This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] See more...

Aliases for MKI67 Gene

Aliases for MKI67 Gene

  • Marker Of Proliferation Ki-67 2 3 5
  • Antigen Identified By Monoclonal Antibody Ki-67 2 3 4
  • Protein Phosphatase 1, Regulatory Subunit 105 2 3
  • Proliferation Marker Protein Ki-67 3 4
  • Antigen Ki67 3 4
  • Proliferation-Related Ki-67 Antigen 3
  • Antigen KI-67 4
  • PPP1R105 3
  • MIB-1 3
  • MIB- 3
  • KIA 3

External Ids for MKI67 Gene

Previous GeneCards Identifiers for MKI67 Gene

  • GC10M128707
  • GC10M128936
  • GC10M129824
  • GC10M129369
  • GC10M129784
  • GC10M123469
  • GC10M129894

Summaries for MKI67 Gene

Entrez Gene Summary for MKI67 Gene

  • This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

CIViC Summary for MKI67 Gene

GeneCards Summary for MKI67 Gene

MKI67 (Marker Of Proliferation Ki-67) is a Protein Coding gene. Diseases associated with MKI67 include Cervical Intraepithelial Neoplasia and Bowen's Disease. Among its related pathways are DNA Damage and Neuroscience. Gene Ontology (GO) annotations related to this gene include protein C-terminus binding. An important paralog of this gene is CDCA2.

UniProtKB/Swiss-Prot Summary for MKI67 Gene

  • Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Gene Wiki entry for MKI67 Gene

Additional gene information for MKI67 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MKI67 Gene

Genomics for MKI67 Gene

GeneHancer (GH) Regulatory Elements for MKI67 Gene

Promoters and enhancers for MKI67 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH10J128124 Promoter/Enhancer 1.6 Ensembl ENCODE CraniofacialAtlas 500.7 +0.7 722 3.8 SIN3A NRF1 POLR2G SP1 ZFX ZNF10 CLOCK POLR2A FOS ZBTB26 MKI67 lnc-CLRN3-3 lnc-PTPRE-3
GH10J128149 Promoter/Enhancer 1.2 ENCODE CraniofacialAtlas 12.8 -23.5 -23493 1.2 CTCF ZIC2 GABPA ZBTB26 REST KLF7 ZNF740 MYC RCOR1 CTBP1 MKI67 LINC01163 lnc-PTPRE-3
GH10J128095 Enhancer 0.9 FANTOM5 Ensembl ENCODE 12.7 +30.4 30422 2 POLR2A BCL6 EGR1 JUND GLIS1 MAX EGR2 PRDM10 GLIS2 PRDM1 MKI67 lnc-CLRN3-3 lnc-PTPRE-3
GH10J128147 Enhancer 0.8 Ensembl ENCODE 12.8 -21.0 -20978 1.6 RELA FOS NBN EZH2 ELF1 JUND IKZF1 PAX5 TBX21 NFIC MKI67 LINC01163 lnc-PTPRE-3
GH10J128085 Enhancer 0.7 Ensembl ENCODE 12.5 +40.3 40322 3.4 ZNF384 POLR2A NFE2 CTCF SMARCA4 TFAP4 SP5 PAX5 EMSY PTPRE MKI67 CLRN3 lnc-PTPRE-3 lnc-CLRN3-3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MKI67 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MKI67

Top Transcription factor binding sites by QIAGEN in the MKI67 gene promoter:
  • Cart-1
  • CHOP-10
  • CUTL1
  • Evi-1
  • Gfi-1
  • TBP

Genomic Locations for MKI67 Gene

Genomic Locations for MKI67 Gene
chr10:128,096,659-128,126,423
(GRCh38/hg38)
Size:
29,765 bases
Orientation:
Minus strand
chr10:129,894,923-129,924,649
(GRCh37/hg19)
Size:
29,727 bases
Orientation:
Minus strand

Genomic View for MKI67 Gene

Genes around MKI67 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MKI67 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MKI67 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MKI67 Gene

Proteins for MKI67 Gene

  • Protein details for MKI67 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P46013-KI67_HUMAN
    Recommended name:
    Proliferation marker protein Ki-67
    Protein Accession:
    P46013
    Secondary Accessions:
    • Q5VWH2

    Protein attributes for MKI67 Gene

    Size:
    3256 amino acids
    Molecular mass:
    358694 Da
    Quaternary structure:
    • Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (PubMed:19131338, PubMed:23178126).

    Three dimensional structures from OCA and Proteopedia for MKI67 Gene

    Alternative splice isoforms for MKI67 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MKI67 Gene

Post-translational modifications for MKI67 Gene

  • Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
  • Ubiquitination at Lys659, Lys666, Lys745, Lys1043, Lys1549, Lys1592, Lys2138, and Lys2289
  • Modification sites at PhosphoSitePlus

Other Protein References for MKI67 Gene

Antibody Products

  • Abcam antibodies for MKI67

No data available for DME Specific Peptides for MKI67 Gene

Domains & Families for MKI67 Gene

Gene Families for MKI67 Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for MKI67 Gene

Blocks:
  • Forkhead-associated (FHA)
  • K167R
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MKI67 Gene

GenScript: Design optimal peptide antigens:
  • Antigen KI-67 (KI67_HUMAN)
  • KI67 Antigen (Q70L73_HUMAN)
  • KI67 Antigen (Q70L74_HUMAN)
genes like me logo Genes that share domains with MKI67: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for MKI67 Gene

Function for MKI67 Gene

Molecular function for MKI67 Gene

UniProtKB/Swiss-Prot Function:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
GENATLAS Biochemistry:
antigen identified by monoclonal antibody Ki-67 (non histone protein 395/345kDa)

Phenotypes From GWAS Catalog for MKI67 Gene

Gene Ontology (GO) - Molecular Function for MKI67 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003723 RNA binding HDA 22658674
GO:0005515 protein binding IEA,IPI 10878014
GO:0005524 ATP binding IEA --
GO:0008022 protein C-terminus binding IPI 17577209
genes like me logo Genes that share ontologies with MKI67: view
genes like me logo Genes that share phenotypes with MKI67: view

Animal Model Products

CRISPR Products

miRNA for MKI67 Gene

miRTarBase miRNAs that target MKI67

Clone Products

  • Addgene plasmids for MKI67

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MKI67 Gene

Localization for MKI67 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MKI67 Gene

Chromosome. Nucleus. Nucleus, nucleolus. Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). {ECO:0000269 PubMed:15896774, ECO:0000269 PubMed:22002106, ECO:0000269 PubMed:27362226}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MKI67 gene
Compartment Confidence
nucleus 5
mitochondrion 3
plasma membrane 2
extracellular 2
cytoskeleton 2
cytosol 2
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoli (4)
  • Nucleoplasm (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MKI67 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000793 colocalizes_with condensed chromosome IDA 27362226
GO:0005634 nucleus IEA,IDA 22378020
GO:0005654 nucleoplasm IDA --
GO:0005694 chromosome IEA --
GO:0005730 nucleolus IEA,IDA 17577209
genes like me logo Genes that share ontologies with MKI67: view

Pathways & Interactions for MKI67 Gene

genes like me logo Genes that share pathways with MKI67: view

Pathways by source for MKI67 Gene

1 BioSystems pathway for MKI67 Gene
2 Cell Signaling Technology pathways for MKI67 Gene

Gene Ontology (GO) - Biological Process for MKI67 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007049 cell cycle IEA --
GO:0007088 regulation of mitotic nuclear division IDA 27362226
GO:0008283 cell proliferation TAS 17577209
GO:0051983 regulation of chromosome segregation IDA 27362226
GO:1902275 regulation of chromatin organization ISS --
genes like me logo Genes that share ontologies with MKI67: view

No data available for SIGNOR curated interactions for MKI67 Gene

Drugs & Compounds for MKI67 Gene

(47) Drugs for MKI67 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(34) Additional Compounds for MKI67 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with MKI67: view

Transcripts for MKI67 Gene

mRNA/cDNA for MKI67 Gene

2 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

  • Addgene plasmids for MKI67

Alternative Splicing Database (ASD) splice patterns (SP) for MKI67 Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15a · 15b ^ 16a · 16b · 16c ^ 17
SP1: - - - - - - -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7: -
SP8: -

Relevant External Links for MKI67 Gene

GeneLoc Exon Structure for
MKI67

Expression for MKI67 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MKI67 Gene

mRNA differential expression in normal tissues according to GTEx for MKI67 Gene

This gene is overexpressed in Esophagus - Mucosa (x4.3).

Protein differential expression in normal tissues from HIPED for MKI67 Gene

This gene is overexpressed in Placenta (20.3), Kidney (13.4), Fetal testis (8.5), Liver (7.5), and Esophagus (6.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MKI67 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MKI67

SOURCE GeneReport for Unigene cluster for MKI67 Gene:

Hs.80976

Evidence on tissue expression from TISSUES for MKI67 Gene

  • Intestine(3.4)
  • Blood(2.8)
  • Liver(2.8)
  • Lung(2.2)
  • Lymph node(2.2)
  • Bone marrow(2)
genes like me logo Genes that share expression patterns with MKI67: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MKI67 Gene

Orthologs for MKI67 Gene

This gene was present in the common ancestor of chordates.

Orthologs for MKI67 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MKI67 31 30
  • 98.9 (n)
OneToOne
dog
(Canis familiaris)
Mammalia MKI67 31 30
  • 68.91 (n)
OneToOne
cow
(Bos Taurus)
Mammalia MKI67 31 30
  • 65.49 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mki67 30
  • 64.34 (n)
mouse
(Mus musculus)
Mammalia Mki67 17 31 30
  • 63.29 (n)
oppossum
(Monodelphis domestica)
Mammalia MKI67 31
  • 22 (a)
OneToOne
chicken
(Gallus gallus)
Aves MKI67 31
  • 16 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MKI67 31
  • 24 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.6364 30
zebrafish
(Danio rerio)
Actinopterygii mki67 31
  • 18 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 34 (a)
OneToOne
Species where no ortholog for MKI67 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MKI67 Gene

ENSEMBL:
Gene Tree for MKI67 (if available)
TreeFam:
Gene Tree for MKI67 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MKI67: view image

Paralogs for MKI67 Gene

Paralogs for MKI67 Gene

Pseudogenes.org Pseudogenes for MKI67 Gene

genes like me logo Genes that share paralogs with MKI67: view

Variants for MKI67 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MKI67 Gene

SNP ID Clinical significance and condition Chr 10 pos Variation AA Info Type
rs796052184 Likely Benign: Long QT syndrome 128,104,734(-) A/G MISSENSE_VARIANT
rs10082391 - p.Asp2760Gly
rs10082533 - p.Ser2793Asn
rs1050767 - p.Thr2720Pro
rs1063535 - p.Pro2608Leu

Additional dbSNP identifiers (rs#s) for MKI67 Gene

Structural Variations from Database of Genomic Variants (DGV) for MKI67 Gene

Variant ID Type Subtype PubMed ID
dgv52n21 CNV loss 19592680
dgv989n100 CNV gain 25217958
esv2660735 CNV deletion 23128226
esv2676916 CNV deletion 23128226
esv34105 CNV loss 18971310
esv3624896 CNV loss 21293372
nsv467481 CNV loss 19166990
nsv523484 CNV loss 19592680
nsv552312 CNV loss 21841781
nsv552313 CNV loss 21841781

Variation tolerance for MKI67 Gene

Residual Variation Intolerance Score: 99.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 25.13; 99.69% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MKI67 Gene

Human Gene Mutation Database (HGMD)
MKI67
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MKI67

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MKI67 Gene

Disorders for MKI67 Gene

MalaCards: The human disease database

(61) MalaCards diseases for MKI67 Gene - From: DISEASES, Novoseek, and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search MKI67 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for MKI67

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with MKI67: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MKI67 Gene

Publications for MKI67 Gene

  1. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. (PMID: 27362226) Cuylen S … Gerlich DW (Nature 2016) 3 4 54
  2. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. (PMID: 24867636) Booth DG … Vagnarelli P (eLife 2014) 3 4 54
  3. Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study. (PMID: 20800603) Flachsbart F … Nebel A (Mutation research 2010) 3 41 54
  4. Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot". (PMID: 20628624) Johnatty SE … Australian Cancer Study (Ovarian Cancer) (PLoS genetics 2010) 3 41 54
  5. Steroid receptors, HER2/neu and Ki-67, in endometrioid type of endometrial carcinoma: Correlation with conventional histomorphological features of prognosis. (PMID: 19446313) Chakravarty D … Dhaliwal L (Acta histochemica 2010) 3 23 54

Products for MKI67 Gene

  • Addgene plasmids for MKI67

Sources for MKI67 Gene