345 IFI27
GH14J094097 Enhancer 0.4 Ensembl ENCODE 10.3 +72.2 72169 1 ZFHX2 HSALNG0103345 HSALNG0103346 IFI27L1 IFI27L2 OTUB2 IFI27 RF00994-345 NONHSAG015770.2
GH14J093322 Enhancer 0.8 ENCODE dbSUPER 5.1 -702.6 -702561 2.1 ZNF316 NFE2 RAD51 EP400 ZNF362 MAFG MAFF ATF2 HDAC1 MEIS2 COX8C UNC79 OTUB2 UBR7 BTBD7 HSALNG0103309 HSALNG0103310 HE855966
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around OTUB2 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for OTUB2

Top Transcription factor binding sites by QIAGEN in the OTUB2 gene promoter:
  • ATF
  • ATF-2
  • c-Jun
  • HNF-1
  • Lhx3a
  • LHX3b
  • p53
  • POU2F1
  • POU2F1a
  • POU6F1 (c2)

Genomic Locations for OTUB2 Gene

Latest Assembly
chr14:94,026,340-94,048,930
(GRCh38/hg38)
Size:
22,591 bases
Orientation:
Plus strand

Previous Assembly
chr14:94,492,686-94,515,276
(GRCh37/hg19 by Entrez Gene)
Size:
22,591 bases
Orientation:
Plus strand

chr14:94,492,675-94,515,276
(GRCh37/hg19 by Ensembl)
Size:
22,602 bases
Orientation:
Plus strand

Genomic View for OTUB2 Gene

Genes around OTUB2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
OTUB2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for OTUB2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for OTUB2 Gene

Proteins for OTUB2 Gene

  • Protein details for OTUB2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96DC9-OTUB2_HUMAN
    Recommended name:
    Ubiquitin thioesterase OTUB2
    Protein Accession:
    Q96DC9
    Secondary Accessions:
    • Q6IA10
    • Q9H6T1

    Protein attributes for OTUB2 Gene

    Size:
    234 amino acids
    Molecular mass:
    27213 Da
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • In the structure described by PubMed:15258613, the Asp-48 active site of the catalytic triad is located too far to interact directly with the active site His-224. A possible explanation is that OTUB2 is in inactive conformation in absence of ubiquitin and a conformation change may move Asp-48 in the proximity of His-224 in presence of ubiquitin substrate.

    Three dimensional structures from OCA and Proteopedia for OTUB2 Gene

    Alternative splice isoforms for OTUB2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for OTUB2 Gene

Post-translational modifications for OTUB2 Gene

  • Ubiquitination at Lys94
  • Modification sites at PhosphoSitePlus

Other Protein References for OTUB2 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for OTUB2 Gene

Domains & Families for OTUB2 Gene

Gene Families for OTUB2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for OTUB2 Gene

GenScript: Design optimal peptide antigens:
  • Ubiquitin-specific-processing protease OTUB2 (OTUB2_HUMAN)
  • OTUB2 protein (Q9BWI9_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q96DC9

UniProtKB/Swiss-Prot:

OTUB2_HUMAN :
  • Belongs to the peptidase C65 family.
Family:
  • Belongs to the peptidase C65 family.
genes like me logo Genes that share domains with OTUB2: view

Function for OTUB2 Gene

Molecular function for OTUB2 Gene

UniProtKB/Swiss-Prot Function:
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:23827681};.

Enzyme Numbers (IUBMB) for OTUB2 Gene

Phenotypes From GWAS Catalog for OTUB2 Gene

Gene Ontology (GO) - Molecular Function for OTUB2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004843 thiol-dependent ubiquitin-specific protease activity IBA,IMP 15258613
GO:0005515 protein binding IPI 25416956
GO:0008233 peptidase activity IEA --
GO:0008234 cysteine-type peptidase activity IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with OTUB2: view
genes like me logo Genes that share phenotypes with OTUB2: view

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for OTUB2

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for OTUB2 Gene

Localization for OTUB2 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for OTUB2 gene
Compartment Confidence
nucleus 4
cytoskeleton 3
cytosol 3
plasma membrane 1
extracellular 1
mitochondrion 1
endoplasmic reticulum 1
endosome 1
lysosome 1
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for OTUB2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA 21873635
genes like me logo Genes that share ontologies with OTUB2: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Subcellular locations from the Human Protein Atlas (HPA) for OTUB2 Gene

Pathways & Interactions for OTUB2 Gene

genes like me logo Genes that share pathways with OTUB2: view

Pathways by source for OTUB2 Gene

Gene Ontology (GO) - Biological Process for OTUB2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0016579 protein deubiquitination IMP 15258613
GO:0018215 protein phosphopantetheinylation IEA --
GO:0035871 protein K11-linked deubiquitination IBA,IDA 23827681
GO:0070536 protein K63-linked deubiquitination IBA,IDA 18954305
genes like me logo Genes that share ontologies with OTUB2: view

No data available for SIGNOR curated interactions for OTUB2 Gene

Drugs & Compounds for OTUB2 Gene

No Compound Related Data Available

Transcripts for OTUB2 Gene

mRNA/cDNA for OTUB2 Gene

1 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for OTUB2

Alternative Splicing Database (ASD) splice patterns (SP) for OTUB2 Gene

No ASD Table

Relevant External Links for OTUB2 Gene

GeneLoc Exon Structure for
OTUB2

Expression for OTUB2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for OTUB2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

  • Testis (Reproductive System)

mRNA differential expression in normal tissues according to GTEx for OTUB2 Gene

This gene is overexpressed in Testis (x10.3), Skin - Sun Exposed (Lower leg) (x6.9), and Skin - Not Sun Exposed (Suprapubic) (x5.5).

Protein differential expression in normal tissues from HIPED for OTUB2 Gene

This gene is overexpressed in Ovary (34.6) and Testis (32.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for OTUB2 Gene



Protein tissue co-expression partners for OTUB2 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for OTUB2

SOURCE GeneReport for Unigene cluster for OTUB2 Gene:

Hs.278815

mRNA Expression by UniProt/SwissProt for OTUB2 Gene:

Q96DC9-OTUB2_HUMAN
Tissue specificity: Widely expressed. Expressed at higher level in brain.

Evidence on tissue expression from TISSUES for OTUB2 Gene

  • Lung(4.1)
  • Eye(4)
genes like me logo Genes that share expression patterns with OTUB2: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for OTUB2 Gene

Orthologs for OTUB2 Gene

This gene was present in the common ancestor of animals.

Orthologs for OTUB2 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia OTUB2 30 31
  • 99.57 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia OTUB2 30 31
  • 92.99 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Otub2 30 17 31
  • 91.7 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia OTUB2 30 31
  • 91.31 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Otub2 30
  • 90.46 (n)
Oppossum
(Monodelphis domestica)
Mammalia OTUB2 31
  • 89 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia OTUB2 31
  • 79 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia otub2 30
  • 59.73 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta CG4968 31
  • 32 (a)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea otub-1 31
  • 22 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.5787 31
  • 31 (a)
OneToMany
Species where no ortholog for OTUB2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Zebrafish (Danio rerio)

Evolution for OTUB2 Gene

ENSEMBL:
Gene Tree for OTUB2 (if available)
TreeFam:
Gene Tree for OTUB2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for OTUB2: view image

Paralogs for OTUB2 Gene

Paralogs for OTUB2 Gene

(2) SIMAP similar genes for OTUB2 Gene using alignment to 3 proteins:

  • OTUB2_HUMAN
  • Q8WYS4_HUMAN
  • Q9BWI9_HUMAN
genes like me logo Genes that share paralogs with OTUB2: view

Variants for OTUB2 Gene

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for OTUB2 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for OTUB2 Gene

Variant ID Type Subtype PubMed ID
esv34067 CNV loss 18971310
esv3581391 CNV loss 25503493
esv3635373 CNV gain 21293372
nsv565620 CNV loss 21841781
nsv952233 CNV deletion 24416366

Variation tolerance for OTUB2 Gene

Residual Variation Intolerance Score: 68% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.69; 46.15% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for OTUB2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
OTUB2

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for OTUB2 Gene

Disorders for OTUB2 Gene

Additional Disease Information for OTUB2

No disorders were found for OTUB2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for OTUB2 Gene

Publications for OTUB2 Gene

  1. Otubains: a new family of cysteine proteases in the ubiquitin pathway. (PMID: 12704427) Balakirev MY … Chroboczek J (EMBO reports 2003) 2 3 4 23
  2. Crystal structure of human otubain 2. (PMID: 15258613) Nanao MH … Balakirev MY (EMBO reports 2004) 3 4 23
  3. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. (PMID: 23827681) Mevissen TE … Komander D (Cell 2013) 3 4
  4. Regulation of virus-triggered signaling by OTUB1- and OTUB2-mediated deubiquitination of TRAF3 and TRAF6. (PMID: 19996094) Li S … Shu HB (The Journal of biological chemistry 2010) 2 3
  5. Structural basis and specificity of human otubain 1-mediated deubiquitination. (PMID: 18954305) Edelmann MJ … Kessler BM (The Biochemical journal 2009) 3 4

Products for OTUB2 Gene

Sources for OTUB2 Gene