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Aliases for NCK1 Gene

Aliases for NCK1 Gene

  • NCK Adaptor Protein 1 2 3 4 5
  • SH2/SH3 Adaptor Protein NCK-Alpha 3 4
  • Nck-1 3 4
  • NCK 3 4
  • Non-Catalytic Region Of Tyrosine Kinase 3
  • Cytoplasmic Protein NCK1 3
  • Melanoma NCK Protein 3
  • NCK Tyrosine Kinase 3
  • NCKalpha 3

External Ids for NCK1 Gene

Previous HGNC Symbols for NCK1 Gene

  • NCK

Previous GeneCards Identifiers for NCK1 Gene

  • GC03P133543
  • GC03P137332
  • GC03P137862
  • GC03P137901
  • GC03P138063
  • GC03P136581
  • GC03P133957

Summaries for NCK1 Gene

Entrez Gene Summary for NCK1 Gene

  • The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

GeneCards Summary for NCK1 Gene

NCK1 (NCK Adaptor Protein 1) is a Protein Coding gene. Diseases associated with NCK1 include Wiskott-Aldrich Syndrome and Multiple Endocrine Neoplasia, Type Iib. Among its related pathways are Primary Focal Segmental Glomerulosclerosis FSGS and Beta-Adrenergic Signaling. Gene Ontology (GO) annotations related to this gene include receptor binding and protein binding, bridging. An important paralog of this gene is NCK2.

UniProtKB/Swiss-Prot for NCK1 Gene

  • Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

Gene Wiki entry for NCK1 Gene

Additional gene information for NCK1 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NCK1 Gene

Genomics for NCK1 Gene

GeneHancer (GH) Regulatory Elements for NCK1 Gene

Promoters and enhancers for NCK1 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH03I136860 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 571.2 -0.1 -67 3.3 HDGF PKNOX1 SMAD1 ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 SLC30A9 NCK1 NCK1-AS1 STAG1 IL20RB MSL2 SLC35G2 ENSG00000273486 ENSG00000242968
GH03I136929 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE 550.2 +68.4 68359 1.9 ZNF664 MAX USF2 CEBPB NCK1 ENSG00000273486 NCK1-AS1 IL20RB RAD51AP1P1 GC03P136873
GH03I136892 Promoter/Enhancer 1.2 EPDnew Ensembl ENCODE 562.5 +31.5 31482 3.1 GATA3 FOXA2 POLR2A JUN CEBPB CBFA2T3 FOS NCK1 IL20RB NCK1-AS1 ENSG00000273486 RAD51AP1P1 GC03P136873
GH03I136191 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 10.9 -667.2 -667192 7.1 CLOCK MLX DMAP1 YY1 SLC30A9 ZNF143 SP3 MEF2D ZNF610 GLIS1 MSL2 PCCB NCK1 IL20RB NCK1-AS1 GC03P136156
GH03I136183 Enhancer 0.7 Ensembl 22.1 -680.5 -680507 0.2 ZNF189 ZNF529 ZNF140 ZNF266 ZC3H11A ZNF501 ZNF404 ZNF664 ZNF213 ZNF354C MSL2 STAG1 NCK1 SLC35G2 NCK1-AS1 PCCB GC03P136156
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around NCK1 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the NCK1 gene promoter:

Genomic Locations for NCK1 Gene

Genomic Locations for NCK1 Gene
chr3:136,862,208-136,951,610
(GRCh38/hg38)
Size:
89,403 bases
Orientation:
Plus strand
chr3:136,581,050-136,668,665
(GRCh37/hg19)

Genomic View for NCK1 Gene

Genes around NCK1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NCK1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NCK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NCK1 Gene

Proteins for NCK1 Gene

  • Protein details for NCK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P16333-NCK1_HUMAN
    Recommended name:
    Cytoplasmic protein NCK1
    Protein Accession:
    P16333
    Secondary Accessions:
    • B7Z751
    • D3DNE3

    Protein attributes for NCK1 Gene

    Size:
    377 amino acids
    Molecular mass:
    42864 Da
    Quaternary structure:
    • Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2. Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15. Interacts with FASLG. Directly interacts with RASA1. Interacts with isoform 4 of MINK1. Interacts with FLT1 (tyrosine phosphorylated). Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion. Interacts with EPHA2. Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated). Interacts with the inactive form of EIF2AK2/PKR.

    Three dimensional structures from OCA and Proteopedia for NCK1 Gene

    Alternative splice isoforms for NCK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NCK1 Gene

Post-translational modifications for NCK1 Gene

  • Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for NCK1 Gene

Domains & Families for NCK1 Gene

Gene Families for NCK1 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for NCK1 Gene

Graphical View of Domain Structure for InterPro Entry

P16333

UniProtKB/Swiss-Prot:

NCK1_HUMAN :
  • Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seeem to be involved.
Domain:
  • Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seeem to be involved.
genes like me logo Genes that share domains with NCK1: view

Function for NCK1 Gene

Molecular function for NCK1 Gene

GENATLAS Biochemistry:
transduction adaptor protein 1
UniProtKB/Swiss-Prot Function:
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

Phenotypes From GWAS Catalog for NCK1 Gene

Gene Ontology (GO) - Molecular Function for NCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004860 protein kinase inhibitor activity IDA 18835251
GO:0005070 SH3/SH2 adaptor activity NAS 16835242
GO:0005102 receptor binding IPI 12110186
GO:0005515 protein binding IPI 9346925
GO:0008093 cytoskeletal adaptor activity NAS 12110186
genes like me logo Genes that share ontologies with NCK1: view
genes like me logo Genes that share phenotypes with NCK1: view

Animal Models for NCK1 Gene

MGI Knock Outs for NCK1:

Clone Products

  • Addgene plasmids for NCK1

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NCK1 Gene

Localization for NCK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NCK1 Gene

Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NCK1 gene
Compartment Confidence
plasma membrane 5
nucleus 5
endoplasmic reticulum 5
cytosol 5
cytoskeleton 2
extracellular 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (4)
  • Plasma membrane (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000164 protein phosphatase type 1 complex IDA 16835242
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm NAS,TAS 12110186
GO:0005783 endoplasmic reticulum IDA 16835242
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with NCK1: view

Pathways & Interactions for NCK1 Gene

genes like me logo Genes that share pathways with NCK1: view

SIGNOR curated interactions for NCK1 Gene

Activates:
Inactivates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for NCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006417 regulation of translation IEA --
GO:0006469 negative regulation of protein kinase activity IEA --
GO:0006930 substrate-dependent cell migration, cell extension IEA --
GO:0007015 actin filament organization IEA --
GO:0007172 signal complex assembly NAS 12110186
genes like me logo Genes that share ontologies with NCK1: view

Drugs & Compounds for NCK1 Gene

(5) Drugs for NCK1 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(6) Additional Compounds for NCK1 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with NCK1: view

Transcripts for NCK1 Gene

Unigene Clusters for NCK1 Gene

NCK adaptor protein 1:
Representative Sequences:

Clone Products

  • Addgene plasmids for NCK1

Alternative Splicing Database (ASD) splice patterns (SP) for NCK1 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b · 8c ^ 9
SP1: - - - - - -
SP2: - - - - -
SP3: - - - -
SP4: - - - - -
SP5: - - - -
SP6: - - - - -
SP7:
SP8:

Relevant External Links for NCK1 Gene

GeneLoc Exon Structure for
NCK1
ECgene alternative splicing isoforms for
NCK1

Expression for NCK1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NCK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NCK1 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (17.9), CD8 Tcells (7.6), and Lymph node (6.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NCK1 Gene



Protein tissue co-expression partners for NCK1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NCK1 Gene:

NCK1

SOURCE GeneReport for Unigene cluster for NCK1 Gene:

Hs.477693

Evidence on tissue expression from TISSUES for NCK1 Gene

  • Kidney(4.5)
  • Blood(4.4)
  • Liver(4.4)
  • Nervous system(3)
  • Heart(2.1)
genes like me logo Genes that share expression patterns with NCK1: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for NCK1 Gene

Orthologs for NCK1 Gene

This gene was present in the common ancestor of animals.

Orthologs for NCK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NCK1 33 34
  • 99.73 (n)
dog
(Canis familiaris)
Mammalia NCK1 34 33
  • 95.84 (n)
OneToOne
cow
(Bos Taurus)
Mammalia NCK1 33 34
  • 95.31 (n)
oppossum
(Monodelphis domestica)
Mammalia NCK1 34
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nck1 33 16 34
  • 92.84 (n)
rat
(Rattus norvegicus)
Mammalia Nck1 33
  • 92.66 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia NCK1 34
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nck1 33
  • 78.04 (n)
Str.18569 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.379 33
zebrafish
(Danio rerio)
Actinopterygii nck1b 33 34
  • 68.79 (n)
nck1a 34
  • 64 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010135 33
  • 54.41 (n)
fruit fly
(Drosophila melanogaster)
Insecta dock 35 33 34
  • 52.86 (n)
worm
(Caenorhabditis elegans)
Secernentea nck-1 34
  • 44 (a)
OneToMany
ZK470.5b 35
  • 40 (a)
ZK470.5a 35
  • 40 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 39 (a)
OneToMany
Species where no ortholog for NCK1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NCK1 Gene

ENSEMBL:
Gene Tree for NCK1 (if available)
TreeFam:
Gene Tree for NCK1 (if available)

Paralogs for NCK1 Gene

Paralogs for NCK1 Gene

(10) SIMAP similar genes for NCK1 Gene using alignment to 7 proteins:

genes like me logo Genes that share paralogs with NCK1: view

Variants for NCK1 Gene

Sequence variations from dbSNP and Humsavar for NCK1 Gene

SNP ID Clin Chr 03 pos Variation AA Info Type
rs1000042986 -- 136,927,231(+) C/T genic_upstream_transcript_variant, intron_variant
rs1000057310 -- 136,921,715(+) A/G genic_upstream_transcript_variant, intron_variant
rs1000102337 -- 136,884,377(+) G/A genic_upstream_transcript_variant, intron_variant
rs1000108288 -- 136,877,310(+) A/G genic_upstream_transcript_variant, intron_variant
rs1000111783 -- 136,893,147(+) AG/ genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for NCK1 Gene

Variant ID Type Subtype PubMed ID
esv2725958 CNV deletion 23290073
esv3397853 CNV insertion 20981092
esv3419922 CNV insertion 20981092
esv3569018 CNV loss 25503493
nsv1114425 CNV deletion 24896259

Variation tolerance for NCK1 Gene

Residual Variation Intolerance Score: 34.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.29; 53.16% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NCK1 Gene

Human Gene Mutation Database (HGMD)
NCK1
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NCK1

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NCK1 Gene

Disorders for NCK1 Gene

MalaCards: The human disease database

(3) MalaCards diseases for NCK1 Gene - From: HGMD, DISEASES, Novoseek, and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search NCK1 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NCK1

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with NCK1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NCK1 Gene

Publications for NCK1 Gene

  1. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. (PMID: 17803907) Kremer BE … Macara IG (Cell 2007) 3 4 22 58
  2. Src-induced phosphorylation of caveolin-2 on tyrosine 19. Phospho-caveolin-2 (Tyr(P)19) is localized near focal adhesions, remains associated with lipid rafts/caveolae, but no longer forms a high molecular mass hetero-oligomer with caveolin-1. (PMID: 12091389) Lee H … Lisanti MP (The Journal of biological chemistry 2002) 3 4 22 58
  3. Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral. (PMID: 10747847) Rebhun JF … Quilliam LA (The Journal of biological chemistry 2000) 3 4 22 58
  4. Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck. (PMID: 10026169) Braverman LE … Quilliam LA (The Journal of biological chemistry 1999) 3 4 22 58
  5. Identification of Nck family genes, chromosomal localization, expression, and signaling specificity. (PMID: 9737977) Chen M … Li W (The Journal of biological chemistry 1998) 2 3 22 58

Products for NCK1 Gene

  • Addgene plasmids for NCK1

Sources for NCK1 Gene

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