NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008] See more...

Aliases for NEIL3 Gene

Aliases for NEIL3 Gene

  • Nei Like DNA Glycosylase 3 2 3 5
  • Zinc Finger, GRF-Type Containing 3 2 3
  • DNA Glycosylase/AP Lyase Neil3 3 4
  • Endonuclease VIII-Like 3 3 4
  • Endonuclease 8-Like 3 3 4
  • DNA Glycosylase FPG2 3 4
  • Nei-Like Protein 3 3 4
  • Nei Endonuclease VIII-Like 3 (E. Coli) 2
  • Nei Endonuclease VIII-Like 3 3
  • DNA Glycosylase HFPG2 3
  • EC 4.2.99.18 4
  • EC 3.2.2.- 4
  • ZGRF3 3
  • HFPG2 3
  • HNEI3 3
  • FGP2 3
  • FPG2 3
  • NEI3 3

External Ids for NEIL3 Gene

Previous GeneCards Identifiers for NEIL3 Gene

  • GC04P178607
  • GC04P178468
  • GC04P178230
  • GC04P173981

Summaries for NEIL3 Gene

Entrez Gene Summary for NEIL3 Gene

  • NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]

GeneCards Summary for NEIL3 Gene

NEIL3 (Nei Like DNA Glycosylase 3) is a Protein Coding gene. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and single-stranded DNA binding. An important paralog of this gene is NEIL2.

UniProtKB/Swiss-Prot Summary for NEIL3 Gene

  • DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.

Additional gene information for NEIL3 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NEIL3 Gene

Genomics for NEIL3 Gene

GeneHancer (GH) Regulatory Elements for NEIL3 Gene

Promoters and enhancers for NEIL3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH04J177308 Promoter/Enhancer 2.4 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 500.7 +0.0 21 3.5 SIN3A TCF12 NRF1 POLR2G SP1 PHF8 FOS ZFX ZIC2 POLR2A NEIL3 ENSG00000249008 ENSG00000249084
GH04J177339 Enhancer 0.9 Ensembl ENCODE dbSUPER 11.3 +30.0 29972 0.4 CTCF REST RAD21 TRIM22 SMC3 NR2C1 NR2F2 NR2F1 ZNF654 EGR1 ENSG00000249008 NEIL3 ENSG00000249084
GH04J177270 Enhancer 0.6 Ensembl ENCODE 11.6 -38.0 -37988 1.9 ZNF316 ATF4 MAFK REST CBFA2T3 EMSY NEIL3 ENSG00000249008 ENSG00000287544
GH04J177281 Enhancer 0.6 Ensembl ENCODE 12.4 -28.3 -28336 1.8 NR2F2 CEBPB ZNF384 GATA2 NEIL3 ENSG00000249008 ENSG00000287544
GH04J177265 Enhancer 0.5 ENCODE 11 -43.5 -43472 1.8 NCOR1 SKI SMAD4 HNF4A NFIL3 HLF TBL1XR1 BHLHE40 GABPA CEBPA NEIL3 ENSG00000249008 ENSG00000287544
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NEIL3 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NEIL3

Top Transcription factor binding sites by QIAGEN in the NEIL3 gene promoter:
  • CUTL1
  • Evi-1
  • GATA-1
  • HNF-3beta
  • IRF-2
  • Nkx6-1
  • POU3F2
  • POU3F2 (N-Oct-5a)
  • POU3F2 (N-Oct-5b)
  • RSRFC4

Genomic Locations for NEIL3 Gene

Genomic Locations for NEIL3 Gene
chr4:177,309,837-177,362,938
(GRCh38/hg38)
Size:
53,102 bases
Orientation:
Plus strand
chr4:178,230,990-178,284,097
(GRCh37/hg19)
Size:
53,108 bases
Orientation:
Plus strand

Genomic View for NEIL3 Gene

Genes around NEIL3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEIL3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEIL3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEIL3 Gene

Proteins for NEIL3 Gene

  • Protein details for NEIL3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8TAT5-NEIL3_HUMAN
    Recommended name:
    Endonuclease 8-like 3
    Protein Accession:
    Q8TAT5
    Secondary Accessions:
    • Q2PPJ3
    • Q8NG51
    • Q9NV95

    Protein attributes for NEIL3 Gene

    Size:
    605 amino acids
    Molecular mass:
    67769 Da
    Quaternary structure:
    No Data Available

neXtProt entry for NEIL3 Gene

Post-translational modifications for NEIL3 Gene

  • Ubiquitination at Lys13, Lys110, Lys195, Lys316, Lys372, Lys398, Lys558, Lys574, and Lys585
  • Modification sites at PhosphoSitePlus

Other Protein References for NEIL3 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for NEIL3 Gene

Domains & Families for NEIL3 Gene

Gene Families for NEIL3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for NEIL3 Gene

GenScript: Design optimal peptide antigens:
  • Nei-like protein 3 (NEIL3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8TAT5

UniProtKB/Swiss-Prot:

NEIL3_HUMAN :
  • The N-terminal region (2-281) contains the glycosylase and lyase activities.
  • Belongs to the FPG family.
Domain:
  • The N-terminal region (2-281) contains the glycosylase and lyase activities.
Family:
  • Belongs to the FPG family.
genes like me logo Genes that share domains with NEIL3: view

Function for NEIL3 Gene

Molecular function for NEIL3 Gene

UniProtKB/Swiss-Prot Function:
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.; EC=4.2.99.18; Evidence=. ;.

Enzyme Numbers (IUBMB) for NEIL3 Gene

Phenotypes From GWAS Catalog for NEIL3 Gene

Gene Ontology (GO) - Molecular Function for NEIL3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000405 bubble DNA binding ISS --
GO:0003676 nucleic acid binding IEA --
GO:0003677 DNA binding IEA --
GO:0003684 damaged DNA binding IEA --
GO:0003690 double-stranded DNA binding IEA,ISS --
genes like me logo Genes that share ontologies with NEIL3: view
genes like me logo Genes that share phenotypes with NEIL3: view

Animal Models for NEIL3 Gene

MGI Knock Outs for NEIL3:

Animal Model Products

miRNA for NEIL3 Gene

miRTarBase miRNAs that target NEIL3

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NEIL3 Gene

Localization for NEIL3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEIL3 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NEIL3 gene
Compartment Confidence
nucleus 5
mitochondrion 2
cytosol 2
plasma membrane 1
extracellular 1
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for NEIL3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA,IEA 12433996
GO:0005654 nucleoplasm IDA --
genes like me logo Genes that share ontologies with NEIL3: view

Pathways & Interactions for NEIL3 Gene

PathCards logo

SuperPathways for NEIL3 Gene

genes like me logo Genes that share pathways with NEIL3: view

Pathways by source for NEIL3 Gene

1 KEGG pathway for NEIL3 Gene

Gene Ontology (GO) - Biological Process for NEIL3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006281 DNA repair IEA --
GO:0006284 base-excision repair ISS,IDA --
GO:0006289 nucleotide-excision repair IEA --
GO:0006974 cellular response to DNA damage stimulus IEA --
GO:0008152 metabolic process IEA --
genes like me logo Genes that share ontologies with NEIL3: view

No data available for SIGNOR curated interactions for NEIL3 Gene

Drugs & Compounds for NEIL3 Gene

No Compound Related Data Available

Transcripts for NEIL3 Gene

mRNA/cDNA for NEIL3 Gene

1 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NEIL3 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11
SP1: -
SP2:
SP3: -

Relevant External Links for NEIL3 Gene

GeneLoc Exon Structure for
NEIL3

Expression for NEIL3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NEIL3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NEIL3 Gene

This gene is overexpressed in Esophagus - Mucosa (x5.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NEIL3 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NEIL3

SOURCE GeneReport for Unigene cluster for NEIL3 Gene:

Hs.405467

mRNA Expression by UniProt/SwissProt for NEIL3 Gene:

Q8TAT5-NEIL3_HUMAN
Tissue specificity: Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.

Evidence on tissue expression from TISSUES for NEIL3 Gene

  • Skin(4)
genes like me logo Genes that share expression patterns with NEIL3: view

No data available for Protein differential expression in normal tissues , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for NEIL3 Gene

Orthologs for NEIL3 Gene

This gene was present in the common ancestor of chordates.

Orthologs for NEIL3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NEIL3 31 30
  • 99.01 (n)
OneToOne
cow
(Bos Taurus)
Mammalia NEIL3 31 30
  • 85.26 (n)
OneToOne
dog
(Canis familiaris)
Mammalia NEIL3 31 30
  • 83.8 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Neil3 17 31 30
  • 80.5 (n)
rat
(Rattus norvegicus)
Mammalia Neil3 30
  • 80 (n)
oppossum
(Monodelphis domestica)
Mammalia NEIL3 31
  • 62 (a)
OneToOne
chicken
(Gallus gallus)
Aves NEIL3 31 30
  • 64.82 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NEIL3 31
  • 55 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia neil3 30
  • 62.25 (n)
Str.6541 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.10504 30
zebrafish
(Danio rerio)
Actinopterygii neil3 31 30
  • 59.16 (n)
OneToMany
Dr.17827 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.3316 30
Species where no ortholog for NEIL3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NEIL3 Gene

ENSEMBL:
Gene Tree for NEIL3 (if available)
TreeFam:
Gene Tree for NEIL3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NEIL3: view image

Paralogs for NEIL3 Gene

Paralogs for NEIL3 Gene

genes like me logo Genes that share paralogs with NEIL3: view

Variants for NEIL3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NEIL3 Gene

SNP ID Clinical significance and condition Chr 04 pos Variation AA Info Type
709896 Likely Benign: not provided 177,310,022(+) G/A SYNONYMOUS_VARIANT
rs13112358 - p.Pro443Leu
rs13112390 - p.Gln471His
rs17064658 - p.Gln172His
rs17064676 - p.Ile346Val

Additional dbSNP identifiers (rs#s) for NEIL3 Gene

Structural Variations from Database of Genomic Variants (DGV) for NEIL3 Gene

Variant ID Type Subtype PubMed ID
esv2728772 CNV deletion 23290073
esv2759304 CNV gain 17122850
esv34846 CNV gain 17911159
esv3569839 CNV loss 25503493
esv3603300 CNV gain 21293372
esv3894076 CNV loss 25118596
nsv508336 CNV deletion 20534489
nsv822852 CNV gain 20364138

Variation tolerance for NEIL3 Gene

Residual Variation Intolerance Score: 93.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.35; 85.37% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NEIL3 Gene

Human Gene Mutation Database (HGMD)
NEIL3
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NEIL3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NEIL3 Gene

Disorders for NEIL3 Gene

Additional Disease Information for NEIL3

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for NEIL3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NEIL3 Gene

Publications for NEIL3 Gene

  1. Human Nei-like protein NEIL3 has AP lyase activity specific for single-stranded DNA and confers oxidative stress resistance in Escherichia coli mutant. (PMID: 19170771) Takao M … Zhang QM (Genes to cells : devoted to molecular & cellular mechanisms 2009) 2 3 4 23 54
  2. Human NEIL3 is mainly a monofunctional DNA glycosylase removing spiroimindiohydantoin and guanidinohydantoin. (PMID: 23755964) Krokeide SZ … Bjørås M (DNA repair 2013) 2 3 4 54
  3. Expression and purification of NEIL3, a human DNA glycosylase homolog. (PMID: 19121397) Krokeide SZ … Luna L (Protein expression and purification 2009) 3 4 23 54
  4. The novel DNA glycosylase, NEIL1, protects mammalian cells from radiation-mediated cell death. (PMID: 12713815) Rosenquist TA … Grollman AP (DNA repair 2003) 2 3 23 54
  5. Human DNA glycosylases of the bacterial Fpg/MutM superfamily: an alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA. (PMID: 12433996) Morland I … Seeberg E (Nucleic acids research 2002) 3 4 23 54

Products for NEIL3 Gene

  • Signalway ELISA kits for NEIL3
  • Signalway Proteins for NEIL3

Sources for NEIL3 Gene