Aliases for MYLK4 Gene

Aliases for MYLK4 Gene

  • Myosin Light Chain Kinase Family Member 4 2 3 4 5
  • SgK085 2 3 4
  • Sugen Kinase 85 3 4
  • CaMLCK Like 2 3
  • EC 4 51
  • Myosin Light Chain Kinase Family, Member 4 2
  • EC 2.7.11 51
  • SGK085 4
  • MYLK4 5

External Ids for MYLK4 Gene

Previous GeneCards Identifiers for MYLK4 Gene

  • GC06M002613

Summaries for MYLK4 Gene

GeneCards Summary for MYLK4 Gene

MYLK4 (Myosin Light Chain Kinase Family Member 4) is a Protein Coding gene. Diseases associated with MYLK4 include Deafness, Autosomal Recessive 14 and Cardiomyopathy, Familial Restrictive, 2. Among its related pathways are Focal Adhesion and Apelin signaling pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MYLK3.

Tocris Summary for MYLK4 Gene

  • Myosin Light Chain Kinases (MLCKs) are a group of protein serine/threonine kinases that are currently divided into two subtypes. MLCK1 is found in smooth muscle and phosphorylates myosin II regulatory light chains at Ser19. MLCK2 is located in the striated muscle.

Additional gene information for MYLK4 Gene

No data available for Entrez Gene Summary , CIViC Summary , UniProtKB/Swiss-Prot Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MYLK4 Gene

Genomics for MYLK4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for MYLK4 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MYLK4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MYLK4

Top Transcription factor binding sites by QIAGEN in the MYLK4 gene promoter:
  • aMEF-2
  • AP-1
  • ATF
  • c-Fos
  • c-Jun
  • FosB
  • Fra-1
  • HNF-4alpha1
  • HNF-4alpha2
  • MEF-2A

Genomic Locations for MYLK4 Gene

Genomic Locations for MYLK4 Gene
106,748 bases
Minus strand
87,338 bases
Minus strand

Genomic View for MYLK4 Gene

Genes around MYLK4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MYLK4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MYLK4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MYLK4 Gene

Proteins for MYLK4 Gene

  • Protein details for MYLK4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Myosin light chain kinase family member 4
    Protein Accession:
    Secondary Accessions:
    • A2RUC0
    • Q5TAW2

    Protein attributes for MYLK4 Gene

    388 amino acids
    Molecular mass:
    44508 Da
    Quaternary structure:
    No Data Available
    • [Isoform 2]: Sequence incomplete.

    Three dimensional structures from OCA and Proteopedia for MYLK4 Gene

    Alternative splice isoforms for MYLK4 Gene


neXtProt entry for MYLK4 Gene

Selected DME Specific Peptides for MYLK4 Gene


Post-translational modifications for MYLK4 Gene

No Post-translational modifications

Other Protein References for MYLK4 Gene

Domains & Families for MYLK4 Gene

Gene Families for MYLK4 Gene

Protein Domains for MYLK4 Gene

Suggested Antigen Peptide Sequences for MYLK4 Gene

GenScript: Design optimal peptide antigens:
  • Sugen kinase 85 (MYLK4_HUMAN)

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
genes like me logo Genes that share domains with MYLK4: view

Function for MYLK4 Gene

Molecular function for MYLK4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=;.

Enzyme Numbers (IUBMB) for MYLK4 Gene

Phenotypes From GWAS Catalog for MYLK4 Gene

Gene Ontology (GO) - Molecular Function for MYLK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity IEA --
GO:0004687 myosin light chain kinase activity IBA 21873635
GO:0005515 protein binding IPI 22939624
genes like me logo Genes that share ontologies with MYLK4: view
genes like me logo Genes that share phenotypes with MYLK4: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MYLK4

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MYLK4 Gene

Localization for MYLK4 Gene

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MYLK4 gene
Compartment Confidence
cytosol 3
nucleus 2
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (2)
  • Nucleoli (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MYLK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with MYLK4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for MYLK4 Gene

Pathways & Interactions for MYLK4 Gene

genes like me logo Genes that share pathways with MYLK4: view

Gene Ontology (GO) - Biological Process for MYLK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA --
GO:0016310 phosphorylation IEA --
genes like me logo Genes that share ontologies with MYLK4: view

No data available for SIGNOR curated interactions for MYLK4 Gene

Drugs & Compounds for MYLK4 Gene

(4) Drugs for MYLK4 Gene - From: DrugBank, HMDB, and Tocris

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
ATP Investigational Nutra Agonist, Activator, Partial agonist, Antagonist, Full agonist, Gating inhibitor, Pore Blocker, Potentiation 0
ML 9 hydrochloride Pharma Myosin light chain kinase inhibitor 0
MLCK inhibitor peptide 18 Pharma Selective inhibitor of myosin light chain kinase 0

(1) Additional Compounds for MYLK4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Agonist, Full agonist, Partial agonist, Gating inhibitor, Antagonist 58-64-0

(2) Tocris Compounds for MYLK4 Gene

Compound Action Cas Number
ML 9 hydrochloride Myosin light chain kinase inhibitor 105637-50-1
MLCK inhibitor peptide 18 Selective inhibitor of myosin light chain kinase 224579-74-2
genes like me logo Genes that share compounds with MYLK4: view

Transcripts for MYLK4 Gene

mRNA/cDNA for MYLK4 Gene

8 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MYLK4

Alternative Splicing Database (ASD) splice patterns (SP) for MYLK4 Gene

No ASD Table

Relevant External Links for MYLK4 Gene

GeneLoc Exon Structure for

Expression for MYLK4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MYLK4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MYLK4 Gene

This gene is overexpressed in Muscle - Skeletal (x13.5).

Protein differential expression in normal tissues from HIPED for MYLK4 Gene

This gene is overexpressed in Heart (68.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for MYLK4 Gene

Protein tissue co-expression partners for MYLK4 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MYLK4

SOURCE GeneReport for Unigene cluster for MYLK4 Gene:


Evidence on tissue expression from TISSUES for MYLK4 Gene

  • Spleen(4)
  • Muscle(2.7)
genes like me logo Genes that share expression patterns with MYLK4: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MYLK4 Gene

Orthologs for MYLK4 Gene

This gene was present in the common ancestor of animals.

Orthologs for MYLK4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia MYLK4 30 31
  • 99.4 (n)
(Canis familiaris)
Mammalia MYLK4 30 31
  • 88.54 (n)
(Ornithorhynchus anatinus)
Mammalia MYLK4 31
  • 85 (a)
(Rattus norvegicus)
Mammalia Mylk4 30
  • 84.89 (n)
(Bos Taurus)
Mammalia MYLK4 30 31
  • 84.48 (n)
(Mus musculus)
Mammalia Mylk4 30 17 31
  • 84.08 (n)
(Monodelphis domestica)
Mammalia MYLK4 31
  • 46 (a)
(Gallus gallus)
Aves MYLK4 30 31
  • 77.26 (n)
(Anolis carolinensis)
Reptilia MYLK4 31
  • 51 (a)
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia LOC100497991 30
  • 70.78 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.2372 30
(Danio rerio)
Actinopterygii LOC566845 30
  • 70.92 (n)
mylk4b 31
  • 32 (a)
mylk4a 31
  • 30 (a)
Fruit Fly
(Drosophila melanogaster)
Insecta sqa 31
  • 16 (a)
(Caenorhabditis elegans)
Secernentea ZC373.4 31
  • 12 (a)
Species where no ortholog for MYLK4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for MYLK4 Gene

Gene Tree for MYLK4 (if available)
Gene Tree for MYLK4 (if available)
Evolutionary constrained regions (ECRs) for MYLK4: view image

Paralogs for MYLK4 Gene

Variants for MYLK4 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MYLK4 Gene

SNP ID Clinical significance and condition Chr 06 pos Variation AA Info Type
719968 Likely Benign: not provided 2,766,396(-) C/G SYNONYMOUS_VARIANT,INTRON_VARIANT
732958 Likely Benign: not provided 2,766,156(-) G/T SYNONYMOUS_VARIANT,INTRON_VARIANT
770919 Benign: not provided 2,768,731(-) A/G MISSENSE_VARIANT,INTRON_VARIANT
rs762093504 A breast infiltrating ductal carcinoma sample p.Ala78Ser
rs796052168 Likely Benign: Long QT syndrome 2,685,390(-) C/T MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for MYLK4 Gene

Structural Variations from Database of Genomic Variants (DGV) for MYLK4 Gene

Variant ID Type Subtype PubMed ID
esv1778065 CNV insertion 17803354
esv2434989 CNV insertion 19546169
esv2731465 CNV deletion 23290073
esv2731466 CNV deletion 23290073
esv274995 CNV loss 21479260
esv3570726 CNV loss 25503493
esv3570727 CNV loss 25503493
esv3570728 CNV loss 25503493
esv3570729 CNV loss 25503493
esv3607900 CNV gain 21293372
esv3607912 CNV gain 21293372
nsv1131934 CNV deletion 24896259
nsv474757 CNV novel sequence insertion 20440878
nsv521426 CNV loss 19592680
nsv830574 CNV gain 17160897

Variation tolerance for MYLK4 Gene

Residual Variation Intolerance Score: 50.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 9.17; 87.88% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MYLK4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MYLK4 Gene

Disorders for MYLK4 Gene

MalaCards: The human disease database

(4) MalaCards diseases for MYLK4 Gene - From: DISEASES

- elite association - COSMIC cancer census association via MalaCards
Search MYLK4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for MYLK4

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with MYLK4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MYLK4 Gene

Publications for MYLK4 Gene

  1. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41
  2. The DNA sequence and analysis of human chromosome 6. (PMID: 14574404) Mungall AJ … Beck S (Nature 2003) 3 4
  3. The protein kinase complement of the human genome. (PMID: 12471243) Manning G … Sudarsanam S (Science (New York, N.Y.) 2002) 3 4
  4. Architecture of the human interactome defines protein communities and disease networks. (PMID: 28514442) Huttlin EL … Harper JW (Nature 2017) 3
  5. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin EL … Gygi SP (Cell 2015) 3

Products for MYLK4 Gene

Sources for MYLK4 Gene