This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] See more...

Aliases for MED4 Gene

Aliases for MED4 Gene

  • Mediator Complex Subunit 4 2 3 4 5
  • Vitamin D3 Receptor-Interacting Protein Complex 36 KDa Component 3 4
  • Mediator Of RNA Polymerase II Transcription Subunit 4 3 4
  • Activator-Recruited Cofactor 36 KDa Component 3 4
  • DRIP36 3 4
  • VDRIP 3 4
  • ARC36 3 4
  • Mediator Of RNA Polymerase II Transcription, Subunit 4 Homolog (S. Cerevisiae) 2
  • Vitamin D Receptor-Interacting Protein, 36-KD 3
  • Vitamin D Receptor Interacting Protein 2
  • TRAP/SMCC/PC2 Subunit P36 Subunit 4
  • Mediator, 34-KD Subunit, Homolog 3
  • TRAP/SMCC/PC2 Subunit P36 3
  • HSPC126 3
  • TRAP36 3

External Ids for MED4 Gene

Previous HGNC Symbols for MED4 Gene

  • VDRIP

Previous GeneCards Identifiers for MED4 Gene

  • GC13M047550
  • GC13M048627
  • GC13M029442

Summaries for MED4 Gene

Entrez Gene Summary for MED4 Gene

  • This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

GeneCards Summary for MED4 Gene

MED4 (Mediator Complex Subunit 4) is a Protein Coding gene. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Developmental Biology. Gene Ontology (GO) annotations related to this gene include nuclear receptor transcription coactivator activity.

UniProtKB/Swiss-Prot Summary for MED4 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Wiki entry for MED4 Gene

Additional gene information for MED4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MED4 Gene

Genomics for MED4 Gene

GeneHancer (GH) Regulatory Elements for MED4 Gene

Promoters and enhancers for MED4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH13J048093 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 765.9 +0.9 864 3 SP1 ZBTB40 CTCF FOXA1 RBPJ POLR2A ATF1 CREB1 HCFC1 MIXL1 MED4 SUCLA2 PPP1R26P1 MED4-AS1 ITM2B
GH13J048096 Enhancer 0.9 Ensembl ENCODE 750.6 -1.5 -1460 1.6 NR2F6 RAD21 SP1 NCOA3 CEBPG PPARG HNF1A RXRA SOX13 GATAD2A MED4 MED4-AS1 ITM2B SUCLA2 RF00017-1423
GH13J048036 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas 42.5 +56.4 56420 5 GTF3C2 SIN3A ZBTB40 SP1 CTCF FOXA1 RBPJ POLR2A CTBP1 HDAC2 SUCLA2 NUDT15 L13714-106 PPP1R26P1 MED4 SUCLA2-AS1
GH13J048230 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 21.9 -139.4 -139352 7.7 ZNF24 CTCF FOXA1 POLR2A NR2F6 ZNF362 MYC ELF1 RELA HLF ITM2B NONHSAG013474.2 lnc-MED4-3 MED4 LPAR6 RB1 lnc-RB1-1
GH13J047999 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 12.4 +94.2 94177 4.3 MLX POLR2A HCFC1 RAD21 SREBF1 ZNF652 SP1 ZNF280D NKRF ELF3 SUCLA2 SUCLA2-AS1 piR-34822-078 lnc-MED4-2 PPP1R26P1 MED4 MED4-AS1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MED4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the MED4 gene promoter:
  • AP-1
  • c-Fos
  • c-Jun
  • Nkx2-5
  • p300
  • POU3F1
  • PPAR-gamma1
  • PPAR-gamma2
  • SRY
  • STAT5B

Genomic Locations for MED4 Gene

Genomic Locations for MED4 Gene
chr13:48,053,323-48,095,141
(GRCh38/hg38)
Size:
41,819 bases
Orientation:
Minus strand
chr13:48,627,459-48,669,267
(GRCh37/hg19)
Size:
41,809 bases
Orientation:
Minus strand

Genomic View for MED4 Gene

Genes around MED4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED4 Gene

Proteins for MED4 Gene

  • Protein details for MED4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NPJ6-MED4_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 4
    Protein Accession:
    Q9NPJ6
    Secondary Accessions:
    • B4DX67
    • Q53GB4
    • Q53H68
    • Q5T912
    • Q6FHC4
    • Q6IA79
    • Q9BS95
    • Q9NYR5

    Protein attributes for MED4 Gene

    Size:
    270 amino acids
    Molecular mass:
    29745 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

    Alternative splice isoforms for MED4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED4 Gene

Post-translational modifications for MED4 Gene

  • Ubiquitination at Lys55, Lys81, Lys98, Lys112, and Lys150
  • Modification sites at PhosphoSitePlus

Other Protein References for MED4 Gene

No data available for DME Specific Peptides for MED4 Gene

Domains & Families for MED4 Gene

Gene Families for MED4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for MED4 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED4 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ50738, highly similar to Mediator complex subunit 4 (B4DX67_HUMAN)
  • Vitamin D3 receptor-interacting protein complex 36 kDa component (MED4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9NPJ6

UniProtKB/Swiss-Prot:

MED4_HUMAN :
  • Belongs to the Mediator complex subunit 4 family.
Family:
  • Belongs to the Mediator complex subunit 4 family.
genes like me logo Genes that share domains with MED4: view

Function for MED4 Gene

Molecular function for MED4 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Phenotypes From GWAS Catalog for MED4 Gene

Gene Ontology (GO) - Molecular Function for MED4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003712 transcription coregulator activity IDA 10882111
GO:0005515 protein binding IPI 14638676
GO:0030374 nuclear receptor transcription coactivator activity NAS 10235266
GO:0042809 vitamin D receptor binding NAS 10235266
GO:0046966 thyroid hormone receptor binding IDA 10198638
genes like me logo Genes that share ontologies with MED4: view
genes like me logo Genes that share phenotypes with MED4: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED4

Clone Products

  • Addgene plasmids for MED4

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MED4 Gene

Localization for MED4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED4 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED4 gene
Compartment Confidence
nucleus 5
cytosol 2
extracellular 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for MED4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA,IEA 10235267
GO:0005654 nucleoplasm TAS --
GO:0016020 membrane HDA 19946888
GO:0016592 mediator complex IEA,IDA 10882111
GO:0070847 core mediator complex IBA 21873635
genes like me logo Genes that share ontologies with MED4: view

Pathways & Interactions for MED4 Gene

genes like me logo Genes that share pathways with MED4: view

Gene Ontology (GO) - Biological Process for MED4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006357 regulation of transcription by RNA polymerase II IBA,IEA 21873635
GO:0006366 transcription by RNA polymerase II IDA 10882111
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0045893 positive regulation of transcription, DNA-templated IDA 10198638
genes like me logo Genes that share ontologies with MED4: view

No data available for SIGNOR curated interactions for MED4 Gene

Drugs & Compounds for MED4 Gene

(1) Drugs for MED4 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(1) Additional Compounds for MED4 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with MED4: view

Transcripts for MED4 Gene

mRNA/cDNA for MED4 Gene

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED4

Clone Products

  • Addgene plasmids for MED4

Alternative Splicing Database (ASD) splice patterns (SP) for MED4 Gene

No ASD Table

Relevant External Links for MED4 Gene

GeneLoc Exon Structure for
MED4
ECgene alternative splicing isoforms for
MED4

Expression for MED4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MED4 Gene

Protein differential expression in normal tissues from HIPED for MED4 Gene

This gene is overexpressed in Breast (16.9), Liver (13.5), Monocytes (11.2), and Peripheral blood mononuclear cells (6.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MED4 Gene



Protein tissue co-expression partners for MED4 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of MED4 Gene:

MED4

SOURCE GeneReport for Unigene cluster for MED4 Gene:

Hs.181112

Evidence on tissue expression from TISSUES for MED4 Gene

  • Nervous system(4.7)
  • Intestine(4.3)
  • Blood(4.1)
  • Skin(2.1)
genes like me logo Genes that share expression patterns with MED4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED4 Gene

Orthologs for MED4 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED4 33 32
  • 99.88 (n)
OneToOne
cow
(Bos Taurus)
Mammalia MED4 33 32
  • 91.34 (n)
OneToOne
dog
(Canis familiaris)
Mammalia MED4 33 32
  • 91.21 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Med4 32
  • 87.6 (n)
mouse
(Mus musculus)
Mammalia Med4 17 33 32
  • 87.21 (n)
oppossum
(Monodelphis domestica)
Mammalia MED4 33
  • 87 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED4 33
  • 74 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED4 33 32
  • 81.99 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MED4 33
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med4 32
  • 80.06 (n)
Str.17110 32
African clawed frog
(Xenopus laevis)
Amphibia Xl.8738 32
zebrafish
(Danio rerio)
Actinopterygii med4 33 32
  • 72.08 (n)
OneToOne
Dr.18060 32
fruit fly
(Drosophila melanogaster)
Insecta MED4 33 32
  • 53.15 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta MED4_ANOGA 32
  • 51.01 (n)
worm
(Caenorhabditis elegans)
Secernentea mdt-4 33
  • 27 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4706 33
  • 42 (a)
OneToOne
Species where no ortholog for MED4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MED4 Gene

ENSEMBL:
Gene Tree for MED4 (if available)
TreeFam:
Gene Tree for MED4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MED4: view image

Paralogs for MED4 Gene

(6) SIMAP similar genes for MED4 Gene using alignment to 3 proteins:

  • MED4_HUMAN
  • Q5T911_HUMAN
  • U4PSC4_HUMAN
genes like me logo Genes that share paralogs with MED4: view

No data available for Paralogs for MED4 Gene

Variants for MED4 Gene

Sequence variations from dbSNP and Humsavar for MED4 Gene

SNP ID Clin Chr 13 pos Variation AA Info Type
rs1000198369 -- 48,082,217(-) G/A intron_variant
rs1000532691 -- 48,080,592(-) A/G intron_variant
rs1000557701 -- 48,093,142(-) G/A intron_variant
rs1000586458 -- 48,080,320(-) C/T intron_variant
rs1000586691 -- 48,077,402(-) T/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for MED4 Gene

Variant ID Type Subtype PubMed ID
esv2676750 CNV deletion 23128226
esv3632017 CNV loss 21293372
nsv832601 CNV gain 17160897

Variation tolerance for MED4 Gene

Residual Variation Intolerance Score: 52.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.58; 30.49% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MED4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED4

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED4 Gene

Disorders for MED4 Gene

Additional Disease Information for MED4

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for MED4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MED4 Gene

Publications for MED4 Gene

  1. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. (PMID: 11042152) Zhang QH … Chen Z (Genome research 2000) 2 3 4 56
  2. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. (PMID: 10235266) Rachez C … Freedman LP (Nature 1999) 2 3 4 56
  3. MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X … Roeder RG (Molecular cell 2005) 3 4 56
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 56
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 56

Products for MED4 Gene

Sources for MED4 Gene