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Aliases for NFS1 Gene

Aliases for NFS1 Gene

  • NFS1, Cysteine Desulfurase 2 3 5
  • Nitrogen Fixation 1 (S. Cerevisiae, Homolog) 2 3
  • NIFS 3 4
  • NFS1 Nitrogen Fixation 1 Homolog (S. Cerevisiae) 2
  • Nitrogen-Fixing Bacteria S-Like Protein 3
  • Cysteine Desulfurase, Mitochondrial 3
  • NFS1 Nitrogen Fixation 1 Homolog 3
  • EC 4
  • HUSSY-08 3
  • IscS 3

External Ids for NFS1 Gene

Previous GeneCards Identifiers for NFS1 Gene

  • GC20U990032
  • GC20M033975
  • GC20M034925
  • GC20M034972
  • GC20M033720
  • GC20M034256
  • GC20M034220
  • GC20M031036
  • GC20M034255

Summaries for NFS1 Gene

Entrez Gene Summary for NFS1 Gene

  • Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]

GeneCards Summary for NFS1 Gene

NFS1 (NFS1, Cysteine Desulfurase) is a Protein Coding gene. Diseases associated with NFS1 include Combined Oxidative Phosphorylation Deficiency 19 and Xanthinuria. Among its related pathways are Metabolism and Metabolism of water-soluble vitamins and cofactors. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and lyase activity. An important paralog of this gene is SCLY.

UniProtKB/Swiss-Prot for NFS1 Gene

  • Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.

Gene Wiki entry for NFS1 Gene

Additional gene information for NFS1 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NFS1 Gene

Genomics for NFS1 Gene

GeneHancer (GH) Regulatory Elements for NFS1 Gene

Promoters and enhancers for NFS1 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH20J035697 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 650.7 -0.2 -168 4.1 HDGF PKNOX1 FOXA2 MLX ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 NFS1 ROMO1 CPNE1 RBM12 RBM39 NCOA6 TRPC4AP ERGIC3 RPL36P4 AAR2
GH20J035764 Promoter/Enhancer 2.6 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 10.5 -70.2 -70180 9.2 MLX FEZF1 DMAP1 IRF4 YY1 SLC30A9 E2F8 ZNF416 ZNF548 SP3 PHF20 ENSG00000283527 CPNE1 RBM12 RBM39 NCOA6 ERGIC3 TRPC4AP AAR2 DLGAP4-AS1
GH20J035659 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 11.5 +36.5 36532 5.9 HDGF PKNOX1 SMAD1 MLX ZFP64 ARID4B SIN3A FEZF1 DMAP1 ZNF2 CPNE1 RBM12 RBM39 EIF6 ERGIC3 ROMO1 NFS1 TRPC4AP GGT7 PHF20
GH20J035874 Enhancer 1.6 FANTOM5 Ensembl ENCODE dbSUPER 12.3 -176.2 -176152 2.6 PKNOX1 ATF1 ARNT FEZF1 ZNF2 BRCA1 GTF3C2 ZNF213 KLF7 ATF7 CPNE1 RBM39 RBM12 DSN1 ERGIC3 RPL36P4 TRPC4AP NFS1 EIF6 AAR2
GH20J035881 Enhancer 1.7 FANTOM5 Ensembl ENCODE dbSUPER 10.9 -184.4 -184421 4.6 HDGF PKNOX1 ARID4B SIN3A FEZF1 ZNF2 ZNF48 GTF3C2 ZNF121 ELK1 CPNE1 RBM39 RBM12 ERGIC3 RPL36P4 TRPC4AP EIF6 DSN1 AAR2 RNU6-937P
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NFS1 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the NFS1 gene promoter:
  • ZIC2
  • C/EBPalpha
  • Msx-1
  • AML1a
  • Pbx1a
  • NF-kappaB1
  • GR
  • GR-alpha
  • GR-beta
  • NF-kappaB

Genomic Locations for NFS1 Gene

Genomic Locations for NFS1 Gene
31,311 bases
Minus strand
31,311 bases
Minus strand

Genomic View for NFS1 Gene

Genes around NFS1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NFS1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NFS1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NFS1 Gene

Proteins for NFS1 Gene

  • Protein details for NFS1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Cysteine desulfurase, mitochondrial
    Protein Accession:
    Secondary Accessions:
    • B3KMA5
    • B4DXK9
    • E1P5R8
    • F5GYK5
    • Q6P0L8
    • Q9NTZ5
    • Q9Y481

    Protein attributes for NFS1 Gene

    457 amino acids
    Molecular mass:
    50196 Da
    Name=pyridoxal 5-phosphate; Xref=ChEBI:CHEBI:597326;
    Quaternary structure:
    • Binds ISCU/NIFUN (PubMed:11060020). Forms a complex with LYRM4 (PubMed:18650437, PubMed:19454487). Interacts with HSPA9 (PubMed:26702583).

    Three dimensional structures from OCA and Proteopedia for NFS1 Gene

    Alternative splice isoforms for NFS1 Gene


neXtProt entry for NFS1 Gene

Post-translational modifications for NFS1 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for NFS1 Gene

Domains & Families for NFS1 Gene

Gene Families for NFS1 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
  • Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
genes like me logo Genes that share domains with NFS1: view

Function for NFS1 Gene

Molecular function for NFS1 Gene

UniProtKB/Swiss-Prot Function:
Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
UniProtKB/Swiss-Prot CatalyticActivity:
L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=434.75 uM for L-cysteine {ECO:0000269 PubMed:18650437}; Note=Kinetic parameter was determined for the protein lacking the 55 N-terminal amino acids and in a complex with LYRM4.;

Enzyme Numbers (IUBMB) for NFS1 Gene

Phenotypes From GWAS Catalog for NFS1 Gene

Gene Ontology (GO) - Molecular Function for NFS1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003824 catalytic activity IEA --
GO:0005515 protein binding IPI 16527810
GO:0016740 transferase activity IEA --
GO:0030170 pyridoxal phosphate binding IEA --
GO:0031071 cysteine desulfurase activity TAS,IEA --
genes like me logo Genes that share ontologies with NFS1: view
genes like me logo Genes that share phenotypes with NFS1: view

Animal Model Products

  • Taconic Biosciences Mouse Models for NFS1

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NFS1

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NFS1 Gene

Localization for NFS1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NFS1 Gene

Isoform Mitochondrial: Mitochondrion.
Isoform Cytoplasmic: Cytoplasm. Nucleus.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NFS1 gene
Compartment Confidence
mitochondrion 5
nucleus 5
cytosol 5

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Nucleoplasm (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NFS1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 16847322
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion TAS,IDA 26702583
GO:0005759 mitochondrial matrix IDA,TAS --
genes like me logo Genes that share ontologies with NFS1: view

Pathways & Interactions for NFS1 Gene

genes like me logo Genes that share pathways with NFS1: view

Gene Ontology (GO) - Biological Process for NFS1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000096 sulfur amino acid metabolic process TAS 9885568
GO:0006777 Mo-molybdopterin cofactor biosynthetic process IEA --
GO:0018283 iron incorporation into metallo-sulfur cluster IDA 16847322
GO:0032324 molybdopterin cofactor biosynthetic process TAS --
GO:0044281 small molecule metabolic process TAS --
genes like me logo Genes that share ontologies with NFS1: view

No data available for SIGNOR curated interactions for NFS1 Gene

Drugs & Compounds for NFS1 Gene

(11) Drugs for NFS1 Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
L-Alanine Approved Nutra Full agonist, Agonist, Target Glycine receptor agonist 0
L-Cysteine Approved Nutra Target 0
Pyridoxal Phosphate Approved, Investigational Nutra Target, cofactor 17

(5) Additional Compounds for NFS1 Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • FE (II) ion
  • Fe(II)
  • Fe(2+)
  • Ferrous ion
  • Iron ion(2+)
genes like me logo Genes that share compounds with NFS1: view

Transcripts for NFS1 Gene

mRNA/cDNA for NFS1 Gene

Unigene Clusters for NFS1 Gene

NFS1 nitrogen fixation 1 homolog (S. cerevisiae):
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NFS1

Alternative Splicing Database (ASD) splice patterns (SP) for NFS1 Gene

No ASD Table

Relevant External Links for NFS1 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NFS1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NFS1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NFS1 Gene

This gene is overexpressed in Adrenal (9.6) and Skin (6.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NFS1 Gene

Protein tissue co-expression partners for NFS1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NFS1 Gene:


SOURCE GeneReport for Unigene cluster for NFS1 Gene:


mRNA Expression by UniProt/SwissProt for NFS1 Gene:

Tissue specificity: Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas.

Evidence on tissue expression from TISSUES for NFS1 Gene

  • Nervous system(4.6)
  • Pancreas(4.2)

Phenotype-based relationships between genes and organs from Gene ORGANizer for NFS1 Gene

Germ Layers:
  • ectoderm
  • endoderm
  • mesoderm
  • digestive
  • nervous
  • respiratory
  • skeletal muscle
Head and neck:
  • brain
  • lung
  • liver
  • peripheral nerve
  • peripheral nervous system
  • spinal cord
genes like me logo Genes that share expression patterns with NFS1: view

No data available for mRNA differential expression in normal tissues for NFS1 Gene

Orthologs for NFS1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NFS1 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NFS1 34 33
  • 99.64 (n)
(Bos Taurus)
Mammalia NFS1 34 33
  • 92.85 (n)
(Canis familiaris)
Mammalia NFS1 34 33
  • 92.49 (n)
(Rattus norvegicus)
Mammalia Nfs1 33
  • 89.12 (n)
(Mus musculus)
Mammalia Nfs1 16 34 33
  • 88.03 (n)
(Monodelphis domestica)
Mammalia -- 34
  • 87 (a)
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 66 (a)
(Gallus gallus)
Aves NFS1 34 34
  • 82 (a)
LOC100858210 33
  • 73.94 (n)
(Anolis carolinensis)
Reptilia -- 34
  • 82 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nfs1 33
  • 73.63 (n)
Str.16278 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.6508 33
(Danio rerio)
Actinopterygii nfs1 34 33
  • 73.71 (n)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6217 33
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP009094 33
  • 67.75 (n)
fruit fly
(Drosophila melanogaster)
Insecta CG12264 34 35 33
  • 66.17 (n)
(Caenorhabditis elegans)
Secernentea B0205.6 34 35 33
  • 63.84 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AAL013W 33
  • 61.25 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NFS1 36 34 33
  • 60.42 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0D04928g 33
  • 59.25 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons NFS1 33
  • 62.18 (n)
(Glycine max)
eudicotyledons Gma.2991 33
(Oryza sativa)
Liliopsida Os09g0338400 33
  • 65.34 (n)
Os.9367 33
(Triticum aestivum)
Liliopsida Ta.10246 33
(Hordeum vulgare)
Liliopsida Hv.4410 33
(Zea mays)
Liliopsida Zm.4660 33
bread mold
(Neurospora crassa)
Ascomycetes NCU04636 33
  • 64.83 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes nfs1 33
  • 61.15 (n)
sea squirt
(Ciona savignyi)
Ascidiacea Cin.10386 33
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.10386 33
Species where no ortholog for NFS1 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for NFS1 Gene

Gene Tree for NFS1 (if available)
Gene Tree for NFS1 (if available)
Evolutionary constrained regions (ECRs) for NFS1: view image

Paralogs for NFS1 Gene

Paralogs for NFS1 Gene

(8) SIMAP similar genes for NFS1 Gene using alignment to 8 proteins:

  • A2A2M1_HUMAN
  • F2Z2E7_HUMAN
  • F8WB23_HUMAN
  • Q5QP19_HUMAN
  • Q8WV90_HUMAN Pseudogenes for NFS1 Gene

genes like me logo Genes that share paralogs with NFS1: view

Variants for NFS1 Gene

Sequence variations from dbSNP and Humsavar for NFS1 Gene

SNP ID Clin Chr 20 pos Variation AA Info Type
rs1057523264 likely-benign, not specified 35,697,786(-) A/T coding_sequence_variant, non_coding_transcript_variant, synonymous_variant
rs141257298 likely-benign, not specified 35,698,522(-) G/A/C coding_sequence_variant, missense_variant, non_coding_transcript_variant, synonymous_variant
rs147867038 likely-benign, not specified 35,673,620(-) A/G coding_sequence_variant, non_coding_transcript_variant, synonymous_variant
rs763462869 likely-benign, not specified 35,674,513(-) G/A coding_sequence_variant, non_coding_transcript_variant, synonymous_variant
rs772010519 likely-benign, not specified 35,675,171(-) G/A coding_sequence_variant, non_coding_transcript_variant, synonymous_variant

Structural Variations from Database of Genomic Variants (DGV) for NFS1 Gene

Variant ID Type Subtype PubMed ID
esv2671511 CNV deletion 23128226
esv3337733 CNV insertion 20981092
nsv1116861 CNV deletion 24896259
nsv1138952 CNV deletion 24896259
nsv473734 CNV novel sequence insertion 20440878
nsv526188 CNV gain 19592680

Variation tolerance for NFS1 Gene

Residual Variation Intolerance Score: 47.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.03; 21.18% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NFS1 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NFS1 Gene

Disorders for NFS1 Gene

MalaCards: The human disease database

(3) MalaCards diseases for NFS1 Gene - From: HGMD, Orphanet, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
combined oxidative phosphorylation deficiency 19
  • coxpd19
  • xanthinuria, type i
friedreich ataxia 1
  • frda
- elite association - COSMIC cancer census association via MalaCards
Search NFS1 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NFS1

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with NFS1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NFS1 Gene

Publications for NFS1 Gene

  1. Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to different subcellular compartments is regulated through alternative AUG utilization. (PMID: 9885568) Land T … Rouault TA (Molecular cell 1998) 2 3 4 22 58
  2. Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells. (PMID: 11060020) Tong WH … Rouault T (The EMBO journal 2000) 3 4 22 58
  3. Mitochondrial Hspa9/Mortalin regulates erythroid differentiation via iron-sulfur cluster assembly. (PMID: 26702583) Shan Y … Cortopassi G (Mitochondrion 2016) 3 4 58
  4. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson SL … O'Brien SJ (PloS one 2010) 3 44 58
  5. Human ISD11 is essential for both iron-sulfur cluster assembly and maintenance of normal cellular iron homeostasis. (PMID: 19454487) Shi Y … Rouault TA (Human molecular genetics 2009) 4 22 58

Products for NFS1 Gene

Sources for NFS1 Gene

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