This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a ... See more...

Aliases for MED20 Gene

Aliases for MED20 Gene

  • Mediator Complex Subunit 20 2 3 4 5
  • Mediator Of RNA Polymerase II Transcription Subunit 20 3 4
  • TRFP 3 4
  • Trf (TATA Binding Protein-Related Factor)-Proximal Homolog (Drosophila) 2
  • Trf (TATA Binding Protein-Related Factor)-Proximal Homolog 3
  • TRF-Proximal Protein Homolog 4
  • PRO0213 3
  • HTRFP 4
  • SRB2 3

External Ids for MED20 Gene

Previous HGNC Symbols for MED20 Gene

  • TRFP

Previous GeneCards Identifiers for MED20 Gene

  • GC06M041983
  • GC06M041877
  • GC06M041591

Summaries for MED20 Gene

Entrez Gene Summary for MED20 Gene

  • This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

GeneCards Summary for MED20 Gene

MED20 (Mediator Complex Subunit 20) is a Protein Coding gene. Diseases associated with MED20 include Waardenburg Syndrome, Type 3 and Waardenburg Syndrome, Type 1. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Gene Expression. Gene Ontology (GO) annotations related to this gene include DNA-directed 5'-3' RNA polymerase activity.

UniProtKB/Swiss-Prot Summary for MED20 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Additional gene information for MED20 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MED20 Gene

Genomics for MED20 Gene

GeneHancer (GH) Regulatory Elements for MED20 Gene

Promoters and enhancers for MED20 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MED20 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MED20

Top Transcription factor binding sites by QIAGEN in the MED20 gene promoter:
  • AhR
  • AML1a
  • C/EBPbeta
  • E47
  • Hand1
  • p53
  • Sp1

Genomic Locations for MED20 Gene

Genomic Locations for MED20 Gene
chr6:41,905,354-41,921,139
(GRCh38/hg38)
Size:
15,786 bases
Orientation:
Minus strand
chr6:41,873,092-41,888,877
(GRCh37/hg19)
Size:
15,786 bases
Orientation:
Minus strand

Genomic View for MED20 Gene

Genes around MED20 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED20 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED20 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED20 Gene

Proteins for MED20 Gene

  • Protein details for MED20 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H944-MED20_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 20
    Protein Accession:
    Q9H944
    Secondary Accessions:
    • B4DE08
    • O95821
    • Q5T8J4
    • Q9Y429

    Protein attributes for MED20 Gene

    Size:
    212 amino acids
    Molecular mass:
    23222 Da
    Quaternary structure:
    • Interacts with PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

    Alternative splice isoforms for MED20 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED20 Gene

Post-translational modifications for MED20 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Abcam antibodies for MED20
  • Boster Bio Antibodies for MED20

No data available for DME Specific Peptides for MED20 Gene

Domains & Families for MED20 Gene

Gene Families for MED20 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for MED20 Gene

Blocks:
  • TATA-binding related factor
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED20 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ60159, highly similar to TRF-proximal protein homolog (B3KUJ9_HUMAN)
  • cDNA FLJ53711, highly similar to TRF-proximal protein homolog (B4DE08_HUMAN)
  • TRF-proximal protein homolog (MED20_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H944

UniProtKB/Swiss-Prot:

MED20_HUMAN :
  • Belongs to the Mediator complex subunit 20 family.
Family:
  • Belongs to the Mediator complex subunit 20 family.
genes like me logo Genes that share domains with MED20: view

Function for MED20 Gene

Molecular function for MED20 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Phenotypes From GWAS Catalog for MED20 Gene

Gene Ontology (GO) - Molecular Function for MED20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003712 transcription coregulator activity IEA --
GO:0003713 transcription coactivator activity IBA 21873635
GO:0003899 DNA-directed 5'-3' RNA polymerase activity NAS 9933582
GO:0005515 protein binding IPI 14576168
GO:0061630 ubiquitin protein ligase activity IEA --
genes like me logo Genes that share ontologies with MED20: view
genes like me logo Genes that share phenotypes with MED20: view

Animal Model Products

CRISPR Products

miRNA for MED20 Gene

miRTarBase miRNAs that target MED20

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED20

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MED20 Gene

Localization for MED20 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED20 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED20 gene
Compartment Confidence
nucleus 5
extracellular 3
cytoskeleton 2
mitochondrion 2
plasma membrane 1
peroxisome 1
endoplasmic reticulum 1
endosome 1
cytosol 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Centrosome (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MED20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IEA --
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
GO:0016592 mediator complex NAS,IEA 9933582
genes like me logo Genes that share ontologies with MED20: view

Pathways & Interactions for MED20 Gene

genes like me logo Genes that share pathways with MED20: view

Pathways by source for MED20 Gene

1 GeneGo (Thomson Reuters) pathway for MED20 Gene
  • Transcription Ligand-Dependent Transcription of Retinoid-Target genes

Gene Ontology (GO) - Biological Process for MED20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated NAS 9933582
GO:0006357 regulation of transcription by RNA polymerase II IEA,IBA 21873635
GO:0006366 transcription by RNA polymerase II TAS 9933582
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with MED20: view

No data available for SIGNOR curated interactions for MED20 Gene

Drugs & Compounds for MED20 Gene

No Compound Related Data Available

Transcripts for MED20 Gene

mRNA/cDNA for MED20 Gene

4 REFSEQ mRNAs :
18 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED20

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for MED20 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c · 7d
SP1: - -
SP2: - - - -
SP3: - - - - -
SP4: - - - - -
SP5: - - -
SP6: -

Relevant External Links for MED20 Gene

GeneLoc Exon Structure for
MED20

Expression for MED20 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MED20 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for MED20 Gene

This gene is overexpressed in Bone (16.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MED20 Gene



Protein tissue co-expression partners for MED20 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MED20

SOURCE GeneReport for Unigene cluster for MED20 Gene:

Hs.278434

Evidence on tissue expression from TISSUES for MED20 Gene

  • Liver(4.2)
  • Nervous system(3.4)
genes like me logo Genes that share expression patterns with MED20: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED20 Gene

Orthologs for MED20 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED20 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED20 31 30
  • 99.69 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MED20 31
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED20 31 30
  • 92.92 (n)
OneToOne
cow
(Bos Taurus)
Mammalia MED20 31 30
  • 92.61 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Med20 30
  • 91.67 (n)
mouse
(Mus musculus)
Mammalia Med20 17 31 30
  • 91.19 (n)
Gm20517 31
  • 91 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia MED20 31
  • 80 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED20 31 30
  • 78.2 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 31
  • 85 (a)
OneToMany
-- 31
  • 80 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia med20 30
  • 73.78 (n)
Str.17211 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.15205 30
zebrafish
(Danio rerio)
Actinopterygii med20 31 30
  • 66.35 (n)
OneToOne
Dr.23717 30
fruit fly
(Drosophila melanogaster)
Insecta MED20 31 30
  • 51.41 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta MED20_ANOGA 30
  • 50.49 (n)
worm
(Caenorhabditis elegans)
Secernentea mdt-20 31 30
  • 47.7 (n)
OneToOne
Species where no ortholog for MED20 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MED20 Gene

ENSEMBL:
Gene Tree for MED20 (if available)
TreeFam:
Gene Tree for MED20 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MED20: view image

Paralogs for MED20 Gene

(1) SIMAP similar genes for MED20 Gene using alignment to 5 proteins:

  • MED20_HUMAN
  • A6PVP4_HUMAN
  • B4DE08_HUMAN
  • B7ZBQ1_HUMAN
  • B7ZBQ3_HUMAN
genes like me logo Genes that share paralogs with MED20: view

No data available for Paralogs for MED20 Gene

Variants for MED20 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MED20 Gene

SNP ID Clinical significance and condition Chr 06 pos Variation AA Info Type
rs730882147 Uncertain Significance: not provided 41,909,351(-) C/G MISSENSE_VARIANT
rs759721745 Uncertain Significance: not provided 41,909,439(-) G/A MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for MED20 Gene

Structural Variations from Database of Genomic Variants (DGV) for MED20 Gene

Variant ID Type Subtype PubMed ID
nsv5278 CNV insertion 18451855
nsv830646 CNV loss 17160897

Variation tolerance for MED20 Gene

Residual Variation Intolerance Score: 34.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.19; 4.32% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MED20 Gene

Human Gene Mutation Database (HGMD)
MED20
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED20

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED20 Gene

Disorders for MED20 Gene

MalaCards: The human disease database

(2) MalaCards diseases for MED20 Gene - From: DISEASES

Disorder Aliases PubMed IDs
waardenburg syndrome, type 3
  • ws3
waardenburg syndrome, type 1
  • ws1
- elite association - COSMIC cancer census association via MalaCards
Search MED20 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for MED20

genes like me logo Genes that share disorders with MED20: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED20 Gene

Publications for MED20 Gene

  1. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S … Conaway RC (Molecular cell 2004) 2 3 4 54
  2. The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex. (PMID: 9933582) Xiao H … Roeder RG (The Journal of biological chemistry 1999) 2 3 4 54
  3. MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X … Roeder RG (Molecular cell 2005) 3 4 54
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 54

Products for MED20 Gene

Sources for MED20 Gene