This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes ... See more...

Aliases for NET1 Gene

Aliases for NET1 Gene

  • Neuroepithelial Cell Transforming 1 2 3 5
  • Neuroepithelial Cell-Transforming Gene 1 Protein 3 4
  • ARHGEF8 3 4
  • Guanine Nucleotide Regulatory Protein (Oncogene) 3
  • Rho Guanine Nucleotide Exchange Factor (GEF) 8 3
  • Epididymis Secretory Sperm Binding Protein 3
  • Rho Guanine Nucleotide Exchange Factor 8 4
  • Neuroepithelioma Transforming Gene 1 3
  • Small GTP-Binding Protein Regulator 3
  • P65 Net1 Proto-Oncogene Protein 3
  • Proto-Oncogene P65 Net1 4
  • NET1A 3

External Ids for NET1 Gene

Previous GeneCards Identifiers for NET1 Gene

  • GC10M005513
  • GC10P005428
  • GC10P005554
  • GC10P005408

Summaries for NET1 Gene

Entrez Gene Summary for NET1 Gene

  • This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

GeneCards Summary for NET1 Gene

NET1 (Neuroepithelial Cell Transforming 1) is a Protein Coding gene. Diseases associated with NET1 include Oppositional Defiant Disorder and Skin Squamous Cell Carcinoma. Among its related pathways are Signaling by GPCR and Signaling by Slit. Gene Ontology (GO) annotations related to this gene include guanyl-nucleotide exchange factor activity and GTP-Rho binding. An important paralog of this gene is ARHGEF3.

UniProtKB/Swiss-Prot Summary for NET1 Gene

  • Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Gene Wiki entry for NET1 Gene

Additional gene information for NET1 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NET1 Gene

Genomics for NET1 Gene

GeneHancer (GH) Regulatory Elements for NET1 Gene

Promoters and enhancers for NET1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NET1 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NET1

Top Transcription factor binding sites by QIAGEN in the NET1 gene promoter:
  • AML1a
  • GATA-1
  • GATA-2
  • GATA-3
  • Gfi-1
  • Pax-2
  • Pax-2a
  • STAT5B

Genomic Locations for NET1 Gene

Genomic Locations for NET1 Gene
chr10:5,412,551-5,459,056
(GRCh38/hg38)
Size:
46,506 bases
Orientation:
Plus strand
chr10:5,454,514-5,500,426
(GRCh37/hg19)
Size:
45,913 bases
Orientation:
Plus strand

Genomic View for NET1 Gene

Genes around NET1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NET1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NET1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NET1 Gene

Proteins for NET1 Gene

  • Protein details for NET1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q7Z628-ARHG8_HUMAN
    Recommended name:
    Neuroepithelial cell-transforming gene 1 protein
    Protein Accession:
    Q7Z628
    Secondary Accessions:
    • Q12773
    • Q96D82
    • Q99903
    • Q9UEN6

    Protein attributes for NET1 Gene

    Size:
    596 amino acids
    Molecular mass:
    67740 Da
    Quaternary structure:
    • Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts with the PDZ 1 domain of BAIAP1 (By similarity).
    SequenceCaution:
    • Sequence=AAB08847.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=AAB37683.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=CAA08974.1; Type=Frameshift; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for NET1 Gene

    Alternative splice isoforms for NET1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NET1 Gene

Post-translational modifications for NET1 Gene

  • Ubiquitination at Lys194, Lys301, and Lys489
  • Modification sites at PhosphoSitePlus

Other Protein References for NET1 Gene

No data available for DME Specific Peptides for NET1 Gene

Domains & Families for NET1 Gene

Gene Families for NET1 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for NET1 Gene

Blocks:
  • DH domain
  • Pleckstrin-like
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for NET1 Gene

GenScript: Design optimal peptide antigens:
  • Rho guanine nucleotide exchange factor 8 (ARHG8_HUMAN)
  • cDNA FLJ53956, highly similar to Neuroepithelial cell-transforming gene 1 protein (B4E1Y1_HUMAN)
  • cDNA FLJ50843, highly similar to Neuroepithelial cell-transforming gene 1 protein (Q5SQI5_HUMAN)
  • cDNA, FLJ96638, Homo sapiens neuroepithelial cell transforming gene 1 (NET1), mRNA (Q5SQI7_HUMAN)
genes like me logo Genes that share domains with NET1: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for NET1 Gene

Function for NET1 Gene

Molecular function for NET1 Gene

UniProtKB/Swiss-Prot Function:
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
UniProtKB/Swiss-Prot Induction:
By TGFB1. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR).

Phenotypes From GWAS Catalog for NET1 Gene

Gene Ontology (GO) - Molecular Function for NET1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005085 guanyl-nucleotide exchange factor activity TAS 8649828
GO:0005089 Rho guanyl-nucleotide exchange factor activity IEA --
GO:0005515 protein binding IPI 24550280
genes like me logo Genes that share ontologies with NET1: view
genes like me logo Genes that share phenotypes with NET1: view

Animal Models for NET1 Gene

MGI Knock Outs for NET1:
  • Net1 Net1<tm1Jafr>
  • Net1 Net1<tm1.2Jafr>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NET1

Clone Products

  • Addgene plasmids for NET1

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NET1 Gene

Localization for NET1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NET1 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NET1 gene
Compartment Confidence
nucleus 4
cytosol 4
plasma membrane 1
extracellular 1
cytoskeleton 1
endoplasmic reticulum 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NET1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with NET1: view

Pathways & Interactions for NET1 Gene

PathCards logo

SuperPathways for NET1 Gene

SuperPathway Contained pathways
1 p75 NTR receptor-mediated signalling
2 GPCR Pathway
.73
.62
.59
.58
3 G-AlphaQ Signaling
.47
.47
.40
4 Signaling by GPCR
5 fMLP Pathway
.39
.31
genes like me logo Genes that share pathways with NET1: view

Pathways by source for NET1 Gene

1 GeneGo (Thomson Reuters) pathway for NET1 Gene
  • Cytoskeleton remodeling_Role of PDGFs in cell migration
21 Qiagen pathways for NET1 Gene
  • Activation of PKC through GPCR
  • Breast Cancer Regulation by Stathmin1
  • CXCR4 Pathway
  • G-AlphaI Signaling
  • G-AlphaQ Signaling

Gene Ontology (GO) - Biological Process for NET1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001558 regulation of cell growth NAS 8649828
GO:0007165 signal transduction TAS 8649828
GO:0007186 G protein-coupled receptor signaling pathway TAS --
GO:0035023 regulation of Rho protein signal transduction IEA --
GO:0035556 intracellular signal transduction IEA --
genes like me logo Genes that share ontologies with NET1: view

No data available for SIGNOR curated interactions for NET1 Gene

Drugs & Compounds for NET1 Gene

(3) Drugs for NET1 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine diphosphate Experimental Pharma 0
Guanosine monophosphate Experimental Pharma 0
Guanosine triphosphate Experimental Pharma 0
genes like me logo Genes that share compounds with NET1: view

Transcripts for NET1 Gene

mRNA/cDNA for NET1 Gene

2 REFSEQ mRNAs :
21 NCBI additional mRNA sequence :
6 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NET1

Clone Products

  • Addgene plasmids for NET1

Alternative Splicing Database (ASD) splice patterns (SP) for NET1 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14
SP1: - - - - - -
SP2: - - - -
SP3: - - - -
SP4: -
SP5: -
SP6: - -
SP7: - - - - - - - - -
SP8: -
SP9:
SP10:

Relevant External Links for NET1 Gene

GeneLoc Exon Structure for
NET1

Expression for NET1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NET1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NET1 Gene

This gene is overexpressed in Serum (56.5) and Cerebrospinal fluid (12.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NET1 Gene



Protein tissue co-expression partners for NET1 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NET1

SOURCE GeneReport for Unigene cluster for NET1 Gene:

Hs.25155

mRNA Expression by UniProt/SwissProt for NET1 Gene:

Q7Z628-ARHG8_HUMAN
Tissue specificity: Widely expressed.

Evidence on tissue expression from TISSUES for NET1 Gene

  • Nervous system(4.3)
  • Eye(4.1)
genes like me logo Genes that share expression patterns with NET1: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NET1 Gene

Orthologs for NET1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NET1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NET1 31 30
  • 99.27 (n)
OneToOne
dog
(Canis familiaris)
Mammalia NET1 31 30
  • 85.38 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Net1 17 31 30
  • 85.32 (n)
rat
(Rattus norvegicus)
Mammalia Net1 30
  • 84.47 (n)
oppossum
(Monodelphis domestica)
Mammalia -- 31
  • 83 (a)
OneToMany
-- 31
  • 77 (a)
OneToMany
-- 31
  • 74 (a)
OneToMany
cow
(Bos Taurus)
Mammalia NET1 31 30
  • 82.17 (n)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NET1 31
  • 80 (a)
OneToOne
chicken
(Gallus gallus)
Aves NET1 31 30
  • 76.6 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NET1 31
  • 74 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia net1 30
  • 71.29 (n)
Str.5900 30
zebrafish
(Danio rerio)
Actinopterygii net1 31 30
  • 64.94 (n)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Gef64C 31
  • 4 (a)
ManyToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ROM1 33 31
  • 7 (a)
ROM2 31
  • 7 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 46 (a)
OneToMany
Species where no ortholog for NET1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NET1 Gene

ENSEMBL:
Gene Tree for NET1 (if available)
TreeFam:
Gene Tree for NET1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NET1: view image

Paralogs for NET1 Gene

(8) SIMAP similar genes for NET1 Gene using alignment to 5 proteins:

  • ARHG8_HUMAN
  • B4E1Y1_HUMAN
  • Q5SQI5_HUMAN
  • Q5SQI6_HUMAN
  • Q5SQI7_HUMAN

Pseudogenes.org Pseudogenes for NET1 Gene

genes like me logo Genes that share paralogs with NET1: view

Variants for NET1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NET1 Gene

SNP ID Clinical significance and condition Chr 10 pos Variation AA Info Type
709561 Benign: not provided 5,456,658(+) C/T NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT
717975 Benign: not provided 5,456,592(+) A/G NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT
735073 Benign: not provided 5,456,955(+) A/G NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT
769763 Benign: not provided 5,456,761(+) G/A MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT
769764 Benign: not provided 5,456,847(+) A/G NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for NET1 Gene

Structural Variations from Database of Genomic Variants (DGV) for NET1 Gene

Variant ID Type Subtype PubMed ID
esv2731718 CNV deletion 23290073
esv3622229 CNV loss 21293372
nsv1045141 CNV gain 25217958
nsv549886 CNV loss 21841781
nsv831774 CNV gain 17160897

Variation tolerance for NET1 Gene

Residual Variation Intolerance Score: 14.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.27; 52.88% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NET1 Gene

Human Gene Mutation Database (HGMD)
NET1
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NET1

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NET1 Gene

Disorders for NET1 Gene

MalaCards: The human disease database

(3) MalaCards diseases for NET1 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
oppositional defiant disorder
skin squamous cell carcinoma
  • epidermoid skin carcinoma
breast cancer
  • breast cancer, familial
- elite association - COSMIC cancer census association via MalaCards
Search NET1 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NET1

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with NET1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NET1 Gene

Publications for NET1 Gene

  1. Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. (PMID: 8649828) Chan AM … Miki T (Oncogene 1996) 2 3 4 23 54
  2. The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage. (PMID: 21373644) Srougi MC … Burridge K (PloS one 2011) 3 4 54
  3. Interaction of the RhoA exchange factor Net1 with discs large homolog 1 protects it from proteasome-mediated degradation and potentiates Net1 activity. (PMID: 19586902) Carr HS … Frost JA (The Journal of biological chemistry 2009) 3 23 54
  4. Coexpression of alpha6beta4 integrin and guanine nucleotide exchange factor Net1 identifies node-positive breast cancer patients at high risk for distant metastasis. (PMID: 19124484) Gilcrease MZ … Frost JA (Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2009) 3 23 54
  5. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. (PMID: 18827818) Murray D … Doran P (British journal of cancer 2008) 3 23 54

Products for NET1 Gene

  • Signalway ELISA kits for NET1
  • Signalway Proteins for NET1

Sources for NET1 Gene