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Aliases for NLK Gene
Aliases for NLK Gene
External Ids for NLK Gene
- HGNC: 29858
- Entrez Gene: 51701
- Ensembl: ENSG00000087095
- OMIM: 609476
- UniProtKB: Q9UBE8
Previous GeneCards Identifiers for NLK Gene
- GC17P026225
- GC17P028269
- GC17P026515
- GC17P026514
- GC17P023393
- GC17P026369
- GC17P022577
Summaries for NLK Gene
GeneCards Summary for NLK Gene
NLK (Nemo Like Kinase) is a Protein Coding gene. Among its related pathways are Wnt Signaling Pathway and Pluripotency and Non-Canonical Wnt Pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAPK7.
UniProtKB/Swiss-Prot Summary for NLK Gene
-
Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
Additional gene information for NLK Gene
- Monarch Initiative
- Search for NLK at DataMed
- Search for NLK at HumanCyc
No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NLK Gene
Genomics for NLK Gene
GeneHancer (GH) Regulatory Elements for NLK Gene
Promoters and enhancers for NLK Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around NLK on UCSC Golden Path with GeneCards custom track
- Top Transcription factor binding sites by QIAGEN in the NLK gene promoter:
-
- E2F
- E2F-2
- E2F-3a
- E2F-4
- E2F-5
- p300
- PPAR-gamma1
- PPAR-gamma2
Genomic Locations for NLK Gene
Genomic Locations for NLK Gene
- chr17:28,041,737-28,205,140
- (GRCh38/hg38)
- Size:
- 163,404 bases
- Orientation:
- Plus strand
- chr17:26,368,763-26,523,407
- (GRCh37/hg19)
- Size:
- 154,645 bases
- Orientation:
- Plus strand
Genomic View for NLK Gene
Genes around NLK on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 17q11.2 by HGNC
- 17q11.2 by Entrez Gene
- 17q11.2 by Ensembl
NLK Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for NLK Gene
Proteins for NLK Gene
-
Protein details for NLK Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- Q9UBE8-NLK_HUMAN
- Recommended name:
- Serine/threonine-protein kinase NLK
- Protein Accession:
- Q9UBE8
- B2RCX1
- Q2PNI9
- Q6P2A3
Protein attributes for NLK Gene
- Size:
- 527 amino acids
- Molecular mass:
- 58283 Da
- Cofactor:
- Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Quaternary structure:
-
- Homodimer. Homodimerization is required for intermolecular autophosphorylation, kinase activation and nuclear localization (By similarity). May interact with components of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes (By similarity). Interacts with LEF1, MEF2A, MYBL1 and MYBL2 (By similarity). Interacts with the upstream activating kinases HIPK2 and MAP3K7/TAK1. Interaction with MAP3K7/TAK1 seems to be indirect, and may be mediated by other proteins such as STAT3, TAB1 and TAB2. Interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO3, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4. Interacts with DAPK3/ZIPK, and this interaction may disrupt interaction with transcription factors such as TCF7L2/TCF4. Forms a transcriptional repressor complex with CHD7, PPARG and SETDB1. Interacts with RNF138/NARF. Interacts with ATF5; the interaction stabilizes ATF5 at the protein level in a kinase-independent manner (PubMed:25512613).
- SequenceCaution:
-
- Sequence=AAD56013.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAF04857.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=ABC40748.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAG37718.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Protein Expression for NLK Gene
Selected DME Specific Peptides for NLK Gene
- Q9UBE8:
-
- TDLLGTP
- GKRVALKKMPNVFQNL
- WSVGCIFAE
- KPGNLLVN
- WSVGCIFAELLGR
- LRGLKYLHSA
- YQILRGLKY
- LKICDFG
- PDRPIGYGAFGVVWSVTDPR
- SCKRVFRE
- DIWSVGCI
- FDPSKRI
- HRDIKPGN
- LKICDFGLAR
Post-translational modifications for NLK Gene
- Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.
- Modification sites at PhosphoSitePlus
- Modification sites at neXtProt
Other Protein References for NLK Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- G Biosciences Immunotag™ NLK Antibody (ITA7602-100u) and Immunotag™ NLK Antibody (ITA7305-100u)
- Atlas Antibodies Anti-NLK Antibody (HPA018192) and Anti-NLK Antibody (HPA056511)
- GeneTex Anti-NLK antibody [N3C3] and Anti-NLK antibody
-
Custom Antibody ServicesOriGene Antibodies for NLK
- Novus Biologicals Antibodies for NLK
-
Abcam antibodies for NLK
- Invitrogen Antibodies for NLK (AFLGC-NLK)
- Sino Biological Antibodies for NLK
Protein Products
- Atlas Antibodies PrEST Antigen NLK (APrEST73478) and PrEST Antigen NLK (APrEST91733)
- Creative Diagnostics Human NLK peptide
- Search Origene for MassSpec and Protein Over-expression Lysates for NLK
- Origene Custom Protein Services for NLK
- Sino Biological Recombinant Proteins for NLK
- Sino Biological Cell Lysates for NLK
- antibodies-online: Show 11 available NLK Proteins ranked by validation data
- Compare Top NLK Proteins
-
Abcam proteins for NLK
Assay Products
- antibodies-online: Show 8 available NLK Elisa Kits ranked by validation data
- Compare Top NLK Elisa Kits
-
Quality Products:
Domains & Families for NLK Gene
Gene Families for NLK Gene
- IUPHAR :
- Human Protein Atlas (HPA):
-
- Enzymes
- Predicted intracellular proteins
Protein Domains for NLK Gene
- Blocks:
- InterPro:
- ProtoNet:
Suggested Antigen Peptide Sequences for NLK Gene
- GenScript: Design optimal peptide antigens:
-
- Protein LAK1 (NLK_HUMAN)
Graphical View of Domain Structure for InterPro Entry
Q9UBE8UniProtKB/Swiss-Prot:
NLK_HUMAN :- Contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. This mode of activation contrasts with that of classical MAP kinases, which contain a TXY activation loop motif in which phosphorylation of both the threonine and tyrosine residues is required for kinase activation.
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Domain:
-
- Contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. This mode of activation contrasts with that of classical MAP kinases, which contain a TXY activation loop motif in which phosphorylation of both the threonine and tyrosine residues is required for kinase activation.
- Family:
-
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Function for NLK Gene
Molecular function for NLK Gene
- UniProtKB/Swiss-Prot Function:
- Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.24;.
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.24;.
- UniProtKB/Swiss-Prot EnzymeRegulation:
- Activated by dimerization and subsequent intermolecular autophosphorylation on Thr-298 (By similarity). Activated by the non-canonical Wnt signaling pathway, in which WNT5A treatment leads to activation of MAP3K7/TAK1 and HIPK2, which subsequently phosphorylates and activates this protein. Other cytokines such as IL6 may also activate this regulatory circuit.
Enzyme Numbers (IUBMB) for NLK Gene
Phenotypes From GWAS Catalog for NLK Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000166 | nucleotide binding | IEA | -- |
| GO:0000287 | magnesium ion binding | ISS | -- |
| GO:0004672 | protein kinase activity | TAS,IEA | 10863097 |
| GO:0004674 | protein serine/threonine kinase activity | IEA,TAS | -- |
| GO:0004707 | MAP kinase activity | IEA,ISS | -- |
Phenotypes for NLK Gene
- MGI mutant phenotypes for NLK:
- inferred from 5 alleles
- GenomeRNAi human phenotypes for NLK:
-
- Condensed cis-Golgi
- Decreased viability ratio
- Decreased cell proliferation
- Decreased Hepatitis C virus replication
- Increased shRNA abundance (Z-score > 2)
- Decreased focal adhesion (FA) area, decreased FA length, decreased FA mean intensity, increased number of small and round FAs, increased FA abundance
- Decreased viability of wild-type and TP53 knockout cells, decreased TP53 protein expression ratio (wild-type / TP53 knockout cells)
- Downregulation of Wnt pathway after Wnt3A stimulation
- Decreased shRNA abundance (Z-score < -2)
- Decreased viability
- Increased cell viability after pRB stimulation
- no effect
- Increased vaccinia virus (VACV) infection
- Resistant to vaccinia virus (VACV-A4L) infection
- Apoptosis resistance
- Decreased melanin production
- Mildly decreased CFP-tsO45G cell surface transport
- Decreased viability after Maraba virus infection
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR Products
-
OriGene CRISPR knockouts for NLK
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NLK
- genomics-online: gRNA clones - Search results for available NLK gene related products
- Applied Biological Materials (abm): CRISPR Clones for NLK - Select products 50% OFF >
- * NLK CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * NLK CRISPR applications: KO, Activation, Repression, and more - Browse All
-
Santa Cruz Biotechnology (SCBT) CRISPR for NLK
miRNA for NLK Gene
- miRTarBase miRNAs that target NLK
-
- hsa-mir-181c-5p (MIRT000233)
- hsa-mir-181b-5p (MIRT000238)
- hsa-mir-181a-5p (MIRT000243)
- hsa-mir-215-5p (MIRT024563)
- hsa-mir-192-5p (MIRT026531)
- hsa-mir-92b-3p (MIRT035518)
- hsa-mir-23a-3p (MIRT050387)
- hsa-mir-199b-5p (MIRT054629)
- hsa-mir-101-3p (MIRT438193)
- hsa-mir-3682-5p (MIRT472135)
- hsa-mir-513a-5p (MIRT472136)
- hsa-mir-545-3p (MIRT472137)
- hsa-mir-3120-3p (MIRT472138)
- hsa-mir-5692a (MIRT472139)
- hsa-mir-27a-3p (MIRT472140)
- hsa-mir-27b-3p (MIRT472141)
- hsa-mir-190a-3p (MIRT522254)
- hsa-mir-5011-5p (MIRT522255)
- hsa-mir-1277-5p (MIRT522256)
- hsa-mir-3121-3p (MIRT522257)
- hsa-mir-548c-3p (MIRT522258)
- hsa-mir-574-5p (MIRT627953)
- hsa-mir-6818-5p (MIRT627954)
- hsa-mir-664b-3p (MIRT627955)
- hsa-mir-579-3p (MIRT627956)
- hsa-mir-5696 (MIRT627957)
- hsa-mir-6867-5p (MIRT627958)
- hsa-mir-1304-3p (MIRT627959)
- hsa-mir-153-5p (MIRT627960)
- hsa-mir-1250-3p (MIRT627961)
- hsa-mir-1285-5p (MIRT627962)
- hsa-mir-216a-5p (MIRT627963)
- hsa-mir-6802-5p (MIRT701392)
- hsa-mir-6800-5p (MIRT701393)
- hsa-mir-6822-3p (MIRT701394)
- hsa-mir-3622b-5p (MIRT701395)
- hsa-mir-6770-5p (MIRT701396)
- hsa-mir-1253 (MIRT701397)
- hsa-mir-6741-3p (MIRT701398)
- hsa-mir-135a-3p (MIRT701399)
- hsa-mir-6865-5p (MIRT701400)
- hsa-mir-6815-5p (MIRT701401)
- hsa-mir-3158-3p (MIRT701402)
- hsa-mir-4446-3p (MIRT701403)
- hsa-mir-5088-5p (MIRT701404)
- hsa-mir-944 (MIRT701405)
- hsa-mir-409-3p (MIRT733422)
miRNA Products
Inhibitory RNA Products
- Origene sirna, shrna, and RNAi products in human, mouse, rat for NLK
- Browse OriGene Inhibitory RNA Products For NLK
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NLK
- genomics-online: shRNA clones - Search results for available NLK gene related products
Clone Products
- Sino Biological Human cDNA Clone for NLK
- Addgene plasmids for NLK
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NLK
- genomics-online: cdna clones - Search results for available NLK gene related products
- orf clones - Search results for available NLK gene related products
- Applied Biological Materials (abm): Clones for NLK - Select products 50% OFF >
- * NLK as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * NLK tags and reporters available: His, HA, Myc, Flag, GFP, RFP, Luciferase - Browse All
Cell Line Products
-
Horizon Cell Lines for NLK
No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NLK Gene
Localization for NLK Gene
Subcellular locations from UniProtKB/Swiss-Prot for NLK Gene
- Nucleus. Cytoplasm. Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity). {ECO:0000250}.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytosol | 4 |
| extracellular | 1 |
| cytoskeleton | 1 |
| peroxisome | 1 |
- Cell Junctions (3)
- Nucleoli (3)
- Nucleus (3)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005634 | nucleus | ISS,TAS | -- |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005737 | cytoplasm | IBA | 21873635 |
| GO:0005829 | cytosol | TAS | -- |
Pathways & Interactions for NLK Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | MAPK signaling pathway | ||
| 2 | CDK-mediated phosphorylation and removal of Cdc6 |
.41
|
|
| 3 | Mesodermal Commitment Pathway | ||
| 4 | Wnt / Hedgehog / Notch | ||
| 5 | Adherens junction | ||
Pathways by source for NLK Gene
1 Sino Biological pathway for NLK Gene
10 BioSystems pathways for NLK Gene
4 Reactome pathways for NLK Gene
4 KEGG pathways for NLK Gene
1 R&D Systems pathway for NLK Gene
1 Qiagen pathway for NLK Gene
- Molecular Mechanisms of Cancer
1 Cell Signaling Technology pathway for NLK Gene
1 GeneTex pathway for NLK Gene
Interacting Proteins for NLK Gene
Selected Interacting proteins:
Q9UBE8-NLK_HUMAN
ENSP00000384625
for NLK Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for NLK
SIGNOR curated interactions for NLK Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000165 | MAPK cascade | IEA | -- |
| GO:0006355 | regulation of transcription, DNA-templated | ISS | -- |
| GO:0006468 | protein phosphorylation | IEA,ISS | -- |
| GO:0007179 | transforming growth factor beta receptor signaling pathway | IMP | 15004007 |
| GO:0007223 | Wnt signaling pathway, calcium modulating pathway | TAS | -- |
Drugs & Compounds for NLK Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Magnesium | Approved, Experimental, Investigational | Pharma | 0 |
Drug Products
- Atlas Antibodies PrEST Antigen NLK (APrEST73478) and PrEST Antigen NLK (APrEST91733)
- Creative Diagnostics Human NLK peptide
Transcripts for NLK Gene
mRNA/cDNA for NLK Gene
- (1) REFSEQ mRNAs :
- (12) Additional mRNA sequences :
- (185) Selected AceView cDNA sequences:
- (6) Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000583517 695 bps (ENST00000583517.1)
- ENST00000407008 3526 bps (ENST00000407008.7)
- ENST00000582037 1503 bps (ENST00000582037.2)
- ENST00000496808 1702 bps (ENST00000496808.1)
- ENST00000584188 451 bps (ENST00000584188.1)
CRISPR Products
-
OriGene CRISPR knockouts for NLK
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NLK
- genomics-online: gRNA clones - Search results for available NLK gene related products
- Applied Biological Materials (abm): CRISPR Clones for NLK - Select products 50% OFF >
- * NLK CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * NLK CRISPR applications: KO, Activation, Repression, and more - Browse All
-
Santa Cruz Biotechnology (SCBT) CRISPR for NLK
miRNA Products
Inhibitory RNA Products
- Origene sirna, shrna, and RNAi products in human, mouse, rat for NLK
- Browse OriGene Inhibitory RNA Products For NLK
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NLK
- genomics-online: shRNA clones - Search results for available NLK gene related products
Clone Products
- Sino Biological Human cDNA Clone for NLK
- Addgene plasmids for NLK
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NLK
- genomics-online: cdna clones - Search results for available NLK gene related products
- orf clones - Search results for available NLK gene related products
- Applied Biological Materials (abm): Clones for NLK - Select products 50% OFF >
- * NLK as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * NLK tags and reporters available: His, HA, Myc, Flag, GFP, RFP, Luciferase - Browse All
Expression for NLK Gene
mRNA differential expression in normal tissues according to GTEx for NLK Gene
This gene is overexpressed in Brain - Cortex (x5.0) and Brain - Frontal Cortex (BA9) (x5.0).
This gene is overexpressed in Brain (49.1) and Retina (19.9).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for NLK Gene
- Elite partner
NURSA nuclear receptor signaling pathways regulating expression of NLK Gene:
NLKSOURCE GeneReport for Unigene cluster for NLK Gene:
Hs.208759Evidence on tissue expression from TISSUES for NLK Gene
- Nervous system(4.6)
- Blood(4.3)
- Eye(2.2)
Primer Products
-
OriGene qPCR primer pairs for NLK
-
OriGene qPCR primer pairs and template standards for NLK
- genomics-online: primer clones - Search results for available NLK gene related products
Gene Expression Assay Products
No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for NLK Gene
Orthologs for NLK Gene
This gene was present in the common ancestor of animals.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| platypus (Ornithorhynchus anatinus) |
Mammalia | NLK 33 |
|
OneToOne | |
| chimpanzee (Pan troglodytes) |
Mammalia | NLK 33 32 |
|
OneToOne | |
| oppossum (Monodelphis domestica) |
Mammalia | NLK 33 |
|
OneToOne | |
| dog (Canis familiaris) |
Mammalia | NLK 33 32 |
|
OneToOne | |
| cow (Bos Taurus) |
Mammalia | NLK 33 32 |
|
OneToOne | |
| rat (Rattus norvegicus) |
Mammalia | Nlk 32 |
|
||
| mouse (Mus musculus) |
Mammalia | Nlk 17 33 32 |
|
||
| chicken (Gallus gallus) |
Aves | NLK 33 32 |
|
OneToOne | |
| lizard (Anolis carolinensis) |
Reptilia | NLK 33 |
|
OneToOne | |
| tropical clawed frog (Silurana tropicalis) |
Amphibia | nlk 32 |
|
||
| Str.7425 32 |
|
||||
| African clawed frog (Xenopus laevis) |
Amphibia | xnlk 32 |
|
||
| zebrafish (Danio rerio) |
Actinopterygii | nlk2 33 32 |
|
OneToOne | |
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP005898 32 |
|
||
| fruit fly (Drosophila melanogaster) |
Insecta | nmo 33 34 32 |
|
OneToOne | |
| worm (Caenorhabditis elegans) |
Secernentea | lit-1 33 32 |
|
OneToOne | |
| sea squirt (Ciona savignyi) |
Ascidiacea | CSA.3781 33 |
|
OneToOne |
- Species where no ortholog for NLK was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Ashbya gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- Alicante grape (Vitis vinifera)
- alpha proteobacteria (Wolbachia pipientis)
- amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- baker's yeast (Saccharomyces cerevisiae)
- barley (Hordeum vulgare)
- beta proteobacteria (Neisseria meningitidis)
- bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- common water flea (Daphnia pulex)
- corn (Zea mays)
- E. coli (Escherichia coli)
- filamentous fungi (Aspergillus nidulans)
- Firmicute bacteria (Streptococcus pneumoniae)
- fission yeast (Schizosaccharomyces pombe)
- green algae (Chlamydomonas reinhardtii)
- honey bee (Apis mellifera)
- K. lactis yeast (Kluyveromyces lactis)
- loblloly pine (Pinus taeda)
- malaria parasite (Plasmodium falciparum)
- medicago trunc (Medicago Truncatula)
- moss (Physcomitrella patens)
- orangutan (Pongo pygmaeus)
- pig (Sus scrofa)
- rainbow trout (Oncorhynchus mykiss)
- rice (Oryza sativa)
- rice blast fungus (Magnaporthe grisea)
- schistosome parasite (Schistosoma mansoni)
- sea anemone (Nematostella vectensis)
- sea urchin (Strongylocentrotus purpuratus)
- sorghum (Sorghum bicolor)
- soybean (Glycine max)
- stem rust fungus (Puccinia graminis)
- sugarcane (Saccharum officinarum)
- thale cress (Arabidopsis thaliana)
- tomato (Lycopersicon esculentum)
- toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- wheat (Triticum aestivum)
Evolution for NLK Gene
- ENSEMBL:
- Gene Tree for NLK (if available)
- TreeFam:
- Gene Tree for NLK (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for NLK: view image
Paralogs for NLK Gene
Paralogs for NLK Gene
(47) SIMAP similar genes for NLK Gene using alignment to 3 proteins:
- NLK_HUMAN
- H0YD75_HUMAN
- J3QSE9_HUMAN
Variants for NLK Gene
| SNP ID | Clin | Chr 17 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| VAR_042273 | A glioblastoma multiforme sample | p.Ala343Thr | |||
| rs1000018096 | -- | 28,146,449(+) | T/C | intron_variant | |
| rs1000039716 | -- | 28,099,639(+) | C/T | intron_variant | |
| rs1000051167 | -- | 28,163,458(+) | A/G | intron_variant | |
| rs1000060068 | -- | 28,125,300(+) | A/G | intron_variant |
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| esv2678164 | CNV | deletion | 23128226 |
| esv3353471 | CNV | insertion | 20981092 |
| nsv2016 | CNV | deletion | 18451855 |
| nsv522349 | CNV | gain | 19592680 |
Residual Variation Intolerance Score:
23.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
0.59;
12.76% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for NLK Gene
- SNPedia medical, phenotypic, and genealogical associations of SNPs for
- NLK
SNP Genotyping and Copy Number Assay Products
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NLK Gene
Disorders for NLK Gene
Additional Disease Information for NLK
- Genetic Association Database
- (GAD)
- Human Genome Epidemiology Navigator
- (HuGE)
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
No disorders were found for NLK Gene.
No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NLK Gene
Publications for NLK Gene
- Characterization of the Fugu rubripes NLK and FN5 genes flanking the NF1 (Neurofibromatosis type 1) gene in the 5' direction and mapping of the human counterparts. (PMID: 10863097) Kehrer-Sawatzki H … Krone W (Gene 2000) 2 3 4 23 56
- Regulation of FOXO1 by TAK1-Nemo-like kinase pathway. (PMID: 20061393) Kim S … Chung J (The Journal of biological chemistry 2010) 3 4 23 56
- STAT3 regulates Nemo-like kinase by mediating its interaction with IL-6-stimulated TGFbeta-activated kinase 1 for STAT3 Ser-727 phosphorylation. (PMID: 15764709) Kojima H … Nakajima K (Proceedings of the National Academy of Sciences of the United States of America 2005) 3 4 23 56
- Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK. (PMID: 15082531) Kanei-Ishii C … Ishii S (Genes & development 2004) 3 4 23 56
- Stabilization of ATF5 by TAK1-Nemo-like kinase critically regulates the interleukin-1β-stimulated C/EBP signaling pathway. (PMID: 25512613) Zhang ZY … Zhang XD (Molecular and cellular biology 2015) 3 4 56
ExternalLinks
Products for NLK Gene
- Custom Antibody ServicesOriGene Antibodies for NLK
- Browse OriGene ELISA Kits
- Custom Assay Services
- Search Origene for MassSpec and Protein Over-expression Lysates for NLK
- Origene Custom Protein Services for NLK
- Origene sirna, shrna, and RNAi products in human, mouse, rat for NLK
- Browse OriGene Inhibitory RNA Products For NLK
- OriGene qPCR primer pairs for NLK
- OriGene qPCR primer pairs and template standards for NLK
- OriGene CRISPR knockouts for NLK
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For NLK
- Sino Biological Human cDNA Clone for NLK
- Sino Biological Recombinant Proteins for NLK
- Sino Biological Cell Lysates for NLK
- Sino Biological Antibodies for NLK
- Browse Sino Biological Assays
- Browse Recombinant Proteins
- Browse Antibodies
- Browse cDNA Clones
- Browse ELISA Kits
- Browse CRO Services
- High-throughput Antibody Production Service
- Protein Production Service
- Epitope Tag Antibodies & Reporter Protein Antibodies
- Lentiviral ORF Clones
- Novus Biologicals Antibodies for NLK
- Novus Biologicals proteins and lysates for NLK
- Browse Antibodies
- Browse Peptides and Proteins
- Browse Lysates
- Browse ELISA Kits
- Abcam antibodies for NLK
- Abcam proteins for NLK
- Find your target
- Browse Primary Antibodies
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- Browse ELISA Kits
- Browse Matched Antibody Pairs
- Browse Proteins and Peptides
- Search Knockout (KO) Validated Antibodies
- Browse Monoclonal Antibodies
- Browse Recombinant Antibodies
- Invitrogen Antibodies for NLK (AFLGC-NLK)
- Addgene plasmids for NLK
- antibodies-online: Show 109 available NLK Antibodies ranked by validation data
- Compare Top NLK Antibodies
- antibodies-online: Show 8 available NLK Elisa Kits ranked by validation data
- Compare Top NLK Elisa Kits
- Quality Products:
- antibodies-online: Show 11 available NLK Proteins ranked by validation data
- Compare Top NLK Proteins
- Search Santa Cruz Biotechnology (SCBT) for NLK siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for NLK
- Horizon Cell Lines for NLK
- genomics-online: cdna clones - Search results for available NLK gene related products
- orf clones - Search results for available NLK gene related products
- genomics-online: gRNA clones - Search results for available NLK gene related products
- genomics-online: primer clones - Search results for available NLK gene related products
- genomics-online: shRNA clones - Search results for available NLK gene related products
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for NLK
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for NLK
- Search GeneCopoeia for miRNA 3'UTR Target clone products for NLK
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NLK
Sources for NLK Gene
- (1) GeneCards
- (2) HGNC
- (3) NCBI Entrez Gene
- (4) UniProtKB/Swiss-Prot
- (5) Ensembl
- (6) OMIM
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) Cell Signaling Technology
- (12) GO
- (13) UniProtKB/TrEMBL
- (14) InterPro
- (15) ProtoNet
- (16) Blocks
- (17) MGI
- (18) IUBMB
- (19) KEGG
- (20) MINT
- (21) STRING
- (22) IntAct
- (23) Novoseek
- (24) PharmGKB
- (25) DrugBank
- (26) HMDB
- (27) AceView
- (28) ASD
- (29) ECgene
- (30) CGAP SAGE
- (31) SOURCE
- (32) HomoloGene
- (33) PanEnsembl
- (34) euGenes
- (35) SGD
- (36) FlyBase
- (37) WormBase
- (38) Pseudogene
- (39) DGV
- (40) dbSNP
- (41) GenAtlas
- (42) HGMD
- (43) GAD
- (44) BGMUT
- (45) HuGE
- (46) Atlas
- (47) GenBank
- (48) H-invDB
- (49) HORDE
- (50) HUGE
- (51) IMGT
- (52) Leiden
- (53) miRBase
- (54) DME
- (55) OriGene
- (56) PubMed
- (57) R&D Systems
- (58) TGDB
- (59) Tocris
- (60) Abcam
- (61) Novus Biologicals
- (62) ProSpec
- (63) Sino Biological
- (64) GenScript
- (65) Qiagen
- (66) OCA
- (67) Proteopedia
- (68) MOPED
- (69) neXtProt
- (70) Reactome
- (71) GeneGo (Thomson Reuters)
- (72) DISEASES
- (73) SIMAP
- (74) GenomeRNAi
- (75) LifeMap
- (76) miRTarBase
- (77) MalaCards
- (78) Invitrogen
- (79) BitterDB
- (80) ESI-BIO
- (81) RefSeq
- (82) BioSystems
- (83) MaxQB
- (84) IUPHAR
- (85) BioGPS
- (86) Illumina
- (87) COMPARTMENTS
- (88) HOMER
- (89) PaxDb
- (90) ApexBio
- (91) Addgene
- (92) antibodies-online
- (93) CYP
- (94) NONCODE
- (95) TreeFam
- (96) PathCards
- (97) GeneReviews
- (98) GeneTex
- (99) Taconic Biosciences
- (100) GTEx
- (101) ProteomicsDB
- (102) SCBT
- (103) DGIdb
- (104) ClinicalTrials
- (105) RVIS
- (106) SIGNOR
- (107) diseasecard
- (108) NIH Rare Diseases
- (109) Orphanet
- (110) UMLS
- (111) GTR
- (112) Disease Ontology
- (113) Genetics Home Reference
- (114) MeSH
- (115) MedlinePlus
- (116) CDC
- (117) NINDS
- (118) NCBI Bookshelf
- (119) ClinVar
- (120) Gene Damage Index
- (121) HPO
- (122) UDN
- (123) VISTA
- (124) FANTOM5
- (125) ENCODE
- (126) ProSci
- (127) Horizon
- (128) NURSA
- (129) IID
- (130) VectorBuilder
- (131) SNPedia
- (132) BRCA Exchange
- (133) CIViC
- (134) ProteoGenix
- (135) dbSUPER
- (136) TISSUES
- (137) Gene ORGANizer
- (138) abm
- (139) Human Protein Atlas
- (140) GWAS Catalog
- (141) Monarch Initiative
- (142) DataMed
- (143) HumanCyc
- (144) genomics-online
- (145) UCNEbase
- (146) EPDnew
- (147) Applied Biosystems
- (148) GlyConnect
- (149) Aminode
- (150) GeneCopoeia
- (151) GPS-Prot
- (152) RNACentral
- (153) LncRNADisease
- (154) LOVD
- (155) miR2Disease
- (156) DiseaseEnhancer
- (157) Boster Bio
- (158) Biorbyt
- (159) Biocompare
- (160) Mastermind
- (161) CTD^2 Dashboard
- (162) CraniofacialAtlas
- (163) GeneImprint
- (164) Synthego
- (165) Bio Basic
- (166) ENA
- (167) GtRNAdb
- (168) LncBase
- (169) LncBook
- (170) LNCipedia
- (171) LncRNAdb
- (172) Modomics
- (173) PDBe
- (174) Rfam
- (175) SILVA
- (176) snOPY
- (177) SRPDB
- (178) GENCODE
- (179) TarBase
- (180) MyBioSource