This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009] See more...

Aliases for NENF Gene

Aliases for NENF Gene

  • Neudesin Neurotrophic Factor 2 3 5
  • Neudesin 2 3 4
  • Cell Immortalization-Related Protein 2 3 4
  • Secreted Protein Of Unknown Function 3 4
  • Neuron-Derived Neurotrophic Factor 3 4
  • CIR2 3 4
  • SPUF 3 4
  • Spinal Cord Injury Related Protein 10 3
  • Neuron Derived Neurotrophic Factor 2
  • Cell Growth-Inhibiting Protein 47 3
  • SCIRP10-Related Protein 3
  • Protein GIG47 4
  • SPUF Protein 4
  • SCIRP10 3

External Ids for NENF Gene

Previous GeneCards Identifiers for NENF Gene

  • GC01P208996
  • GC01P210672
  • GC01P212606
  • GC01P183282

Summaries for NENF Gene

Entrez Gene Summary for NENF Gene

  • This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009]

GeneCards Summary for NENF Gene

NENF (Neudesin Neurotrophic Factor) is a Protein Coding gene. Diseases associated with NENF include Combat Disorder and Deafness, Autosomal Dominant 9. Gene Ontology (GO) annotations related to this gene include growth factor activity and heme binding.

UniProtKB/Swiss-Prot Summary for NENF Gene

  • Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis (PubMed:22748190).

Additional gene information for NENF Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NENF Gene

Genomics for NENF Gene

GeneHancer (GH) Regulatory Elements for NENF Gene

Promoters and enhancers for NENF Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01J212430 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 500.7 -0.7 -714 4.2 NRF1 POLR2G PHF8 ZFX REST POLR2A ZBTB11 ZNF423 AFF1 ZBTB26 ENSG00000287445 LOC105372906 NENF LINC02608 PACC1 PPP2R5A DTL INTS7 ENSG00000212187 BATF3
GH01J212476 Enhancer 1.3 FANTOM5 ENCODE dbSUPER 11.9 +44.9 44886 1.7 CTCF SP1 GTF2E2 ZBTB11 REST RELA MYC RAD21 EP300 CTBP1 LINC02771 ATF3 NENF ENSG00000235862 FAM71A BATF3 PACC1 ENSG00000212187 VASH2 lnc-TMEM206-7
GH01J212469 Enhancer 1.2 FANTOM5 Ensembl ENCODE dbSUPER 12.1 +37.1 37114 2 JUND FOS POLR2A FOSL2 USF1 FOSL1 STAT3 FOXP2 DPF2 NRF1 LINC02771 NENF FAM71A ENSG00000235862 ATF3 PACC1 BATF3 ENSG00000212187 VASH2 MG828462
GH01J212497 Enhancer 0.8 ENCODE 11.2 +65.5 65494 1.1 FOXA1 MEF2C TBX3 FOXA2 CEBPG FOS SRF CEBPB MEF2B ZNF205 LINC02771 ATF3 NENF BATF3 PACC1 ENSG00000286213 lnc-TMEM206-8
GH01J212436 Enhancer 0.7 Ensembl dbSUPER 9.7 +4.5 4514 0.4 NFIC CEBPG CEBPB ZFHX2 IKZF1 ZNF384 ATF4 HLF EGR2 ATF3 NENF MG828462
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NENF on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NENF

Top Transcription factor binding sites by QIAGEN in the NENF gene promoter:
  • COMP1
  • Elk-1
  • IRF-7A
  • Lhx3a
  • LHX3b
  • Pax-4a
  • STAT1
  • STAT1alpha
  • STAT1beta
  • STAT5A

Genomic Locations for NENF Gene

Genomic Locations for NENF Gene
chr1:212,432,887-212,446,379
(GRCh38/hg38)
Size:
13,493 bases
Orientation:
Plus strand
chr1:212,606,229-212,619,721
(GRCh37/hg19)
Size:
13,493 bases
Orientation:
Plus strand

Genomic View for NENF Gene

Genes around NENF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NENF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NENF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NENF Gene

Proteins for NENF Gene

  • Protein details for NENF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UMX5-NENF_HUMAN
    Recommended name:
    Neudesin
    Protein Accession:
    Q9UMX5
    Secondary Accessions:
    • A1KYQ8
    • Q53FZ6
    • Q5TM90

    Protein attributes for NENF Gene

    Size:
    172 amino acids
    Molecular mass:
    18856 Da
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • Non-classical progesterone receptors involved in extranuclear signaling are classified in 2 groups: the class II progestin and adipoQ receptor (PAQR) family (also called mPRs) (PAQR5, PAQR6, PAQR7, PAQR8 and PAQR9) and the b5-like heme/steroid-binding protein family (also called MAPRs) (PGRMC1, PGRMC2, NENF and CYB5D2).

neXtProt entry for NENF Gene

Post-translational modifications for NENF Gene

  • Glycosylation at Thr48, Thr76, Thr92, Ser96, and Thr143
  • Ubiquitination at Lys79
  • Modification sites at PhosphoSitePlus

Other Protein References for NENF Gene

ENSEMBL proteins:
REFSEQ proteins:

Assay Products

  • Boster Bio ELISA Kits for NENF

No data available for DME Specific Peptides for NENF Gene

Domains & Families for NENF Gene

Gene Families for NENF Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted secreted proteins

Protein Domains for NENF Gene

Blocks:
  • Cytochrome b5
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for NENF Gene

GenScript: Design optimal peptide antigens:
  • Secreted protein of unknown function (NENF_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UMX5

UniProtKB/Swiss-Prot:

NENF_HUMAN :
  • The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
  • Belongs to the cytochrome b5 family. MAPR subfamily.
Domain:
  • The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
Family:
  • Belongs to the cytochrome b5 family. MAPR subfamily.
genes like me logo Genes that share domains with NENF: view

Function for NENF Gene

Molecular function for NENF Gene

UniProtKB/Swiss-Prot Function:
Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis (PubMed:22748190).
UniProtKB/Swiss-Prot Induction:
Up-regulated in immortal cells. Induced in estrogen receptor positive breast cancer expressing progesterone receptor.

Phenotypes From GWAS Catalog for NENF Gene

Gene Ontology (GO) - Molecular Function for NENF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008083 growth factor activity IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with NENF: view
genes like me logo Genes that share phenotypes with NENF: view

Animal Models for NENF Gene

MGI Knock Outs for NENF:
  • Nenf Nenf<tm1Mkga>

Animal Model Products

  • Taconic Biosciences Mouse Models for NENF

CRISPR Products

miRNA for NENF Gene

miRTarBase miRNAs that target NENF

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NENF

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NENF Gene

Localization for NENF Gene

Subcellular locations from UniProtKB/Swiss-Prot for NENF Gene

Secreted, extracellular space.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NENF gene
Compartment Confidence
nucleus 5
extracellular 4
mitochondrion 2
endoplasmic reticulum 2
plasma membrane 1
cytoskeleton 1
peroxisome 1
cytosol 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for NENF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005615 extracellular space IEA --
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm IDA --
genes like me logo Genes that share ontologies with NENF: view

Pathways & Interactions for NENF Gene

PathCards logo

SuperPathways for NENF Gene

No Data Available

Gene Ontology (GO) - Biological Process for NENF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007165 signal transduction IEA --
GO:0032099 negative regulation of appetite IEA,ISS --
GO:0043410 positive regulation of MAPK cascade IEA --
genes like me logo Genes that share ontologies with NENF: view

No data available for Pathways by source and SIGNOR curated interactions for NENF Gene

Drugs & Compounds for NENF Gene

(2) Drugs for NENF Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Iron Approved, Experimental Pharma 1496
heme Pharma Agonist 0
genes like me logo Genes that share compounds with NENF: view

Transcripts for NENF Gene

mRNA/cDNA for NENF Gene

1 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NENF

Alternative Splicing Database (ASD) splice patterns (SP) for NENF Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b · 4c
SP1: -
SP2:
SP3: - -
SP4: -

Relevant External Links for NENF Gene

GeneLoc Exon Structure for
NENF

Expression for NENF Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NENF Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NENF Gene

This gene is overexpressed in Peripheral blood mononuclear cells (12.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NENF Gene



Protein tissue co-expression partners for NENF Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NENF

SOURCE GeneReport for Unigene cluster for NENF Gene:

Hs.461787

mRNA Expression by UniProt/SwissProt for NENF Gene:

Q9UMX5-NENF_HUMAN
Tissue specificity: Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.

Evidence on tissue expression from TISSUES for NENF Gene

  • Nervous system(4.6)
  • Liver(4.2)
  • Lung(3.6)
  • Muscle(2.3)
  • Pancreas(2.2)
genes like me logo Genes that share expression patterns with NENF: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NENF Gene

Orthologs for NENF Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NENF Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NENF 31 30
  • 99.81 (n)
OneToOne
dog
(Canis familiaris)
Mammalia NENF 31
  • 91 (a)
OneToOne
cow
(Bos Taurus)
Mammalia NENF 31 30
  • 89.94 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Nenf 17 31 30
  • 89.8 (n)
rat
(Rattus norvegicus)
Mammalia Nenf 30
  • 88.66 (n)
oppossum
(Monodelphis domestica)
Mammalia NENF 31
  • 78 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 73 (a)
OneToMany
-- 31
  • 71 (a)
OneToMany
chicken
(Gallus gallus)
Aves NENF 31 30
  • 79.23 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NENF 31
  • 80 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nenf 30
  • 67.61 (n)
Str.6787 30
zebrafish
(Danio rerio)
Actinopterygii nenf 31 30
  • 68.97 (n)
OneToOne
wufa11a04 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.10963 30
fruit fly
(Drosophila melanogaster)
Insecta CG12056 31
  • 14 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea tag-131 31
  • 13 (a)
OneToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons MAPR2 30
  • 57.09 (n)
rice
(Oryza sativa)
Liliopsida Os02g0793700 30
  • 58.06 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9270 31
  • 15 (a)
OneToMany
Species where no ortholog for NENF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NENF Gene

ENSEMBL:
Gene Tree for NENF (if available)
TreeFam:
Gene Tree for NENF (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NENF: view image

Paralogs for NENF Gene

(2) SIMAP similar genes for NENF Gene using alignment to 1 proteins:

  • NENF_HUMAN

Pseudogenes.org Pseudogenes for NENF Gene

genes like me logo Genes that share paralogs with NENF: view

No data available for Paralogs for NENF Gene

Variants for NENF Gene

Additional dbSNP identifiers (rs#s) for NENF Gene

Structural Variations from Database of Genomic Variants (DGV) for NENF Gene

Variant ID Type Subtype PubMed ID
nsv1119756 CNV deletion 24896259
nsv527863 CNV loss 19592680
nsv549139 CNV gain 21841781

Variation tolerance for NENF Gene

Residual Variation Intolerance Score: 54.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.86; 18.05% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NENF Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NENF

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NENF Gene

Disorders for NENF Gene

MalaCards: The human disease database

(3) MalaCards diseases for NENF Gene - From: DISEASES

Disorder Aliases PubMed IDs
combat disorder
  • combat neurosis
deafness, autosomal dominant 9
  • dfna9
acute stress disorder
  • traumatic stress disorder
- elite association - COSMIC cancer census association via MalaCards
Search NENF in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NENF

genes like me logo Genes that share disorders with NENF: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NENF Gene

Publications for NENF Gene

  1. Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity. (PMID: 15605373) Kimura I … Itoh N (Journal of neuroscience research 2005) 2 3 4 23 54
  2. Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines. (PMID: 9771976) Ma L … Bacchetti S (Oncogene 1998) 2 3 4 54
  3. Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain. (PMID: 18056703) Kimura I … Fujimoto M (The Journal of biological chemistry 2008) 3 23 54
  4. Breast cancer proteomics by laser capture microdissection, sample pooling, 54-cm IPG IEF, and differential iodine radioisotope detection. (PMID: 16645950) Neubauer H … Cahill MA (Electrophoresis 2006) 4 23 54
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54

Products for NENF Gene

Sources for NENF Gene