The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TF... See more...

Aliases for MED27 Gene

Aliases for MED27 Gene

  • Mediator Complex Subunit 27 2 3 4 5
  • Cofactor Required For Sp1 Transcriptional Activation, Subunit 8, 34kDa 2 3
  • Mediator Of RNA Polymerase II Transcription Subunit 27 3 4
  • Transcriptional Coactivator CRSP34 3 4
  • P37 TRAP/SMCC/PC2 Subunit 3 4
  • CRSP Complex Subunit 8 3 4
  • CRSP34 3 4
  • CRSP8 3 4
  • Cofactor Required For Sp1 Transcriptional Activation Subunit 8 4
  • Epididymis Secretory Sperm Binding Protein 3
  • CRAP34 3
  • TRAP37 3
  • MED3 3

External Ids for MED27 Gene

Previous HGNC Symbols for MED27 Gene

  • CRSP8

Previous GeneCards Identifiers for MED27 Gene

  • GC09M133726
  • GC09M134735
  • GC09M104229

Summaries for MED27 Gene

Entrez Gene Summary for MED27 Gene

  • The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

GeneCards Summary for MED27 Gene

MED27 (Mediator Complex Subunit 27) is a Protein Coding gene. Diseases associated with MED27 include Cerebellar Medulloblastoma. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Gene Expression. Gene Ontology (GO) annotations related to this gene include transcription coactivator activity.

UniProtKB/Swiss-Prot Summary for MED27 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Wiki entry for MED27 Gene

Additional gene information for MED27 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MED27 Gene

Genomics for MED27 Gene

GeneHancer (GH) Regulatory Elements for MED27 Gene

Promoters and enhancers for MED27 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH09J132077 Promoter/Enhancer 2.4 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 502.5 +0.7 735 2.7 RXRA ZBTB40 ZNF217 EP300 SIN3A TCF12 POLR2G SIX5 ZFX ZNF639 MED27 lnc-RAPGEF1-3 NONHSAG053659.2
GH09J133029 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 20.9 -950.7 -950714 3.6 ZBTB40 ZNF217 EP300 ZSCAN5C SIN3A ZNF300 TCF12 POLR2G SP1 NCOR1 GTF3C5 LOC105376306 RF00017-8034 TTF1 TSC1 DDX31 GTF3C4 MED22 SETX BRD3OS
GH09J132944 Promoter/Enhancer 2.3 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas 12.4 -864.4 -864414 3.4 EP300 FOXK2 ZBTB40 ZNF217 ZSCAN5C SIN3A TCF12 POLR2G USF1 SP1 TSC1 GFI1B MIR548AW lnc-SPACA9-1 MED22 SETX GTF3C5 TTF1 MED27 AK8
GH09J132668 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 12.4 -590.8 -590814 4.2 HNRNPK ZBTB40 ZNF217 SIN3A NRF1 TCF12 MYC POLR2G USF1 GTF2E2 DDX31 GTF3C4 lnc-DDX31-3 piR-50566 piR-48348-092 MED22 SETX TSC1 TTF1 GTF3C5
GH09J132040 Enhancer 1.1 Ensembl ENCODE dbSUPER 20.7 +37.1 37086 4.4 JUND FOS ATF2 RBM25 FOXA2 ZNF316 NFIC ZBTB17 GATA3 GABPA MED27 ENSG00000176868 POMT1 NONHSAG053659.2 lnc-RAPGEF1-3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MED27 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MED27

Top Transcription factor binding sites by QIAGEN in the MED27 gene promoter:
  • AP-1
  • c-Fos
  • c-Jun
  • Cart-1
  • FosB
  • Fra-1
  • Sox9
  • SREBP-1a
  • SREBP-1b
  • SREBP-1c

Genomic Locations for MED27 Gene

Genomic Locations for MED27 Gene
chr9:131,852,928-132,079,887
(GRCh38/hg38)
Size:
226,960 bases
Orientation:
Minus strand
chr9:134,735,494-134,955,295
(GRCh37/hg19)
Size:
219,802 bases
Orientation:
Minus strand

Genomic View for MED27 Gene

Genes around MED27 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED27 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED27 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED27 Gene

Proteins for MED27 Gene

  • Protein details for MED27 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q6P2C8-MED27_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 27
    Protein Accession:
    Q6P2C8
    Secondary Accessions:
    • O95401
    • Q4F964
    • Q5VTA4
    • Q5VTA5
    • Q9BU57
    • Q9NYR4
    • V9GYV9

    Protein attributes for MED27 Gene

    Size:
    311 amino acids
    Molecular mass:
    35432 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
    SequenceCaution:
    • Sequence=AAH02878.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=AAH02878.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};

    Alternative splice isoforms for MED27 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED27 Gene

Post-translational modifications for MED27 Gene

  • Ubiquitination at Lys50, Lys112, Lys116, Lys134, Lys145, Lys233, and Lys275
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for MED27 Gene

Domains & Families for MED27 Gene

Gene Families for MED27 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for MED27 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED27 Gene

GenScript: Design optimal peptide antigens:
  • Transcriptional coactivator CRSP34 (MED27_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q6P2C8

UniProtKB/Swiss-Prot:

MED27_HUMAN :
  • Belongs to the Mediator complex subunit 27 family.
Family:
  • Belongs to the Mediator complex subunit 27 family.
genes like me logo Genes that share domains with MED27: view

Function for MED27 Gene

Molecular function for MED27 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Phenotypes From GWAS Catalog for MED27 Gene

Gene Ontology (GO) - Molecular Function for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003713 transcription coactivator activity IDA,TAS 9989412
GO:0005515 protein binding IPI 12584197
GO:0061630 ubiquitin protein ligase activity IEA --
genes like me logo Genes that share ontologies with MED27: view
genes like me logo Genes that share phenotypes with MED27: view

Animal Model Products

CRISPR Products

Clone Products

  • Addgene plasmids for MED27

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for MED27 Gene

Localization for MED27 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED27 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED27 gene
Compartment Confidence
nucleus 5
cytosol 4
plasma membrane 1
cytoskeleton 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoli (3)
  • Nucleoplasm (3)
  • Cytosol (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IEA --
GO:0005634 nucleus IEA,IDA 9989412
GO:0005654 nucleoplasm TAS --
GO:0005667 transcription factor complex IDA 9989412
GO:0005730 nucleolus IDA --
genes like me logo Genes that share ontologies with MED27: view

Pathways & Interactions for MED27 Gene

genes like me logo Genes that share pathways with MED27: view

Pathways by source for MED27 Gene

1 GeneGo (Thomson Reuters) pathway for MED27 Gene
  • Transcription Ligand-Dependent Transcription of Retinoid-Target genes

Gene Ontology (GO) - Biological Process for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006357 regulation of transcription by RNA polymerase II IDA 9989412
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0016567 protein ubiquitination IEA --
GO:0019827 stem cell population maintenance IEA --
GO:1903508 positive regulation of nucleic acid-templated transcription IEA --
genes like me logo Genes that share ontologies with MED27: view

No data available for SIGNOR curated interactions for MED27 Gene

Drugs & Compounds for MED27 Gene

No Compound Related Data Available

Transcripts for MED27 Gene

mRNA/cDNA for MED27 Gene

3 REFSEQ mRNAs :
17 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

  • Addgene plasmids for MED27

Alternative Splicing Database (ASD) splice patterns (SP) for MED27 Gene

No ASD Table

Relevant External Links for MED27 Gene

GeneLoc Exon Structure for
MED27

Expression for MED27 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MED27 Gene

Protein differential expression in normal tissues from HIPED for MED27 Gene

This gene is overexpressed in Breast (35.2) and Monocytes (18.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MED27 Gene



Protein tissue co-expression partners for MED27 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MED27

SOURCE GeneReport for Unigene cluster for MED27 Gene:

Hs.374262

Evidence on tissue expression from TISSUES for MED27 Gene

  • Nervous system(3.1)
genes like me logo Genes that share expression patterns with MED27: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED27 Gene

Orthologs for MED27 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED27 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED27 31
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED27 31
  • 99 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MED27 31
  • 97 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED27 31 30
  • 94.53 (n)
OneToOne
cow
(Bos Taurus)
Mammalia MED27 31 30
  • 91.1 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Med27 17 31 30
  • 88.21 (n)
rat
(Rattus norvegicus)
Mammalia Med27 30
  • 88.1 (n)
chicken
(Gallus gallus)
Aves MED27 31 30
  • 84.31 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MED27 31
  • 87 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med27 30
  • 80.49 (n)
Str.899 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.23148 30
zebrafish
(Danio rerio)
Actinopterygii med27 31 30
  • 75.78 (n)
OneToOne
zgc66302 30
fruit fly
(Drosophila melanogaster)
Insecta MED27 31 30
  • 50.74 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta MED27_ANOGA 30
  • 50.4 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 38 (a)
OneToOne
Species where no ortholog for MED27 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MED27 Gene

ENSEMBL:
Gene Tree for MED27 (if available)
TreeFam:
Gene Tree for MED27 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MED27: view image

Paralogs for MED27 Gene

Pseudogenes.org Pseudogenes for MED27 Gene

genes like me logo Genes that share paralogs with MED27: view

No data available for Paralogs for MED27 Gene

Variants for MED27 Gene

Additional dbSNP identifiers (rs#s) for MED27 Gene

Structural Variations from Database of Genomic Variants (DGV) for MED27 Gene

Variant ID Type Subtype PubMed ID
esv2481552 CNV deletion 19546169
esv2619384 CNV insertion 19546169
esv2659649 CNV deletion 23128226
esv2662096 CNV deletion 23128226
esv2677413 CNV deletion 23128226
esv3402046 CNV duplication 20981092
esv3432278 CNV duplication 20981092
esv3444132 CNV duplication 20981092
esv3573403 CNV loss 25503493
esv3573404 CNV loss 25503493
esv3621896 CNV gain 21293372
esv3621898 CNV gain 21293372
esv3621902 CNV gain 21293372
esv3621904 CNV loss 21293372
esv3621906 CNV loss 21293372
esv3621907 CNV loss 21293372
esv3621908 CNV loss 21293372
esv3621909 CNV loss 21293372
nsv1038424 CNV gain 25217958
nsv1051484 CNV loss 25217958
nsv512994 CNV insertion 21212237

Variation tolerance for MED27 Gene

Residual Variation Intolerance Score: 35.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.17; 39.38% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MED27 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED27

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MED27 Gene

Disorders for MED27 Gene

MalaCards: The human disease database

(1) MalaCards diseases for MED27 Gene - From: DISEASES

Disorder Aliases PubMed IDs
cerebellar medulloblastoma
- elite association - COSMIC cancer census association via MalaCards
Search MED27 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for MED27

genes like me logo Genes that share disorders with MED27: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED27 Gene

Publications for MED27 Gene

  1. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. (PMID: 9989412) Ryu S … Tjian R (Nature 1999) 2 3 4 54
  2. MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X … Roeder RG (Molecular cell 2005) 3 4 54
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54
  4. DNA sequence and analysis of human chromosome 9. (PMID: 15164053) Humphray SJ … Dunham I (Nature 2004) 3 4 54
  5. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S … Conaway RC (Molecular cell 2004) 3 4 54

Products for MED27 Gene

Sources for MED27 Gene