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Aliases for MSLN Gene

Aliases for MSLN Gene

  • Mesothelin 2 3 3 5
  • Pre-Pro-Megakaryocyte-Potentiating Factor 3 4
  • CAK1 Antigen 3 4
  • MPF 3 4
  • Soluble MPF Mesothelin Related Protein 3
  • Megakaryocyte Potentiating Factor 3
  • SMRP 3

External Ids for MSLN Gene

Previous GeneCards Identifiers for MSLN Gene

  • GC16P000829
  • GC16P000750
  • GC16P000751
  • GC16P000811
  • GC16P000732
  • GC16P000810
  • GC16P000697
  • GC16P000702
  • GC16P000724
  • GC16P000774
  • GC16P000791
  • GC16P000839
  • GC16P000869
  • GC16P000899
  • GC16P000916
  • GC16P000937
  • GC16P000958
  • GC16P000986

Summaries for MSLN Gene

Entrez Gene Summary for MSLN Gene

  • This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

GeneCards Summary for MSLN Gene

MSLN (Mesothelin) is a Protein Coding gene. Diseases associated with MSLN include Malignant Pleural Mesothelioma and Mesothelioma, Malignant. Among its related pathways are Metabolism of proteins and Post-translational modification- synthesis of GPI-anchored proteins. An important paralog of this gene is MSLNL.

UniProtKB/Swiss-Prot for MSLN Gene

  • Membrane-anchored forms may play a role in cellular adhesion.

  • Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Gene Wiki entry for MSLN Gene

Additional gene information for MSLN Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MSLN Gene

Genomics for MSLN Gene

GeneHancer (GH) Regulatory Elements for MSLN Gene

Promoters and enhancers for MSLN Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J000759 Promoter/Enhancer 1.6 EPDnew FANTOM5 Ensembl ENCODE 688.5 +0.4 387 3.7 KLF1 MXI1 SREBF2 ZFP64 MAX ZNF266 KLF17 HIC1 ZFHX2 GLIS2 MSLN GC16P001018 GC16P001019 ENSG00000261505 ENSG00000260051 RHOT2 ENSG00000260954 TELO2 AXIN1 WASH4P
GH16J000787 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 9.5 +27.9 27888 2.8 PKNOX1 ARID4B SIN3A ZNF2 YY1 ZNF207 ZNF143 FOS SP3 MXD4 CHTF18 GC16M000970 RPUSD1 WDR90 CRAMP1 ENSG00000261207 WDR24 ENSG00000260051 MSLN MSLNL
GH16J000754 Enhancer 0.8 Ensembl ENCODE 17.5 -5.8 -5831 0.6 MEIS2 KLF1 PKNOX1 L3MBTL2 SOX6 MITF ZNF316 ZNF766 FOXK2 PBX2 MSLN CIAO3
GH16J000806 Enhancer 1.2 Ensembl ENCODE 5.6 +46.9 46869 2.4 HDGF PKNOX1 SIN3A FEZF1 ZNF2 YY1 POLR2B ZNF766 E2F8 ZNF207 CHTF18 ENSG00000261207 MSLN PRR25 GC16P001024
GH16J000757 Enhancer 0.5 Ensembl 9.9 -3.1 -3131 0.8 PKNOX1 GABPB1 CEBPB CBFA2T3 MSLN WASH4P CIAO3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MSLN on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the MSLN gene promoter:
  • p53
  • Max1
  • c-Myc

Genomic Locations for MSLN Gene

Genomic Locations for MSLN Gene
8,134 bases
Plus strand
8,104 bases
Plus strand

Genomic View for MSLN Gene

Genes around MSLN on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MSLN Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MSLN Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MSLN Gene

Proteins for MSLN Gene

  • Protein details for MSLN Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • D3DU65
    • Q14859
    • Q4VQD5
    • Q96GR6
    • Q96KJ5
    • Q9BR17
    • Q9BTR2
    • Q9UCB2
    • Q9UK57

    Protein attributes for MSLN Gene

    630 amino acids
    Molecular mass:
    68986 Da
    Quaternary structure:
    • Interacts with MUC16.

    Three dimensional structures from OCA and Proteopedia for MSLN Gene

    Alternative splice isoforms for MSLN Gene


neXtProt entry for MSLN Gene

Post-translational modifications for MSLN Gene

  • Both MPF and the cleaved form of mesothelin are N-glycosylated.
  • Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Glycosylation at Asn523, isoforms=3, 2, 4496, isoforms=3, 2, 4388, and Asn57
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for MSLN Gene

Domains & Families for MSLN Gene

Gene Families for MSLN Gene

Human Protein Atlas (HPA):
  • Cancer-related genes
  • Disease related genes
  • Plasma proteins
  • Predicted intracellular proteins
  • Predicted membrane proteins
  • Predicted secreted proteins

Protein Domains for MSLN Gene


Suggested Antigen Peptide Sequences for MSLN Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the mesothelin family.
  • Belongs to the mesothelin family.
genes like me logo Genes that share domains with MSLN: view

Function for MSLN Gene

Molecular function for MSLN Gene

UniProtKB/Swiss-Prot Function:
Membrane-anchored forms may play a role in cellular adhesion.
UniProtKB/Swiss-Prot Function:
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
GENATLAS Biochemistry:
Mesothelin,megakaryocyte potentiating factor. Expressed by mesothelial,detectable in sera from patients with ovarian carcinoma. Putatively involved in cell adhesion

Phenotypes From GWAS Catalog for MSLN Gene

Gene Ontology (GO) - Molecular Function for MSLN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 14676194
genes like me logo Genes that share ontologies with MSLN: view
genes like me logo Genes that share phenotypes with MSLN: view

Animal Models for MSLN Gene

MGI Knock Outs for MSLN:

Animal Model Products

miRNA for MSLN Gene

miRTarBase miRNAs that target MSLN

Clone Products

  • Addgene plasmids for MSLN

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MSLN Gene

Localization for MSLN Gene

Subcellular locations from UniProtKB/Swiss-Prot for MSLN Gene

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.
Megakaryocyte-potentiating factor: Secreted.
Isoform 3: Secreted.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MSLN gene
Compartment Confidence
plasma membrane 5
extracellular 5
endoplasmic reticulum 5
golgi apparatus 5
cytoskeleton 1
nucleus 1
lysosome 1
endosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MSLN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005615 extracellular space IEA --
GO:0005788 endoplasmic reticulum lumen TAS --
GO:0005794 Golgi apparatus IEA --
GO:0005886 plasma membrane TAS --
genes like me logo Genes that share ontologies with MSLN: view

Pathways & Interactions for MSLN Gene

genes like me logo Genes that share pathways with MSLN: view

Pathways by source for MSLN Gene

Gene Ontology (GO) - Biological Process for MSLN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006501 C-terminal protein lipidation TAS --
GO:0007155 cell adhesion NAS 8552591
GO:0007160 cell-matrix adhesion IBA --
GO:0031016 pancreas development IEA --
GO:0043687 post-translational protein modification TAS --
genes like me logo Genes that share ontologies with MSLN: view

No data available for SIGNOR curated interactions for MSLN Gene

Drugs & Compounds for MSLN Gene

(4) Drugs for MSLN Gene - From: DrugBank, DGIdb, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Amatuximab Investigational Pharma Target 0
SS1(DSFV)-PE38 Pharma 0

(4) Additional Compounds for MSLN Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with MSLN: view

Transcripts for MSLN Gene

mRNA/cDNA for MSLN Gene

Unigene Clusters for MSLN Gene

Representative Sequences:

Clone Products

  • Addgene plasmids for MSLN

Alternative Splicing Database (ASD) splice patterns (SP) for MSLN Gene

No ASD Table

Relevant External Links for MSLN Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for MSLN Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MSLN Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MSLN Gene

This gene is overexpressed in Adipose - Visceral (Omentum) (x16.4), Lung (x12.1), and Fallopian Tube (x5.2).

Protein differential expression in normal tissues from HIPED for MSLN Gene

This gene is overexpressed in Amniocyte (20.6), Cervix (15.8), Testis (15.1), and Ovary (14.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for MSLN Gene

NURSA nuclear receptor signaling pathways regulating expression of MSLN Gene:


SOURCE GeneReport for Unigene cluster for MSLN Gene:


mRNA Expression by UniProt/SwissProt for MSLN Gene:

Tissue specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

Evidence on tissue expression from TISSUES for MSLN Gene

  • Lung(4.6)
  • Pancreas(4.4)
  • Spleen(4.2)
genes like me logo Genes that share expression patterns with MSLN: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for MSLN Gene

Orthologs for MSLN Gene

This gene was present in the common ancestor of chordates.

Orthologs for MSLN Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia MSLN 34 33
  • 97.26 (n)
(Canis familiaris)
Mammalia MSLN 34 33
  • 76.78 (n)
(Bos Taurus)
Mammalia MSLN 34 33
  • 74.46 (n)
(Rattus norvegicus)
Mammalia Msln 33
  • 71.23 (n)
(Mus musculus)
Mammalia Msln 16 34 33
  • 70.96 (n)
(Monodelphis domestica)
Mammalia MSLN 34
  • 21 (a)
(Gallus gallus)
Aves MSLN 34 33
  • 48.6 (n)
(Anolis carolinensis)
Reptilia MSLN 34
  • 37 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC101733400 33
  • 40.69 (n)
(Danio rerio)
Actinopterygii MSLN (2 of 2) 34
  • 22 (a)
MSLN (1 of 2) 34
  • 6 (a)
Species where no ortholog for MSLN was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MSLN Gene

Gene Tree for MSLN (if available)
Gene Tree for MSLN (if available)
Evolutionary constrained regions (ECRs) for MSLN: view image

Paralogs for MSLN Gene

Paralogs for MSLN Gene

genes like me logo Genes that share paralogs with MSLN: view

Variants for MSLN Gene

Sequence variations from dbSNP and Humsavar for MSLN Gene

SNP ID Clin Chr 16 pos Variation AA Info Type
rs1000192562 -- 762,918(+) G/A intron_variant
rs1000310920 -- 766,625(+) C/T intron_variant
rs1000355230 -- 759,561(+) T/C upstream_transcript_variant
rs1000606789 -- 763,180(+) G/T intron_variant
rs1001052642 -- 769,086(+) C/T downstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for MSLN Gene

Variant ID Type Subtype PubMed ID
dgv4825n54 CNV loss 21841781
dgv4827n54 CNV loss 21841781
dgv4828n54 CNV gain 21841781
dgv4829n54 CNV loss 21841781
dgv4830n54 CNV gain+loss 21841781
dgv4831n54 CNV gain 21841781
dgv772e212 CNV loss 25503493
esv2422427 CNV duplication 17116639
esv27245 CNV gain+loss 19812545
esv3637582 CNV gain 21293372
nsv1070732 CNV deletion 25765185
nsv1143797 CNV deletion 24896259
nsv1160325 CNV duplication 26073780
nsv1160326 CNV duplication 26073780
nsv1702 CNV insertion 18451855
nsv457311 CNV loss 19166990
nsv457312 CNV loss 19166990
nsv471063 CNV loss 18288195
nsv517488 CNV loss 19592680
nsv526903 CNV loss 19592680
nsv571023 CNV loss 21841781
nsv571025 CNV gain 21841781
nsv571030 CNV gain 21841781
nsv571032 CNV loss 21841781
nsv571033 CNV gain 21841781
nsv571034 CNV gain 21841781
nsv9321 CNV gain+loss 18304495
nsv952898 CNV deletion 24416366

Variation tolerance for MSLN Gene

Residual Variation Intolerance Score: 99.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.39; 71.07% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MSLN Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MSLN Gene

Disorders for MSLN Gene

MalaCards: The human disease database

(18) MalaCards diseases for MSLN Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
malignant pleural mesothelioma
  • malignant mesothelioma of pleura
mesothelioma, malignant
  • mesom
malignant biphasic mesothelioma
  • mesothelioma, biphasic, malignant
pancreatic cancer
  • pancreatic carcinoma
ovarian cancer
  • ovarian cancer, somatic
- elite association - COSMIC cancer census association via MalaCards
Search MSLN in MalaCards View complete list of genes associated with diseases


  • Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. {ECO:0000269 PubMed:15897581}.

Additional Disease Information for MSLN

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with MSLN: view

No data available for Genatlas for MSLN Gene

Publications for MSLN Gene

  1. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. (PMID: 8552591) Chang K … Pastan I (Proceedings of the National Academy of Sciences of the United States of America 1996) 2 3 4 22 58
  2. Megakaryocyte potentiation factor cleaved from mesothelin precursor is a useful tumor marker in the serum of patients with mesothelioma. (PMID: 16857795) Onda M … Pastan I (Clinical cancer research : an official journal of the American Association for Cancer Research 2006) 3 4 22 58
  3. Humoral immune response to mesothelin in mesothelioma and ovarian cancer patients. (PMID: 15897581) Ho M … Pastan I (Clinical cancer research : an official journal of the American Association for Cancer Research 2005) 3 4 22 58
  4. Binding of ovarian cancer antigen CA125/MUC16 to mesothelin mediates cell adhesion. (PMID: 14676194) Rump A … Miyajima A (The Journal of biological chemistry 2004) 3 4 22 58
  5. Characterization of human mesothelin transcripts in ovarian and pancreatic cancer. (PMID: 15140265) Muminova ZE … Shaw DR (BMC cancer 2004) 3 4 22 58

Products for MSLN Gene

Sources for MSLN Gene

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