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Aliases for NIT1 Gene

Aliases for NIT1 Gene

  • Nitrilase 1 2 3 5
  • Nitrilase Homolog 1 4
  • DGSH Amidase 4
  • EC 3.5.1.- 4

External Ids for NIT1 Gene

Previous GeneCards Identifiers for NIT1 Gene

  • GC01P158799
  • GC01P156820
  • GC01P157865
  • GC01P158304
  • GC01P157900
  • GC01P159355
  • GC01P161087
  • GC01P132445

Summaries for NIT1 Gene

Entrez Gene Summary for NIT1 Gene

  • This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

GeneCards Summary for NIT1 Gene

NIT1 (Nitrilase 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds and nitrilase activity.

UniProtKB/Swiss-Prot for NIT1 Gene

  • Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.

Additional gene information for NIT1 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NIT1 Gene

Genomics for NIT1 Gene

GeneHancer (GH) Regulatory Elements for NIT1 Gene

Promoters and enhancers for NIT1 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01I161113 Promoter/Enhancer 2.5 EPDnew Ensembl ENCODE dbSUPER 560 +0.2 193 8.6 HDGF PKNOX1 CLOCK SMAD1 ARNT ARID4B SIN3A FEZF1 DMAP1 ZNF2 NIT1 PIR38070 PFDN2 RPSAP18 DUSP12 B4GALT3 OLFML2B F11R HSPA6 PIR51486
GH01I161196 Promoter/Enhancer 2.6 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 7.2 +82.0 81998 8 HDGF PKNOX1 SMAD1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 NDUFS2 ADAMTS4 DUSP12 B4GALT3 USP21 PPOX LOC100287049 DCAF8 NIT1 TOMM40L
GH01I161175 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 7 +58.8 58818 3.2 HDGF PKNOX1 SMAD1 FOXA2 MLX ARNT ZFP64 ARID4B SIN3A FEZF1 B4GALT3 USP21 LOC100287049 DUSP12 NIT1 PPOX
GH01I161158 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 7.9 +42.4 42426 4.9 HDGF SIN3A YY1 POLR2B E2F8 ZNF207 ZNF143 DEK SP3 SP5 USP21 ENSG00000224985 NIT1 TSTD1 PPOX UFC1
GH01I161096 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 5.5 -20.4 -20394 2.9 YBX1 FEZF1 IRF4 YY1 ZNF213 E2F8 ZNF416 ZNF143 ZNF548 MCM3 KLHDC9 USP21 DUSP12 PPOX LOC100287049 DEDD NCSTN RPSAP18 LOC148430 DCAF8
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around NIT1 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the NIT1 gene promoter:

Genomic Locations for NIT1 Gene

Genomic Locations for NIT1 Gene
chr1:161,118,072-161,125,445
(GRCh38/hg38)
Size:
7,374 bases
Orientation:
Plus strand
chr1:161,087,862-161,095,235
(GRCh37/hg19)

Genomic View for NIT1 Gene

Genes around NIT1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NIT1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NIT1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NIT1 Gene

Proteins for NIT1 Gene

  • Protein details for NIT1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86X76-NIT1_HUMAN
    Recommended name:
    Deaminated glutathione amidase
    Protein Accession:
    Q86X76
    Secondary Accessions:
    • B1AQP3
    • D3DVF4
    • O76091

    Protein attributes for NIT1 Gene

    Size:
    327 amino acids
    Molecular mass:
    35896 Da
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • According to Rosetta Stone theory, the existence of a fusion protein in one genome predicts that the separate polypeptides expressed in other organisms function in the same cellular or biochemical pathway. In Drosophila melanogaster and Caenorhabditis elegans, NitFhit is a fusion protein composed of a C-terminal Fhit domain and a domain related to plant and bacterial nitrilase.

    Alternative splice isoforms for NIT1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NIT1 Gene

Post-translational modifications for NIT1 Gene

No Post-translational modifications

No data available for DME Specific Peptides for NIT1 Gene

Domains & Families for NIT1 Gene

Gene Families for NIT1 Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for NIT1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q86X76

UniProtKB/Swiss-Prot:

NIT1_HUMAN :
  • Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
Family:
  • Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
genes like me logo Genes that share domains with NIT1: view

Function for NIT1 Gene

Molecular function for NIT1 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
N-(4-oxoglutarate)-L-cysteinylglycine + H(2)O = 2-oxoglutarate + L-cysteinylglycine.
UniProtKB/Swiss-Prot Function:
Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.

Enzyme Numbers (IUBMB) for NIT1 Gene

Phenotypes From GWAS Catalog for NIT1 Gene

Gene Ontology (GO) - Molecular Function for NIT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000257 nitrilase activity TAS 9671749
GO:0016787 hydrolase activity IEA --
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds IEA --
genes like me logo Genes that share ontologies with NIT1: view
genes like me logo Genes that share phenotypes with NIT1: view

Animal Models for NIT1 Gene

MGI Knock Outs for NIT1:

Animal Model Products

CRISPR Products

miRNA for NIT1 Gene

miRTarBase miRNAs that target NIT1

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NIT1 Gene

Localization for NIT1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NIT1 Gene

Isoform 2: Mitochondrion.
Isoform 1: Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NIT1 gene
Compartment Confidence
extracellular 5
mitochondrion 5
nucleus 5
cytosol 4
plasma membrane 1
endoplasmic reticulum 1
lysosome 1

Gene Ontology (GO) - Cellular Components for NIT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA,HDA 21630459
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IEA --
GO:0070062 extracellular exosome HDA,IDA 23376485
genes like me logo Genes that share ontologies with NIT1: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for NIT1 Gene

Pathways & Interactions for NIT1 Gene

No Data Available

Gene Ontology (GO) - Biological Process for NIT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006807 nitrogen compound metabolic process IEA --
GO:0008150 biological_process ND --
genes like me logo Genes that share ontologies with NIT1: view

No data available for Pathways by source and SIGNOR curated interactions for NIT1 Gene

Drugs & Compounds for NIT1 Gene

No Compound Related Data Available

Transcripts for NIT1 Gene

Unigene Clusters for NIT1 Gene

Nitrilase 1:
Representative Sequences:

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NIT1 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b · 6c · 6d ^ 7a · 7b ^ 8a · 8b
SP1: - - - - - -
SP2: - - - -
SP3: - - - - - - -
SP4: -
SP5: - - - - - - - -
SP6: - - -
SP7: - - - - -
SP8: - - - - - - - -
SP9: - -
SP10:

Relevant External Links for NIT1 Gene

GeneLoc Exon Structure for
NIT1
ECgene alternative splicing isoforms for
NIT1

Expression for NIT1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NIT1 Gene

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NIT1 Gene



Protein tissue co-expression partners for NIT1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NIT1 Gene:

NIT1

SOURCE GeneReport for Unigene cluster for NIT1 Gene:

Hs.146406

mRNA Expression by UniProt/SwissProt for NIT1 Gene:

Q86X76-NIT1_HUMAN
Tissue specificity: Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.

Evidence on tissue expression from TISSUES for NIT1 Gene

  • Nervous system(4.7)
  • Liver(4.5)
  • Pancreas(3.2)
genes like me logo Genes that share expression patterns with NIT1: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NIT1 Gene

Orthologs for NIT1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NIT1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NIT1 33 34
  • 99.39 (n)
dog
(Canis familiaris)
Mammalia NIT1 33 34
  • 91.95 (n)
cow
(Bos Taurus)
Mammalia NIT1 33 34
  • 91.85 (n)
rat
(Rattus norvegicus)
Mammalia Nit1 33
  • 87.58 (n)
mouse
(Mus musculus)
Mammalia Nit1 33 16 34
  • 87.1 (n)
oppossum
(Monodelphis domestica)
Mammalia NIT1 34
  • 72 (a)
OneToOne
chicken
(Gallus gallus)
Aves NIT1 33 34
  • 66.34 (n)
tropical clawed frog
(Silurana tropicalis)
Amphibia nit1 33
  • 64.53 (n)
African clawed frog
(Xenopus laevis)
Amphibia Nit1 33
zebrafish
(Danio rerio)
Actinopterygii nit1 33 34
  • 58.07 (n)
Dr.16022 33
fruit fly
(Drosophila melanogaster)
Insecta NitFhit 35 34
  • 48 (a)
worm
(Caenorhabditis elegans)
Secernentea nft-1 35 34
  • 50 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NIT2 33 34 36
  • 47.17 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E15247g 33
  • 44.89 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G08790 33
  • 52.75 (n)
rice
(Oryza sativa)
Liliopsida Os12g0502500 33
  • 51.72 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPBC651.02 33
  • 50.75 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU05757 33
  • 50.18 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 47 (a)
OneToOne
Species where no ortholog for NIT1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NIT1 Gene

ENSEMBL:
Gene Tree for NIT1 (if available)
TreeFam:
Gene Tree for NIT1 (if available)

Paralogs for NIT1 Gene

(1) SIMAP similar genes for NIT1 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with NIT1: view

No data available for Paralogs for NIT1 Gene

Variants for NIT1 Gene

Sequence variations from dbSNP and Humsavar for NIT1 Gene

SNP ID Clin Chr 01 pos Variation AA Info Type
rs1000050394 -- 161,119,877(+) G/A coding_sequence_variant, synonymous_variant
rs1000893680 -- 161,121,235(+) C/T 3_prime_UTR_variant, downstream_transcript_variant, genic_downstream_transcript_variant, intron_variant
rs1001560087 -- 161,124,615(+) G/A 3_prime_UTR_variant, genic_downstream_transcript_variant
rs1001596000 -- 161,124,893(+) C/T 3_prime_UTR_variant, genic_downstream_transcript_variant
rs1001725631 -- 161,124,025(+) A/G genic_downstream_transcript_variant, intron_variant

Variation tolerance for NIT1 Gene

Residual Variation Intolerance Score: 28.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.80; 79.09% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NIT1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NIT1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for NIT1 Gene

Disorders for NIT1 Gene

Additional Disease Information for NIT1

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for NIT1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NIT1 Gene

Publications for NIT1 Gene

  1. Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans. (PMID: 9671749) Pekarsky Y … Croce CM (Proceedings of the National Academy of Sciences of the United States of America 1998) 2 3 4 22 58
  2. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson SL … O'Brien SJ (PloS one 2010) 3 44 58
  3. Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor. (PMID: 16864578) Semba S … Huebner K (The Journal of biological chemistry 2006) 3 22 58
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58
  5. Architecture of the human interactome defines protein communities and disease networks. (PMID: 28514442) Huttlin EL … Harper JW (Nature 2017) 3 58

Products for NIT1 Gene

Sources for NIT1 Gene

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