This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016] See more...

Aliases for NEMF Gene

Aliases for NEMF Gene

  • Nuclear Export Mediator Factor 2 3 5
  • Serologically Defined Colon Cancer Antigen 1 2 3 4
  • Nuclear Export Mediator Factor NEMF 3 4
  • Antigen NY-CO-1 3 4
  • SDCCAG1 3 4
  • NY-CO-1 3

External Ids for NEMF Gene

Previous HGNC Symbols for NEMF Gene

  • SDCCAG1

Previous GeneCards Identifiers for NEMF Gene

  • GC14M050250

Summaries for NEMF Gene

Entrez Gene Summary for NEMF Gene

  • This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016]

GeneCards Summary for NEMF Gene

NEMF (Nuclear Export Mediator Factor) is a Protein Coding gene. Diseases associated with NEMF include Atrial Septal Defect 6.

UniProtKB/Swiss-Prot Summary for NEMF Gene

  • Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875).

Additional gene information for NEMF Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NEMF Gene

Genomics for NEMF Gene

GeneHancer (GH) Regulatory Elements for NEMF Gene

Promoters and enhancers for NEMF Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NEMF on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NEMF

Genomic Locations for NEMF Gene

Genomic Locations for NEMF Gene
chr14:49,782,083-49,853,073
(GRCh38/hg38)
Size:
70,991 bases
Orientation:
Minus strand
chr14:50,249,997-50,319,921
(GRCh37/hg19)
Size:
69,925 bases
Orientation:
Minus strand

Genomic View for NEMF Gene

Genes around NEMF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEMF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEMF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEMF Gene

Proteins for NEMF Gene

  • Protein details for NEMF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O60524-NEMF_HUMAN
    Recommended name:
    Nuclear export mediator factor NEMF
    Protein Accession:
    O60524
    Secondary Accessions:
    • A0JLQ3
    • B3KSK1
    • B4DDL3
    • B4DHA9
    • B4E3F3
    • Q32Q66
    • Q8WW70
    • Q9NWG1

    Protein attributes for NEMF Gene

    Size:
    1076 amino acids
    Molecular mass:
    122954 Da
    Quaternary structure:
    • Component of the ribosome quality control complex (RQC), composed of at least the E3 ubiquitin ligase LTN1 and NEMF (PubMed:25578875). The complex probably also contains TCF25 as well as VCP/p97 and its ubiquitin-binding cofactors (By similarity). RQC forms a stable complex with 60S ribosomal subunits (PubMed:25578875). Interacts (via its N-terminus) with XPO1 (PubMed:16103875).
    Miscellaneous:
    • Active in normal lung tissue, but is inactive in several lung cancer cell lines.
    SequenceCaution:
    • Sequence=AAC18036.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=AAH06001.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=AAH20794.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=AAH56687.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=AAI07765.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=BAG52763.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=BAG58070.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305}; Sequence=BAG58070.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=BAG58070.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for NEMF Gene

    Alternative splice isoforms for NEMF Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEMF Gene

Post-translational modifications for NEMF Gene

  • Ubiquitination at Lys35
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for NEMF Gene

Domains & Families for NEMF Gene

Gene Families for NEMF Gene

Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for NEMF Gene

Blocks:
  • Protein of unknown function DUF814
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for NEMF Gene

GenScript: Design optimal peptide antigens:
  • Serologically defined colon cancer antigen 1 (NEMF_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O60524

UniProtKB/Swiss-Prot:

NEMF_HUMAN :
  • The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins (By similarity).
  • Belongs to the NEMF family.
Domain:
  • The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins (By similarity).
Family:
  • Belongs to the NEMF family.
genes like me logo Genes that share domains with NEMF: view

Function for NEMF Gene

Molecular function for NEMF Gene

UniProtKB/Swiss-Prot Function:
Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875).

Phenotypes From GWAS Catalog for NEMF Gene

Gene Ontology (GO) - Molecular Function for NEMF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000049 tRNA binding IBA 21873635
GO:0043023 ribosomal large subunit binding IBA 21873635
genes like me logo Genes that share ontologies with NEMF: view
genes like me logo Genes that share phenotypes with NEMF: view

Animal Models for NEMF Gene

MGI Knock Outs for NEMF:
  • Nemf Nemf<tm1b(EUCOMM)Hmgu>

Animal Model Products

CRISPR Products

miRNA for NEMF Gene

miRTarBase miRNAs that target NEMF

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NEMF

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NEMF Gene

Localization for NEMF Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEMF Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NEMF gene
Compartment Confidence
nucleus 4
cytosol 3
cytoskeleton 1
mitochondrion 1
endoplasmic reticulum 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nuclear bodies (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NEMF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:1990112 RQC complex IBA 21873635
genes like me logo Genes that share ontologies with NEMF: view

Pathways & Interactions for NEMF Gene

PathCards logo

SuperPathways for NEMF Gene

No Data Available

Gene Ontology (GO) - Biological Process for NEMF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0051168 nuclear export IMP 16103875
GO:0072344 rescue of stalled ribosome IBA 21873635
GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process IBA 21873635
genes like me logo Genes that share ontologies with NEMF: view

No data available for Pathways by source and SIGNOR curated interactions for NEMF Gene

Drugs & Compounds for NEMF Gene

No Compound Related Data Available

Transcripts for NEMF Gene

mRNA/cDNA for NEMF Gene

2 REFSEQ mRNAs :
26 NCBI additional mRNA sequence :
15 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NEMF

Alternative Splicing Database (ASD) splice patterns (SP) for NEMF Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^ 21 ^
SP1: -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8: -
SP9:

ExUns: 22 ^ 23 ^ 24a · 24b ^ 25 ^ 26 ^ 27a · 27b
SP1:
SP2:
SP3:
SP4:
SP5: -
SP6:
SP7:
SP8:
SP9:

Relevant External Links for NEMF Gene

GeneLoc Exon Structure for
NEMF

Expression for NEMF Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NEMF Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NEMF Gene

This gene is overexpressed in Lung (32.9) and Breast (14.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NEMF Gene



Protein tissue co-expression partners for NEMF Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NEMF

SOURCE GeneReport for Unigene cluster for NEMF Gene:

Hs.655964

mRNA Expression by UniProt/SwissProt for NEMF Gene:

O60524-NEMF_HUMAN
Tissue specificity: Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.

Evidence on tissue expression from TISSUES for NEMF Gene

  • Nervous system(4.7)
  • Intestine(4.3)
  • Kidney(4.3)
  • Liver(4.3)
genes like me logo Genes that share expression patterns with NEMF: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NEMF Gene

Orthologs for NEMF Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NEMF Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NEMF 31 30
  • 99.54 (n)
OneToOne
dog
(Canis familiaris)
Mammalia NEMF 31 30
  • 93.43 (n)
OneToOne
cow
(Bos Taurus)
Mammalia NEMF 31 30
  • 92.4 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Nemf 17 31 30
  • 88.57 (n)
rat
(Rattus norvegicus)
Mammalia Nemf 30
  • 87.82 (n)
oppossum
(Monodelphis domestica)
Mammalia NEMF 31
  • 81 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NEMF 31
  • 78 (a)
OneToOne
chicken
(Gallus gallus)
Aves NEMF 31 30
  • 73.92 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 31
  • 85 (a)
OneToMany
-- 31
  • 84 (a)
OneToMany
-- 31
  • 57 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia nemf 30
  • 70.33 (n)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.12972 30
zebrafish
(Danio rerio)
Actinopterygii Dr.10362 30
fruit fly
(Drosophila melanogaster)
Insecta Clbn 31 30
  • 57.82 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002680 30
  • 51.58 (n)
worm
(Caenorhabditis elegans)
Secernentea Y82E9BR.18 31 30
  • 52.92 (n)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TAE2 31
  • 28 (a)
OneToOne
RQC2 33
thale cress
(Arabidopsis thaliana)
eudicotyledons emb1441 30
  • 51.37 (n)
rice
(Oryza sativa)
Liliopsida Os12g0564600 30
  • 50.05 (n)
Os.26814 30
wheat
(Triticum aestivum)
Liliopsida Ta.8214 30
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 50 (a)
OneToOne
Cin.8376 30
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPCC132.01c 30
  • 48.64 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.8376 30
Species where no ortholog for NEMF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for NEMF Gene

ENSEMBL:
Gene Tree for NEMF (if available)
TreeFam:
Gene Tree for NEMF (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NEMF: view image

Paralogs for NEMF Gene

No data available for Paralogs for NEMF Gene

Variants for NEMF Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NEMF Gene

SNP ID Clinical significance and condition Chr 14 pos Variation AA Info Type
rs864622015 Uncertain Significance: Malignant tumor of prostate 49,799,507(-) CTG/C FRAMESHIFT_VARIANT
rs3100906 - p.Ser257Cys

Additional dbSNP identifiers (rs#s) for NEMF Gene

Structural Variations from Database of Genomic Variants (DGV) for NEMF Gene

Variant ID Type Subtype PubMed ID
esv28926 CNV gain+loss 19812545
nsv1138299 CNV deletion 24896259
nsv509533 CNV insertion 20534489

Variation tolerance for NEMF Gene

Residual Variation Intolerance Score: 7.66% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.93; 35.93% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NEMF Gene

Human Gene Mutation Database (HGMD)
NEMF
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NEMF

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NEMF Gene

Disorders for NEMF Gene

MalaCards: The human disease database

(1) MalaCards diseases for NEMF Gene - From: DISEASES

Disorder Aliases PubMed IDs
atrial septal defect 6
  • asd6
- elite association - COSMIC cancer census association via MalaCards
Search NEMF in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NEMF

genes like me logo Genes that share disorders with NEMF: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NEMF Gene

Publications for NEMF Gene

  1. Characterization of human colon cancer antigens recognized by autologous antibodies. (PMID: 9610721) Scanlan MJ … Old LJ (International journal of cancer 1998) 2 3 4 54
  2. Structure and assembly pathway of the ribosome quality control complex. (PMID: 25578875) Shao S … Hegde RS (Molecular cell 2015) 3 4 54
  3. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen JV … Mann M (Cell 2006) 3 4 54
  4. Drosophila caliban, a nuclear export mediator, can function as a tumor suppressor in human lung cancer cells. (PMID: 16103875) Bi X … Mortin MA (Oncogene 2005) 3 4 54
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54

Products for NEMF Gene

  • Boster Bio Antibodies for NEMF
  • Boster Bio ELISA Kits for NEMF

Sources for NEMF Gene