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MFNG Gene(Protein Coding)

MFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase

GCID:
GC22M037469
GIFtS:
49
Genes Participants
UDN Logo

Aliases for MFNG Gene

Aliases for MFNG Gene

  • MFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase 2 3 5
  • EC 2.4.1.222 4 56
  • O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase 4
  • Manic Fringe (Drosophila) Homolog 2
  • Manic Fringe Homolog (Drosophila) 2

External Ids for MFNG Gene

Previous GeneCards Identifiers for MFNG Gene

  • GC22M034479
  • GC22M036108
  • GC22M036189
  • GC22M037865
  • GC22M020831

Summaries for MFNG Gene

Entrez Gene Summary for MFNG Gene

  • This gene is a member of the fringe gene family which also includes radical and lunatic fringe genes. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. [provided by RefSeq, Oct 2009]

GeneCards Summary for MFNG Gene

MFNG (MFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase) is a Protein Coding gene. Among its related pathways are Development NOTCH1-mediated pathway for NF-KB activity modulation and PI3K-Akt signaling pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring glycosyl groups and O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity. An important paralog of this gene is LFNG.

UniProtKB/Swiss-Prot for MFNG Gene

  • Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules (PubMed:10935626). Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity).

Gene Wiki entry for MFNG Gene

Additional gene information for MFNG Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MFNG Gene

Genomics for MFNG Gene

Products:

  1. Regulatory Element

GeneHancer (GH) Regulatory Elements for MFNG Gene

Promoters and enhancers for MFNG Gene
GeneHancer (GH) Identifier GH Type GH
Score		 GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites		 Gene Targets
GH22I037482 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE dbSUPER 550.8 +1.4 1353 4.7 HDGF PKNOX1 SIN3A ZNF121 ZNF207 ZNF143 ATF7 RUNX3 SP3 SP5 GC22P037483 MFNG EIF3L ENSG00000100101 H1F0 EIF3D CARD10
GH22I037487 Enhancer 0.4 ENCODE dbSUPER 550.8 -1.3 -1258 0.2 MFNG GC22P037518
GH22I037430 Enhancer 1.3 Ensembl ENCODE dbSUPER 11.1 +54.3 54256 2.5 SIN3A FEZF1 BRCA1 GLIS2 ZNF143 FOS RUNX3 KLF13 SP3 JUNB ELFN2 ANKRD54 MFNG PLA2G6 IL2RB TEX33 GC22P037457
GH22I037441 Enhancer 0.7 dbSUPER 11.5 +43.7 43691 1.7 USF1 CEBPG ETS1 ZNF507 ZNF143 THAP11 HCFC1 HLF NFYC USF2 MFNG CYTH4 IL2RB GC22P037457 ELFN2
GH22I037227 Promoter/Enhancer 2.8 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 2.8 +249.7 249733 18.7 HDGF PKNOX1 SMAD1 ARNT POLR2B E2F8 ZNF207 ZNF143 FOS REST RAC2 CYTH4 GGA1 CSF2RB SSTR3 C1QTNF6 NCF4 SH3BP1 ENSG00000226233 MFNG
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around MFNG on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the MFNG gene promoter:

Genomic Locations for MFNG Gene

Genomic Locations for MFNG Gene
chr22:37,469,063-37,486,440
(GRCh38/hg38)
Size:
17,378 bases
Orientation:
Minus strand
chr22:37,865,101-37,882,478
(GRCh37/hg19)

Genomic View for MFNG Gene

Genes around MFNG on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MFNG Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MFNG Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for MFNG Gene

Proteins for MFNG Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for MFNG Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O00587-MFNG_HUMAN
    Recommended name:
    Beta-1,3-N-acetylglucosaminyltransferase manic fringe
    Protein Accession:
    O00587
    Secondary Accessions:
    • B4DLT6
    • O43730
    • Q504S9

    Protein attributes for MFNG Gene

    Size:
    321 amino acids
    Molecular mass:
    36202 Da
    Cofactor:
    Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for MFNG Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MFNG Gene

Selected DME Specific Peptides for MFNG Gene

O00587:
  • DDDNYVN
  • CHVDDDNY
  • ALSCKMA
  • QVTLSYG
  • LYPDTPWCP
  • HCLLYPDT
  • RLPDDCT
  • FIAVKTT
  • FWFATGGAGFC

Post-translational modifications for MFNG Gene

  • Glycosylation at posLast=109109 and isoforms=2185
  • Modification sites at PhosphoSitePlus

Other Protein References for MFNG Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for MFNG Gene

Gene Families for MFNG Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins
  • Predicted secreted proteins

Protein Domains for MFNG Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for MFNG Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O00587

UniProtKB/Swiss-Prot:

MFNG_HUMAN :
  • Belongs to the glycosyltransferase 31 family.
Family:
  • Belongs to the glycosyltransferase 31 family.
genes like me logo Genes that share domains with MFNG: view

Function for MFNG Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line

Molecular function for MFNG Gene

GENATLAS Biochemistry:
manic fringe,putative regulator of developmental processes through regulation of the sensitivity of NOTCH receptor genes or their specific ligands,essential mediator of somite segmentaion and patterning
UniProtKB/Swiss-Prot CatalyticActivity:
Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.
UniProtKB/Swiss-Prot Function:
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules (PubMed:10935626). Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity).

Enzyme Numbers (IUBMB) for MFNG Gene

Phenotypes From GWAS Catalog for MFNG Gene

Gene Ontology (GO) - Molecular Function for MFNG Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016740 transferase activity IEA --
GO:0016757 transferase activity, transferring glycosyl groups IEA --
GO:0033829 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with MFNG: view
genes like me logo Genes that share phenotypes with MFNG: view

Animal Models for MFNG Gene

MGI Knock Outs for MFNG:

Animal Model Products

  • Taconic Biosciences Mouse Models for MFNG

miRNA for MFNG Gene

miRTarBase miRNAs that target MFNG

Inhibitory RNA Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MFNG Gene

Localization for MFNG Gene

Subcellular locations from UniProtKB/Swiss-Prot for MFNG Gene

Golgi apparatus membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MFNG gene
Compartment Confidence
extracellular 5
golgi apparatus 4
plasma membrane 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Actin filaments (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MFNG Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005615 extracellular space TAS 9187150
GO:0005794 Golgi apparatus IEA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0030173 integral component of Golgi membrane IEA --
genes like me logo Genes that share ontologies with MFNG: view

Pathways & Interactions for MFNG Gene

genes like me logo Genes that share pathways with MFNG: view

Pathways by source for MFNG Gene

1 BioSystems pathway for MFNG Gene
1 GeneGo (Thomson Reuters) pathway for MFNG Gene
1 R&D Systems pathway for MFNG Gene
1 Qiagen pathway for MFNG Gene

SIGNOR curated interactions for MFNG Gene

Activates:

Gene Ontology (GO) - Biological Process for MFNG Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002315 marginal zone B cell differentiation ISS --
GO:0007275 multicellular organism development IEA --
GO:0007389 pattern specification process IEA,TAS --
GO:0008593 regulation of Notch signaling pathway ISS --
GO:0032092 positive regulation of protein binding IEA --
genes like me logo Genes that share ontologies with MFNG: view

Drugs & Compounds for MFNG Gene

Products:

  1. Drug
No Compound Related Data Available

Transcripts for MFNG Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone

mRNA/cDNA for MFNG Gene

(2) REFSEQ mRNAs :
(8) Additional mRNA sequences :
(246) Selected AceView cDNA sequences:
(10) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for MFNG Gene

MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase:
Representative Sequences:

Inhibitory RNA Products

Alternative Splicing Database (ASD) splice patterns (SP) for MFNG Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c · 4d ^ 5a · 5b ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c
SP1: - - - -
SP2: - -
SP3: -
SP4: - - -
SP5: - - - - -
SP6: - -
SP7: - - -
SP8: - - - - - -
SP9: -
SP10: -

Relevant External Links for MFNG Gene

GeneLoc Exon Structure for
MFNG
ECgene alternative splicing isoforms for
MFNG

Expression for MFNG Gene

Products:

  1. Primer
  2. Gene Expression Assay

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MFNG Gene

mRNA expression in normal human tissues for MFNG Gene
mRNA expression by BioGPS for MFNG GenemRNA expression by GTEx for MFNG Gene

mRNA differential expression in normal tissues according to GTEx for MFNG Gene

This gene is overexpressed in Whole Blood (x9.9) and Spleen (x5.8).

NURSA nuclear receptor signaling pathways regulating expression of MFNG Gene:

MFNG

SOURCE GeneReport for Unigene cluster for MFNG Gene:

Hs.517603
genes like me logo Genes that share expression patterns with MFNG: view

No data available for Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt , Evidence on tissue expression from TISSUES and Phenotype-based relationships between genes and organs from Gene ORGANizer for MFNG Gene

Orthologs for MFNG Gene

This gene was present in the common ancestor of animals.

Orthologs for MFNG Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia MFNG 33 34
  • 98.96 (n)
cow
(Bos Taurus)
 Mammalia MFNG 33 34
  • 87.08 (n)
dog
(Canis familiaris)
 Mammalia MFNG 33 34
  • 86.8 (n)
mouse
(Mus musculus)
 Mammalia Mfng 33 16 34
  • 83.65 (n)
rat
(Rattus norvegicus)
 Mammalia Mfng 33
  • 82.81 (n)
platypus
(Ornithorhynchus anatinus)
 Mammalia MFNG 34
  • 68 (a)
 OneToOne
oppossum
(Monodelphis domestica)
 Mammalia MFNG 34
  • 67 (a)
 OneToOne
chicken
(Gallus gallus)
 Aves MFNG 33 34
  • 71.02 (n)
lizard
(Anolis carolinensis)
 Reptilia -- 34
  • 72 (a)
 OneToMany
-- 34
  • 60 (a)
 OneToMany
zebrafish
(Danio rerio)
 Actinopterygii mfng 33 34
  • 62.05 (n)
fruit fly
(Drosophila melanogaster)
 Insecta fng 35 34
  • 44 (a)
sea squirt
(Ciona savignyi)
 Ascidiacea -- 34
  • 43 (a)
 ManyToMany
-- 34
  • 42 (a)
 ManyToMany
-- 34
  • 41 (a)
 ManyToMany
-- 34
  • 40 (a)
 ManyToMany
-- 34
  • 40 (a)
 ManyToMany
-- 34
  • 40 (a)
 ManyToMany
-- 34
  • 39 (a)
 ManyToMany
-- 34
  • 39 (a)
 ManyToMany
-- 34
  • 39 (a)
 ManyToMany
-- 34
  • 39 (a)
 ManyToMany
-- 34
  • 39 (a)
 ManyToMany
-- 34
  • 38 (a)
 ManyToMany
-- 34
  • 36 (a)
 ManyToMany
-- 34
  • 35 (a)
 ManyToMany
-- 34
  • 35 (a)
 ManyToMany
-- 34
  • 35 (a)
 ManyToMany
-- 34
  • 35 (a)
 ManyToMany
Species where no ortholog for MFNG was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MFNG Gene

ENSEMBL:
Gene Tree for MFNG (if available)
TreeFam:
Gene Tree for MFNG (if available)

Paralogs for MFNG Gene

Paralogs for MFNG Gene

(2) SIMAP similar genes for MFNG Gene using alignment to 8 proteins:

genes like me logo Genes that share paralogs with MFNG: view

Variants for MFNG Gene

Products:

  1. SNP Genotyping and Copy Number Assay

Sequence variations from dbSNP and Humsavar for MFNG Gene

SNP ID Clin Chr 22 pos Variation AA Info Type
rs1000180139 -- 37,472,483(-) T/C coding_sequence_variant, missense_variant, non_coding_transcript_variant
rs1000340869 -- 37,469,594(-) A/G 3_prime_UTR_variant, non_coding_transcript_variant
rs1000463680 -- 37,472,777(-) C/T intron_variant
rs1000623917 -- 37,469,298(-) T/G 3_prime_UTR_variant, non_coding_transcript_variant
rs1000630885 -- 37,481,256(-) C/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for MFNG Gene

Variant ID Type Subtype PubMed ID
esv3647709 CNV loss 21293372
nsv1057617 CNV gain 25217958
nsv829203 CNV loss 20364138

Variation tolerance for MFNG Gene

Residual Variation Intolerance Score: 48.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.82; 34.16% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MFNG Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MFNG

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MFNG Gene

Disorders for MFNG Gene

Additional Disease Information for MFNG

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for MFNG Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MFNG Gene

Publications for MFNG Gene

  1. A family of mammalian Fringe genes implicated in boundary determination and the Notch pathway. (PMID: 9187150) Johnston SH … Vogt TF (Development (Cambridge, England) 1997) 2 3 4 58
  2. A genome annotation-driven approach to cloning the human ORFeome. (PMID: 15461802) Collins JE … Dunham I (Genome biology 2004) 3 4 58
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58
  4. Fringe is a glycosyltransferase that modifies Notch. (PMID: 10935626) Moloney DJ … Vogt TF (Nature 2000) 3 4 58
  5. The DNA sequence of human chromosome 22. (PMID: 10591208) Dunham I … O'Brien KP (Nature 1999) 3 4 58

Products for MFNG Gene

Sources for MFNG Gene

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