This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese... See more...

Aliases for MPST Gene

Aliases for MPST Gene

  • Mercaptopyruvate Sulfurtransferase 2 3 5
  • TRNA Thiouridin Modification Protein 1 2 3
  • 3-Mercaptopyruvate Sulfurtransferase 3 4
  • Human Liver Rhodanese 2 3
  • TST2 3 4
  • MST 3 4
  • Testicular Tissue Protein Li 200 3
  • EC 2.8.1.2 4
  • TUM1 3

External Ids for MPST Gene

Previous GeneCards Identifiers for MPST Gene

  • GC22P034030
  • GC22P035658
  • GC22P035740
  • GC22P037415
  • GC22P020380

Summaries for MPST Gene

Entrez Gene Summary for MPST Gene

  • This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for MPST Gene

MPST (Mercaptopyruvate Sulfurtransferase) is a Protein Coding gene. Diseases associated with MPST include Mercaptolactate-Cysteine Disulfiduria and Cyanide Poisoning. Among its related pathways are Amino Acid metabolism and Metabolism. Gene Ontology (GO) annotations related to this gene include thiosulfate sulfurtransferase activity and 3-mercaptopyruvate sulfurtransferase activity. An important paralog of this gene is TST.

UniProtKB/Swiss-Prot Summary for MPST Gene

  • Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.

Gene Wiki entry for MPST Gene

Additional gene information for MPST Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MPST Gene

Genomics for MPST Gene

GeneHancer (GH) Regulatory Elements for MPST Gene

Promoters and enhancers for MPST Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MPST on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MPST

Top Transcription factor binding sites by QIAGEN in the MPST gene promoter:
  • AML1a
  • En-1
  • HNF-4alpha1
  • HNF-4alpha2
  • POU2F1
  • POU2F1a
  • RFX1
  • USF-1
  • USF1
  • ZID

Genomic Locations for MPST Gene

Genomic Locations for MPST Gene
chr22:37,019,635-37,029,822
(GRCh38/hg38)
Size:
10,188 bases
Orientation:
Plus strand
chr22:37,415,676-37,425,863
(GRCh37/hg19)
Size:
10,188 bases
Orientation:
Plus strand

Genomic View for MPST Gene

Genes around MPST on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MPST Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MPST Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MPST Gene

Proteins for MPST Gene

  • Protein details for MPST Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P25325-THTM_HUMAN
    Recommended name:
    3-mercaptopyruvate sulfurtransferase
    Protein Accession:
    P25325
    Secondary Accessions:
    • A8MZ34
    • B3KP52
    • J3KPV7
    • O75750
    • Q6FHN9

    Protein attributes for MPST Gene

    Size:
    297 amino acids
    Molecular mass:
    33178 Da
    Quaternary structure:
    • Monomer (active form). Homodimer; disulfide-linked (inactive form).
    Miscellaneous:
    • Thioredoxin (Trx) or dihydrolipoic acid (DHLA) are required to release hydrogen sulfide from the persulfide intermediate.
    SequenceCaution:
    • Sequence=AAH16737.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=CAG30409.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MPST Gene

    Alternative splice isoforms for MPST Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MPST Gene

Post-translational modifications for MPST Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for MPST Gene

Domains & Families for MPST Gene

Gene Families for MPST Gene

IUPHAR :
Human Protein Atlas (HPA):
  • Disease related genes
  • Enzymes
  • Plasma proteins
  • Potential drug targets
  • Predicted intracellular proteins

Protein Domains for MPST Gene

Blocks:
  • Rhodanese-like
  • Thiosulfate sulfurtransferase
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MPST Gene

GenScript: Design optimal peptide antigens:
  • Truncated mercaptopyruvate sulfurtransferase variant (Q2VEU0_HUMAN)
  • Mercaptopyruvate sulfurtransferase variant (Q2VEU1_HUMAN)
  • Mercaptopyruvate sulfurtransferase, isoform CRA_a (Q6FHN9_HUMAN)
  • 3-mercaptopyruvate sulfurtransferase (THTM_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P25325

UniProtKB/Swiss-Prot:

THTM_HUMAN :
  • Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).
Domain:
  • Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).
genes like me logo Genes that share domains with MPST: view

Function for MPST Gene

Molecular function for MPST Gene

UniProtKB/Swiss-Prot Function:
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=3-mercaptopyruvate + [thioredoxin]-dithiol = [thioredoxin]-disulfide + H(+) + hydrogen sulfide + pyruvate; Xref=Rhea:RHEA:21740, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:29919, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:57678; EC=2.8.1.2; Evidence=. ;.
UniProtKB/Swiss-Prot EnzymeRegulation:
By oxidative stress, and thioredoxin. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate which inhibits the MPST enzyme activity. Reduced thioredoxin cleaves an intersubunit disulfide bond to turn on the redox switch and reactivate the enzyme.
GENATLAS Biochemistry:
mercaptopyruvate sulfurtransferase

Enzyme Numbers (IUBMB) for MPST Gene

Phenotypes From GWAS Catalog for MPST Gene

Gene Ontology (GO) - Molecular Function for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004792 thiosulfate sulfurtransferase activity IBA,IEA 21873635
GO:0016740 transferase activity IEA --
GO:0016784 3-mercaptopyruvate sulfurtransferase activity TAS --
GO:0042802 identical protein binding IEA --
genes like me logo Genes that share ontologies with MPST: view
genes like me logo Genes that share phenotypes with MPST: view

Animal Models for MPST Gene

MGI Knock Outs for MPST:
  • Mpst Mpst<tm1.1Nori>

Animal Model Products

  • Taconic Biosciences Mouse Models for MPST

CRISPR Products

miRNA for MPST Gene

miRTarBase miRNAs that target MPST

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MPST Gene

Localization for MPST Gene

Subcellular locations from UniProtKB/Swiss-Prot for MPST Gene

Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MPST gene
Compartment Confidence
mitochondrion 5
cytosol 4
plasma membrane 2
extracellular 2
nucleus 2
cytoskeleton 1
peroxisome 1
endoplasmic reticulum 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Mitochondria (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IBA 21873635
GO:0005759 mitochondrial matrix TAS --
GO:0005829 cytosol IEA --
GO:0030054 cell junction IEA --
genes like me logo Genes that share ontologies with MPST: view

Pathways & Interactions for MPST Gene

genes like me logo Genes that share pathways with MPST: view

Pathways by source for MPST Gene

Gene Ontology (GO) - Biological Process for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000098 sulfur amino acid catabolic process TAS --
GO:0001822 kidney development IEA --
GO:0001889 liver development IEA --
GO:0009440 cyanate catabolic process TAS 9070219
GO:0009636 response to toxic substance TAS 9070219
genes like me logo Genes that share ontologies with MPST: view

No data available for SIGNOR curated interactions for MPST Gene

Drugs & Compounds for MPST Gene

(3) Drugs for MPST Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Pyruvic acid Approved, Investigational Nutra 0

(10) Additional Compounds for MPST Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
cyanide
  • Carbon nitride ion
  • Chuck norrisium
  • Cyanide ion
  • Cyanide(1-) ion
  • Cyano
57-12-5
thiocyanate
  • [S-C#N](-)
  • N#C-S(-)
  • Rhodanid
  • Rhodanide
  • SCN(-)
302-04-5
thiosulfate
  • [SO2(OH)(SH)]
  • H2S2O3
  • Thiosulfuric acid
  • Hydroxidodioxidosulfidosulfuric acid
  • Hydroxidodioxidosulphidosulphate
14383-50-7
3-Mercaptolactate-cysteine disulfide
  • 3-((2-Carboxy-2-hydroxyethyl)dithio)-L-alanine
  • beta-Mercaptolactate cysteine disulfide
  • Hcetc
  • S-(2-Hydroxy-2-carboxyethylthio)cysteine
18841-42-4
3-Mercaptopyruvic acid
  • 3-mercapto-2-Oxopropanoic acid
  • 3-Mercaptopyruvate
  • Mercaptopyruvate
  • 3-mercapto-2-Oxopropanoate
  • Mercaptopyruvic acid
2464-23-5
genes like me logo Genes that share compounds with MPST: view

Transcripts for MPST Gene

mRNA/cDNA for MPST Gene

6 REFSEQ mRNAs :
19 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for MPST Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d · 8e
SP1: -
SP2: - - - -
SP3: - - -
SP4:
SP5: -
SP6:
SP7: - - -

Relevant External Links for MPST Gene

GeneLoc Exon Structure for
MPST

Expression for MPST Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MPST Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MPST Gene

This gene is overexpressed in Liver (x7.1).

Protein differential expression in normal tissues from HIPED for MPST Gene

This gene is overexpressed in Liver, secretome (16.3) and Liver (9.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MPST Gene



Protein tissue co-expression partners for MPST Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MPST

SOURCE GeneReport for Unigene cluster for MPST Gene:

Hs.248267

Evidence on tissue expression from TISSUES for MPST Gene

  • Kidney(4.5)
  • Liver(4.5)
  • Nervous system(4.5)
  • Muscle(4.3)
  • Pancreas(4.3)
  • Bone marrow(4.2)
  • Blood(2.1)
genes like me logo Genes that share expression patterns with MPST: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MPST Gene

Orthologs for MPST Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for MPST Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MPST 31 30
  • 98.84 (n)
OneToOne
dog
(Canis familiaris)
Mammalia MPST 31 30
  • 89.9 (n)
OneToOne
cow
(Bos Taurus)
Mammalia MPST 31 30
  • 86.46 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Mpst 17 31 30
  • 83.67 (n)
rat
(Rattus norvegicus)
Mammalia Mpst 30
  • 82.43 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia MPST 31
  • 80 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MPST 31
  • 79 (a)
OneToOne
chicken
(Gallus gallus)
Aves MPST 31 30
  • 70.61 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MPST 31
  • 75 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mpst 30
  • 63.96 (n)
Str.1400 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.3678 30
zebrafish
(Danio rerio)
Actinopterygii zgc:162544 30
  • 58.68 (n)
MPST 31
  • 56 (a)
OneToMany
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2809 30
worm
(Caenorhabditis elegans)
Secernentea mpst-6 31
  • 26 (a)
ManyToMany
mpst-5 31
  • 26 (a)
ManyToMany
mpst-4 31
  • 23 (a)
ManyToMany
mpst-2 31
  • 23 (a)
ManyToMany
mpst-3 31
  • 22 (a)
ManyToMany
mpst-1 31
  • 21 (a)
ManyToMany
mpst-7 31
  • 18 (a)
ManyToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TUM1 33 31 30
  • 46.04 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AAL096W 30
  • 45.48 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C09966g 30
  • 44.44 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons MST1 30
  • 48.23 (n)
rice
(Oryza sativa)
Liliopsida Os12g0608600 30
  • 48.11 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU06017 30
  • 49.72 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes tum1 30
  • 45.31 (n)
Species where no ortholog for MPST was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MPST Gene

ENSEMBL:
Gene Tree for MPST (if available)
TreeFam:
Gene Tree for MPST (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MPST: view image

Paralogs for MPST Gene

Paralogs for MPST Gene

(1) SIMAP similar genes for MPST Gene using alignment to 6 proteins:

  • THTM_HUMAN
  • B1AH49_HUMAN
  • J3KPV7_HUMAN
  • Q2VEU0_HUMAN
  • Q2VEU1_HUMAN
  • Q6FHN9_HUMAN
genes like me logo Genes that share paralogs with MPST: view

Variants for MPST Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MPST Gene

SNP ID Clinical significance and condition Chr 22 pos Variation AA Info Type
684529 Not Provided: Mercaptolactate-cysteine disulfiduria 37,024,469(+) A/G MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for MPST Gene

Structural Variations from Database of Genomic Variants (DGV) for MPST Gene

Variant ID Type Subtype PubMed ID
esv2661268 CNV deletion 23128226
esv34015 CNV loss 18971310
esv3558139 CNV deletion 23714750
esv3647699 CNV loss 21293372
nsv1057409 CNV gain 25217958
nsv3622 CNV insertion 18451855
nsv459882 CNV gain 19166990
nsv588984 CNV gain 21841781
nsv829203 CNV loss 20364138
nsv834187 CNV loss 17160897

Variation tolerance for MPST Gene

Residual Variation Intolerance Score: 45.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.80; 33.89% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MPST Gene

Human Gene Mutation Database (HGMD)
MPST
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MPST

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MPST Gene

Disorders for MPST Gene

MalaCards: The human disease database

(5) MalaCards diseases for MPST Gene - From: DISEASES and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search MPST in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

THTM_HUMAN
  • Note=Aberrant MPST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine (PubMed:4973015, PubMed:4690911 and PubMed:6945862). Some cases have associated mental retardation (PubMed:4973015 and PubMed:6945862).

Additional Disease Information for MPST

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with MPST: view

No data available for Genatlas for MPST Gene

Publications for MPST Gene

  1. Cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes. (PMID: 1953758) Pallini R … Cacace MG (Biochemical and biophysical research communications 1991) 2 3 4 54
  2. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson SL … O'Brien SJ (PloS one 2010) 3 41 54
  3. Evidence for a functional genetic polymorphism of the human mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification enzyme. (PMID: 16545926) Billaut-Laden I … Broly F (Toxicology letters 2006) 3 23 54
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 54

Products for MPST Gene

Sources for MPST Gene