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Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Mageni... See more...
Aliases for NOS2 Gene
Aliases for NOS2 Gene
External Ids for NOS2 Gene
- HGNC: 7873
- Entrez Gene: 4843
- Ensembl: ENSG00000007171
- OMIM: 163730
- UniProtKB: P35228
Previous HGNC Symbols for NOS2 Gene
- NOS2A
Previous GeneCards Identifiers for NOS2 Gene
- GC17M023108
- GC17M026083
- GC17M022290
Summaries for NOS2 Gene
-
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
GeneCards Summary for NOS2 Gene
NOS2 (Nitric Oxide Synthase 2) is a Protein Coding gene. Diseases associated with NOS2 include Malaria and Meningioma, Radiation-Induced. Among its related pathways are Tuberculosis and VEGF Signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and oxidoreductase activity. An important paralog of this gene is NOS1.
UniProtKB/Swiss-Prot Summary for NOS2 Gene
-
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Additional gene information for NOS2 Gene
- HGNC(7873)
- Entrez Gene(4843)
- Ensembl(ENSG00000007171)
- OMIM(163730)
- UniProtKB(P35228)
- Open Targets Platform(ENSG00000007171)
- Monarch Initiative
- Search for NOS2 at DataMed
- Search for NOS2 at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NOS2 Gene
Genomics for NOS2 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for NOS2 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around NOS2 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NOS2
- Top Transcription factor binding sites by QIAGEN in the NOS2 gene promoter:
-
- HNF-3beta
- HNF-4alpha1
- IRF-1
- MyoD
- p300
Genomic Locations for NOS2 Gene
Genomic Locations for NOS2 Gene
- chr17:27,756,766-27,800,529
- (GRCh38/hg38)
- Size:
- 43,764 bases
- Orientation:
- Minus strand
- chr17:26,083,792-26,127,555
- (GRCh37/hg19)
- Size:
- 43,764 bases
- Orientation:
- Minus strand
Genomic View for NOS2 Gene
Genes around NOS2 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 17q11.2 by HGNC
- 17q11.2 by Entrez Gene
- 17q11.2 by Ensembl
NOS2 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for NOS2 Gene
Proteins for NOS2 Gene
-
Protein details for NOS2 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P35228-NOS2_HUMAN
- Recommended name:
- Nitric oxide synthase, inducible
- Protein Accession:
- P35228
- A1L3U5
- B7ZLY2
- O60757
- O94994
- Q16263
- Q16692
- Q4TTS5
- Q9UD42
Protein attributes for NOS2 Gene
- Size:
- 1153 amino acids
- Molecular mass:
- 131117 Da
- Cofactor:
- Name=heme; Xref=ChEBI:CHEBI:30413;
- Cofactor:
- Name=FAD; Xref=ChEBI:CHEBI:57692;
- Cofactor:
- Name=FMN; Xref=ChEBI:CHEBI:58210;
- Cofactor:
- Name=(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin; Xref=ChEBI:CHEBI:59560;
- Quaternary structure:
-
- Homodimer (PubMed:10409685, PubMed:10074942). Interacts with SLC9A3R1 (PubMed:12080081). Interacts with GAPDH; induced by oxidatively-modified low-densitity lipoprotein (LDL(ox)) (PubMed:25417112). Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (PubMed:25417112). Interacts with SPSB1, SPSB2 and SPSB4 (PubMed:21199876). Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4 (PubMed:21199876).
Three dimensional structures from OCA and Proteopedia for NOS2 Gene
Protein Expression for NOS2 Gene
Selected DME Specific Peptides for NOS2 Gene
- P35228:
-
- VLEEFPS
- YSRLPGKPK
- SRQNLQSP
- RGGCPADW
- EEQVEDYFFQLK
- GWYMGTE
- LQVFDAR
- SFKEAKIEE
- EFTQLCIDLGWKP
- GPLHHGVCSTW
- AWRNAPRC
- DGKHDFR
- GDPANVE
- PFNGWYM
- VEINIAVL
- FEIPPDLVLEV
- RSAITVFPQR
- ELLPQAIEF
- KSLTRGPRDKPTP
- VTKEIETTGTY
- VVTSTFGNGD
- WKTHVWQDE
- CGDVRMARDVA
- FRYAVFGLGSSMYP
- EVGRRMGLE
- FLLSQLPILKPR
- FCAFAHDID
- LDITTPP
- EMFEHICRH
- GNIRSAIT
- DHHSAAESFMK
- MTLVFGCR
- QLIRYAGY
- DTLHHKA
- ATETGKS
- RQRLEALCQPSEY
- EIHLTVA
- QALTYFLDIT
- AVLHSFQKQNVTIMDHH
- LKWYALP
- TPVFHQEM
- APFRSFWQQR
- MEHPKYEWF
Post-translational modifications for NOS2 Gene
- Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
- Modification sites at PhosphoSitePlus
- Modification sites at neXtProt
Other Protein References for NOS2 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- LifeSpan BioSciences NOS2 / iNOS Monoclonal Antibody
- Creative Diagnostics Rabbit Anti-Mouse iNOS monoclonal antibody, clone TQ237 and Anti-NOS2 monoclonal antibody, clone FN-40
-
Creative Biolabs
- Recombinant Anti-Human NOS2 Antibody
- Recombinant Human Anti-NOS2 Antibody (1E8-B8)
- Mouse Anti-NOS2 Recombinant Antibody (NOS-3F7-B11 B5)
- Mouse Anti-NOS2 Recombinant Antibody (5C1B52)
- Mouse Anti-IgY Recombinant Antibody (CBYY-I0745)
- Mouse Anti-NOS2 Recombinant Antibody (CBYY-I1243)
- Mouse Anti-NOS2 Recombinant Antibody (4E5)
- Recombinant Human Anti-NOS2 Antibody scFv Fragment (24H9-1F3)
- HUABIO iNOS Antibody (ER1706-89)
-
Custom Antibody ServicesOriGene Antibodies for NOS2
- Novus Biologicals Antibodies for NOS2
-
Abcam antibodies for NOS2
- Invitrogen Antibodies for NOS2 (AFLGC-NOS2)
- antibodies-online: Show 108 available NOS2 Antibodies ranked by validation data
- Compare Top NOS2 Antibodies
-
Recommended
- 203 WB Antibodies (anti-human)
- 19 IHC (p) Antibodies (anti-human)
- 15 IHC Antibodies (anti-human)
- 12 IF (p) Antibodies (anti-human)
- 4 IP Antibodies (anti-human)
- 3 FACS Antibodies (anti-human)
- 1 IF Antibody (anti-human)
- 121 WB Antibodies (anti-mouse)
- 13 IHC Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 11 IHC (p) Antibodies (anti-mouse)
- 3 FACS Antibodies (anti-mouse)
- 71 WB Antibodies (anti-rat)
- 12 IF (p) Antibodies (anti-rat)
- 8 IHC Antibodies (anti-rat)
- 8 IHC (p) Antibodies (anti-rat)
- 4 IP Antibodies (anti-rat)
-
Boster Bio Antibodies for NOS2
-
Santa Cruz Biotechnology (SCBT) Antibodies for NOS2
- Sino Biological Antibodies for NOS2
Protein Products
- Search Origene for MassSpec and Protein Over-expression Lysates for NOS2
- Origene Custom Protein Services for NOS2
-
Abcam proteins for NOS2
Assay Products
- Creative Diagnostics NOS (Human) ELISA Kit and Human/Rat NO Synthase I ELISA Kit
- Novus Biologicals ELISA Kits for NOS2
- antibodies-online: Show 101 available NOS2 Elisa Kits ranked by validation data
- Compare Top NOS2 Elisa Kits
-
Quality Products:
- ABIN6730909
- ABIN1115495
- ABIN5526682
- ABIN415084
- ABIN365871
- ABIN1571522
- ABIN4947903
- ABIN4947902
- ABIN5522466
- ABIN955952
- ABIN6574224
- ABIN415396
- ABIN1115494
- ABIN366306
- ABIN455420
- ABIN1571523
- ABIN5522467
- ABIN5670001
- ABIN5593187
- ABIN6574225
- ABIN416102
- ABIN1115493
- ABIN367490
- ABIN579874
- ABIN1571524
- ABIN5522469
- ABIN5670665
- ABIN5593188
- ABIN431312
- ABIN368550
- ABIN1571525
- ABIN5522468
- ABIN1564704
- ABIN775151
- ABIN5657404
- ABIN2949938
- ABIN2537260
- ABIN6211012
-
Boster Bio ELISA Kits for NOS2
Domains & Families for NOS2 Gene
Gene Families for NOS2 Gene
- IUPHAR :
- Human Protein Atlas (HPA):
-
- Cancer-related genes
- Enzymes
- FDA approved drug targets
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for NOS2 Gene
- InterPro:
- Blocks:
-
- Oxidoreductase FAD/NAD(P)-binding
- Flavoprotein pyridine nucleotide cytochrome reductase signature
- Flavodoxin signature
- Flavodoxin/nitric oxide synthase
- FAD-binding
- Nitric oxide synthase, NOS
- ProtoNet:
Suggested Antigen Peptide Sequences for NOS2 Gene
- GenScript: Design optimal peptide antigens:
-
- Peptidyl-cysteine S-nitrosylase NOS2 (NOS2_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P35228- Family:
-
- Belongs to the NOS family.
Function for NOS2 Gene
Molecular function for NOS2 Gene
- UniProtKB/Swiss-Prot Function:
- Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide; Xref=Rhea:RHEA:19897, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16480, ChEBI:CHEBI:32682, ChEBI:CHEBI:57743, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.14.13.39;.
- UniProtKB/Swiss-Prot Induction:
- By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta (PubMed:7528267). Down-regulated by zinc due to inhibition of NF-kappa-B transactivation activity (PubMed:25180171). By oxidatively-modified low-densitity lipoprotein (LDL(ox)) (PubMed:25417112).
- UniProtKB/Swiss-Prot EnzymeRegulation:
- Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity (By similarity).
Enzyme Numbers (IUBMB) for NOS2 Gene
Phenotypes From GWAS Catalog for NOS2 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0003958 | NADPH-hemoprotein reductase activity | IBA | 21873635 |
| GO:0004517 | nitric-oxide synthase activity | IBA,IDA | 7504305 |
| GO:0005515 | protein binding | IPI | 12855682 |
| GO:0005516 | calmodulin binding | IEA | -- |
| GO:0010181 | FMN binding | ISS | -- |
Phenotypes for NOS2 Gene
- MGI mutant phenotypes for NOS2:
-
inferred from 4 alleles
- nervous system phenotype
- immune system phenotype
- respiratory system phenotype
- cardiovascular system phenotype
- cellular phenotype
- behavior/neurological phenotype
- mortality/aging
- muscle phenotype
- homeostasis/metabolism phenotype
- growth/size/body region phenotype
- liver/biliary system phenotype
- renal/urinary system phenotype
- neoplasm
- digestive/alimentary phenotype
- endocrine/exocrine gland phenotype
- reproductive system phenotype
- vision/eye phenotype
- skeleton phenotype
- hematopoietic system phenotype
- integument phenotype
- craniofacial phenotype
- adipose tissue phenotype
- GenomeRNAi human phenotypes for NOS2:
-
- no effect
- Synthetic lethal with MLN4924 (a NAE inhibitor)
- Increased viability with MLN4924 (a NAE inhibitor)
- Increased shRNA abundance (Z-score > 2)
- Decreased shRNA abundance (Z-score < -2)
- Increased gamma-H2AX phosphorylation
- shRNA abundance <= 25%
- Increased viability
- Decreased shRNA abundance
- Decreased viability after Chlamydia trachomatis serovar L2 infection and TNF-alpha/CHX stimulation
Animal Models for NOS2 Gene
Animal Model Products
-
Taconic Biosciences Mouse Models for NOS2
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for NOS2 - Select products 50% OFF >
- * NOS2 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for NOS2
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NOS2
-
Santa Cruz Biotechnology (SCBT) CRISPR for NOS2
miRNA for NOS2 Gene
- miRTarBase miRNAs that target NOS2
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for NOS2
- Browse OriGene Inhibitory RNA Products For NOS2
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NOS2
Clone Products
- Applied Biological Materials (abm): Clones for NOS2 - Select products 50% OFF >
- * NOS2 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
- Sino Biological Human cDNA Clone for NOS2
- Addgene plasmids for NOS2
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NOS2
Cell Line Products
-
Horizon Cell Lines for NOS2
No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NOS2 Gene
Localization for NOS2 Gene
Subcellular locations from UniProtKB/Swiss-Prot for NOS2 Gene
- Cytoplasm, cytosol. Note=Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. {ECO:0000269 PubMed:21199876}.
| Compartment | Confidence |
|---|---|
| peroxisome | 5 |
| plasma membrane | 4 |
| nucleus | 4 |
| cytosol | 4 |
| extracellular | 3 |
| cytoskeleton | 3 |
| mitochondrion | 3 |
| endoplasmic reticulum | 2 |
| endosome | 2 |
| lysosome | 2 |
| golgi apparatus | 1 |
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005623 | cell | IEA | -- |
| GO:0005634 | nucleus | IBA,ISS | -- |
| GO:0005737 | cytoplasm | IEA,IDA | 9916929 |
| GO:0005777 | peroxisome | IDA | 12085352 |
| GO:0005782 | peroxisomal matrix | TAS | -- |
No data available for Subcellular locations from the Human Protein Atlas (HPA) for NOS2 Gene
Pathways & Interactions for NOS2 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Arginine biosynthesis | ||
| 2 | Toxoplasmosis |
.34
|
.34
|
| 3 | G-Beta Gamma Signaling |
CRHR Pathway
.44
|
Relaxin Pathway
.37
|
| 4 | PEDF Induced Signaling |
MIF Regulation of Innate Immune Cells
.94
|
Endothelin-1 Signaling Pathway
.46
|
| 5 | Toll Comparative Pathway |
Immune response MIF in innate immunity response
.46
|
iNOS Signaling
.34
|
Pathways by source for NOS2 Gene
1 Sino Biological pathway for NOS2 Gene
15 BioSystems pathways for NOS2 Gene
9 Reactome pathways for NOS2 Gene
3 PharmGKB pathways for NOS2 Gene
17 KEGG pathways for NOS2 Gene
11 GeneGo (Thomson Reuters) pathways for NOS2 Gene
- Bacterial infections in CF airways
- Development VEGF signaling via VEGFR2 - generic cascades
- G-protein signaling_Rac2 regulation pathway
- Immune response CCR3 signaling in eosinophils
- Immune response Bacterial infections in normal airways
10 Qiagen pathways for NOS2 Gene
- AMPK Enzyme Complex Pathway
- Colorectal Cancer Metastasis
- CRHR Pathway
- Endothelin-1 Signaling Pathway
- ILK Signaling
Interacting Proteins for NOS2 Gene
Selected Interacting proteins:
P35228-NOS2_HUMAN
ENSP00000327251
for NOS2 Gene via
UniProtKB
STRING
IID
GPS-Prot Interaction Network for NOS2
SIGNOR curated interactions for NOS2 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0001666 | response to hypoxia | IEA | -- |
| GO:0001912 | positive regulation of leukocyte mediated cytotoxicity | TAS | 10450191 |
| GO:0002227 | innate immune response in mucosa | NAS | 18390757 |
| GO:0006527 | arginine catabolic process | IBA,IDA | 7504305 |
| GO:0006625 | protein targeting to peroxisome | TAS | -- |
Drugs & Compounds for NOS2 Gene
(133) Drugs for NOS2 Gene - From: DrugBank, PharmGKB, ApexBio, DGIdb, IUPHAR, HMDB, Tocris, and Novoseek
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Dexamethasone | Approved, Investigational, Vet_approved | Pharma | Agonist, Target, negative modulator | Anti-inflammatory glucocorticoid | 2886 | |
| Citrulline | Approved, Investigational | Nutra | Target | 0 | ||
| Miconazole | Approved, Investigational, Vet_approved | Pharma | Channel blocker, Pore Blocker, Antagonist, Target, inhibitor | 3024 | ||
| Minocycline | Approved, Investigational | Pharma | Target, inhibitor | 229 | ||
| Nitric Oxide | Approved | Pharma | 771 |
| Name | Synonyms | Role | CAS Number | PubChem IDs | PubMed IDs | |
|---|---|---|---|---|---|---|
| Hydrogen Ion |
|
|
||||
| N(omega)-Hydroxyarginine |
|
53054-07-2 |
|
(5) Tocris Compounds for NOS2 Gene
| Compound | Action | Cas Number |
|---|---|---|
| (S)-Methylisothiourea sulfate | Highly selective iNOS inhibitor | 867-44-7 |
| Aminoguanidine hydrochloride | Irreversible iNOS inhibitor | 1937-19-5 |
| AMT hydrochloride | Potent, selective iNOS inhibitor | 21463-31-0 |
| EIT hydrobromide | Selective iNOS inhibitor, acts arginine binding site | 1071-37-0 |
| S-Isopropylisothiourea hydrobromide | iNOS inhibitor, acts at arginine binding site | 4269-97-0 |
(22) ApexBio Compounds for NOS2 Gene
| Compound | Action | Cas Number |
|---|---|---|
| (S)-Methylisothiourea sulfate | 867-44-7 | |
| 1400W dihydrochloride | INOS inhibitor,potent and highly selective | 214358-33-5 |
| 2,4-Diamino-6-hydroxypyrimidine | 1956-06-4 | |
| 2-Iminopiperidine hydrochloride | 16011-96-4 | |
| 3-Bromo-7-nitroindazole | 74209-34-0 | |
| 7-NINA | 161467-34-1 | |
| 7-Nitroindazole | 2942-42-9 | |
| Aminoguanidine hydrochloride | 1937-19-5 | |
| AMT hydrochloride | 21463-31-0 | |
| AR-C 102222 | 253771-21-0 | |
| ARL 17477 dihydrochloride | Selective nNOS inhibitor | 866914-87-6 |
| BYK 191023 dihydrochloride | INOS inhibitor,potent and selective | 1216722-25-6 |
| Carboxy-PTIO, potassium salt | 148819-94-7 | |
| DMPO | 3317-61-1 | |
| EIT hydrobromide | 1071-37-0 | |
| L-Canavanine sulfate | 2219-31-0 | |
| L-NIL hydrochloride | 150403-89-7 | |
| L-NMMA acetate | 53308-83-1 | |
| ODQ | 41443-28-1 | |
| Saikosaponin A | COX-2 and iNOS inhibitor | 20736-09-8 |
| S-Isopropylisothiourea hydrobromide | 4269-97-0 | |
| TRIM | 25371-96-4 |
Transcripts for NOS2 Gene
mRNA/cDNA for NOS2 Gene
- 2 REFSEQ mRNAs :
- 14 NCBI additional mRNA sequence :
- 3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000313735 4206 nts (view in UCSC)
- ENST00000621962 3345 nts (view in UCSC)
- ENST00000646938 3995 nts (view in UCSC)
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for NOS2 - Select products 50% OFF >
- * NOS2 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for NOS2
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NOS2
-
Santa Cruz Biotechnology (SCBT) CRISPR for NOS2
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for NOS2
- Browse OriGene Inhibitory RNA Products For NOS2
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NOS2
Clone Products
- Applied Biological Materials (abm): Clones for NOS2 - Select products 50% OFF >
- * NOS2 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
- Sino Biological Human cDNA Clone for NOS2
- Addgene plasmids for NOS2
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NOS2
| ExUns: | 1 | ^ | 2a | · | 2b | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 | ^ | 15 | ^ | 16 | ^ | 17 | ^ | 18 | ^ | 19 | ^ | 20 | ^ | 21 | ^ | 22 | ^ | 23 | ^ | 24 | ^ | 25 | ^ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP2: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP3: | - |
| ExUns: | 26 | ^ | 27 | ^ | 28 |
|---|---|---|---|---|---|
| SP1: | |||||
| SP2: | |||||
| SP3: |
Relevant External Links for NOS2 Gene
- GeneLoc Exon Structure for
- NOS2
Expression for NOS2 Gene
mRNA expression in embryonic tissues and stem cells from LifeMap Discovery
- Neural Ectoderm (Nervous System)
- Bone (Muscoskeletal System)
- Cartilage (Muscoskeletal System)
- Brain (Nervous System)
- Neural Tube (Nervous System)
-
Colon (Gastrointestinal Tract)
mRNA differential expression in normal tissues according to GTEx for NOS2 Gene
This gene is overexpressed in Small Intestine - Terminal Ileum (x8.6), Colon - Transverse (x7.9), and Brain - Cortex (x5.0).
This gene is overexpressed in Monocytes (35.0), Heart (13.8), and Neutrophil (9.1).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for NOS2 Gene
Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NOS2
SOURCE GeneReport for Unigene cluster for NOS2 Gene:
Hs.709191mRNA Expression by UniProt/SwissProt for NOS2 Gene:
P35228-NOS2_HUMAN
Tissue specificity:
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Evidence on tissue expression from TISSUES for NOS2 Gene
- Liver(4.6)
- Kidney(4.5)
- Lung(4.5)
- Eye(4.4)
- Blood(3.3)
- Nervous system(3.3)
- Heart(3.3)
- Muscle(3.2)
- Intestine(3)
- Spleen(2.6)
- Lymph node(2.5)
- Skin(2.4)
- Pancreas(2.3)
- Stomach(2.3)
- Bone marrow(2.3)
- Bone(2)
Primer Products
-
OriGene qPCR primer pairs for NOS2
Gene Expression Assay Products
No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for NOS2 Gene
Orthologs for NOS2 Gene
This gene was present in the common ancestor of animals and fungi.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| Chimpanzee (Pan troglodytes) |
Mammalia | NOS2 30 31 |
|
OneToOne | |
| Cow (Bos Taurus) |
Mammalia | NOS2 30 |
|
||
| INOS 31 |
|
OneToOne | |||
| Dog (Canis familiaris) |
Mammalia | NOS2 30 31 |
|
OneToOne | |
| Rat (Rattus norvegicus) |
Mammalia | LOC497963 30 |
|
||
| Mouse (Mus musculus) |
Mammalia | Nos2 30 17 31 |
|
OneToOne | |
| Oppossum (Monodelphis domestica) |
Mammalia | NOS2 31 |
|
OneToOne | |
| Platypus (Ornithorhynchus anatinus) |
Mammalia | NOS2 31 |
|
OneToOne | |
| Chicken (Gallus gallus) |
Aves | NOS2 30 31 |
|
OneToOne | |
| Lizard (Anolis carolinensis) |
Reptilia | NOS2 31 |
|
OneToOne | |
| Tropical Clawed Frog (Silurana tropicalis) |
Amphibia | nos2 30 |
|
||
| Zebrafish (Danio rerio) |
Actinopterygii | nos2b 30 31 |
|
OneToMany | |
| nos2a 31 |
|
OneToMany | |||
| Fruit Fly (Drosophila melanogaster) |
Insecta | Nos 31 |
|
OneToMany | |
| Baker's yeast (Saccharomyces cerevisiae) |
Saccharomycetes | NCP1 31 |
|
OneToMany | |
| Sea Squirt (Ciona savignyi) |
Ascidiacea | CSA.5751 31 |
|
OneToMany |
- Species where no ortholog for NOS2 was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Eremothecium gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- African clawed frog (Xenopus laevis)
- African malaria mosquito (Anopheles gambiae)
- Alicante grape (Vitis vinifera)
- Alpha proteobacteria (Wolbachia pipientis)
- Amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- Barley (Hordeum vulgare)
- Beta proteobacteria (Neisseria meningitidis)
- Bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- Common water flea (Daphnia pulex)
- Corn (Zea mays)
- E. coli (Escherichia coli)
- Filamentous fungi (Aspergillus nidulans)
- Firmicute Bacteria (Streptococcus pneumoniae)
- Fission Yeast (Schizosaccharomyces pombe)
- Green Algae (Chlamydomonas reinhardtii)
- Honey Bee (Apis mellifera)
- K. Lactis Yeast (Kluyveromyces lactis)
- Loblloly Pine (Pinus taeda)
- Malaria Parasite (Plasmodium falciparum)
- Medicago Trunc (Medicago Truncatula)
- Moss (Physcomitrella patens)
- Orangutan (Pongo pygmaeus)
- Pig (Sus scrofa)
- Rainbow Trout (Oncorhynchus mykiss)
- Rice (Oryza sativa)
- Rice Blast Fungus (Magnaporthe grisea)
- Schistosome Parasite (Schistosoma mansoni)
- Sea Anemone (Nematostella vectensis)
- Sea Vase (Ciona intestinalis)
- Sea Urchin (Strongylocentrotus purpuratus)
- Sorghum (Sorghum bicolor)
- Soybean (Glycine max)
- Stem Rust Fungus (Puccinia graminis)
- Sugarcane (Saccharum officinarum)
- Thale Cress (Arabidopsis thaliana)
- Tomato (Lycopersicon esculentum)
- Toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- Wheat (Triticum aestivum)
- Worm (Caenorhabditis elegans)
Evolution for NOS2 Gene
- ENSEMBL:
- Gene Tree for NOS2 (if available)
- TreeFam:
- Gene Tree for NOS2 (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for NOS2: view image
Paralogs for NOS2 Gene
Pseudogenes.org Pseudogenes for NOS2 Gene
- PGOHUM00000292259
- PGOHUM00000292262
- PGOHUM00000292556
- PGOHUM00000292559
- PGOHUM00000293978
- PGOHUM00000294027
- PGOHUM00000294032
- PGOHUM00000294076
- PGOHUM00000294082
- PGOHUM00000294094
- PGOHUM00000294100
- PGOHUM00000294179
- PGOHUM00000294283
- PGOHUM00000294320
- PGOHUM00000294404
- PGOHUM00000294410
- PGOHUM00000294416
- PGOHUM00000297417
- PGOHUM00000298122
- PGOHUM00000288138
- PGOHUM00000288146
- PGOHUM00000288150
- PGOHUM00000288252
- PGOHUM00000288259
- PGOHUM00000288271
- PGOHUM00000288283
- PGOHUM00000288289
Variants for NOS2 Gene
Polymorphic Variants from UniProtKB/Swiss-Prot for NOS2 Gene
- NOS2_HUMAN-P35228
- Genetic variations in NOS2 are involved in resistance to malaria [MIM:611162].
Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NOS2 Gene
| SNP ID | Clinical significance and condition | Chr 17 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| 14013 | Protective: Malaria, resistance to | 27,801,702(-) | G/A | ||
| 709950 | Likely Benign: not provided | 27,765,618(-) | G/A | MISSENSE_VARIANT | |
| 710107 | Benign: not provided | 27,758,905(-) | G/A | SYNONYMOUS_VARIANT | |
| 710108 | Benign: not provided | 27,772,298(-) | G/A | INTRON_VARIANT | |
| 712632 | Likely Benign: not provided | 27,778,981(-) | G/A | SYNONYMOUS_VARIANT |
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| esv2715781 | CNV | deletion | 23290073 |
| esv2758683 | CNV | gain | 17122850 |
| esv28579 | CNV | gain | 19812545 |
| esv3640299 | OTHER | inversion | 21293372 |
| nsv471953 | CNV | novel sequence insertion | 20440878 |
| nsv9533 | CNV | gain | 18304495 |
| nsv960452 | CNV | duplication | 23825009 |
| nsv962299 | CNV | duplication | 23825009 |
Residual Variation Intolerance Score:
17.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
9.85;
89.64% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for NOS2 Gene
SNP Genotyping and Copy Number Assay Products
Disorders for NOS2 Gene
(96) MalaCards diseases for NOS2 Gene - From: LncRNADisease, OMIM, ClinVar, GTR, DISEASES, Novoseek, and GeneCards
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| malaria |
|
|
| meningioma, radiation-induced |
|
|
| glioma susceptibility 1 |
|
|
| borderline leprosy |
|
|
| chlamydia |
|
|
Search NOS2 in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for NOS2
- Genetic Association Database
- (GAD)
- Human Genome Epidemiology Navigator
- (HuGE)
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
- Open Targets Platform
No data available for UniProtKB/Swiss-Prot and Genatlas for NOS2 Gene
Publications for NOS2 Gene
- Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. (PMID: 7682706) Geller DA … Billiar TR (Proceedings of the National Academy of Sciences of the United States of America 1993) 2 3 4 23
- Genetic polymorphisms modifying oxidative stress are associated with disease activity in rheumatoid arthritis patients. (PMID: 19242068) Bohanec Grabar P … Dolzan V (Disease markers 2009) 3 23 41
- Influence of nitric oxide synthase gene polymorphisms on the risk of cardiovascular events in rheumatoid arthritis. (PMID: 19327239) Gonzalez-Gay MA … Martin J (Clinical and experimental rheumatology 2009) 3 23 41
- A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children. (PMID: 12433515) Hobbs MR … Weinberg JB (Lancet (London, England) 2002) 3 4 41
- Crystal structures of zinc-free and -bound heme domain of human inducible nitric-oxide synthase. Implications for dimer stability and comparison with endothelial nitric-oxide synthase. (PMID: 10409685) Li H … Poulos TL (The Journal of biological chemistry 1999) 3 4 23
ExternalLinks
Products for NOS2 Gene
- Custom Antibody ServicesOriGene Antibodies for NOS2
- Browse OriGene ELISA Kits
- Custom Assay Services
- Search Origene for MassSpec and Protein Over-expression Lysates for NOS2
- Origene Custom Protein Services for NOS2
- Origene shrna, sirna, and RNAi products in human, mouse, rat for NOS2
- Browse OriGene Inhibitory RNA Products For NOS2
- OriGene qPCR primer pairs for NOS2
- OriGene CRISPR knockouts for NOS2
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For NOS2
- Sino Biological Human cDNA Clone for NOS2
- Browse Sino Biological Recombinant Proteins
- Browse Sino Biological Cell Lysates
- Sino Biological Antibodies for NOS2
- Browse Sino Biological Assays
- Browse Antibodies
- Browse cDNA Clones
- Browse ELISA Kits
- Browse CRO Services
- High-throughput Antibody Production Service
- Protein Production Service
- Epitope Tag Antibodies & Reporter Protein Antibodies
- Lentiviral ORF Clones
- Novus Biologicals Antibodies for NOS2
- Novus Biologicals proteins and lysates for NOS2
- Novus Biologicals ELISA Kits for NOS2
- Browse Antibodies
- Browse Peptides and Proteins
- Browse Lysates
- Browse ELISA Kits
- Abcam antibodies for NOS2
- Abcam proteins for NOS2
- Find your target
- Browse Primary Antibodies
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- Browse ELISA Kits
- Browse Matched Antibody Pairs
- Browse Proteins and Peptides
- Search Knockout (KO) Validated Antibodies
- Browse Monoclonal Antibodies
- Browse Recombinant Antibodies
- Taconic Biosciences Mouse Models for NOS2
- Find your next knockout model in the Taconic Knockout Repository
- Genetically Engineered Models Available Immediately
- Humanized Mice
- Invitrogen Antibodies for NOS2 (AFLGC-NOS2)
- ApexBio compounds for NOS2
- VectorBuilder Custom and pre-made DNA vectors for NOS2 (ie. gene expression, shRNA knockdown, CRISPR)
- VectorBuilder Viral vectors for NOS2 (ie. lentivirus, AAV, adenovirus)
- VectorBuilder CRISPR vectors for NOS2 (ie. knockout, knockin, CRISPRa, CRISPRi)
- VectorBuilder Virus packaging for NOS2 CRISPR vectors (ie. lentivirus, AAV, adenovirus)
- VectorBuilder Stable cell line generation for NOS2
- Addgene plasmids for NOS2
- antibodies-online: Show 108 available NOS2 Antibodies ranked by validation data
- Compare Top NOS2 Antibodies
- Recommended
- 203 WB Antibodies (anti-human)
- 19 IHC (p) Antibodies (anti-human)
- 15 IHC Antibodies (anti-human)
- 12 IF (p) Antibodies (anti-human)
- 4 IP Antibodies (anti-human)
- 3 FACS Antibodies (anti-human)
- 1 IF Antibody (anti-human)
- 121 WB Antibodies (anti-mouse)
- 13 IHC Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 11 IHC (p) Antibodies (anti-mouse)
- 3 FACS Antibodies (anti-mouse)
- 71 WB Antibodies (anti-rat)
- 12 IF (p) Antibodies (anti-rat)
- 8 IHC Antibodies (anti-rat)
- 8 IHC (p) Antibodies (anti-rat)
- 4 IP Antibodies (anti-rat)
- antibodies-online: Show 101 available NOS2 Elisa Kits ranked by validation data
- Compare Top NOS2 Elisa Kits
- Quality Products:
- ABIN6730909
- ABIN1115495
- ABIN5526682
- ABIN415084
- ABIN365871
- ABIN1571522
- ABIN4947903
- ABIN4947902
- ABIN5522466
- ABIN955952
- ABIN6574224
- ABIN415396
- ABIN1115494
- ABIN366306
- ABIN455420
- ABIN1571523
- ABIN5522467
- ABIN5670001
- ABIN5593187
- ABIN6574225
- ABIN416102
- ABIN1115493
- ABIN367490
- ABIN579874
- ABIN1571524
- ABIN5522469
- ABIN5670665
- ABIN5593188
- ABIN431312
- ABIN368550
- ABIN1571525
- ABIN5522468
- ABIN1564704
- ABIN775151
- ABIN5657404
- ABIN2949938
- ABIN2537260
- ABIN6211012
- antibodies-online: Show 25 available NOS2 Proteins ranked by validation data
- Compare Top NOS2 Proteins
- Santa Cruz Biotechnology (SCBT) Antibodies for NOS2
- Search Santa Cruz Biotechnology (SCBT) for NOS2 siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for NOS2
- Horizon Cell Lines for NOS2
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NOS2
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NOS2
- Search GeneCopoeia for miRNA 3'UTR Target clone products for NOS2
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NOS2
Sources for NOS2 Gene
- (1) GeneCards
- (2) HGNC
- (3) NCBI Entrez Gene
- (4) UniProtKB/Swiss-Prot
- (5) Ensembl
- (6) OMIM
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) Cell Signaling Technology
- (12) GO
- (13) UniProtKB/TrEMBL
- (14) InterPro
- (15) ProtoNet
- (16) Blocks
- (17) MGI
- (18) IUBMB
- (19) KEGG
- (20) MINT
- (21) STRING
- (22) IntAct
- (23) Novoseek
- (24) PharmGKB
- (25) DrugBank
- (26) HMDB
- (27) ASD
- (28) SAGE
- (29) SOURCE
- (30) HomoloGene
- (31) PanEnsembl
- (32) euGenes
- (33) SGD
- (34) FlyBase
- (35) WormBase
- (36) Pseudogene
- (37) DGV
- (38) dbSNP
- (39) GenAtlas
- (40) HGMD
- (41) GAD
- (42) BGMUT
- (43) HuGE
- (44) Atlas
- (45) GenBank
- (46) HORDE
- (47) HUGE
- (48) IMGT
- (49) Leiden
- (50) miRBase
- (51) DME
- (52) OriGene
- (53) PubMed
- (54) R&D Systems
- (55) TGDB
- (56) Tocris
- (57) Abcam
- (58) Novus Biologicals
- (59) ProSpec
- (60) Sino Biological
- (61) GenScript
- (62) Qiagen
- (63) OCA
- (64) Proteopedia
- (65) MOPED
- (66) neXtProt
- (67) Reactome
- (68) GeneGo (Thomson Reuters)
- (69) DISEASES
- (70) SIMAP
- (71) GenomeRNAi
- (72) LifeMap
- (73) miRTarBase
- (74) MalaCards
- (75) Invitrogen
- (76) BitterDB
- (77) ESI-BIO
- (78) RefSeq
- (79) BioSystems
- (80) MaxQB
- (81) IUPHAR
- (82) BioGPS
- (83) Illumina
- (84) COMPARTMENTS
- (85) HOMER
- (86) PaxDb
- (87) ApexBio
- (88) Addgene
- (89) antibodies-online
- (90) CYP
- (91) NONCODE
- (92) TreeFam
- (93) PathCards
- (94) GeneReviews
- (95) GeneTex
- (96) Taconic Biosciences
- (97) GTEx
- (98) ProteomicsDB
- (99) SCBT
- (100) DGIdb
- (101) ClinicalTrials
- (102) RVIS
- (103) SIGNOR
- (104) diseasecard
- (105) NIH Rare Diseases
- (106) Orphanet
- (107) UMLS
- (108) GTR
- (109) Disease Ontology
- (110) Genetics Home Reference
- (111) MeSH
- (112) MedlinePlus
- (113) CDC
- (114) NINDS
- (115) NCBI Bookshelf
- (116) ClinVar
- (117) Gene Damage Index
- (118) HPO
- (119) UDN
- (120) VISTA
- (121) FANTOM5
- (122) ENCODE
- (123) ProSci
- (124) Horizon
- (125) The Signaling Pathways Project (SPP)
- (126) IID
- (127) VectorBuilder
- (128) SNPedia
- (129) BRCA Exchange
- (130) CIViC
- (131) ProteoGenix
- (132) dbSUPER
- (133) TISSUES
- (134) Gene ORGANizer
- (135) abm
- (136) Human Protein Atlas
- (137) GWAS Catalog
- (138) Monarch Initiative
- (139) DataMed
- (140) HumanCyc
- (141) UCNEbase
- (142) EPDnew
- (143) Applied Biosystems
- (144) GlyConnect
- (145) Aminode
- (146) GeneCopoeia
- (147) GPS-Prot
- (148) RNACentral
- (149) LncRNADisease
- (150) LOVD
- (151) miR2Disease
- (152) DiseaseEnhancer
- (153) Boster Bio
- (154) Biorbyt
- (155) Biocompare
- (156) Mastermind
- (157) CTD^2 Dashboard
- (158) CraniofacialAtlas
- (159) GeneImprint
- (160) Synthego
- (161) ENA
- (162) GtRNAdb
- (163) LncBase
- (164) LncBook
- (165) LNCipedia
- (166) Modomics
- (167) PDBe
- (168) Rfam
- (169) SILVA
- (170) snOPY
- (171) SRPDB
- (172) GENCODE
- (173) TarBase
- (174) Signalway Antibody
- (175) Open Targets Platform
- (176) 5SRRNADB
- (177) snoDB
- (178) MirGeneDB