Aliases for MED16 Gene

Aliases for MED16 Gene

  • Mediator Complex Subunit 16 2 3 4 5
  • DRIP92 2 3 4
  • Thyroid Hormone Receptor-Associated Protein Complex 95 KDa Component 3 4
  • Vitamin D3 Receptor-Interacting Protein Complex 92 KDa Component 3 4
  • Mediator Of RNA Polymerase II Transcription Subunit 16 3 4
  • Thyroid Hormone Receptor-Associated Protein 5 3 4
  • THRAP5 3 4
  • TRAP95 2 3
  • Thyroid Hormone Receptor-Associated Protein, 95-KD Subunit 3
  • Thyroid Hormone Receptor Associated Protein 5 2
  • Trap95 4
  • MED16 5

External Ids for MED16 Gene

Previous HGNC Symbols for MED16 Gene

  • THRAP5

Previous GeneCards Identifiers for MED16 Gene

  • GC19M000819
  • GC19M000640
  • GC19M000884
  • GC19M000889
  • GC19M000906
  • GC19M000868

Summaries for MED16 Gene

GeneCards Summary for MED16 Gene

MED16 (Mediator Complex Subunit 16) is a Protein Coding gene. Diseases associated with MED16 include Retinitis Pigmentosa 20. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Thyroid hormone signaling pathway. Gene Ontology (GO) annotations related to this gene include transcription coregulator activity.

UniProtKB/Swiss-Prot Summary for MED16 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Wiki entry for MED16 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MED16 Gene

Genomics for MED16 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for MED16 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J000891 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 615.5 +0.4 410 2.6 GATAD2A TEAD4 ZNF629 IKZF1 NFKBIZ ZNF692 POLR2A BACH1 JUND LARP7 MED16 RNU6-9 lnc-R3HDM4-1 lnc-R3HDM4-3 piR-58632 WDR18 CFD
GH19J000885 Promoter 0.5 EPDnew 600.4 +7.2 7229 0.1 RBFOX2 TARDBP MED16 lnc-CFD-1 lnc-R3HDM4-3 CFD
GH19J001236 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 46.5 -363.5 -363475 41.4 ZNF221 ARHGAP35 SP1 HNRNPL CREB1 GATAD2A CTCF ZBTB33 ATF7 TEAD4 CIRBP FAM174C MIDN CBARP ATP5F1D SF3A2 TMEM259 TCF3 LOC100288123 PTBP1
GH19J001382 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 45.9 -490.9 -490934 2.5 ZNF221 BRCA1 SP1 HNRNPL CREB1 GATAD2A ATF7 TEAD4 PRDM10 ZNF629 NONHSAG024361.2 NDUFS7 TRN-GTT2-6 TRF-GAA1-6 piR-32939 piR-33470-012 CIRBP SF3A2 TMEM259 TCF3
GH19J001019 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 38.3 -131.8 -131821 12.5 ZNF221 ARHGAP35 SP1 HNRNPL CREB1 GATAD2A ATF7 TEAD4 PRDM10 ZNF629 TMEM259 CNN2 GRIN3B DAZAP1 CIRBP MIER2 LOC100288123 TCF3 PTBP1 ABCA7
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MED16 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MED16

Top Transcription factor binding sites by QIAGEN in the MED16 gene promoter:
  • AP-2alpha
  • AP-2alphaA
  • AP-2beta
  • AP-2gamma
  • ATF6
  • E47
  • MIF-1
  • NF-1
  • NF-1/L
  • TBP

Genomic Locations for MED16 Gene

Genomic Locations for MED16 Gene
chr19:867,630-893,228
(GRCh38/hg38)
Size:
25,599 bases
Orientation:
Minus strand
chr19:867,962-893,218
(GRCh37/hg19)
Size:
25,257 bases
Orientation:
Minus strand

Genomic View for MED16 Gene

Genes around MED16 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED16 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED16 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED16 Gene

Proteins for MED16 Gene

  • Protein details for MED16 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y2X0-MED16_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 16
    Protein Accession:
    Q9Y2X0
    Secondary Accessions:
    • Q6PJT2
    • Q96AD4
    • Q96I35
    • Q9Y652

    Protein attributes for MED16 Gene

    Size:
    877 amino acids
    Molecular mass:
    96793 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
    SequenceCaution:
    • Sequence=AAD31087.1; Type=Frameshift; Evidence={ECO:0000305};

    Alternative splice isoforms for MED16 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED16 Gene

Post-translational modifications for MED16 Gene

  • Ubiquitination at Lys26, Lys149, Lys158, Lys252, Lys284, Lys338, Lys574, Lys745, Lys808, and Lys817
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for MED16 Gene

Domains & Families for MED16 Gene

Gene Families for MED16 Gene

Suggested Antigen Peptide Sequences for MED16 Gene

GenScript: Design optimal peptide antigens:
  • Mediator complex subunit MED16 variant MED16_i2 (B9TWZ5_HUMAN)
  • Mediator complex subunit MED16 variant MED16_i4 (B9TWZ7_HUMAN)
  • Mediator complex subunit MED16 variant MED16_i5 (B9TWZ8_HUMAN)
  • Mediator complex subunit MED16 variant MED16_i6 (B9TWZ9_HUMAN)
  • Mediator complex subunit MED16 variant MED16_i7 (B9TX00_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9Y2X0

UniProtKB/Swiss-Prot:

MED16_HUMAN :
  • Belongs to the Mediator complex subunit 16 family.
Family:
  • Belongs to the Mediator complex subunit 16 family.
genes like me logo Genes that share domains with MED16: view

Function for MED16 Gene

Molecular function for MED16 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Phenotypes From GWAS Catalog for MED16 Gene

Gene Ontology (GO) - Molecular Function for MED16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003712 transcription coregulator activity IDA 12218053
GO:0003713 transcription coactivator activity TAS 10198638
GO:0003824 catalytic activity IEA --
GO:0005515 protein binding IEA,IPI 24882805
GO:0030375 thyroid hormone receptor coactivator activity IDA 10198638
genes like me logo Genes that share ontologies with MED16: view
genes like me logo Genes that share phenotypes with MED16: view

Animal Models for MED16 Gene

MGI Knock Outs for MED16:
  • Med16 Med16<tm1b(KOMP)Wtsi>

Animal Model Products

CRISPR Products

miRNA for MED16 Gene

miRTarBase miRNAs that target MED16

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED16

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MED16 Gene

Localization for MED16 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED16 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED16 gene
Compartment Confidence
nucleus 5
plasma membrane 1
extracellular 1
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for MED16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,NAS 10198638
GO:0005654 nucleoplasm TAS --
GO:0016020 membrane HDA 19946888
GO:0016592 mediator complex IEA,IDA 10198638
genes like me logo Genes that share ontologies with MED16: view

Pathways & Interactions for MED16 Gene

genes like me logo Genes that share pathways with MED16: view

Pathways by source for MED16 Gene

1 GeneGo (Thomson Reuters) pathway for MED16 Gene
  • Transcription Ligand-Dependent Transcription of Retinoid-Target genes
1 Qiagen pathway for MED16 Gene
  • Glucocorticoid Receptor Signaling

Gene Ontology (GO) - Biological Process for MED16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006357 regulation of transcription by RNA polymerase II NAS 10198638
GO:0006366 transcription by RNA polymerase II TAS 10198638
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0045893 positive regulation of transcription, DNA-templated IBA,IDA 10198638
genes like me logo Genes that share ontologies with MED16: view

No data available for SIGNOR curated interactions for MED16 Gene

Drugs & Compounds for MED16 Gene

No Compound Related Data Available

Transcripts for MED16 Gene

mRNA/cDNA for MED16 Gene

1 REFSEQ mRNAs :
62 NCBI additional mRNA sequence :
14 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED16

Alternative Splicing Database (ASD) splice patterns (SP) for MED16 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^
SP1: - - -
SP2: - - -
SP3: - - - -
SP4: - - -
SP5: - - - -
SP6: - - - - - - - - - - - - -
SP7: - -
SP8:

ExUns: 19 ^ 20a · 20b
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:

Relevant External Links for MED16 Gene

GeneLoc Exon Structure for
MED16

Expression for MED16 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MED16 Gene

Protein differential expression in normal tissues from HIPED for MED16 Gene

This gene is overexpressed in Platelet (54.9) and Breast (9.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MED16 Gene



Protein tissue co-expression partners for MED16 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MED16

SOURCE GeneReport for Unigene cluster for MED16 Gene:

Hs.365207

Evidence on tissue expression from TISSUES for MED16 Gene

  • Nervous system(3.2)
  • Muscle(2.5)
genes like me logo Genes that share expression patterns with MED16: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED16 Gene

Orthologs for MED16 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED16 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia MED16 30 31
  • 97.92 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia MED16 30 31
  • 89.42 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia MED16 30 31
  • 88.14 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Med16 30
  • 86.02 (n)
Mouse
(Mus musculus)
Mammalia Med16 30 17 31
  • 85.65 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia MED16 31
  • 85 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia MED16 31
  • 55 (a)
OneToOne
Chicken
(Gallus gallus)
Aves MED16 30 31
  • 78.23 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia MED16 31
  • 88 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia med16 30
  • 71.46 (n)
Str.15997 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.16367 30
Zebrafish
(Danio rerio)
Actinopterygii med16 30 31
  • 71.36 (n)
OneToOne
wufc29h12 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1586 30
Fruit Fly
(Drosophila melanogaster)
Insecta MED16 30 31
  • 45.75 (n)
OneToOne
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 38 (a)
OneToMany
-- 31
  • 35 (a)
OneToMany
Species where no ortholog for MED16 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for MED16 Gene

ENSEMBL:
Gene Tree for MED16 (if available)
TreeFam:
Gene Tree for MED16 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MED16: view image

Paralogs for MED16 Gene

No data available for Paralogs for MED16 Gene

Variants for MED16 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MED16 Gene

SNP ID Clinical significance and condition Chr 19 pos Variation AA Info Type
747218 Likely Benign: not provided 885,760(-) G/A INTRON_VARIANT
778799 Likely Benign: not provided 879,947(-) C/T MISSENSE_VARIANT
780494 Benign: not provided 868,421(-) G/A SYNONYMOUS_VARIANT
788007 Benign: not provided 875,245(-) G/A SYNONYMOUS_VARIANT
789107 Benign: not provided 886,082(-) G/A SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for MED16 Gene

Structural Variations from Database of Genomic Variants (DGV) for MED16 Gene

Variant ID Type Subtype PubMed ID
dgv134n111 CNV deletion 26073780
dgv1681n106 CNV deletion 24896259
dgv6177n54 CNV loss 21841781
dgv6178n54 CNV loss 21841781
dgv6179n54 CNV loss 21841781
esv1010169 CNV insertion 20482838
esv2273959 CNV deletion 18987734
esv2670297 CNV deletion 23128226
esv2717801 CNV deletion 23290073
esv2717802 CNV deletion 23290073
esv2717803 CNV deletion 23290073
esv2717804 CNV deletion 23290073
esv2717805 CNV deletion 23290073
esv2717808 CNV deletion 23290073
esv2717809 CNV deletion 23290073
esv2717810 CNV deletion 23290073
esv28908 CNV gain 19812545
esv3331953 CNV duplication 20981092
esv3399640 CNV duplication 20981092
esv3473 CNV loss 18987735
esv3555830 CNV deletion 23714750
esv3643375 CNV gain 21293372
nsv1071885 CNV deletion 25765185
nsv1135520 CNV deletion 24896259
nsv1143892 CNV deletion 24896259
nsv1146217 CNV deletion 26484159
nsv1160555 CNV deletion 26073780
nsv2385 CNV insertion 18451855
nsv470101 CNV loss 18288195
nsv513505 CNV insertion 21212237
nsv578175 CNV gain+loss 21841781
nsv578176 CNV loss 21841781
nsv578177 CNV gain+loss 21841781
nsv953938 CNV deletion 24416366

Variation tolerance for MED16 Gene

Residual Variation Intolerance Score: 1.25% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.94; 67.90% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MED16 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED16

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED16 Gene

Disorders for MED16 Gene

MalaCards: The human disease database

(1) MalaCards diseases for MED16 Gene - From: DISEASES

Disorder Aliases PubMed IDs
retinitis pigmentosa 20
  • rp20
- elite association - COSMIC cancer census association via MalaCards
Search MED16 in MalaCards View complete list of genes associated with diseases
genes like me logo Genes that share disorders with MED16: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED16 Gene

Publications for MED16 Gene

  1. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. (PMID: 10198638) Ito M … Roeder RG (Molecular cell 1999) 2 3 4
  2. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. (PMID: 10235266) Rachez C … Freedman LP (Nature 1999) 2 3 4
  3. MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X … Roeder RG (Molecular cell 2005) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S … Conaway RC (Molecular cell 2004) 3 4

Products for MED16 Gene

  • Signalway ELISA kits for MED16
  • Signalway Proteins for MED16

Sources for MED16 Gene