The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by R... See more...

Aliases for NANOG Gene

Aliases for NANOG Gene

  • Nanog Homeobox 2 3 5
  • Homeobox Transcription Factor Nanog 3 4
  • Homeobox Protein NANOG 3 4
  • Homeobox Transcription Factor Nanog-Delta 48 3
  • FLJ12581 2
  • FLJ40451 2
  • HNanog 4
  • NANOG 5

External Ids for NANOG Gene

Previous GeneCards Identifiers for NANOG Gene

  • GC12P007842
  • GC12P007833
  • GC12U900534
  • GC12P007941
  • GC12P007940

Summaries for NANOG Gene

Entrez Gene Summary for NANOG Gene

  • The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

GeneCards Summary for NANOG Gene

NANOG (Nanog Homeobox) is a Protein Coding gene. Diseases associated with NANOG include Teratocarcinoma and Germ Cell And Embryonal Cancer. Among its related pathways are Proteoglycans in cancer and Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways and Lineage-specific Markers. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and chromatin binding. An important paralog of this gene is NANOGP8.

UniProtKB/Swiss-Prot Summary for NANOG Gene

  • Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gene Wiki entry for NANOG Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NANOG Gene

Genomics for NANOG Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for NANOG Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NANOG on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for NANOG

Top Transcription factor binding sites by QIAGEN in the NANOG gene promoter:
  • AREB6
  • E4BP4
  • Gfi-1
  • Hlf
  • HNF-4alpha1
  • HNF-4alpha2
  • p53
  • POU2F1
  • POU2F1a
  • STAT3

Genomic Locations for NANOG Gene

Latest Assembly
chr12:7,787,794-7,799,146
(GRCh38/hg38)
Size:
11,353 bases
Orientation:
Plus strand

Previous Assembly
chr12:7,941,998-7,951,742
(GRCh37/hg19 by Entrez Gene)
Size:
9,745 bases
Orientation:
Plus strand

chr12:7,940,390-7,948,655
(GRCh37/hg19 by Ensembl)
Size:
8,266 bases
Orientation:
Plus strand

Genomic View for NANOG Gene

Genes around NANOG on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NANOG Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NANOG Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NANOG Gene

Proteins for NANOG Gene

  • Protein details for NANOG Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H9S0-NANOG_HUMAN
    Recommended name:
    Homeobox protein NANOG
    Protein Accession:
    Q9H9S0
    Secondary Accessions:
    • D3DUU4
    • Q2TTG0
    • Q6JZS5

    Protein attributes for NANOG Gene

    Size:
    305 amino acids
    Molecular mass:
    34620 Da
    Quaternary structure:
    • Interacts with SMAD1 (By similarity). Interacts with SALL4 (By similarity). Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity). Interacts with NSD2 (By similarity).
    Miscellaneous:
    • Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.

    Three dimensional structures from OCA and Proteopedia for NANOG Gene

    Alternative splice isoforms for NANOG Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NANOG Gene

Post-translational modifications for NANOG Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for NANOG Gene

Antibodies for research

No data available for DME Specific Peptides for NANOG Gene

Domains & Families for NANOG Gene

Gene Families for NANOG Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Transcription factors

Protein Domains for NANOG Gene

Suggested Antigen Peptide Sequences for NANOG Gene

GenScript: Design optimal peptide antigens:
  • Homeobox transcription factor Nanog (NANOG_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H9S0

UniProtKB/Swiss-Prot:

NANOG_HUMAN :
  • Belongs to the Nanog homeobox family.
Family:
  • Belongs to the Nanog homeobox family.
genes like me logo Genes that share domains with NANOG: view

Function for NANOG Gene

Molecular function for NANOG Gene

UniProtKB/Swiss-Prot Function:
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Phenotypes From GWAS Catalog for NANOG Gene

Gene Ontology (GO) - Molecular Function for NANOG Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000976 transcription regulatory region sequence-specific DNA binding IDA 25825768
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IDA 23708658
GO:0000978 RNA polymerase II proximal promoter sequence-specific DNA binding IBA 21873635
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific ISA --
GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific ISS --
genes like me logo Genes that share ontologies with NANOG: view
genes like me logo Genes that share phenotypes with NANOG: view

Animal Models for NANOG Gene

MGI Knock Outs for NANOG:
Targeted motifs for NANOG Gene
HOMER Transcription Factor Regulatory Elements motif NANOG
  • Consensus sequence: GGCCATTAAC Submotif: canonical Cell Type: mES GEO ID: GSE11724

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NANOG

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology and Transcription Factor Targets for NANOG Gene

Localization for NANOG Gene

Subcellular locations from UniProtKB/Swiss-Prot for NANOG Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NANOG gene
Compartment Confidence
nucleus 5
plasma membrane 3
extracellular 3
cytoskeleton 3
cytosol 3
mitochondrion 2
endoplasmic reticulum 2
endosome 2
lysosome 2
peroxisome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (3)
  • Vesicles (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NANOG Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000785 chromatin ISA --
GO:0005634 nucleus IC,IDA 18932205
GO:0005654 nucleoplasm TAS --
GO:0005730 nucleolus IDA 20458727
GO:0043231 intracellular membrane-bounded organelle IDA --
genes like me logo Genes that share ontologies with NANOG: view

Pathways & Interactions for NANOG Gene

genes like me logo Genes that share pathways with NANOG: view

Pathways by source for NANOG Gene

5 Qiagen pathways for NANOG Gene
  • Human Early Embryo Development
  • Human Embryonic Stem Cell Pluripotency
  • Nanog in Mammalian ESC Pluripotency
  • Oct4 in Mammalian ESC Pluripotency
  • Transcriptional Regulatory Network in Embryonic Stem Cell
1 Cell Signaling Technology pathway for NANOG Gene

SIGNOR curated interactions for NANOG Gene

Activates:
Inactivates:
Is activated by:

Gene Ontology (GO) - Biological Process for NANOG Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II IEA --
GO:0001714 endodermal cell fate specification IDA 20713518
GO:0006355 regulation of transcription, DNA-templated IDA 16153702
GO:0006357 regulation of transcription by RNA polymerase II IBA 21873635
GO:0007275 multicellular organism development IEA --
genes like me logo Genes that share ontologies with NANOG: view

Drugs & Compounds for NANOG Gene

No Compound Related Data Available

Transcripts for NANOG Gene

mRNA/cDNA for NANOG Gene

2 REFSEQ mRNAs :
7 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NANOG

Alternative Splicing Database (ASD) splice patterns (SP) for NANOG Gene

No ASD Table

Relevant External Links for NANOG Gene

GeneLoc Exon Structure for
NANOG

Expression for NANOG Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NANOG Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NANOG Gene

This gene is overexpressed in Testis (x5.7).

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for NANOG

SOURCE GeneReport for Unigene cluster for NANOG Gene:

Hs.635882

mRNA Expression by UniProt/SwissProt for NANOG Gene:

Q9H9S0-NANOG_HUMAN
Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Evidence on tissue expression from TISSUES for NANOG Gene

  • Nervous system(2.8)
  • Skin(2.7)
  • Liver(2.7)
  • Muscle(2.6)
  • Blood(2.5)
  • Heart(2.4)
  • Kidney(2.4)
  • Bone marrow(2.4)
  • Intestine(2.4)
  • Lung(2.3)
  • Bone(2.3)
  • Pancreas(2.2)
genes like me logo Genes that share expression patterns with NANOG: view

Primer products for research

No data available for Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for NANOG Gene

Orthologs for NANOG Gene

This gene was present in the common ancestor of chordates.

Orthologs for NANOG Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia NANOG 29 30
  • 99.23 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia NANOG 29 30
  • 79.59 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia NANOG 29 30
  • 78.93 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Nanog 29
  • 73.97 (n)
Mouse
(Mus musculus)
Mammalia Nanog 29 16 30
  • 73.33 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia NANOG 30
  • 45 (a)
OneToOne
Chicken
(Gallus gallus)
Aves -- 30
  • 26 (a)
OneToMany
NANOG 30
  • 23 (a)
OneToMany
Lizard
(Anolis carolinensis)
Reptilia NANOG 30
  • 23 (a)
OneToOne
Species where no ortholog for NANOG was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)
  • Zebrafish (Danio rerio)

Evolution for NANOG Gene

ENSEMBL:
Gene Tree for NANOG (if available)
TreeFam:
Gene Tree for NANOG (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NANOG: view image
Alliance of Genome Resources:
Additional Orthologs for NANOG

Paralogs for NANOG Gene

Paralogs for NANOG Gene

(8) SIMAP similar genes for NANOG Gene using alignment to 7 proteins:

  • NANOG_HUMAN
  • E9PL07_HUMAN
  • F5GZI2_HUMAN
  • J7H4A9_HUMAN
  • J7H4F6_HUMAN
  • J7HA22_HUMAN
  • J7HA98_HUMAN
genes like me logo Genes that share paralogs with NANOG: view

Variants for NANOG Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for NANOG Gene

SNP ID Clinical significance and condition Chr 12 pos Variation AA Info Type
rs140243232 Likely Benign: not provided 7,789,729(+) C/A
NM_024865.4(NANOG):c.115C>A (p.Gln39Lys)
MISSENSE
rs2889551 -- p.Lys82Asn

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for NANOG Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for NANOG Gene

Variant ID Type Subtype PubMed ID
dgv57e55 CNV gain 17911159
dgv5e196 CNV duplication 17116639
dgv6e196 CNV duplication 17116639
esv2422393 CNV duplication 17116639
esv2745493 CNV deletion 23290073
esv2759877 CNV gain+loss 17122850
esv3628442 CNV gain 21293372
esv3892131 CNV gain 25118596
nsv1159953 CNV duplication 26073780
nsv428270 CNV gain 18775914
nsv596 CNV insertion 18451855
nsv7227 OTHER inversion 18451855
nsv973012 CNV duplication 23825009

Variation tolerance for NANOG Gene

Residual Variation Intolerance Score: 73.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.56; 44.48% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NANOG Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NANOG
Leiden Open Variation Database (LOVD)
NANOG

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NANOG Gene

Disorders for NANOG Gene

MalaCards: The human disease database

(31) MalaCards diseases for NANOG Gene - From: COP and GCD

Disorder Aliases PubMed IDs
teratocarcinoma
  • mixed embryonal carcinoma and teratoma
germ cell and embryonal cancer
  • germ cell and embryonal neoplasm
embryonal carcinoma
  • primary extragonadal embryonal carcinoma
serous cystadenocarcinoma
  • serous adenocarcinoma
ovarian serous cystadenocarcinoma
  • serous cystadenoma
- elite association - COSMIC cancer census association via MalaCards
Search NANOG in MalaCards View complete list of genes associated with diseases

Additional Disease Information for NANOG

genes like me logo Genes that share disorders with NANOG: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NANOG Gene

Publications for NANOG Gene

  1. Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. (PMID: 15983365) Hyslop L … Lako M (Stem cells (Dayton, Ohio) 2005) 3 4 22
  2. Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. (PMID: 14990856) Clark AT … Reijo Pera RA (Stem cells (Dayton, Ohio) 2004) 3 4 22
  3. Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human. (PMID: 15108323) Hart AH … Robb L (Developmental dynamics : an official publication of the American Association of Anatomists 2004) 3 4 22
  4. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. (PMID: 12787504) Mitsui K … Yamanaka S (Cell 2003) 2 3 4
  5. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. (PMID: 12787505) Chambers I … Smith A (Cell 2003) 2 3 4

Products for NANOG Gene

  • Addgene plasmids for NANOG

Sources for NANOG Gene