This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located i... See more...

Aliases for NAA60 Gene

Aliases for NAA60 Gene

  • N(Alpha)-Acetyltransferase 60, NatF Catalytic Subunit 2 3 5
  • N-Acetyltransferase 15 (GCN5-Related, Putative) 2 3
  • Histone Acetyltransferase Type B Protein 4 3 4
  • N-Alpha-Acetyltransferase 60 3 4
  • N-Alpha-Acetyltransferase F 3 4
  • HNaa60 3 4
  • NAT15 3 4
  • HAT4 3 4
  • NatF 3 4
  • Epididymis Secretory Sperm Binding Protein 3
  • N-Acetyltransferase 15 4
  • EC 2.3.1.259 4
  • EC 2.3.1.48 4

External Ids for NAA60 Gene

Previous HGNC Symbols for NAA60 Gene

  • NAT15

Previous GeneCards Identifiers for NAA60 Gene

  • GC16P003495

Summaries for NAA60 Gene

Entrez Gene Summary for NAA60 Gene

  • This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele. [provided by RefSeq, Nov 2015]

GeneCards Summary for NAA60 Gene

NAA60 (N(Alpha)-Acetyltransferase 60, NatF Catalytic Subunit) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring acyl groups other than amino-acyl groups and peptide alpha-N-acetyltransferase activity.

UniProtKB/Swiss-Prot Summary for NAA60 Gene

  • N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol (PubMed:25732826). Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met (PubMed:21750686, PubMed:25732826, PubMed:27550639, PubMed:27320834). Required for normal chromosomal segregation during anaphase (PubMed:21750686). May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences (PubMed:21981917).

Additional gene information for NAA60 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for NAA60 Gene

Genomics for NAA60 Gene

GeneHancer (GH) Regulatory Elements for NAA60 Gene

Promoters and enhancers for NAA60 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J003455 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas 761 +15.1 15103 6.2 ZNF785 SP1 ZBTB40 CTCF SIX5 LCORL ZBTB6 POLR2A USF2 HCFC1 NAA60 ENSG00000263212 lnc-CLUAP1-6 CREBBP ZNF597 SRRM2 ENSG00000262482 KCTD5 THOC6 AMDHD2
GH16J003442 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 750.6 -0.1 -148 3 SP1 ZBTB40 SREBF1 ZBTB6 POLR2A NFYC ETV6 L3MBTL2 MAX MNT ZNF597 NAA60 piR-56497-056 ZNF200 CREBBP AMDHD2 ENSG00000261938 MEFV TIGD7 ZNF75A
GH16J003400 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 11.9 -42.4 -42448 3.2 ZNF24 SP1 ZBTB40 SIX5 ZBTB6 FOXA1 MLX RBPJ POLR2A NR2F1 ZNF174 ZSCAN32 ZNF597 CREBBP ZNF263 ZNF75A SRRM2 ENSG00000261938 TIGD7 AMDHD2
GH16J003491 Enhancer 0.6 Ensembl 13.3 +47.8 47842 1.4 RAD21 NFIB RXRA REST CTBP1 SAP130 POLR2A NFIC ZFHX2 TFAP4 NAA60 C16orf90 MIR6126 ENSG00000263212
GH16J003290 Promoter 0.7 Ensembl 11.1 -153.2 -153248 0.8 TRIM24 SOX6 MTA3 TRIM28 CBX1 ZEB2 ZBTB33 piR-32653 piR-57460-232 ZNF263 MEFV OR2C1 ZNF174 ZSCAN32 ZNF200 ZNF597 NAA60
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around NAA60 on UCSC Golden Path with GeneCards custom track

Genomic Locations for NAA60 Gene

Genomic Locations for NAA60 Gene
chr16:3,443,649-3,486,963
(GRCh38/hg38)
Size:
43,315 bases
Orientation:
Plus strand
chr16:3,493,611-3,536,963
(GRCh37/hg19)
Size:
43,353 bases
Orientation:
Plus strand

Genomic View for NAA60 Gene

Genes around NAA60 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NAA60 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NAA60 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NAA60 Gene

Proteins for NAA60 Gene

  • Protein details for NAA60 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H7X0-NAA60_HUMAN
    Recommended name:
    N-alpha-acetyltransferase 60
    Protein Accession:
    Q9H7X0
    Secondary Accessions:
    • B3KRQ0
    • B4DLZ0
    • B4DPZ8
    • B4DYC4
    • D3DUC2
    • E7EQ65
    • Q6IA31
    • Q6UX26

    Protein attributes for NAA60 Gene

    Size:
    242 amino acids
    Molecular mass:
    27451 Da
    Quaternary structure:
    • Monomer and homodimer; monomer in presence of substrate and homodimer in its absence (PubMed:27320834).

    Three dimensional structures from OCA and Proteopedia for NAA60 Gene

    Alternative splice isoforms for NAA60 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NAA60 Gene

Post-translational modifications for NAA60 Gene

  • Acetylated: autoacetylation is required for optimal acetyltransferase activity.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for NAA60 Gene

Domains & Families for NAA60 Gene

Gene Families for NAA60 Gene

Protein Domains for NAA60 Gene

Suggested Antigen Peptide Sequences for NAA60 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ54847 (B4DLZ0_HUMAN)
  • N-acetyltransferase 15 (NAT15_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H7X0

UniProtKB/Swiss-Prot:

NAA60_HUMAN :
  • Belongs to the acetyltransferase family. NAA60 subfamily.
Family:
  • Belongs to the acetyltransferase family. NAA60 subfamily.
genes like me logo Genes that share domains with NAA60: view

Function for NAA60 Gene

Molecular function for NAA60 Gene

UniProtKB/Swiss-Prot Function:
N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol (PubMed:25732826). Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met (PubMed:21750686, PubMed:25732826, PubMed:27550639, PubMed:27320834). Required for normal chromosomal segregation during anaphase (PubMed:21750686). May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences (PubMed:21981917).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=acetyl-CoA + N-terminal L-methionyl-[transmembrane protein] = CoA + H(+) + N-terminal N(alpha)-acetyl-L-methionyl-[transmembrane protein]; Xref=Rhea:RHEA:50604, Rhea:RHEA-COMP:12745, Rhea:RHEA-COMP:12746, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:64731, ChEBI:CHEBI:133414; EC=2.3.1.259; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=acetyl-CoA + L-lysyl-[protein] = CoA + H(+) + N(6)-acetyl-L-lysyl-[protein]; Xref=Rhea:RHEA:45948, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:10731, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:61930; EC=2.3.1.48; Evidence=. ;.
UniProtKB/Swiss-Prot Induction:
Isoform 2: Imprinted (PubMed:21593219). Promoter methylation of the paternal allele may restrict expression to the maternal allele in placenta and leukocytes (PubMed:21593219). Isoform 1: Biallelically expressed (PubMed:21593219).

Enzyme Numbers (IUBMB) for NAA60 Gene

Phenotypes From GWAS Catalog for NAA60 Gene

Gene Ontology (GO) - Molecular Function for NAA60 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004402 histone acetyltransferase activity IEA --
GO:0004596 peptide alpha-N-acetyltransferase activity IBA,IDA 21750686
GO:0010485 H4 histone acetyltransferase activity IDA 21981917
GO:0016740 transferase activity IEA --
GO:0016746 transferase activity, transferring acyl groups IEA --
genes like me logo Genes that share ontologies with NAA60: view
genes like me logo Genes that share phenotypes with NAA60: view

Animal Model Products

  • Taconic Biosciences Mouse Models for NAA60

CRISPR Products

miRNA for NAA60 Gene

miRTarBase miRNAs that target NAA60

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NAA60

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NAA60 Gene

Localization for NAA60 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NAA60 Gene

Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably forms an intramembrane hairpin-like structure in the membrane. {ECO:0000305 PubMed:25732826}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NAA60 gene
Compartment Confidence
golgi apparatus 5
cytoskeleton 3
cytosol 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Actin filaments (1)
  • Cytosol (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NAA60 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IDA 21981917
GO:0005794 Golgi apparatus IEA --
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with NAA60: view

Pathways & Interactions for NAA60 Gene

PathCards logo

SuperPathways for NAA60 Gene

No Data Available

Interacting Proteins for NAA60 Gene

Gene Ontology (GO) - Biological Process for NAA60 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006325 chromatin organization IEA --
GO:0006334 nucleosome assembly IDA 21981917
GO:0006474 N-terminal protein amino acid acetylation IDA 25732826
GO:0007059 chromosome segregation ISS --
GO:0008283 cell proliferation IMP 21981917
genes like me logo Genes that share ontologies with NAA60: view

No data available for Pathways by source and SIGNOR curated interactions for NAA60 Gene

Drugs & Compounds for NAA60 Gene

(1) Drugs for NAA60 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Coenzyme A Investigational Nutra 0

(1) Additional Compounds for NAA60 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
acetyl-coa
  • AcCoA
  • Acetyl coenzyme A
  • S-Acetyl-CoA
  • S-Acetyl-coenzyme A
  • Ac-CoA
72-89-9
genes like me logo Genes that share compounds with NAA60: view

Transcripts for NAA60 Gene

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for NAA60

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NAA60 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b
SP1: - - - - -
SP2: - - - - - - -
SP3:
SP4:
SP5: -

Relevant External Links for NAA60 Gene

GeneLoc Exon Structure for
NAA60
ECgene alternative splicing isoforms for
NAA60

Expression for NAA60 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NAA60 Gene

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NAA60 Gene



NURSA nuclear receptor signaling pathways regulating expression of NAA60 Gene:

NAA60

SOURCE GeneReport for Unigene cluster for NAA60 Gene:

Hs.513296

Evidence on tissue expression from TISSUES for NAA60 Gene

  • Kidney(4.3)
  • Nervous system(4.3)
genes like me logo Genes that share expression patterns with NAA60: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for NAA60 Gene

Orthologs for NAA60 Gene

This gene was present in the common ancestor of animals.

Orthologs for NAA60 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia -- 33
  • 99 (a)
OneToMany
dog
(Canis familiaris)
Mammalia -- 33
  • 97 (a)
OneToMany
NAA60 32
  • 91.87 (n)
mouse
(Mus musculus)
Mammalia Naa60 17 33 32
  • 93.25 (n)
rat
(Rattus norvegicus)
Mammalia Naa60 32
  • 92.84 (n)
oppossum
(Monodelphis domestica)
Mammalia -- 33
  • 92 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 33
  • 91 (a)
OneToMany
cow
(Bos Taurus)
Mammalia NAA60 33 32
  • 90.36 (n)
OneToMany
chicken
(Gallus gallus)
Aves NAA60 32
  • 83.88 (n)
-- 33
  • 81 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 33
  • 89 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia naa60 32
  • 75.21 (n)
zebrafish
(Danio rerio)
Actinopterygii nat15 33 32
  • 80.03 (n)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005192 32
  • 57.75 (n)
fruit fly
(Drosophila melanogaster)
Insecta Naa60 33 32
  • 55.95 (n)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea F30F8.10 33 32
  • 46.67 (n)
OneToMany
Species where no ortholog for NAA60 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NAA60 Gene

ENSEMBL:
Gene Tree for NAA60 (if available)
TreeFam:
Gene Tree for NAA60 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for NAA60: view image

Paralogs for NAA60 Gene

No data available for Paralogs for NAA60 Gene

Variants for NAA60 Gene

Sequence variations from dbSNP and Humsavar for NAA60 Gene

SNP ID Clin Chr 16 pos Variation AA Info Type
rs1000001155 -- 3,474,158(+) A/G intron_variant
rs1000007986 -- 3,448,237(+) G/T genic_upstream_transcript_variant, intron_variant
rs1000055627 -- 3,443,745(+) A/C 5_prime_UTR_variant, genic_upstream_transcript_variant
rs1000111807 -- 3,444,239(+) G/T genic_upstream_transcript_variant, intron_variant
rs1000181126 -- 3,472,965(+) C/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for NAA60 Gene

Variant ID Type Subtype PubMed ID
dgv324e201 CNV deletion 23290073
dgv325e201 CNV deletion 23290073
esv2665923 CNV deletion 23128226
esv2750408 CNV deletion 23290073
esv2750409 CNV deletion 23290073
esv29492 CNV gain 19812545
esv3553026 CNV deletion 23714750
esv3637667 CNV gain 21293372
esv3637668 CNV loss 21293372
nsv1051957 CNV loss 25217958
nsv1070738 CNV deletion 25765185
nsv1148032 CNV deletion 26484159
nsv1715 CNV deletion 18451855
nsv512420 CNV loss 21212237
nsv952908 CNV deletion 24416366

Variation tolerance for NAA60 Gene

Residual Variation Intolerance Score: 53.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.15; 39.09% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NAA60 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NAA60

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NAA60 Gene

Disorders for NAA60 Gene

Additional Disease Information for NAA60

No disorders were found for NAA60 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NAA60 Gene

Publications for NAA60 Gene

  1. An organellar nα-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity. (PMID: 25732826) Aksnes H … Arnesen T (Cell reports 2015) 2 3 4 56
  2. NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation. (PMID: 21750686) Van Damme P … Arnesen T (PLoS genetics 2011) 2 3 4 56
  3. Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase. (PMID: 27550639) Chen JY … Yun CH (Scientific reports 2016) 3 4 56
  4. Crystal Structure of the Golgi-Associated Human Nα-Acetyltransferase 60 Reveals the Molecular Determinants for Substrate-Specific Acetylation. (PMID: 27320834) Støve SI … Arnesen T (Structure (London, England : 1993) 2016) 3 4 56
  5. Methylation screening of reciprocal genome-wide UPDs identifies novel human-specific imprinted genes. (PMID: 21593219) Nakabayashi K … Monk D (Human molecular genetics 2011) 3 4 56

Products for NAA60 Gene

Sources for NAA60 Gene