This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and ac... See more...

Aliases for NEK3 Gene

Aliases for NEK3 Gene

  • NIMA Related Kinase 3 2 3 5
  • NIMA (Never In Mitosis Gene A)-Related Kinase 3 2 3
  • Serine/Threonine-Protein Kinase Nek3 3 4
  • Never In Mitosis A-Related Kinase 3 3 4
  • NimA-Related Protein Kinase 3 3 4
  • Phosphorylase B Kinase Kinase 2 3
  • Hydroxyalkyl-Protein Kinase 2 3
  • Glycogen Synthase A Kinase 2 3
  • EC 2.7.11.1 4 52
  • HSPK 36 3 4
  • Serine/Threonine-Protein Kinase NEK3 2
  • EC 2.7.11 52
  • HSPK36 3

External Ids for NEK3 Gene

Previous GeneCards Identifiers for NEK3 Gene

  • GC13U990024
  • GC13M046694
  • GC13M051643
  • GC13M050504
  • GC13M051604
  • GC13M052706
  • GC13M033494

Summaries for NEK3 Gene

Entrez Gene Summary for NEK3 Gene

  • This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon. [provided by RefSeq, Sep 2019]

GeneCards Summary for NEK3 Gene

NEK3 (NIMA Related Kinase 3) is a Protein Coding gene. Diseases associated with NEK3 include Muscular Phosphorylase Kinase Deficiency and Glycogen Storage Disease Ia. Among its related pathways are Prolactin Signaling Pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is NEK5.

UniProtKB/Swiss-Prot Summary for NEK3 Gene

  • Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.

Gene Wiki entry for NEK3 Gene

Additional gene information for NEK3 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NEK3 Gene

Genomics for NEK3 Gene

GeneHancer (GH) Regulatory Elements for NEK3 Gene

Promoters and enhancers for NEK3 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NEK3 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NEK3

Top Transcription factor binding sites by QIAGEN in the NEK3 gene promoter:
  • AML1a
  • E47
  • HOXA3
  • PPAR-gamma1
  • PPAR-gamma2

Genomic Locations for NEK3 Gene

Genomic Locations for NEK3 Gene
chr13:52,132,639-52,159,861
(GRCh38/hg38)
Size:
27,223 bases
Orientation:
Minus strand
chr13:52,706,775-52,733,996
(GRCh37/hg19)
Size:
27,222 bases
Orientation:
Minus strand

Genomic View for NEK3 Gene

Genes around NEK3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEK3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEK3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEK3 Gene

Proteins for NEK3 Gene

  • Protein details for NEK3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P51956-NEK3_HUMAN
    Recommended name:
    Serine/threonine-protein kinase Nek3
    Protein Accession:
    P51956
    Secondary Accessions:
    • A8K2J4
    • Q5TAP2
    • Q8J023
    • Q8WUN5

    Protein attributes for NEK3 Gene

    Size:
    506 amino acids
    Molecular mass:
    57705 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent.
    SequenceCaution:
    • Sequence=EAX08907.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

    Alternative splice isoforms for NEK3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEK3 Gene

Selected DME Specific Peptides for NEK3 Gene

P51956:
  • MIGEGSFGR
  • HRDIKSKN
  • KSDIWSLGC

Post-translational modifications for NEK3 Gene

  • Phosphorylation at Thr-479 regulates its catalytic activity.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

Domains & Families for NEK3 Gene

Gene Families for NEK3 Gene

Protein Domains for NEK3 Gene

Suggested Antigen Peptide Sequences for NEK3 Gene

GenScript: Design optimal peptide antigens:
  • Never in mitosis A-related kinase 3 (NEK3_HUMAN)
  • NIMA (Never in mitosis gene a)-related kinase 3 (Q5TAP3_HUMAN)
  • cDNA FLJ16392 fis, clone TRACH2020048, moderately similar to Mus musculus serine/threonine-protein kinase NEK3 (Nek3) mRNA (Q6ZN64_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P51956

UniProtKB/Swiss-Prot:

NEK3_HUMAN :
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Family:
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
genes like me logo Genes that share domains with NEK3: view

Function for NEK3 Gene

Molecular function for NEK3 Gene

UniProtKB/Swiss-Prot Function:
Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Prolactin stimulates its activity.
GENATLAS Biochemistry:
never in mitosis gene A-related kinase 3,predominantly cytoplasmic,widely expressed in a non dependent cell cycle pattern

Enzyme Numbers (IUBMB) for NEK3 Gene

Phenotypes From GWAS Catalog for NEK3 Gene

Gene Ontology (GO) - Molecular Function for NEK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity NAS 7522034
GO:0005515 protein binding IPI 15618286
GO:0005524 ATP binding IEA --
GO:0016301 kinase activity IEA --
genes like me logo Genes that share ontologies with NEK3: view
genes like me logo Genes that share phenotypes with NEK3: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NEK3

Clone Products

  • Addgene plasmids for NEK3

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NEK3 Gene

Localization for NEK3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEK3 Gene

Cytoplasm. Cell projection, axon.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NEK3 gene
Compartment Confidence
nucleus 5
cytosol 2
plasma membrane 1
extracellular 1
cytoskeleton 1
golgi apparatus 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for NEK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus NAS 7522034
GO:0005737 cytoplasm IEA --
GO:0030424 axon IEA --
GO:0042995 cell projection IEA --
genes like me logo Genes that share ontologies with NEK3: view

Pathways & Interactions for NEK3 Gene

PathCards logo

SuperPathways for NEK3 Gene

SuperPathway Contained pathways
1 Prolactin Signaling Pathway
.38
genes like me logo Genes that share pathways with NEK3: view

Pathways by source for NEK3 Gene

1 BioSystems pathway for NEK3 Gene
1 GeneGo (Thomson Reuters) pathway for NEK3 Gene
  • Development Prolactin receptor signaling

Gene Ontology (GO) - Biological Process for NEK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000278 mitotic cell cycle NAS 7522034
GO:0006468 protein phosphorylation ISS --
GO:0007049 cell cycle IEA --
GO:0016310 phosphorylation IEA --
GO:0030010 establishment of cell polarity IEA --
genes like me logo Genes that share ontologies with NEK3: view

No data available for SIGNOR curated interactions for NEK3 Gene

Drugs & Compounds for NEK3 Gene

(6) Drugs for NEK3 Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
Magnesium Approved, Experimental, Investigational Pharma 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Pore Blocker, Potentiation 0

(3) Additional Compounds for NEK3 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Full agonist, Agonist, Partial agonist, Antagonist, Gating inhibitor 58-64-0
genes like me logo Genes that share compounds with NEK3: view

Transcripts for NEK3 Gene

mRNA/cDNA for NEK3 Gene

3 REFSEQ mRNAs :
19 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NEK3

Clone Products

  • Addgene plasmids for NEK3

Alternative Splicing Database (ASD) splice patterns (SP) for NEK3 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b
SP1: - -
SP2: -
SP3: -
SP4: - -
SP5: -

Relevant External Links for NEK3 Gene

GeneLoc Exon Structure for
NEK3

Expression for NEK3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NEK3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NEK3 Gene

This gene is overexpressed in Testis (65.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NEK3 Gene



Protein tissue co-expression partners for NEK3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NEK3

SOURCE GeneReport for Unigene cluster for NEK3 Gene:

Hs.409989

mRNA Expression by UniProt/SwissProt for NEK3 Gene:

P51956-NEK3_HUMAN
Tissue specificity: Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.

Evidence on tissue expression from TISSUES for NEK3 Gene

  • Intestine(4.3)
  • Liver(4.1)
genes like me logo Genes that share expression patterns with NEK3: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NEK3 Gene

Orthologs for NEK3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NEK3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NEK3 31 30
  • 99.87 (n)
OneToOne
cow
(Bos Taurus)
Mammalia NEK3 31 30
  • 87.11 (n)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 82 (a)
OneToMany
-- 31
  • 73 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Nek3 30
  • 79.96 (n)
mouse
(Mus musculus)
Mammalia Nek3 17 31 30
  • 79.45 (n)
chicken
(Gallus gallus)
Aves NEK3 31 30
  • 68.44 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NEK3 31
  • 64 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nek3 30
  • 61.8 (n)
Str.19964 30
zebrafish
(Danio rerio)
Actinopterygii LOC100536894 30
  • 68.68 (n)
NEK3 31
  • 67 (a)
OneToOne
thale cress
(Arabidopsis thaliana)
eudicotyledons NEK5 30
  • 47.95 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 52 (a)
OneToMany
Species where no ortholog for NEK3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • dog (Canis familiaris)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NEK3 Gene

ENSEMBL:
Gene Tree for NEK3 (if available)
TreeFam:
Gene Tree for NEK3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NEK3: view image

Paralogs for NEK3 Gene

Paralogs for NEK3 Gene

(61) SIMAP similar genes for NEK3 Gene using alignment to 7 proteins:

  • NEK3_HUMAN
  • F8VTT4_HUMAN
  • F8VV00_HUMAN
  • F8VXS4_HUMAN
  • G3V202_HUMAN
  • J3KPF4_HUMAN
  • Q6ZN64_HUMAN
genes like me logo Genes that share paralogs with NEK3: view

Variants for NEK3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NEK3 Gene

SNP ID Clinical significance and condition Chr 13 pos Variation AA Info Type
rs17482764 - p.His23Leu
rs34076988 - p.Asp461Asn
rs34077016 - p.Arg259Gly
rs34488913 - p.Glu477Lys
rs55946204 - p.Pro60Arg

Additional dbSNP identifiers (rs#s) for NEK3 Gene

Structural Variations from Database of Genomic Variants (DGV) for NEK3 Gene

Variant ID Type Subtype PubMed ID
nsv1037567 CNV gain 25217958
nsv428288 CNV loss 18775914

Variation tolerance for NEK3 Gene

Residual Variation Intolerance Score: 91.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 12.71; 94.65% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NEK3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NEK3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NEK3 Gene

Disorders for NEK3 Gene

MalaCards: The human disease database

(6) MalaCards diseases for NEK3 Gene - From: DISEASES, Novoseek, and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search NEK3 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NEK3

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with NEK3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NEK3 Gene

Publications for NEK3 Gene

  1. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. (PMID: 7522034) Schultz SJ … Nigg EA (Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1994) 2 3 4 23 54
  2. Novel association of Vav2 and Nek3 modulates signaling through the human prolactin receptor. (PMID: 15618286) Miller SL … Clevenger CV (Molecular endocrinology (Baltimore, Md.) 2005) 3 4 23 54
  3. Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). (PMID: 12063396) Kimura M … Okano Y (Cytogenetics and cell genetics 2001) 3 4 23 54
  4. Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans. (PMID: 8274451) Schultz SJ … Nigg EA (Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1993) 2 3 4 54
  5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41 54

Products for NEK3 Gene

Sources for NEK3 Gene