The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012] See more...

Aliases for NOSIP Gene

Aliases for NOSIP Gene

  • Nitric Oxide Synthase Interacting Protein 2 3 5
  • Nitric Oxide Synthase-Interacting Protein 3 4
  • RING-Type E3 Ubiquitin Transferase NOSIP 3 4
  • E3 Ubiquitin-Protein Ligase NOSIP 3 4
  • ENOS-Interacting Protein 3 4
  • CGI-25 2 3
  • EC 2.3.2.27 4
  • NOSIP 5

External Ids for NOSIP Gene

Previous GeneCards Identifiers for NOSIP Gene

  • GC19M050717
  • GC19M050427
  • GC19M054734
  • GC19M054751
  • GC19M054752
  • GC19M054753
  • GC19M050058
  • GC19M046436

Summaries for NOSIP Gene

Entrez Gene Summary for NOSIP Gene

  • The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]

GeneCards Summary for NOSIP Gene

NOSIP (Nitric Oxide Synthase Interacting Protein) is a Protein Coding gene. Diseases associated with NOSIP include Polyomavirus-Associated Nephropathy. Among its related pathways are eNOS activation and regulation and Nanog in Mammalian ESC Pluripotency.

UniProtKB/Swiss-Prot Summary for NOSIP Gene

  • E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).

Gene Wiki entry for NOSIP Gene

Additional gene information for NOSIP Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NOSIP Gene

Genomics for NOSIP Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for NOSIP Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J049579 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE CraniofacialAtlas 610.7 +9.7 9662 2.8 BRCA1 SP1 CREB1 GATAD2A PRDM10 REST ZNF629 ZNF512 SIX5 LEF1 PRRG2 NOSIP lnc-RRAS-1-001 PRR12 NUP62 ENSG00000268047 PRMT1 PTOV1 HSD17B14 POLD1
GH19J049589 Promoter/Enhancer 2.1 FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 600.7 -1.8 -1781 5.8 RNF2 SP1 HNRNPL CREB1 PRDM10 ZNF629 TFE3 ZNF512 LEF1 SOX13 PRR12 NOSIP piR-41330 POLD1 NUP62 IRF3 ENSG00000268047 PNKP PTOV1-AS2 ALDH16A1
GH19J049558 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE dbSUPER 628.5 +29.5 29537 4.3 EGR1 NFE2 FOXK2 KDM4B ZSCAN29 MNT ZFHX2 BCOR NR2F1 CREM NOSIP lnc-RRAS-1-002 piR-49035 PRR12 RCN3 FCGRT MIR150 RPS11 SNORD35B SNORD34
GH19J049588 Enhancer 0.6 ENCODE 600.7 +1.2 1233 0.2 TCF12 IKZF2 PKNOX1 EED DPF2 RUNX3 SCRT1 BCL11A IKZF1 JUNB NOSIP piR-41330 piR-50444-234 PRRG2
GH19J049552 Promoter/Enhancer 1.4 Ensembl ENCODE CraniofacialAtlas dbSUPER 28.5 +35.0 34983 5.6 GATAD2A KDM1A MTA2 PKNOX1 RXRB SP1 POLR2A SOX13 USF1 GABPA NOSIP PRR12 FCGRT RCN3 PRRG2 MIR150 RPS11 SNORD35B SNORD33 SNORD34
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NOSIP on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NOSIP

Top Transcription factor binding sites by QIAGEN in the NOSIP gene promoter:
  • c-Myb
  • Egr-2
  • Egr-4
  • HOXA5
  • NF-kappaB
  • NF-kappaB1
  • p53
  • RelA
  • STAT5A
  • Zic3

Genomic Locations for NOSIP Gene

Genomic Locations for NOSIP Gene
chr19:49,555,468-49,590,262
(GRCh38/hg38)
Size:
34,795 bases
Orientation:
Minus strand
chr19:50,058,968-50,093,519
(GRCh37/hg19)
Size:
34,552 bases
Orientation:
Minus strand

Genomic View for NOSIP Gene

Genes around NOSIP on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NOSIP Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NOSIP Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NOSIP Gene

Proteins for NOSIP Gene

  • Protein details for NOSIP Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y314-NOSIP_HUMAN
    Recommended name:
    Nitric oxide synthase-interacting protein
    Protein Accession:
    Q9Y314
    Secondary Accessions:
    • Q96FD2

    Protein attributes for NOSIP Gene

    Size:
    301 amino acids
    Molecular mass:
    33172 Da
    Quaternary structure:
    • Interacts with NOS1 and NOS3 (PubMed:11149895, PubMed:15548660). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (By similarity).

neXtProt entry for NOSIP Gene

Post-translational modifications for NOSIP Gene

  • Ubiquitination at Lys117, Lys157, Lys253, and Lys294
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for NOSIP Gene

Domains & Families for NOSIP Gene

Gene Families for NOSIP Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for NOSIP Gene

InterPro:
Blocks:
  • Zn-finger, modified RING
ProtoNet:

Suggested Antigen Peptide Sequences for NOSIP Gene

GenScript: Design optimal peptide antigens:
  • eNOS-interacting protein (NOSIP_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9Y314

UniProtKB/Swiss-Prot:

NOSIP_HUMAN :
  • The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.
  • Belongs to the NOSIP family.
Domain:
  • The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.
Family:
  • Belongs to the NOSIP family.
genes like me logo Genes that share domains with NOSIP: view

Function for NOSIP Gene

Molecular function for NOSIP Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27;.

Enzyme Numbers (IUBMB) for NOSIP Gene

Phenotypes From GWAS Catalog for NOSIP Gene

Gene Ontology (GO) - Molecular Function for NOSIP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding HDA 22658674
GO:0004842 ubiquitin-protein transferase activity IEA --
GO:0005515 protein binding IPI 11149895
GO:0016740 transferase activity IEA --
GO:0061630 ubiquitin protein ligase activity IEA --
genes like me logo Genes that share ontologies with NOSIP: view
genes like me logo Genes that share phenotypes with NOSIP: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NOSIP

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NOSIP Gene

Localization for NOSIP Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOSIP Gene

Cytoplasm. Nucleus. Note=Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813).

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NOSIP gene
Compartment Confidence
nucleus 5
cytosol 4
golgi apparatus 4
plasma membrane 2
extracellular 2
cytoskeleton 1
mitochondrion 1
endoplasmic reticulum 1
endosome 1
lysosome 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for NOSIP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005634 nucleus IBA,IDA 15548660
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA,IDA 15548660
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with NOSIP: view

Pathways & Interactions for NOSIP Gene

genes like me logo Genes that share pathways with NOSIP: view

Pathways by source for NOSIP Gene

1 Qiagen pathway for NOSIP Gene
  • eNOS Signaling

Gene Ontology (GO) - Biological Process for NOSIP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007275 multicellular organism development IEA --
GO:0016567 protein ubiquitination IEA --
GO:0043086 negative regulation of catalytic activity IDA 11149895
GO:0050999 regulation of nitric-oxide synthase activity TAS --
GO:0051001 negative regulation of nitric-oxide synthase activity IDA 11149895
genes like me logo Genes that share ontologies with NOSIP: view

No data available for SIGNOR curated interactions for NOSIP Gene

Drugs & Compounds for NOSIP Gene

No Compound Related Data Available

Transcripts for NOSIP Gene

mRNA/cDNA for NOSIP Gene

3 REFSEQ mRNAs :
12 NCBI additional mRNA sequence :
16 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NOSIP

Alternative Splicing Database (ASD) splice patterns (SP) for NOSIP Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b
SP1: - - - - - -
SP2: - - - - - - -
SP3: - - - - -
SP4: - - - -
SP5: - - - - - -
SP6: - - - - - - - -
SP7:
SP8: - - - -
SP9: -
SP10: - -

Relevant External Links for NOSIP Gene

GeneLoc Exon Structure for
NOSIP

Expression for NOSIP Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NOSIP Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NOSIP Gene

This gene is overexpressed in Whole Blood (x4.3).

Protein differential expression in normal tissues from HIPED for NOSIP Gene

This gene is overexpressed in Breast (43.4) and Lymph node (7.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NOSIP Gene



Protein tissue co-expression partners for NOSIP Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NOSIP

SOURCE GeneReport for Unigene cluster for NOSIP Gene:

Hs.7236

mRNA Expression by UniProt/SwissProt for NOSIP Gene:

Q9Y314-NOSIP_HUMAN
Tissue specificity: Expressed in heart, brain and lung. Present in endothelial cells (at protein level).

Evidence on tissue expression from TISSUES for NOSIP Gene

  • Nervous system(4.7)
  • Liver(4.6)
  • Lung(3.3)
  • Muscle(2.8)
  • Heart(2.5)
  • Skin(2.4)
  • Eye(2.2)
  • Blood(2.1)
  • Kidney(2.1)
  • Intestine(2.1)
genes like me logo Genes that share expression patterns with NOSIP: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for NOSIP Gene

Orthologs for NOSIP Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NOSIP Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia NOSIP 30 31
  • 98.89 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia NOSIP 30 31
  • 91.03 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia NOSIP 30 31
  • 88.26 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Nosip 30
  • 85.94 (n)
Mouse
(Mus musculus)
Mammalia Nosip 30 17 31
  • 85.83 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 84 (a)
OneToMany
-- 31
  • 38 (a)
OneToMany
Oppossum
(Monodelphis domestica)
Mammalia NOSIP 31
  • 84 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia NOSIP 31
  • 78 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia nosip 30
  • 65.88 (n)
MGC75989 30
Zebrafish
(Danio rerio)
Actinopterygii nosip 30 31
  • 63.41 (n)
OneToOne
-- 30
Fruit Fly
(Drosophila melanogaster)
Insecta CG6179 30 31 32
  • 59.8 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005654 30
  • 58.97 (n)
Worm
(Caenorhabditis elegans)
Secernentea R05G6.4 30 31
  • 48.51 (n)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons AT1G61620 30
  • 44.29 (n)
Rice
(Oryza sativa)
Liliopsida Os03g0279900 30
  • 46.16 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 50 (a)
OneToOne
Species where no ortholog for NOSIP was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for NOSIP Gene

ENSEMBL:
Gene Tree for NOSIP (if available)
TreeFam:
Gene Tree for NOSIP (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NOSIP: view image

Paralogs for NOSIP Gene

No data available for Paralogs for NOSIP Gene

Variants for NOSIP Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NOSIP Gene

SNP ID Clinical significance and condition Chr 19 pos Variation AA Info Type
rs17850728 - p.Thr168Met

Additional dbSNP identifiers (rs#s) for NOSIP Gene

Structural Variations from Database of Genomic Variants (DGV) for NOSIP Gene

Variant ID Type Subtype PubMed ID
nsv1160655 CNV deletion 26073780
nsv469715 CNV gain+loss 16826518
nsv828578 CNV loss 20364138
nsv833862 CNV loss 17160897
nsv833863 CNV loss 17160897
nsv833864 CNV gain 17160897
nsv833865 CNV gain+loss 17160897
nsv953600 CNV deletion 24416366
nsv9739 CNV gain+loss 18304495

Variation tolerance for NOSIP Gene

Residual Variation Intolerance Score: 26.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.42; 9.29% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NOSIP Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NOSIP

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NOSIP Gene

Disorders for NOSIP Gene

MalaCards: The human disease database

(1) MalaCards diseases for NOSIP Gene - From: DISEASES

Disorder Aliases PubMed IDs
polyomavirus-associated nephropathy
  • polyomavirus associated nephropathy
- elite association - COSMIC cancer census association via MalaCards
Search NOSIP in MalaCards View complete list of genes associated with diseases
genes like me logo Genes that share disorders with NOSIP: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NOSIP Gene

Publications for NOSIP Gene

  1. Cell cycle-regulated inactivation of endothelial NO synthase through NOSIP-dependent targeting to the cytoskeleton. (PMID: 16135813) Schleicher M … Oess S (Molecular and cellular biology 2005) 3 4 23
  2. NOSIP, a novel modulator of endothelial nitric oxide synthase activity. (PMID: 11149895) Dedio J … Müller-Esterl W (FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001) 2 3 4
  3. Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. (PMID: 10810093) Lai CH … Lin W (Genome research 2000) 2 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. Nitric oxide synthase (NOS)-interacting protein interacts with neuronal NOS and regulates its distribution and activity. (PMID: 15548660) Dreyer J … Kuner R (The Journal of neuroscience : the official journal of the Society for Neuroscience 2004) 3 4

Products for NOSIP Gene

  • Signalway ELISA kits for NOSIP
  • Signalway Proteins for NOSIP

Sources for NOSIP Gene