This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011] See more...

Aliases for NUCB2 Gene

Aliases for NUCB2 Gene

  • Nucleobindin 2 2 3 5
  • Novel DNA Binding/EF-Hand/Leucine Zipper Protein 2 3
  • Epididymis Secretory Protein Li 109 3 4
  • Gastric Cancer Antigen Zg4 3 4
  • DNA-Binding Protein NEFA 3 4
  • Nucleobindin-2 3 4
  • Prepronesfatin 3 4
  • NEFA 3 4
  • Nucleobinding 2 3
  • Nesfatin 1 3
  • HEL-S-109 3

External Ids for NUCB2 Gene

Previous GeneCards Identifiers for NUCB2 Gene

  • GC11M018347
  • GC11P018224
  • GC11P017321
  • GC11P017262
  • GC11P017255
  • GC11P016982
  • GC11P017229
  • GC11P017212

Summaries for NUCB2 Gene

Entrez Gene Summary for NUCB2 Gene

  • This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

GeneCards Summary for NUCB2 Gene

NUCB2 (Nucleobindin 2) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include calcium ion binding. An important paralog of this gene is NUCB1.

UniProtKB/Swiss-Prot Summary for NUCB2 Gene

  • Calcium-binding protein. May have a role in calcium homeostasis.
  • Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).

Gene Wiki entry for NUCB2 Gene

Additional gene information for NUCB2 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NUCB2 Gene

Genomics for NUCB2 Gene

GeneHancer (GH) Regulatory Elements for NUCB2 Gene

Promoters and enhancers for NUCB2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH11J017206 Promoter/Enhancer 2.4 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas 750.6 +0.3 279 4.9 ZBTB40 SREBF1 ZBTB6 MLX SMARCE1 POLR2A TCF7L2 CTBP1 USF2 ATF1 PIK3C2A NUCB2 lnc-NCR3LG1-4 lnc-NUCB2-4 lnc-PIK3C2A-1 SNORD14A OR7E14P SAAL1 SNORD14B RPS13
GH11J017273 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 758.7 +68.8 68823 6.5 E2F8 SIN3A ZBTB40 SP1 ZBTB6 RBPJ POLR2A FOS CC2D1A NFYC NUCB2 lnc-NCR3LG1-2 SAAL1 OR7E14P SNORD14A lnc-KCNJ11-5
GH11J017295 Promoter 0.5 EPDnew 750.2 +87.2 87163 0.1 FOXA2 FOXA3 NUCB2 lnc-KCNJ11-5 lnc-NCR3LG1-2
GH11J017263 Enhancer 0.7 Ensembl 4.3 +56.7 56748 2.6 MAFK PRDM1 FOSL2 CTBP1 ZEB2 ZNF629 CEBPB JUN ZNF316 JUND OR7E14P RPS13 KCNJ11 ENSG00000260196 NUCB2 lnc-NCR3LG1-2 RF00017-876
GH11J017221 Enhancer 0.4 Ensembl 5 +14.2 14197 1.7 ZBTB40 ZNF384 JUN OR7E14P RPS13 SNORD14B NCR3LG1 NUCB2 RPS2P40 piR-50444-057
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NUCB2 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the NUCB2 gene promoter:
  • AML1a
  • HNF-4alpha1
  • HNF-4alpha2
  • NF-kappaB
  • NF-kappaB1
  • Pax-5
  • PPAR-alpha
  • SREBP-1a
  • SREBP-1b
  • SREBP-1c

Genomic Locations for NUCB2 Gene

Genomic Locations for NUCB2 Gene
chr11:17,208,153-17,349,980
(GRCh38/hg38)
Size:
141,828 bases
Orientation:
Plus strand
chr11:17,229,700-17,371,521
(GRCh37/hg19)
Size:
141,822 bases
Orientation:
Plus strand

Genomic View for NUCB2 Gene

Genes around NUCB2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NUCB2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NUCB2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NUCB2 Gene

Proteins for NUCB2 Gene

  • Protein details for NUCB2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P80303-NUCB2_HUMAN
    Recommended name:
    Nucleobindin-2
    Protein Accession:
    P80303
    Secondary Accessions:
    • A8K642
    • D3DQX5
    • Q8NFT5
    • V9HW75

    Protein attributes for NUCB2 Gene

    Size:
    420 amino acids
    Molecular mass:
    50223 Da
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

    Alternative splice isoforms for NUCB2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NUCB2 Gene

Post-translational modifications for NUCB2 Gene

  • Glycosylation at Ser42, Ser96, Thr163, Thr172, Thr314, Thr322, Tyr389, and Ser408
  • Ubiquitination at Lys90 and Lys247
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for NUCB2 Gene

Domains & Families for NUCB2 Gene

Gene Families for NUCB2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Predicted secreted proteins

Protein Domains for NUCB2 Gene

Suggested Antigen Peptide Sequences for NUCB2 Gene

GenScript: Design optimal peptide antigens:
  • Nucleobindin 2 (A7LNF3_HUMAN)
  • Nucleobindin 2 (A7LNF4_HUMAN)
  • Nucleobindin 2 (A7LNF5_HUMAN)
  • Nucleobinding 2 (D3DQX5_HUMAN)
  • Gastric cancer antigen Zg4 (NUCB2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P80303

UniProtKB/Swiss-Prot:

NUCB2_HUMAN :
  • Belongs to the nucleobindin family.
Family:
  • Belongs to the nucleobindin family.
genes like me logo Genes that share domains with NUCB2: view

Function for NUCB2 Gene

Molecular function for NUCB2 Gene

UniProtKB/Swiss-Prot Function:
Calcium-binding protein. May have a role in calcium homeostasis.
UniProtKB/Swiss-Prot Function:
Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).

Phenotypes From GWAS Catalog for NUCB2 Gene

Gene Ontology (GO) - Molecular Function for NUCB2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0005509 calcium ion binding IEA,IBA 21873635
GO:0005515 protein binding IPI 21988832
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with NUCB2: view
genes like me logo Genes that share phenotypes with NUCB2: view

Animal Model Products

  • Taconic Biosciences Mouse Models for NUCB2

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NUCB2

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NUCB2 Gene

Localization for NUCB2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NUCB2 Gene

Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm. Secreted. Endoplasmic reticulum. Nucleus envelope. Note=Golgi retention is mediated by its N-terminal region.
Nesfatin-1: Secreted.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NUCB2 gene
Compartment Confidence
extracellular 5
plasma membrane 4
endoplasmic reticulum 4
cytosol 4
golgi apparatus 4
nucleus 3
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NUCB2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region IEA --
GO:0005615 extracellular space TAS,HDA 16502470
GO:0005634 nucleus IEA --
GO:0005635 nuclear envelope IEA --
GO:0005640 nuclear outer membrane IEA --
genes like me logo Genes that share ontologies with NUCB2: view

Pathways & Interactions for NUCB2 Gene

PathCards logo

SuperPathways for NUCB2 Gene

No Data Available

Gene Ontology (GO) - Biological Process for NUCB2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0032099 negative regulation of appetite IEA --
genes like me logo Genes that share ontologies with NUCB2: view

No data available for Pathways by source and SIGNOR curated interactions for NUCB2 Gene

Drugs & Compounds for NUCB2 Gene

(4) Drugs for NUCB2 Gene - From: DrugBank and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Calcium Citrate Approved, Investigational Pharma Target, ligand 0
Calcium Phosphate Approved Pharma Target, ligand 0
Calcium phosphate dihydrate Approved Pharma Target 0
Calcium Approved Nutra 7773
genes like me logo Genes that share compounds with NUCB2: view

Transcripts for NUCB2 Gene

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NUCB2

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NUCB2 Gene

No ASD Table

Relevant External Links for NUCB2 Gene

GeneLoc Exon Structure for
NUCB2
ECgene alternative splicing isoforms for
NUCB2

Expression for NUCB2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NUCB2 Gene

Protein differential expression in normal tissues from HIPED for NUCB2 Gene

This gene is overexpressed in Skin (23.8) and Salivary gland (12.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NUCB2 Gene



Protein tissue co-expression partners for NUCB2 Gene

NURSA nuclear receptor signaling pathways regulating expression of NUCB2 Gene:

NUCB2

SOURCE GeneReport for Unigene cluster for NUCB2 Gene:

Hs.654599

mRNA Expression by UniProt/SwissProt for NUCB2 Gene:

P80303-NUCB2_HUMAN
Tissue specificity: Predominantly expressed in spleen, testis and normal stomach.

Evidence on tissue expression from TISSUES for NUCB2 Gene

  • Liver(4.5)
  • Blood(4.4)
  • Pancreas(3)
  • Nervous system(3)
  • Stomach(2.8)
  • Bone marrow(2.4)
  • Intestine(2.3)
  • Heart(2.2)
  • Adrenal gland(2.1)
  • Lung(2)
  • Thyroid gland(2)
genes like me logo Genes that share expression patterns with NUCB2: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NUCB2 Gene

Orthologs for NUCB2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NUCB2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NUCB2 33 32
  • 99.84 (n)
OneToOne
dog
(Canis familiaris)
Mammalia NUCB2 33 32
  • 93.24 (n)
OneToOne
cow
(Bos Taurus)
Mammalia NUCB2 33 32
  • 91.73 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NUCB2 33
  • 86 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nucb2 32
  • 83.85 (n)
mouse
(Mus musculus)
Mammalia Nucb2 17 33 32
  • 83.77 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia NUCB2 33
  • 79 (a)
OneToOne
chicken
(Gallus gallus)
Aves NUCB2 33 32
  • 79.82 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NUCB2 33
  • 72 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nucb2 32
  • 68.16 (n)
zebrafish
(Danio rerio)
Actinopterygii nucb2a 33 32
  • 68.26 (n)
OneToMany
nucb2b 33
  • 63 (a)
OneToMany
Dr.85 32
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.7060 32
fruit fly
(Drosophila melanogaster)
Insecta NUCB1 33 32
  • 50.92 (n)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005608 32
  • 50.65 (n)
worm
(Caenorhabditis elegans)
Secernentea nucb-1 33 32
  • 53.69 (n)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6711 33
  • 36 (a)
OneToMany
Cin.12858 32
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.12858 32
Species where no ortholog for NUCB2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NUCB2 Gene

ENSEMBL:
Gene Tree for NUCB2 (if available)
TreeFam:
Gene Tree for NUCB2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NUCB2: view image

Paralogs for NUCB2 Gene

Paralogs for NUCB2 Gene

(2) SIMAP similar genes for NUCB2 Gene using alignment to 14 proteins:

  • NUCB2_HUMAN
  • A7LNF3_HUMAN
  • A7LNF4_HUMAN
  • A7LNF5_HUMAN
  • E9PJP3_HUMAN
  • E9PKG6_HUMAN
  • E9PLE9_HUMAN
  • E9PLR0_HUMAN
  • E9PLR5_HUMAN
  • E9PM22_HUMAN
  • E9PRQ3_HUMAN
  • H0YD18_HUMAN
  • H0YEG8_HUMAN
  • J3KQU0_HUMAN
genes like me logo Genes that share paralogs with NUCB2: view

Variants for NUCB2 Gene

Polymorphic Variants from UniProtKB/Swiss-Prot for NUCB2 Gene

NUCB2_HUMAN-P80303
Deletion of Gln-402 is frequent.

Sequence variations from dbSNP and Humsavar for NUCB2 Gene

SNP ID Clin Chr 11 pos Variation AA Info Type
rs214088 likely-pathogenic, not provided 17,276,578(+) C/G/T genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant
rs182903196 not-provided, not provided 17,276,676(+) G/A genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant
rs186174 not-provided, not provided 17,276,557(+) A/C/G genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant
rs214087 not-provided, not provided 17,276,818(+) G/C 5_prime_UTR_variant, genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant
rs4757506 not-provided, not provided 17,276,730(+) A/G/T 5_prime_UTR_variant, genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for NUCB2 Gene

Variant ID Type Subtype PubMed ID
esv3625473 CNV loss 21293372
esv3625474 CNV loss 21293372
nsv467711 CNV gain 19166990
nsv469762 CNV gain 16826518
nsv524611 CNV loss 19592680
nsv553567 CNV gain 21841781
nsv553568 CNV gain 21841781
nsv553582 CNV loss 21841781
nsv7692 CNV insertion 18451855

Variation tolerance for NUCB2 Gene

Residual Variation Intolerance Score: 45.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 13.14; 95.14% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NUCB2 Gene

Human Gene Mutation Database (HGMD)
NUCB2
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NUCB2

SNP Genotyping and Copy Number Assay Products

Disorders for NUCB2 Gene

Additional Disease Information for NUCB2

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for NUCB2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NUCB2 Gene

Publications for NUCB2 Gene

  1. Troglitazone, a ligand of peroxisome proliferator-activated receptor-{gamma}, stabilizes NUCB2 (Nesfatin) mRNA by activating the ERK1/2 pathway: isolation and characterization of the human NUCB2 gene. (PMID: 20427483) Yamada M … Mori M (Endocrinology 2010) 3 4 23 56
  2. Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. (PMID: 7811391) Barnikol-Watanabe S … Hilschmann N (Biological chemistry Hoppe-Seyler 1994) 2 3 4 56
  3. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PMID: 20628086) Bailey SD … DREAM investigators (Diabetes care 2010) 3 43 56
  4. Nucleobindin-2/nesfatin in the endocrine pancreas: distribution and relationship to glycaemic state. (PMID: 20032201) Foo KS … Broberger C (The Journal of endocrinology 2010) 3 23 56
  5. [Influence of AML1 and AML1-ETO on transcriptional regulation of nucb2 gene]. (PMID: 20030931) Zou B … Wang KK (Zhongguo shi yan xue ye xue za zhi 2009) 3 23 56

Products for NUCB2 Gene

  • Boster Bio Antibodies for NUCB2
  • Boster Bio ELISA Kits for NUCB2

Sources for NUCB2 Gene