This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression... See more...

Aliases for NPFF Gene

Aliases for NPFF Gene

  • Neuropeptide FF-Amide Peptide Precursor 2 3 5
  • Pro-FMRFamide-Related Neuropeptide FF 3 4
  • FMRFAL 2 3
  • FMRFamide-Related Peptides 4
  • NPFF 5

External Ids for NPFF Gene

Previous GeneCards Identifiers for NPFF Gene

  • GC12M053896
  • GC12M054012
  • GC12M053618
  • GC12M052186
  • GC12M050940
  • GC12M053507
  • GC12M053509

Summaries for NPFF Gene

Entrez Gene Summary for NPFF Gene

  • This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

GeneCards Summary for NPFF Gene

NPFF (Neuropeptide FF-Amide Peptide Precursor) is a Protein Coding gene. Diseases associated with NPFF include Pain Agnosia and Agnosia. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and neuropeptide hormone activity.

UniProtKB/Swiss-Prot Summary for NPFF Gene

  • Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels.

Gene Wiki entry for NPFF Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NPFF Gene

Genomics for NPFF Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for NPFF Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH12J053507 Promoter/Enhancer 1.3 EPDnew Ensembl ENCODE 250.7 -0.7 -706 1.9 MYC ZNF654 ZIC2 CTCF REST DEK TRIM22 SIN3A ZNF513 THAP11 NPFF lnc-NPFF-1 lnc-TARBP2-1 ATP5MC2 piR-58797 ATF7 ATF7-NPFF MN298678-017
GH12J053504 Enhancer 0.7 Ensembl ENCODE dbSUPER 250.7 +1.8 1813 3 POLR2A ZFHX2 NPFF PRR13 HSALNG0091286 MAP3K12 TARBP2 lnc-NPFF-1 ATF7-NPFF MN298678-017
GH12J053294 Promoter/Enhancer 2.4 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 21.2 +209.7 209725 7 BCLAF1 ZNF207 MYC NCOR1 IKZF1 ZNF600 SSRP1 ZNF580 POLR2A ZNF10 MYG1 PFDN5 piR-50035 piR-46958 ESPL1 AAAS ZNF740 TARBP2 MYG1-AS1 NPFF
GH12J053452 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 23.4 +54.3 54338 4.2 BCLAF1 SP1 ZNF654 ZNF207 ZNF600 NCOR1 MYC IKZF1 SIX5 JUND PCBP2 AAAS ZNF740 SP1 ENSG00000258344 MYG1 MAP3K12 HNRNPA1 TARBP2 NPFF
GH12J053179 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 23.6 +326.0 325982 4.9 NCOR1 MYC ZNF600 SIX5 ZNF580 ZNF592 POLR2A NFIC ZIC2 ZBTB10 ZNF740 CSAD HSALNG0091246 HSALNG0091247 ATF7 PCBP2 LOC100652999 SP1 AAAS CALCOCO1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NPFF on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NPFF

Top Transcription factor binding sites by QIAGEN in the NPFF gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • p53
  • Sp1

Genomic Locations for NPFF Gene

Latest Assembly
chr12:53,506,688-53,507,638
(GRCh38/hg38)
Size:
951 bases
Orientation:
Minus strand

Previous Assembly
chr12:53,900,472-53,901,268
(GRCh37/hg19 by Entrez Gene)
Size:
797 bases
Orientation:
Minus strand

chr12:53,900,474-53,901,422
(GRCh37/hg19 by Ensembl)
Size:
949 bases
Orientation:
Minus strand

Genomic View for NPFF Gene

Genes around NPFF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NPFF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NPFF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NPFF Gene

Proteins for NPFF Gene

  • Protein details for NPFF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O15130-NPFF_HUMAN
    Recommended name:
    Pro-FMRFamide-related neuropeptide FF
    Protein Accession:
    O15130
    Secondary Accessions:
    • Q3SXL4

    Protein attributes for NPFF Gene

    Size:
    113 amino acids
    Molecular mass:
    12440 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for NPFF Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NPFF Gene

Post-translational modifications for NPFF Gene

No Post-translational modifications

Other Protein References for NPFF Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for NPFF Gene

Domains & Families for NPFF Gene

Gene Families for NPFF Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted secreted proteins

Protein Domains for NPFF Gene

InterPro:
Blocks:
  • FMRFamide-related peptide precursor signature

Suggested Antigen Peptide Sequences for NPFF Gene

GenScript: Design optimal peptide antigens:
  • FMRFamide-related peptides (NPFF_HUMAN)
  • Neuropeptide FF-amide peptide, isoform CRA_a (Q3SXL4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O15130

UniProtKB/Swiss-Prot:

NPFF_HUMAN :
  • Belongs to the FARP (FMRFamide related peptide) family.
Family:
  • Belongs to the FARP (FMRFamide related peptide) family.
genes like me logo Genes that share domains with NPFF: view

Function for NPFF Gene

Molecular function for NPFF Gene

UniProtKB/Swiss-Prot Function:
Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels.
GENATLAS Biochemistry:
FMRF-amide related peptides,precursor

Phenotypes From GWAS Catalog for NPFF Gene

Gene Ontology (GO) - Molecular Function for NPFF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001664 G protein-coupled receptor binding IEA,IBA 21873635
GO:0005102 signaling receptor binding TAS 9224703
GO:0005184 neuropeptide hormone activity IBA 21873635
genes like me logo Genes that share ontologies with NPFF: view
genes like me logo Genes that share phenotypes with NPFF: view

Animal Models for NPFF Gene

MGI Knock Outs for NPFF:
  • Npff Npff<tm1.1(KOMP)Vlcg>

Animal Models for research

  • Taconic Biosciences Mouse Models for NPFF

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NPFF

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for NPFF Gene

Localization for NPFF Gene

Subcellular locations from UniProtKB/Swiss-Prot for NPFF Gene

Secreted.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NPFF gene
Compartment Confidence
extracellular 5
nucleus 2
plasma membrane 1

Gene Ontology (GO) - Cellular Components for NPFF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005615 extracellular space IEA,IBA 21873635
GO:0030425 dendrite IEA,IBA 21873635
GO:0031982 vesicle IEA --
GO:0043204 perikaryon IBA 21873635
genes like me logo Genes that share ontologies with NPFF: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for NPFF Gene

Pathways & Interactions for NPFF Gene

genes like me logo Genes that share pathways with NPFF: view

SIGNOR curated interactions for NPFF Gene

Activates:

Gene Ontology (GO) - Biological Process for NPFF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002438 acute inflammatory response to antigenic stimulus IEA --
GO:0003254 regulation of membrane depolarization IEA --
GO:0007186 G protein-coupled receptor signaling pathway TAS --
GO:0007204 positive regulation of cytosolic calcium ion concentration IEA --
GO:0007218 neuropeptide signaling pathway TAS,IEA --
genes like me logo Genes that share ontologies with NPFF: view

Drugs & Compounds for NPFF Gene

(7) Drugs for NPFF Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(7) Additional Compounds for NPFF Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with NPFF: view

Transcripts for NPFF Gene

mRNA/cDNA for NPFF Gene

2 REFSEQ mRNAs :
5 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NPFF

Alternative Splicing Database (ASD) splice patterns (SP) for NPFF Gene

ExUns: 1 ^ 2 ^ 3
SP1:
SP2:

Relevant External Links for NPFF Gene

GeneLoc Exon Structure for
NPFF

Expression for NPFF Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NPFF Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NPFF

SOURCE GeneReport for Unigene cluster for NPFF Gene:

Hs.733076

Evidence on tissue expression from TISSUES for NPFF Gene

  • Blood(2.8)
  • Nervous system(2.7)
genes like me logo Genes that share expression patterns with NPFF: view

Primer products for research

No data available for mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for NPFF Gene

Orthologs for NPFF Gene

This gene was present in the common ancestor of chordates.

Orthologs for NPFF Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia NPFF 29 30
  • 99.41 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia NPFF 29 30
  • 83.19 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Npff 29
  • 77.47 (n)
Mouse
(Mus musculus)
Mammalia Npff 29 16 30
  • 75 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia NPFF 30
  • 41 (a)
OneToOne
Species where no ortholog for NPFF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • Dog (Canis familiaris)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)
  • Zebrafish (Danio rerio)

Evolution for NPFF Gene

ENSEMBL:
Gene Tree for NPFF (if available)
TreeFam:
Gene Tree for NPFF (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NPFF: view image
Alliance of Genome Resources:
Additional Orthologs for NPFF

Paralogs for NPFF Gene

No data available for Paralogs for NPFF Gene

Variants for NPFF Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NPFF Gene

SNP ID Clinical significance and condition Chr 12 pos Variation AA Info Type
rs201195327 Benign: not provided 53,506,882(-) T/C
NM_001320296.2(NPFF):c.245A>G (p.Asn82Ser)
MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS
rs35822762 Benign: not provided 53,506,856(-) A/Gp.Trp88Arg
NM_001320296.2(NPFF):c.271T>C (p.Trp91Arg)
MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT,THREE_PRIME_UTR

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for NPFF Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for NPFF Gene

Variant ID Type Subtype PubMed ID
nsv983486 CNV duplication 23825009

Variation tolerance for NPFF Gene

Residual Variation Intolerance Score: 78% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.26; 40.55% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NPFF Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NPFF
Leiden Open Variation Database (LOVD)
NPFF

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NPFF Gene

Disorders for NPFF Gene

MalaCards: The human disease database

(9) MalaCards diseases for NPFF Gene - From: COP and GCD

Disorder Aliases PubMed IDs
pain agnosia
  • analgesia
agnosia
  • dyspraxia
opioid abuse
  • opioid-related disorders
migraine with aura
  • classic migraine
withdrawal disorder
  • withdrawal syndrome
- elite association - COSMIC cancer census association via MalaCards
Search NPFF in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NPFF

genes like me logo Genes that share disorders with NPFF: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NPFF Gene

Publications for NPFF Gene

  1. A human gene encoding morphine modulating peptides related to NPFF and FMRFamide. (PMID: 9224703) Perry SJ … Burke JF (FEBS letters 1997) 2 3 4 22
  2. Selective modulation of heteromeric ASIC proton-gated channels by neuropeptide FF. (PMID: 11587714) Catarsi S … Séguéla P (Neuropharmacology 2001) 3 4 22
  3. Physical association between neuropeptide FF and micro-opioid receptors as a possible molecular basis for anti-opioid activity. (PMID: 17224450) Roumy M … Mollereau C (The Journal of biological chemistry 2007) 3 22
  4. Regulation of endogenous human NPFF2 receptor by neuropeptide FF in SK-N-MC neuroblastoma cell line. (PMID: 16336216) Ankö ML … Panula P (Journal of neurochemistry 2006) 3 22
  5. Characterization of the NPGP receptor and identification of a novel short mRNA isoform in human hypothalamus. (PMID: 12609745) Laemmle B … Wieland HA (Regulatory peptides 2003) 3 22

Products for NPFF Gene

Sources for NPFF Gene