NOTCH1 Gene (Protein Coding)

Notch Receptor 1

GCID:: GC09M136582

GIFtS:: 52

Genes Participants

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellula... See more...

Aliases for NOTCH1 Gene

Notch Receptor 1 ² ³ ⁵
Notch 1 ² ³ ⁴
Translocation-Associated Notch Protein TAN-1 ³ ⁴
Neurogenic Locus Notch Homolog Protein 1 ³ ⁴
TAN1 ³ ⁴
HN1 ³ ⁴

Notch (Drosophila) Homolog 1 (Translocation-Associated) ²
Notch Homolog 1, Translocation-Associated (Drosophila) ²
Notch Homolog 1, Translocation-Associated ³
EC 3.4.21.68 ⁵²
EC 2.1.2.11 ⁵²
AOVD1 ³
AOS5 ³

External Ids for NOTCH1 Gene

HGNC: 7881
Entrez Gene: 4851
Ensembl: ENSG00000148400
OMIM: 190198
UniProtKB: P46531

Previous HGNC Symbols for NOTCH1 Gene

TAN1

Previous GeneCards Identifiers for NOTCH1 Gene

GC09M130458
GC09M131078
GC09M132829
GC09M134746
GC09M136664
GC09M138508
GC09M139388
GC09M108848
GC09M136495
GC09M136549
GC09M136558
GC09M136562
GC09M136497
GC09M136505
GC09M136513
GC09M136527
GC09M136538

Search aliases for NOTCH1 gene in PubMed and other databases

Summaries for NOTCH1 Gene

Entrez Gene Summary for NOTCH1 Gene

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

CIViC Summary for NOTCH1 Gene

NOTCH1 is one of four known genes encoding the NOTCH family of proteins, a group of receptors involved in the Notch signaling pathway. NOTCH proteins are characterized by N-terminal EGF-like repeats followed by LNR domains which form a complex with ligands to prevent signaling. The Notch signaling pathway is involved in processes related to cell fate specification, differentiation, proliferation, and survival. Activation of Notch has been shown to be correlative with mammary tumorgenesis in mice and increased expression of Notch receptors has been observed in a variety of cancer types including cervical, colon, head and neck, lung, renal, pancreatic, leukemia, and breast cancer. A number of treatment modalities have been explored related to Notch inhibition especially in breast cancer with mixed results.

GeneCards Summary for NOTCH1 Gene

NOTCH1 (Notch Receptor 1) is a Protein Coding gene. Diseases associated with NOTCH1 include Aortic Valve Disease 1 and Adams-Oliver Syndrome 5. Among its related pathways are ERK Signaling and Heart Development. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and sequence-specific DNA binding. An important paralog of this gene is NOTCH2.

UniProtKB/Swiss-Prot Summary for NOTCH1 Gene

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
- NOTC1_HUMAN,P46531

Gene Wiki entry for NOTCH1 Gene

Additional gene information for NOTCH1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NOTCH1 Gene

Genomics for NOTCH1 Gene

GeneHancer (GH) Regulatory Elements for NOTCH1 Gene

Promoters and enhancers for NOTCH1 Gene

GeneHancer (GH) Identifier	GH Type	GH Score	GH Sources	Gene Association Score	Total Score	TSS distance (kb)	Number of Genes Away	Size (kb)	Transcription Factor Binding Sites
GH09J136537	Promoter/Enhancer	2.6	EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER	515.8	+2.6	2587	11.1	SIN3A NRF1 USF1 GTF2E2 PHF8 ZFX ZNF639 ZNF10 ZIC2 POLR2A	NOTCH1 ENSG00000227512 SEC16A ANAPC2 ENTR1 INPP5E SNHG7 NALT1 MAN1B1 EXD3
GH09J136564	Enhancer	1.9	VISTA FANTOM5 ENCODE CraniofacialAtlas dbSUPER	13.4	-22.7	-22747	10.2	CTCF TCF12 USF1 JUND FOS TEAD4 ZNF639 ZIC2 CLOCK GABPA	EXD3 SNHG7 NOTCH1 INPP5E ANAPC2 ENTR1 QSOX2 SEC16A FUT7 TMEM250
GH09J136617	Promoter/Enhancer	1.9	FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER	10.6	-75.6	-75595	9.4	CTCF NCOR1 EP300 ZIC2 KDM6A ZNF423 REST ZBTB25 RAD21 SKI	ANAPC2 EHMT1 DPH7 SEC16A ENTR1 MAN1B1 INPP5E NDOR1 SNAPC4 SNHG7
GH09J136549	Promoter/Enhancer	1.8	FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER	9.6	-5.8	-5795	4.5	JUND FOS ZFX ZIC2 ZNF623 AFF1 RELA CTBP1 MYC TRIM22	INPP5E SEC16A C9orf163 TMEM203 NOTCH1 NALT1 ENTR1 TMEM141 MIR4674 piR-32325-206
GH09J136524	Promoter/Enhancer	1.6	FANTOM5 Ensembl ENCODE dbSUPER	7.3	+15.9	15949	10.3	ZNF10 ZIC2 ZBTB10 ZNF263 ZBTB11 ZNF423 ZNF740 PRPF4 ZNF341 OSR2	NALT1 NOTCH1 DNLZ SEC16A AJM1 PTGDS LCNL1 NACC2 CARD9 lnc-SEC16A-1

- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NOTCH1 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NOTCH1

Top Transcription factor binding sites by QIAGEN in the NOTCH1 gene promoter:

AP-1
ATF-2
c-Fos
c-Jun
NF-kappaB
NF-kappaB1

Genomic Locations for NOTCH1 Gene

chr9:136,494,433-136,546,048: (GRCh38/hg38)

Size:: 51,616 bases
Orientation:: Minus strand

chr9:139,388,896-139,440,314: (GRCh37/hg19)

Size:: 51,419 bases
Orientation:: Minus strand

Genomic View for NOTCH1 Gene

Genes around NOTCH1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:

9q34.3 by HGNC
9q34.3 by Entrez Gene
9q34.3 by Ensembl

NOTCH1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

Genomic Location for NOTCH1 Gene — Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for NOTCH1 Gene

NC_000009.12

Proteins for NOTCH1 Gene

Protein details for NOTCH1 Gene (UniProtKB/Swiss-Prot)
Protein Symbol:

P46531-NOTC1_HUMAN

Recommended name:

Neurogenic locus notch homolog protein 1

Protein Accession:

P46531

Secondary Accessions:
- Q59ED8
- Q5SXM3
Protein attributes for NOTCH1 Gene
Size:

2555 amino acids

Molecular mass:

272505 Da
Quaternary structure:
Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1 (PubMed:11101851, PubMed:12370315). The NOTCH1 intracellular domain interacts with SNW1; the interaction involves multimerized NOTCH1 NICD and is implicated in a formation of an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ (PubMed:10713164). The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation (PubMed:17573339). Interacts (via NICD) with SNAI1 (via zinc fingers); the interaction induces SNAI1 degradation via MDM2-mediated ubiquitination and inhibits SNAI1-induced cell invasion. Interacts (via NICD) with MDM2A. Interacts (via NICD) with BCL6; the interaction decreases MAML1 recruitment by NOTCH1 NICD on target genes DNA and inhibits NOTCH1 transcractivation activity. Interacts with THBS4 (By similarity). Interacts (via the EGF-like repeat region) with CCN3 (via CTCK domain) (PubMed:12050162). Interacts (via EGF-like domains) with DLL4 (via N-terminal DSL and MNNL domains) (By similarity). Interacts with ZMIZ1. Interacts (via NICD domain) with MEGF10 (via the cytoplasmic domain). Interacts with DLL1 and JAG1 (By similarity). Interacts (via NICD domain) with PRAG1 (By similarity). Forms a complex with PRAG1, N1ICD and MAML1, in a MAML1-dependent manner (By similarity). Interacts (via transmembrane region) with PSEN1; the interaction is direct (PubMed:30598546).
Three dimensional structures from OCA and Proteopedia for NOTCH1 Gene
- 1PB5 (3D)
- 1TOZ (3D)
- 1YYH (3D)
- 2F8X (3D)
- 2F8Y (3D)
- 2HE0 (3D)

neXtProt entry for NOTCH1 Gene

Protein Expression for NOTCH1 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for NOTCH1 Gene

P46531:

INTLGSF
CFNGGTC

Post-translational modifications for NOTCH1 Gene

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form (By similarity). Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (PubMed:24226769). Following endocytosis, this fragment is then cleaved by one of the catalytic subunits of gamma-secretase (PSEN1 or PSEN2), to release a Notch-derived peptide containing the intracellular domain (NICD) from the membrane (PubMed:30598546).
- P46531-NOTC1_HUMAN
Phosphorylated.
- P46531-NOTC1_HUMAN
O-glycosylated on the EGF-like domains (PubMed:24226769). O-glucosylated at Ser-435 by KDELC1 and KDELC2 (PubMed:30127001). Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 (By similarity). O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) (PubMed:24226769). MFNG-, RFNG- and LFNG-mediated modification of O-fucose residues at specific EGF-like domains results in inhibition of its activation by JAG1 and enhancement of its activation by DLL1 via an increased binding to DLL1 (By similarity).
- P46531-NOTC1_HUMAN
Ubiquitinated. Undergoes 'Lys-29'-linked polyubiquitination by ITCH; promotes the lysosomal degradation of non-activated internalized NOTCH1 (PubMed:18628966, PubMed:23886940). Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch (PubMed:24226769).
- P46531-NOTC1_HUMAN
Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD (By similarity).
- P46531-NOTC1_HUMAN
Glycosylation at Asn41, Ser65, Thr73, Thr116, Ser146, Thr194, Thr232, Thr311, Ser341, Thr349, Ser378, Ser435, Ser458, Thr466, Ser496, Ser534, Ser609, Thr617, Ser647, Thr692, Ser722, Ser759, Thr767, Ser784, Ser797, Thr805, Ser921, Ser951, Asn959, Thr997, Ser1027, Thr1035, Ser1065, Thr1159, Asn1179, Ser1189, Thr1197, Asn1241, Ser1273, Thr1362, Thr1379, Thr1402, Asn1489, Asn1587, and Thr1725
- NX_P46531 (NX_P46531-1)
Ubiquitination at Lys1759
- NX_P46531 (NX_P46531-1)
Modification sites at PhosphoSitePlus
- P46531

Other Protein References for NOTCH1 Gene

ENSEMBL proteins:

ENSP00000498587

REFSEQ proteins:

Antibody Products

LifeSpan BioSciences NOTCH1 Antibody

G Biosciences Immunotag™ Notch 1 (Cleaved-Val1744) Antibody

Creative Diagnostics Anti-NOTCH1 monoclonal antibody, clone B7

Creative Biolabs

BioLegend

GeneTex Anti-NOTCH1 antibody and Anti-NOTCH1 antibody [3E12]

OriGene Antibodies for NOTCH1
- AP07610SU-N
- AP23350PU-N
- AP31032PU-N
- CF500078
- CF500248
- CF808308
- SM2297PS
- TA300836
- TA312382
- TA319199
- TA319200
- TA321860
- TA321861
- TA336494
- TA336846
- TA347967
- TA354875
- TA500078
- TA500078S
- TA500248
- TA500248S
- TA590258
- TA808308
- TA808308S
Custom Antibody Services

Novus Biologicals Antibodies for NOTCH1

Abcam antibodies for NOTCH1

Invitrogen Antibodies for NOTCH1 (AFLGC-NOTCH1)

Biorbyt antibodies for NOTCH1
- orb126635
- orb158014
- orb227956
- orb256723
- orb312158
- orb373660
- orb401532
- orb538192

Boster Bio Antibodies for NOTCH1
- PA1215
- A00033
- A00033-2
- M00033

Santa Cruz Biotechnology (SCBT) Antibodies for NOTCH1

Sino Biological Antibodies for NOTCH1

Protein Products

BioLegend Recombinant Human Notch-1-Fc Chimera (carrier-free) (777502), Recombinant Human Notch-1-Fc Chimera (carrier-free) (777504), and Recombinant Human Notch-1-Fc Chimera (carrier-free) (777506)

Recombinant Human Notch-1 Fc Chimera Protein, CF (3647-TK)

OriGene Purified Proteins for NOTCH1
Search Origene for MassSpec and Protein Over-expression Lysates for NOTCH1
Origene Custom Protein Services for NOTCH1

Novus Biologicals proteins and lysates for NOTCH1

Sino Biological Recombinant Proteins for NOTCH1
Sino Biological Cell Lysates for NOTCH1

Browse ProSpec Recombinant Proteins

antibodies-online: Show 12 available Notch1 Proteins ranked by validation data
Compare Top Notch1 Proteins

Signalway Proteins for NOTCH1
- AP85729

ProSci Proteins for NOTCH1
- notch 1

Abcam proteins for NOTCH1
- activated Notch1
- Notch1

Assay Products

G Biosciences Immunotag™ Human NOTCH1 (Neurogenic locus notch homolog protein 1) ELISA and Immunotag™ Neurogenic locus notch homolog protein 1 ELISA Kit

RayBiotech Human NOTCH-1 ELISA

Creative Diagnostics NOTCH1 (Human) ELISA Kit and Human Notch-1 ELISA Kit

Aviva Systems Biology NOTCH1 ELISA Kit (Human) : 96 Wells (OKEH00479) and NOTCH1 ELISA Kit (Human) (OKCD08985)

OriGene ELISA Kits for NOTCH1
- EA102443
Custom Assay Services

Human Notch-1 DuoSet ELISA (DY5317)

Novus Biologicals ELISA Kits for NOTCH1

antibodies-online: Show 27 available Notch1 Elisa Kits ranked by validation data
Compare Top Notch1 Elisa Kits
Quality Products:

Boster Bio ELISA Kits for NOTCH1
- EK1619

Signalway ELISA kits for NOTCH1
- EK1844
- EK5779

Browse Sino Biological Assays

Domains & Families for NOTCH1 Gene

Gene Families for NOTCH1 Gene

HGNC:

Human Protein Atlas (HPA):

Cancer-related genes
Disease related genes
Plasma proteins
Potential drug targets
Predicted membrane proteins
Transporters

Protein Domains for NOTCH1 Gene

Blocks:

Ankyrin repeat signature
Aspartic acid and asparagine hydroxylation site
EGF-like calcium-binding
LIN-12/notch repeat (LNR) signature

InterPro:

ProtoNet:

P46531

Suggested Antigen Peptide Sequences for NOTCH1 Gene

GenScript: Design optimal peptide antigens:

Truncated NOTCH1 (A7TW83_HUMAN)
Translocation-associated notch protein TAN-1 (NOTC1_HUMAN)
NOTCH1 protein (Q6IAD4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P46531

UniProtKB/Swiss-Prot:

NOTC1_HUMAN :

Interaction with PSEN1 causes partial unwinding of the transmembrane helix, facilitating access to the scissile peptide bond.
Belongs to the NOTCH family.

Domain:

Interaction with PSEN1 causes partial unwinding of the transmembrane helix, facilitating access to the scissile peptide bond.

Family:

Belongs to the NOTCH family.

Function for NOTCH1 Gene

Molecular function for NOTCH1 Gene

UniProtKB/Swiss-Prot Function:

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

NOTC1_HUMAN,P46531

GENATLAS Biochemistry:

Drosophila Notch homolog 1,mediating cell-cell interactions that specify cell fate during development,undergoing a first proteolytic cleavage by furin (PACE1) in the Golgi during trafficking of Notch to the cell surface,undergoing further cleavage by gamma secretase (see PSEN1) releasing an intracellular domain (NICD) which translocates to the nucleus and modulates transcription of target genes,involved in acute lymphocytic leukemia with translocation t(7;9)(q34;q34.3),expressed in CD34+ hematopoietic cells (see TAN1) and in the developing cochlear duct,inducing delay of hematopoietic differentiation and alteration of cell cycle kinetics

NOTCH1

Enzyme Numbers (IUBMB) for NOTCH1 Gene

EC 2.1.2.11 ⁵²
EC 3.4.21.68 ⁵²

LifeMap Function Summary for NOTCH1 Gene

During embryonic development, NOTCH1 as signaling molecule is secreted from the following cells

Epidermis
- Intermediate Keratinocytes (Embryonic Epidermis)
- Spinous Keratinocytes (Stratified Epidermis)
Cartilage
- Intervertebral Disc Nucleus Pulposus Cells (Nucleus Pulposus)
Primitive Streak
- Notochord Cells (Notochord)

It affects the following cells:

Lateral Plate Mesoderm
- Arterial Endothelial Cells (Paired Dorsal Aorta)
- Hematopoietic Stem Cells (Aorta-Gonad-Mesonephros)
Neural Tube
- Bergmann Glia Precursor Cells (Cerebellar Ventricular Zone)
Heart
Kidney
- Distal Renal Vesicle Cells (Renal Vesicle)
- Proximal Renal Vesicle Cells (Renal Vesicle)
Eye
- Early Retinal Progenitor Cells (Retinal Neuroblastic Layer)
- Late Retinal Progenitor Cells (Retinal Neuroblastic Layer)
Epidermis
- Granular Keratinocytes (Stratified Epidermis)
Primitive Streak
- Hemangioblasts (Posterior Primitive Streak)
Blood
- Hematopoietic Stem Cells (Hematopoietic Bone Marrow)
Spinal Cord
- Oligodendrocyte Precursor Cells (Spinal Cord White Matter)
Paraxial Mesoderm
- Presomitic Mesoderm Cells (Presomitic Mesoderm)

Phenotypes From GWAS Catalog for NOTCH1 Gene

Gene Ontology (GO) - Molecular Function for NOTCH1 Gene

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000978	RNA polymerase II proximal promoter sequence-specific DNA binding	IEA,ISS	--
GO:0001228	DNA-binding transcription activator activity, RNA polymerase II-specific	ISS,IEA	--
GO:0003682	chromatin binding	IEA	--
GO:0003700	DNA-binding transcription factor activity	IEA	--
GO:0004857	enzyme inhibitor activity	ISS,IEA	--

Phenotypes for NOTCH1 Gene

MGI mutant phenotypes for NOTCH1:

inferred from 17 alleles

GenomeRNAi human phenotypes for NOTCH1:

Human Phenotype Ontology for NOTCH1 Gene

HPO Id	HPO Name	Alternative Ids	Definition	Synonyms

Animal Models for NOTCH1 Gene

MGI Knock Outs for NOTCH1:

Notch1 ^{Notch1<tm1Grid>}
Notch1 ^{Notch1<tm1Con>}
Notch1 ^{Notch1<tm2.1Pst>}
Notch1 ^{Notch1<tm2Agt>}
Notch1 ^{Notch1<tm1b(EUCOMM)Hmgu>}
Notch1 ^{Notch1<tm1Pst>}
Notch1 ^{Notch1<tm2.1Rko>}

Animal Model Products

Taconic Biosciences Mouse Models for NOTCH1
- Notch1 - Model 13917 - PM
- Notch1

CRISPR Products

Applied Biological Materials (abm): CRISPR Clones for NOTCH1 - Select products 50% OFF >
* NOTCH1 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
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OriGene CRISPR knockouts for NOTCH1
- GA103238
- GA202936
- KN211365
- KN211365BN
- KN211365LP
- KN211365RB
- KN311124
- KN311124BN
- KN311124LP
- KN311124RB
- KN411365
- KN511124

Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NOTCH1

genomics-online: gRNA clones - Search for available Notch1 gRNA products
Overview of 75 available Notch1 gene related products
Recommended

Santa Cruz Biotechnology (SCBT) CRISPR for NOTCH1

miRNA for NOTCH1 Gene

miRTarBase miRNAs that target NOTCH1

miRNA Products

Search GeneCopoeia for miRNA 3'UTR Target clone products for NOTCH1

Browse OriGene miRNA Products For NOTCH1

Inhibitory RNA Products

Origene shrna, sirna, and RNAi products in human, mouse, rat for NOTCH1
Browse OriGene Inhibitory RNA Products For NOTCH1

Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NOTCH1

genomics-online: shRNA clones - Search for available Notch1 shRNA products
Overview of 75 available Notch1 gene related products
Recommended

Search Santa Cruz Biotechnology (SCBT) for NOTCH1 siRNA/shRNA

Clone Products

Applied Biological Materials (abm): Clones for NOTCH1 - Select products 50% OFF >
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OriGene clones in human,mouse,rat for NOTCH1
- SC215771
- MC225345
- RN206140
- SC308883
OriGene ORF clones in human,mouse,rat for NOTCH1
- MR212150
- RC211365
- RG211365
- RR206140
Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling

Browse Sino Biological cDNA Clones

Addgene plasmids for NOTCH1

Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NOTCH1

genomics-online: cdna clones - Search for available Notch1 CDNA products
Overview of 75 available Notch1 gene related products
Recommended
orf clones - Search for available Notch1 ORF products
Overview of 75 available Notch1 gene related products
Recommended

Human Notch-1 (NP_060087) VersaClone cDNA (RDC2068)

Cell Line Products

Browse ESI BIO Cell Lines and PureStem Progenitors for NOTCH1

Browse GeneCopoeia Cell Lines

VectorBuilder Stable cell line generation for NOTCH1

Horizon Cell Lines for NOTCH1
- NOTCH1 (L1601P/+) HCT116 cell lines
- NOTCH1 knockout cell line

No data available for Transcription Factor Targets and HOMER Transcription for NOTCH1 Gene

Localization for NOTCH1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOTCH1 Gene

Cell membrane; Single-pass type I membrane protein.

P46531-NOTC1_HUMAN

[Notch 1 intracellular domain]: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus. Nuclear location may require MEGF10. {ECO:0000250 UniProtKB:Q01705}.

P46531-NOTC1_HUMAN

Subcellular locations from

COMPARTMENTS

Compartment	Confidence
plasma membrane	5
extracellular	5
nucleus	5
cytosol	5
endoplasmic reticulum	4
golgi apparatus	4
cytoskeleton	3
mitochondrion	3
endosome	2
lysosome	2
peroxisome	1

Subcellular locations from the

Human Protein Atlas (HPA)

Nucleoplasm (2)

See all subcellular structures

Gene Ontology (GO) - Cellular Components for NOTCH1 Gene

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000139	Golgi membrane	TAS	--
GO:0001669	acrosomal vesicle	IEA	--
GO:0002193	MAML1-RBP-Jkappa- ICN1 complex	IDA	16510869
GO:0005576	extracellular region	TAS	--
GO:0005634	nucleus	TAS	10713164

Pathways & Interactions for NOTCH1 Gene

SuperPathways for NOTCH1 Gene

	SuperPathway	Contained pathways
1	Signaling by NOTCH1	Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants .78 Constitutive Signaling by NOTCH1 PEST Domain Mutants .78 Signaling by NOTCH1 .78 Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer .78	Signaling by NOTCH1 in Cancer .78 Signaling by NOTCH1 PEST Domain Mutants in Cancer .78 NOTCH1 Intracellular Domain Regulates Transcription .68 Signaling by NOTCH .65
2	Pre-NOTCH Expression and Processing	Pre-NOTCH Expression and Processing .64 Pre-NOTCH Transcription and Translation .64 Defective LFNG causes SCDO3 .01	Notch-HLH transcription pathway .01 Pre-NOTCH Processing in Golgi .01 Pre-NOTCH Processing in the Endoplasmic Reticulum .01
3	Notch Signaling Pathway (WikiPathways)	Notch signaling pathway .75 Notch Signaling Pathway .75 Notch Signaling Pathway .41	Notch signaling pathway .36 Notch Signaling Pathways .31
4	NOTCH2 Activation and Transmission of Signal to the Nucleus	Activated NOTCH1 Transmits Signal to the Nucleus .58 Constitutive Signaling by NOTCH1 HD Domain Mutants .57 Signaling by NOTCH1 t(7;9)(NOTCH1-M1580 K2555) Translocation Mutant .47	Constitutive Signaling by NOTCH1 t(7;9)(NOTCH1-M1580_K2555) Translocation Mutant .01 Signaling by NOTCH1 HD Domain Mutants in Cancer .01
5	HIV Life Cycle	Disease .45	Diseases of signal transduction .43

Pathways by source for NOTCH1 Gene

1 Sino Biological pathway for NOTCH1 Gene

Notch Signaling Pathway

18 BioSystems pathways for NOTCH1 Gene

31 Reactome pathways for NOTCH1 Gene

9 KEGG pathways for NOTCH1 Gene

2 GeneGo (Thomson Reuters) pathways for NOTCH1 Gene

Development Notch Signaling Pathway
Development NOTCH1-mediated pathway for NF-KB activity modulation

5 R&D Systems pathways for NOTCH1 Gene

5 Qiagen pathways for NOTCH1 Gene

Epithelial Adherens Junctions
Molecular Mechanisms of Cancer
Notch Signaling
Pancreatic Adenocarcinoma
Presenilin-Mediated Signaling

3 Cell Signaling Technology pathways for NOTCH1 Gene

2 GeneTex pathways for NOTCH1 Gene

Interacting Proteins for NOTCH1 Gene

Selected Interacting proteins: P46531-NOTC1_HUMAN for NOTCH1 Gene via UniProtKB MINT IID

Symbol	External ID(s)	Details
RBPJ	Q06330-6 ⁴ Q06330 ⁴ ²⁰ ¹²⁷	EBI-636374, EBI-12599287 EBI-636374, EBI-632552 MINT-8177109 IID: # exp=26 # pred=4
NFKB1	P19838 ⁴ ²⁰ ¹²⁷	EBI-636374, EBI-300010 MINT-2738217 IID: # exp=3 # pred=3
XIAP	P98170 ⁴ ²⁰ ¹²⁷	EBI-636374, EBI-517127 MINT-4543457 IID: # exp=1 # pred=2
ATM	Q13315 ⁴ ¹²⁷	EBI-636374, EBI-495465 IID: # exp=1
CDKN2A	P42771 ¹²⁷ Q8N726 ¹²⁷	IID: # pred=1 IID: # pred=1

GPS-Prot Interaction Network for NOTCH1

;

SIGNOR curated interactions for NOTCH1 Gene

Activates:

Inactivates:

TCF3

Is activated by:

Is inactivated by:

Gene Ontology (GO) - Biological Process for NOTCH1 Gene

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000122	negative regulation of transcription by RNA polymerase II	ISS	--
GO:0001525	angiogenesis	IEA	--
GO:0001701	in utero embryonic development	IEA	--
GO:0001708	cell fate specification	IEA	--
GO:0001837	epithelial to mesenchymal transition	ISS,IEA	--

Drugs & Compounds for NOTCH1 Gene

(26) Drugs for NOTCH1 Gene - From: ApexBio, DGIdb, HMDB, and Novoseek

Name	Status	Disease Links	Group	Role	Mechanism of Action	Clinical Trials
Calcium	Approved	MalaCards Medline Plus	Nutra			7884
PF-03084014		MalaCards Medline Plus	Pharma	other	γ-secretase inhibitor, Other	0
BMS-983970		MalaCards Medline Plus	Pharma			0
FLI-06		MalaCards Medline Plus	Pharma			0
LY3039478		MalaCards Medline Plus	Pharma		Notch inhibitor, novel and potent	0

(8) Additional Compounds for NOTCH1 Gene - From: Novoseek

Name	Synonyms	Role	CAS Number	PubChem IDs	PubMed IDs

(6) ApexBio Compounds for NOTCH1 Gene

Compound	Action	Cas Number
BMS-983970		1584713-87-0
FLI-06		313967-18-9
LY3039478	Notch inhibitor, novel and potent	1421438-81-4
LY-900009	Potent Notch inhibitor	209984-68-9
PF-03084014	γ-secretase inhibitor	865773-15-5
Z-Ile-Leu-aldehyde		161710-10-7

Drug Products

BioLegend Recombinant Human Notch-1-Fc Chimera (carrier-free) (777502), Recombinant Human Notch-1-Fc Chimera (carrier-free) (777504), and Recombinant Human Notch-1-Fc Chimera (carrier-free) (777506)

Browse Tocris compounds for NOTCH1

ApexBio compounds for NOTCH1

Transcripts for NOTCH1 Gene