The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for t... See more...

Aliases for NT5E Gene

Aliases for NT5E Gene

  • 5'-Nucleotidase Ecto 2 3 5
  • Ecto-5'-Nucleotidase 3 4
  • 5'-Nucleotidase 3 4
  • EC 3.1.3.5 4 51
  • CALJA 2 3
  • 5'-NT 3 4
  • CD73 2 3
  • NT5 3 4
  • NTE 3 4
  • ENT 2 3
  • EN 2 3
  • 5'-Nucleotidase, Ecto (CD73) 2
  • Purine 5-Prime-Nucleotidase 3
  • 5' Nucleotidase (CD73) 2
  • CD73 Antigen 4
  • E5NT 3
  • NT5E 5
  • NT 3

External Ids for NT5E Gene

Previous HGNC Symbols for NT5E Gene

  • NT5

Previous GeneCards Identifiers for NT5E Gene

  • GC06P086106
  • GC06P086155
  • GC06P086216
  • GC06P083383

Summaries for NT5E Gene

Entrez Gene Summary for NT5E Gene

  • The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

CIViC Summary for NT5E Gene

GeneCards Summary for NT5E Gene

NT5E (5'-Nucleotidase Ecto) is a Protein Coding gene. Diseases associated with NT5E include Calcification Of Joints And Arteries and Intestinal Tuberculosis. Among its related pathways are Neuroscience and ATP/ITP metabolism. Gene Ontology (GO) annotations related to this gene include nucleotide binding and hydrolase activity, acting on ester bonds.

UniProtKB/Swiss-Prot Summary for NT5E Gene

  • Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene Wiki entry for NT5E Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NT5E Gene

Genomics for NT5E Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for NT5E Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NT5E on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NT5E

Top Transcription factor binding sites by QIAGEN in the NT5E gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • GR
  • GR-alpha
  • GR-beta
  • SREBP-1a
  • SREBP-1b
  • SREBP-1c

Genomic Locations for NT5E Gene

Genomic Locations for NT5E Gene
chr6:85,449,584-85,495,791
(GRCh38/hg38)
Size:
46,208 bases
Orientation:
Plus strand
chr6:86,159,302-86,205,509
(GRCh37/hg19)
Size:
46,208 bases
Orientation:
Plus strand

Genomic View for NT5E Gene

Genes around NT5E on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NT5E Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NT5E Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NT5E Gene

Proteins for NT5E Gene

  • Protein details for NT5E Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P21589-5NTD_HUMAN
    Recommended name:
    5'-nucleotidase
    Protein Accession:
    P21589
    Secondary Accessions:
    • B3KQI8
    • O75520
    • Q5W116

    Protein attributes for NT5E Gene

    Size:
    574 amino acids
    Molecular mass:
    63368 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for NT5E Gene

    Alternative splice isoforms for NT5E Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NT5E Gene

Selected DME Specific Peptides for NT5E Gene

P21589:
  • YDAMALGNHEFDNGVEGLI
  • RECNMGNLICDAMI
  • LTILHTND
  • VGYTSKETP
  • YPAVEGRI
  • TGEFLQV
  • YISKMKV
  • GKYLGYLK
  • GNPILLNS
  • GGDGFQMIKDE

Post-translational modifications for NT5E Gene

  • Glycosylation at Asn53, Asn311, Asn333, and Asn403
  • Modification sites at PhosphoSitePlus

Other Protein References for NT5E Gene

Antibody Products

  • Abcam antibodies for NT5E

Domains & Families for NT5E Gene

Gene Families for NT5E Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • CD markers
  • Disease related genes
  • Enzymes
  • Potential drug targets
  • Predicted intracellular proteins
  • Predicted membrane proteins

Protein Domains for NT5E Gene

Suggested Antigen Peptide Sequences for NT5E Gene

GenScript: Design optimal peptide antigens:
  • Ecto-5'-nucleotidase (5NTD_HUMAN)
  • cDNA FLJ90527 fis, clone NT2RP4001467, highly similar to 5'-nucleotidase (EC 3.1.3.5) (B3KQI8_HUMAN)
  • Ecto-5'-nucleotidase (Q53Z63_HUMAN)
  • 5'-nucleotidase, ecto (CD73) (Q5JRQ1_HUMAN)
  • 5'-nucleotidase, ecto (CD73) (Q5JRQ2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P21589

UniProtKB/Swiss-Prot:

5NTD_HUMAN :
  • Belongs to the 5'-nucleotidase family.
Family:
  • Belongs to the 5'-nucleotidase family.
genes like me logo Genes that share domains with NT5E: view

Function for NT5E Gene

Molecular function for NT5E Gene

UniProtKB/Swiss-Prot Function:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;.

Enzyme Numbers (IUBMB) for NT5E Gene

Phenotypes From GWAS Catalog for NT5E Gene

Gene Ontology (GO) - Molecular Function for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0008253 5'-nucleotidase activity IDA 7595232
GO:0016787 hydrolase activity IEA --
GO:0016788 hydrolase activity, acting on ester bonds IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with NT5E: view
genes like me logo Genes that share phenotypes with NT5E: view

Human Phenotype Ontology for NT5E Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for NT5E Gene

MGI Knock Outs for NT5E:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NT5E

No data available for Transcription Factor Targets and HOMER Transcription for NT5E Gene

Localization for NT5E Gene

Subcellular locations from UniProtKB/Swiss-Prot for NT5E Gene

Cell membrane. Lipid-anchor, GPI-anchor.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NT5E gene
Compartment Confidence
plasma membrane 5
extracellular 5
nucleus 5
cytosol 5
cytoskeleton 3
mitochondrion 2
endoplasmic reticulum 2
endosome 2
lysosome 2
golgi apparatus 2
peroxisome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Plasma membrane (3)
  • Cytosol (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm IDA --
GO:0005829 cytosol IDA --
GO:0005886 plasma membrane TAS --
GO:0009986 cell surface HDA 19581412
GO:0016020 membrane TAS 2129526
genes like me logo Genes that share ontologies with NT5E: view

Pathways & Interactions for NT5E Gene

genes like me logo Genes that share pathways with NT5E: view

Pathways by source for NT5E Gene

SIGNOR curated interactions for NT5E Gene

Is activated by:

Gene Ontology (GO) - Biological Process for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006195 purine nucleotide catabolic process TAS --
GO:0006196 AMP catabolic process IBA 21873635
GO:0006259 DNA metabolic process TAS 2129526
GO:0007159 leukocyte cell-cell adhesion IDA 7595232
GO:0009166 nucleotide catabolic process IEA --
genes like me logo Genes that share ontologies with NT5E: view

Drugs & Compounds for NT5E Gene

(31) Drugs for NT5E Gene - From: DrugBank, ClinicalTrials, DGIdb, IUPHAR, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cytarabine Approved, Experimental, Investigational Pharma Enzyme, substrate 1335
Pentoxifylline Approved, Investigational Pharma Target, inhibitor 124
Etidronic acid Approved Pharma 37
Adenosine Approved, Investigational Pharma Agonist, Channel blocker 619
Phosphoric acid Approved Pharma 0

(23) Additional Compounds for NT5E Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
beta-nicotinamide D-ribonucleotide
deoxyadenosine
  • (2R,3S,5R)-5-(6-amino-9H-Purin-9-yl)-2-(hydroxymethyl)tetrahydrofuran-3-ol
  • 5-(6-amino-PURIN-9-yl)-2-hydroxymethyl-tetrahydro-furan-3-ol
  • 9-(2-Deoxy-beta-D-erythro-pentofuranosyl)adenine
  • 9-(2-Deoxy-beta-D-ribofuranosyl)-9H-purin-6-amine
  • Adenine deoxyribonucleoside
958-09-8
Deoxyadenosine monophosphate
  • 2'-dAMP
  • 2'-Deoxy-5'-adenosine monophosphate
  • 2'-Deoxy-AMP
  • 2'-Deoxyadenosine 5'-(dihydrogen phosphate)
  • 2'-Deoxyadenosine 5'-monophosphate
653-63-4
deoxyguanosine
  • 2-amino-9-(2-Deoxy-9-beta-D-ribofuranosyl)-9H-purin-6-ol
  • 9-(2-Deoxy-beta-D-erythro-pentofuranosyl)-guanine
  • dG
  • Guanine deoxy nucleoside
  • 2-amino-9-(2-Deoxy-9-b-D-ribofuranosyl)-9H-purin-6-ol
961-07-9
nicotinate beta-D-ribonucleotide
  • beta-Nicotinate D-ribonucleotide
  • Nicotinate ribonucleotide
  • Nicotinic acid ribonucleotide
  • b-Nicotinate D-ribonucleotide
  • b-Nicotinic acid D-ribonucleotide
genes like me logo Genes that share compounds with NT5E: view

Transcripts for NT5E Gene

mRNA/cDNA for NT5E Gene

2 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NT5E

Alternative Splicing Database (ASD) splice patterns (SP) for NT5E Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
SP1: -
SP2: - -
SP3:

Relevant External Links for NT5E Gene

GeneLoc Exon Structure for
NT5E

Expression for NT5E Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NT5E Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NT5E Gene

This gene is overexpressed in Ovary (10.1) and Bone marrow mesenchymal stem cell (9.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NT5E Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NT5E

SOURCE GeneReport for Unigene cluster for NT5E Gene:

Hs.153952

Evidence on tissue expression from TISSUES for NT5E Gene

  • Blood(4.7)
  • Liver(4.6)
  • Nervous system(4.4)
  • Skin(4)
  • Lung(3.8)
  • Bone marrow(3.6)
  • Kidney(3.5)
  • Heart(3.3)
  • Bone(3.2)
  • Spleen(3.2)
  • Muscle(3)
  • Lymph node(3)
  • Intestine(2.9)
  • Eye(2.9)
  • Pancreas(2.7)
  • Stomach(2.2)
  • Thyroid gland(2)

Phenotype-based relationships between genes and organs from Gene ORGANizer for NT5E Gene

Germ Layers:
  • mesoderm
Systems:
  • cardiovascular
  • skeleton
Regions:
General:
  • blood vessel
  • spinal column
  • vertebrae
genes like me logo Genes that share expression patterns with NT5E: view

No data available for mRNA differential expression in normal tissues , Protein tissue co-expression partners and mRNA Expression by UniProt/SwissProt for NT5E Gene

Orthologs for NT5E Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NT5E Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia NT5E 30 31
  • 99.71 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia NT5E 30 31
  • 90.64 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia -- 31
  • 90 (a)
OneToMany
-- 31
  • 89 (a)
OneToMany
Rat
(Rattus norvegicus)
Mammalia Nt5e 30
  • 85.48 (n)
Mouse
(Mus musculus)
Mammalia Nt5e 30 17 31
  • 85.42 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia NT5E 31
  • 74 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia NT5E 31
  • 73 (a)
OneToOne
Chicken
(Gallus gallus)
Aves NT5E 30 31
  • 65.75 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 62 (a)
OneToMany
-- 31
  • 55 (a)
OneToMany
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia nt5e 30
  • 65.42 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.14455 30
Zebrafish
(Danio rerio)
Actinopterygii nt5e 30 31
  • 64.17 (n)
OneToOne
zgc63784 30
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003629 30
  • 49.97 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta veil 30 31
  • 48.99 (n)
OneToMany
CG30104 32
  • 39 (a)
CG4827 32
  • 39 (a)
NT5E-2 31
  • 38 (a)
OneToMany
CG30103 31 32
  • 36 (a)
OneToMany
CG1961 32
  • 33 (a)
CG42249 31
  • 29 (a)
OneToMany
CG11883 31
  • 17 (a)
OneToMany
Bread mold
(Neurospora crassa)
Ascomycetes NCU09659 30
  • 48.35 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 13 (a)
OneToOne
Species where no ortholog for NT5E was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for NT5E Gene

ENSEMBL:
Gene Tree for NT5E (if available)
TreeFam:
Gene Tree for NT5E (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NT5E: view image

Paralogs for NT5E Gene

No data available for Paralogs for NT5E Gene

Variants for NT5E Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NT5E Gene

SNP ID Clinical significance and condition Chr 06 pos Variation AA Info Type
717329 Benign: not provided 85,467,248(+) C/T SYNONYMOUS_VARIANT
727283 Likely Benign: not provided 85,450,215(+) G/T MISSENSE_VARIANT
729150 Benign: not provided 85,493,867(+) A/G MISSENSE_VARIANT
735452 Likely Benign: not provided 85,492,155(+) T/C SYNONYMOUS_VARIANT
738423 Likely Benign: not provided 85,489,596(+) A/C MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for NT5E Gene

Structural Variations from Database of Genomic Variants (DGV) for NT5E Gene

Variant ID Type Subtype PubMed ID
nsv463939 CNV loss 19166990
nsv470839 CNV loss 18288195
nsv520112 CNV loss 19592680
nsv604092 CNV loss 21841781
nsv604093 CNV loss 21841781

Variation tolerance for NT5E Gene

Residual Variation Intolerance Score: 76.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.63; 65.53% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NT5E Gene

Human Gene Mutation Database (HGMD)
NT5E
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NT5E

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NT5E Gene

Disorders for NT5E Gene

MalaCards: The human disease database

(17) MalaCards diseases for NT5E Gene - From: UniProtKB/Swiss-Prot, OMIM, ClinVar, GTR, Orphanet, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
calcification of joints and arteries
  • calja
intestinal tuberculosis
  • tuberculosis of intestine
calciphylaxis
  • idiopathic calciphylaxis
colorectal cancer 5
  • colorectal cancer, susceptibility to, 5
arterial calcification of infancy
  • gaci
- elite association - COSMIC cancer census association via MalaCards
Search NT5E in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

5NTD_HUMAN
  • Calcification of joints and arteries (CALJA) [MIM:211800]: A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. {ECO:0000269 PubMed:21288095, ECO:0000269 PubMed:24887587}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Additional Disease Information for NT5E

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with NT5E: view

No data available for Genatlas for NT5E Gene

Publications for NT5E Gene

  1. Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene. (PMID: 8566797) Hansen KR … Thompson LF (Gene 1995) 3 4 23
  2. NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. (PMID: 24887587) Fausther M … Dranoff JA (PloS one 2014) 3 4
  3. Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling. (PMID: 23142347) Knapp K … Sträter N (Structure (London, England : 1993) 2012) 3 4
  4. The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism. (PMID: 21933152) Garavaglia S … Rizzi M (The Biochemical journal 2012) 3 4
  5. NT5E mutations and arterial calcifications. (PMID: 21288095) St Hilaire C … Boehm M (The New England journal of medicine 2011) 3 4

Products for NT5E Gene

  • Addgene plasmids for NT5E