NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002] See more...

Aliases for NEK7 Gene

Aliases for NEK7 Gene

  • NIMA Related Kinase 7 2 3 5
  • NIMA (Never In Mitosis Gene A)-Related Kinase 7 2 3
  • Serine/Threonine-Protein Kinase Nek7 3 4
  • Never In Mitosis A-Related Kinase 7 3 4
  • NimA-Related Protein Kinase 7 3 4
  • EC 2.7.11.1 4 51
  • NEK7 5

External Ids for NEK7 Gene

Previous GeneCards Identifiers for NEK7 Gene

  • GC01P195928
  • GC01P193660
  • GC01P194592
  • GC01P195489
  • GC01P195459
  • GC01P194933
  • GC01P196392
  • GC01P198126
  • GC01P169281

Summaries for NEK7 Gene

Entrez Gene Summary for NEK7 Gene

  • NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]

GeneCards Summary for NEK7 Gene

NEK7 (NIMA Related Kinase 7) is a Protein Coding gene. Among its related pathways are Mitotic Prophase and Transport of the SLBP independent Mature mRNA. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is NEK6.

UniProtKB/Swiss-Prot Summary for NEK7 Gene

  • Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NEK7 Gene

Genomics for NEK7 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for NEK7 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01J198154 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 603.4 +0.9 922 6.2 CEBPG SP1 GATAD2A CTCF PRDM10 IKZF1 ZNF692 POLR2A BACH1 FOS NEK7 NONHSAG003872.2 lnc-ATP6V1G3-10 ZBTB41 DENND1B PTPRC C1orf53 lnc-NEK7-1 lnc-ATP6V1G3-9 ATP6V1G3
GH01J198211 Promoter 0.3 EPDnew 600.2 +54.7 54700 0.1 NEK7 PRR13P1 NONHSAG003872.2 HSALNG0009493 ATP6V1G3
GH01J198185 Enhancer 0.9 ENCODE dbSUPER 12.5 +30.6 30579 4.7 JUND PKNOX1 MTA1 DPF2 HDAC1 SOX6 HMBOX1 CEBPB CTBP1 NFE2 NEK7 lnc-ATP6V1G3-9 NONHSAG003872.2 ATP6V1G3
GH01J198177 Enhancer 0.8 ENCODE dbSUPER 11.9 +22.4 22357 4.3 ZSCAN4 IKZF2 ZBTB20 ZXDB CEBPB RUNX3 ZNF316 ZSCAN5C GABPA MAFG NEK7 lnc-ATP6V1G3-9 NONHSAG003872.2 ATP6V1G3
GH01J198294 Enhancer 1.1 VISTA UCNEbase Ensembl 4.1 +138.4 138413 2.4 POLR2A ATF4 SMARCA4 HLF NEK7 HSALNG0009493 PRR13P1 ATP6V1G3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NEK7 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NEK7

Top Transcription factor binding sites by QIAGEN in the NEK7 gene promoter:
  • GATA-1
  • ISGF-3
  • Oct-B1
  • oct-B2
  • oct-B3
  • POU2F1
  • POU2F1a
  • POU2F2
  • POU2F2 (Oct-2.1)
  • POU2F2B

Genomic Locations for NEK7 Gene

Genomic Locations for NEK7 Gene
chr1:198,156,994-198,322,420
(GRCh38/hg38)
Size:
165,427 bases
Orientation:
Plus strand
chr1:198,126,093-198,291,550
(GRCh37/hg19)
Size:
165,458 bases
Orientation:
Plus strand

Genomic View for NEK7 Gene

Genes around NEK7 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEK7 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEK7 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEK7 Gene

Proteins for NEK7 Gene

  • Protein details for NEK7 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8TDX7-NEK7_HUMAN
    Recommended name:
    Serine/threonine-protein kinase Nek7
    Protein Accession:
    Q8TDX7
    Secondary Accessions:
    • A6NGT8

    Protein attributes for NEK7 Gene

    Size:
    302 amino acids
    Molecular mass:
    34551 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Monomer (PubMed:21320329). Interacts with NEK9 (PubMed:19414596). Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation (PubMed:26188091).

    Three dimensional structures from OCA and Proteopedia for NEK7 Gene

    Alternative splice isoforms for NEK7 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEK7 Gene

Selected DME Specific Peptides for NEK7 Gene

Q8TDX7:
  • IEKKIGRGQFSEVY
  • GTPYYMSPE
  • FKKQKRLIPERTVWKYFVQLCSA
  • KSDIWSLGC
  • KIEQCDYPPLP
  • KPANVFIT
  • SFIEDNELNIVLELADAGDLS

Post-translational modifications for NEK7 Gene

  • Phosphorylation at Ser-195 required for its activation.
  • Ubiquitination at Lys74 and Lys189
  • Modification sites at PhosphoSitePlus

Domains & Families for NEK7 Gene

Gene Families for NEK7 Gene

Suggested Antigen Peptide Sequences for NEK7 Gene

GenScript: Design optimal peptide antigens:
  • Never in mitosis A-related kinase 7 (NEK7_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8TDX7

UniProtKB/Swiss-Prot:

NEK7_HUMAN :
  • Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Domain:
  • Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
  • The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.
Family:
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
genes like me logo Genes that share domains with NEK7: view

Function for NEK7 Gene

Molecular function for NEK7 Gene

UniProtKB/Swiss-Prot Function:
Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97.

Enzyme Numbers (IUBMB) for NEK7 Gene

Phenotypes From GWAS Catalog for NEK7 Gene

Gene Ontology (GO) - Molecular Function for NEK7 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity ISS --
GO:0005515 protein binding IPI 26188091
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with NEK7: view
genes like me logo Genes that share phenotypes with NEK7: view

Animal Models for NEK7 Gene

MGI Knock Outs for NEK7:
  • Nek7 Nek7<tm1Mot>
  • Nek7 Nek7<tm1b(EUCOMM)Hmgu>

Animal Model Products

  • Taconic Biosciences Mouse Models for NEK7

CRISPR Products

miRNA for NEK7 Gene

miRTarBase miRNAs that target NEK7

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NEK7

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NEK7 Gene

Localization for NEK7 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEK7 Gene

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity). {ECO:0000250 UniProtKB:Q9ES74, ECO:0000269 PubMed:17586473}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NEK7 gene
Compartment Confidence
nucleus 5
cytoskeleton 4
cytosol 4
extracellular 2
mitochondrion 2
lysosome 2
plasma membrane 1
endoplasmic reticulum 1
endosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for NEK7 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000922 spindle pole IEA --
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm ISS --
GO:0005815 microtubule organizing center IEA --
genes like me logo Genes that share ontologies with NEK7: view

Pathways & Interactions for NEK7 Gene

genes like me logo Genes that share pathways with NEK7: view

Pathways by source for NEK7 Gene

1 Cell Signaling Technology pathway for NEK7 Gene

SIGNOR curated interactions for NEK7 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for NEK7 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA,IDA 12840024
GO:0007346 regulation of mitotic cell cycle TAS 17727698
GO:0016310 phosphorylation IEA --
GO:0032212 positive regulation of telomere maintenance via telomerase IMP 21531765
GO:0051225 spindle assembly TAS 17727698
genes like me logo Genes that share ontologies with NEK7: view

Drugs & Compounds for NEK7 Gene

(2) Drugs for NEK7 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Approved, Experimental, Investigational Pharma 0
ATP Investigational Nutra Agonist, Activator, Partial agonist, Antagonist, Full agonist, Gating inhibitor, Pore Blocker, Potentiation 0

(1) Additional Compounds for NEK7 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Agonist, Full agonist, Partial agonist, Gating inhibitor, Antagonist 58-64-0
genes like me logo Genes that share compounds with NEK7: view

Transcripts for NEK7 Gene

mRNA/cDNA for NEK7 Gene

1 REFSEQ mRNAs :
5 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NEK7

Alternative Splicing Database (ASD) splice patterns (SP) for NEK7 Gene

No ASD Table

Relevant External Links for NEK7 Gene

GeneLoc Exon Structure for
NEK7

Expression for NEK7 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NEK7 Gene

mRNA differential expression in normal tissues according to GTEx for NEK7 Gene

This gene is overexpressed in Heart - Left Ventricle (x4.4).

Protein differential expression in normal tissues from HIPED for NEK7 Gene

This gene is overexpressed in Breast (13.2) and Peripheral blood mononuclear cells (7.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NEK7 Gene



Protein tissue co-expression partners for NEK7 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NEK7

SOURCE GeneReport for Unigene cluster for NEK7 Gene:

Hs.24119

mRNA Expression by UniProt/SwissProt for NEK7 Gene:

Q8TDX7-NEK7_HUMAN
Tissue specificity: Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

Evidence on tissue expression from TISSUES for NEK7 Gene

  • Pancreas(4.3)
  • Nervous system(3.7)
  • Blood(2.7)
  • Heart(2.6)
  • Spleen(2.5)
  • Muscle(2.4)
  • Kidney(2.3)
  • Lung(2.3)
  • Lymph node(2.2)
  • Intestine(2.2)
  • Liver(2)
genes like me logo Genes that share expression patterns with NEK7: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and Phenotype-based relationships between genes and organs from Gene ORGANizer for NEK7 Gene

Orthologs for NEK7 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NEK7 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia NEK7 30 31
  • 99.89 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia NEK7 31
  • 99 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia NEK7 30 31
  • 94.92 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia NEK7 30 31
  • 91.39 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Nek7 30 17 31
  • 91.28 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Nek7 30
  • 91.06 (n)
Platypus
(Ornithorhynchus anatinus)
Mammalia NEK7 31
  • 90 (a)
OneToOne
Chicken
(Gallus gallus)
Aves NEK7 30 31
  • 85.98 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia NEK7 31
  • 96 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia nek7 30
  • 80.91 (n)
Zebrafish
(Danio rerio)
Actinopterygii nek7 30 31
  • 76.43 (n)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea nekl-3 30 31
  • 65.79 (n)
OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes KIN3 31
  • 21 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 68 (a)
OneToMany
Species where no ortholog for NEK7 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for NEK7 Gene

ENSEMBL:
Gene Tree for NEK7 (if available)
TreeFam:
Gene Tree for NEK7 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NEK7: view image

Paralogs for NEK7 Gene

Paralogs for NEK7 Gene

(47) SIMAP similar genes for NEK7 Gene using alignment to 4 proteins:

  • NEK7_HUMAN
  • C9J1H8_HUMAN
  • F5H3U7_HUMAN
  • F8WAG2_HUMAN
genes like me logo Genes that share paralogs with NEK7: view

Variants for NEK7 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NEK7 Gene

SNP ID Clinical significance and condition Chr 01 pos Variation AA Info Type
VAR_040925 An ovarian serous carcinoma sample p.Ile275Met
rs55833332 - p.Arg35Gly

Additional dbSNP identifiers (rs#s) for NEK7 Gene

Structural Variations from Database of Genomic Variants (DGV) for NEK7 Gene

Variant ID Type Subtype PubMed ID
esv2564043 CNV deletion 19546169
esv3304305 CNV mobile element insertion 20981092
esv3305151 CNV mobile element insertion 20981092
esv3309071 CNV mobile element insertion 20981092
esv3328419 CNV insertion 20981092
esv3588513 CNV loss 21293372
esv3588517 CNV loss 21293372
esv3588519 CNV loss 21293372
esv3588521 CNV loss 21293372
nsv1005959 CNV gain 25217958
nsv1011794 CNV gain 25217958
nsv1141162 CNV duplication 24896259
nsv1145391 CNV deletion 24896259
nsv832248 CNV gain+loss 17160897
nsv946568 CNV duplication 23825009
nsv952635 CNV duplication 24416366

Variation tolerance for NEK7 Gene

Residual Variation Intolerance Score: 29.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.49; 29.09% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NEK7 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NEK7

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NEK7 Gene

Disorders for NEK7 Gene

Additional Disease Information for NEK7

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform

No disorders were found for NEK7 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NEK7 Gene

Publications for NEK7 Gene

  1. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. (PMID: 11701951) Kimura M … Okano Y (Cytogenetics and cell genetics 2001) 2 3 4 23
  2. Anks3 alters the sub-cellular localization of the Nek7 kinase. (PMID: 26188091) Ramachandran H … Yakulov TA (Biochemical and biophysical research communications 2015) 3 4
  3. Centrosome-related genes, genetic variation, and risk of breast cancer. (PMID: 20508983) Olson JE … Couch FJ (Breast cancer research and treatment 2011) 3 41
  4. An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9. (PMID: 19941817) Richards MW … Bayliss R (Molecular cell 2009) 3 4
  5. The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. (PMID: 19414596) O'Regan L … Fry AM (Molecular and cellular biology 2009) 3 4

Products for NEK7 Gene

Sources for NEK7 Gene