This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in ... See more...

Aliases for NCK2 Gene

Aliases for NCK2 Gene

  • NCK Adaptor Protein 2 2 3 4 5
  • Growth Factor Receptor-Bound Protein 4 3 4
  • SH2/SH3 Adaptor Protein NCK-Beta 3 4
  • Cytoplasmic Protein NCK2 3 4
  • GRB4 3 4
  • Noncatalytic Region Of Tyrosine Kinase, Beta 3
  • NCKbeta 3
  • Nck-2 4

External Ids for NCK2 Gene

Previous GeneCards Identifiers for NCK2 Gene

  • GC02P104297
  • GC02P104885
  • GC02P106037
  • GC02P106054
  • GC02P105926
  • GC02P105727
  • GC02P106361
  • GC02P100201

Summaries for NCK2 Gene

Entrez Gene Summary for NCK2 Gene

  • This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

GeneCards Summary for NCK2 Gene

NCK2 (NCK Adaptor Protein 2) is a Protein Coding gene. Among its related pathways are Signaling by Robo receptor and IL-2 Pathway. Gene Ontology (GO) annotations related to this gene include SH3/SH2 adaptor activity and cytoskeletal adaptor activity. An important paralog of this gene is NCK1.

UniProtKB/Swiss-Prot Summary for NCK2 Gene

  • Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Gene Wiki entry for NCK2 Gene

Additional gene information for NCK2 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NCK2 Gene

Genomics for NCK2 Gene

GeneHancer (GH) Regulatory Elements for NCK2 Gene

Promoters and enhancers for NCK2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH02J105851 Promoter/Enhancer 1.3 EPDnew ENCODE dbSUPER 507.4 +107.2 107191 0.8 MYC TAF9B RBM25 MBD2 NEUROD1 POLR2A GABPA POLR2H TBP PBX2 NCK2 ENSG00000235522 HSALNG0017603
GH02J105853 Promoter/Enhancer 1.2 EPDnew ENCODE dbSUPER 500.1 +108.8 108822 1.2 ZNF217 CUX1 CTBP1 SIN3A MBD2 NR3C1 NEUROD1 ELF1 GATA3 MYC NCK2 ENSG00000235522 HSALNG0017603
GH02J105794 Enhancer 1.4 FANTOM5 ENCODE dbSUPER 23.6 +55.0 55001 10.2 ZSCAN5C SP1 NCOR1 EP300 RELA MEF2C ZBTB25 MYC TGIF2 CTBP1 HSALNG0017603 NCK2 ENSG00000235522 TGFBRAP1 C2orf49
GH02J105743 Promoter/Enhancer 2.1 FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 9.1 +2.3 2296 7.5 NRF1 PHF8 ZFX POLR2A ZNF263 ZBTB26 SAP30 RELA TGIF2 RAD21 ENSG00000235522 ENSG00000238273 NCK2 PLGLA C2orf49 TGFBRAP1 HSALNG0017603
GH02J105788 Enhancer 1.1 FANTOM5 ENCODE dbSUPER 17.4 +45.4 45395 3.1 JUND KLF4 MEF2C NFYB BCL11A ZNF263 CTCF SMC3 FOS MEF2B NCK2 ENSG00000235522 TGFBRAP1 HSALNG0017603
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NCK2 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NCK2

Top Transcription factor binding sites by QIAGEN in the NCK2 gene promoter:
  • COMP1
  • E2F
  • E2F-1
  • E2F-2
  • E2F-3a
  • E2F-4
  • E2F-5
  • GATA-1
  • Nkx6-1
  • Pax-5

Genomic Locations for NCK2 Gene

Genomic Locations for NCK2 Gene
chr2:105,744,912-105,894,274
(GRCh38/hg38)
Size:
149,363 bases
Orientation:
Plus strand
chr2:106,361,354-106,510,730
(GRCh37/hg19)
Size:
149,377 bases
Orientation:
Plus strand

Genomic View for NCK2 Gene

Genes around NCK2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NCK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NCK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NCK2 Gene

Proteins for NCK2 Gene

  • Protein details for NCK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43639-NCK2_HUMAN
    Recommended name:
    Cytoplasmic protein NCK2
    Protein Accession:
    O43639
    Secondary Accessions:
    • D3DVK1
    • Q9BWN9
    • Q9UIC3

    Protein attributes for NCK2 Gene

    Size:
    380 amino acids
    Molecular mass:
    42915 Da
    Quaternary structure:
    • Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG (By similarity). Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1.

    Three dimensional structures from OCA and Proteopedia for NCK2 Gene

neXtProt entry for NCK2 Gene

Post-translational modifications for NCK2 Gene

No data available for DME Specific Peptides for NCK2 Gene

Domains & Families for NCK2 Gene

Gene Families for NCK2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for NCK2 Gene

Blocks:
  • SH3 domain signature
  • SH2 domain signature
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for NCK2 Gene

GenScript: Design optimal peptide antigens:
  • SH2/SH3 adaptor protein NCK-beta (NCK2_HUMAN)
genes like me logo Genes that share domains with NCK2: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for NCK2 Gene

Function for NCK2 Gene

Molecular function for NCK2 Gene

UniProtKB/Swiss-Prot Function:
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
GENATLAS Biochemistry:
transduction adaptator protein 2 interacting with EGFR and PDGFR

Phenotypes From GWAS Catalog for NCK2 Gene

Gene Ontology (GO) - Molecular Function for NCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001784 phosphotyrosine residue binding IPI 20624904
GO:0005070 SH3/SH2 adaptor activity NAS 16835242
GO:0005515 protein binding IEA,IPI 10026169
GO:0008093 cytoskeletal adaptor activity NAS 12110186
GO:0030159 receptor signaling complex scaffold activity NAS 12110186
genes like me logo Genes that share ontologies with NCK2: view
genes like me logo Genes that share phenotypes with NCK2: view

Animal Models for NCK2 Gene

MGI Knock Outs for NCK2:

Animal Model Products

CRISPR Products

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NCK2 Gene

Localization for NCK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NCK2 Gene

Cytoplasm. Endoplasmic reticulum.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NCK2 gene
Compartment Confidence
cytosol 5
endoplasmic reticulum 4
nucleus 3
plasma membrane 2
extracellular 2
cytoskeleton 2
mitochondrion 1
endosome 1
lysosome 1
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for NCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm NAS 12110186
GO:0005783 endoplasmic reticulum IDA 16835242
GO:0005829 cytosol TAS --
GO:0012506 vesicle membrane IEA --
GO:0014069 postsynaptic density IEA --
genes like me logo Genes that share ontologies with NCK2: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for NCK2 Gene

Pathways & Interactions for NCK2 Gene

PathCards logo

SuperPathways for NCK2 Gene

SuperPathway Contained pathways
1 RET signaling
2 EphB-EphrinB Signaling
.58
.58
.33
3 EPH-Ephrin signaling
.35
4 Signaling by Robo receptor
5 Developmental Biology
genes like me logo Genes that share pathways with NCK2: view

Pathways by source for NCK2 Gene

3 GeneGo (Thomson Reuters) pathways for NCK2 Gene
  • Cell adhesion Ephrins signaling
  • Cytoskeleton remodeling Integrin outside-in signaling
  • Cytoskeleton remodeling Reverse signaling by ephrin B
13 Qiagen pathways for NCK2 Gene
  • Actin Nucleation by ARP-WASP Complex
  • Angiopoietin-TIE2 Signaling
  • Caspase Cascade
  • EGF Pathway
  • EphB-EphrinB Signaling

SIGNOR curated interactions for NCK2 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for NCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001771 immunological synapse formation IEA --
GO:0006417 regulation of translation IEA --
GO:0007015 actin filament organization IEA --
GO:0007165 signal transduction TAS 9737977
GO:0007172 signal complex assembly NAS 12110186
genes like me logo Genes that share ontologies with NCK2: view

Drugs & Compounds for NCK2 Gene

(1) Drugs for NCK2 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with NCK2: view

Transcripts for NCK2 Gene

mRNA/cDNA for NCK2 Gene

3 REFSEQ mRNAs :
13 NCBI additional mRNA sequence :
6 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NCK2 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b
SP1:
SP2: -

Relevant External Links for NCK2 Gene

GeneLoc Exon Structure for
NCK2

Expression for NCK2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NCK2 Gene

Protein differential expression in normal tissues from HIPED for NCK2 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (35.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NCK2 Gene



Protein tissue co-expression partners for NCK2 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NCK2

SOURCE GeneReport for Unigene cluster for NCK2 Gene:

Hs.529244

mRNA Expression by UniProt/SwissProt for NCK2 Gene:

O43639-NCK2_HUMAN
Tissue specificity: Ubiquitous.

Evidence on tissue expression from TISSUES for NCK2 Gene

  • Blood(4.3)
  • Lung(4.2)
  • Skin(3.8)
  • Nervous system(2.5)
genes like me logo Genes that share expression patterns with NCK2: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NCK2 Gene

Orthologs for NCK2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NCK2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NCK2 31 30
  • 99.65 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NCK2 31
  • 92 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NCK2 31 30
  • 91.49 (n)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NCK2 31
  • 90 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nck2 30
  • 88.68 (n)
mouse
(Mus musculus)
Mammalia Nck2 17 31 30
  • 88.6 (n)
cow
(Bos Taurus)
Mammalia NCK2 31 30
  • 85.2 (n)
OneToOne
chicken
(Gallus gallus)
Aves NCK2 31 30
  • 78.33 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NCK2 31
  • 92 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nck2 30
  • 71.32 (n)
zebrafish
(Danio rerio)
Actinopterygii nck2a 31
  • 77 (a)
OneToMany
nck2b 31 30
  • 74.36 (n)
OneToMany
-- 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13508 30
fruit fly
(Drosophila melanogaster)
Insecta dock 31 32
  • 32 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea nck-1 31 30
  • 51.06 (n)
OneToMany
ZK470.5b 32
  • 42 (a)
ZK470.5a 32
  • 41 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 39 (a)
OneToMany
Species where no ortholog for NCK2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NCK2 Gene

ENSEMBL:
Gene Tree for NCK2 (if available)
TreeFam:
Gene Tree for NCK2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NCK2: view image

Paralogs for NCK2 Gene

Paralogs for NCK2 Gene

(19) SIMAP similar genes for NCK2 Gene using alignment to 6 proteins:

  • NCK2_HUMAN
  • E7EPF5_HUMAN
  • E7ERP6_HUMAN
  • E9PEE2_HUMAN
  • Q53T91_HUMAN
  • Q53TG4_HUMAN
genes like me logo Genes that share paralogs with NCK2: view

Variants for NCK2 Gene

Additional dbSNP identifiers (rs#s) for NCK2 Gene

Structural Variations from Database of Genomic Variants (DGV) for NCK2 Gene

Variant ID Type Subtype PubMed ID
dgv1159e212 CNV loss 25503493
dgv1985n106 CNV deletion 24896259
esv22511 CNV gain 19812545
esv24019 CNV loss 19812545
esv2478624 CNV insertion 19546169
esv2668709 CNV deletion 23128226
esv2676187 CNV deletion 23128226
esv26794 CNV gain 19812545
esv2762748 CNV loss 21179565
esv3375455 CNV insertion 20981092
esv3437222 CNV insertion 20981092
esv3559896 CNV deletion 23714750
esv3584000 CNV loss 25503493
esv3591832 CNV loss 21293372
esv3591838 CNV loss 21293372
esv3591839 CNV loss 21293372
esv3591840 CNV gain 21293372
esv3591841 CNV loss 21293372
esv3591842 CNV loss 21293372
esv3591843 CNV loss 21293372
esv3591844 CNV loss 21293372
esv3591846 CNV loss 21293372
esv3591847 CNV gain 21293372
esv994957 OTHER inversion 20482838
nsv10108 CNV loss 18304495
nsv1072962 CNV deletion 25765185
nsv1114122 CNV deletion 24896259
nsv834321 CNV loss 17160897
nsv953501 CNV duplication 24416366

Variation tolerance for NCK2 Gene

Residual Variation Intolerance Score: 9.73% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.68; 14.53% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NCK2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NCK2

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NCK2 Gene

Disorders for NCK2 Gene

Additional Disease Information for NCK2

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for NCK2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NCK2 Gene

Publications for NCK2 Gene

  1. Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck. (PMID: 10026169) Braverman LE … Quilliam LA (The Journal of biological chemistry 1999) 3 4 23 54
  2. Identification of Nck family genes, chromosomal localization, expression, and signaling specificity. (PMID: 9737977) Chen M … Li W (The Journal of biological chemistry 1998) 2 3 4 54
  3. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41 54
  4. Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening. (PMID: 19807924) Voss M … Janssen O (BMC immunology 2009) 3 4 54
  5. Pinpointing phosphotyrosine-dependent interactions downstream of the collagen receptor DDR1. (PMID: 16337946) Koo DH … Vogel WF (FEBS letters 2006) 3 4 54

Products for NCK2 Gene

Sources for NCK2 Gene