The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [pr... See more...

Aliases for MED15 Gene

Aliases for MED15 Gene

  • Mediator Complex Subunit 15 2 3 4 5
  • TIG-1 2 3 4
  • PC2 (Positive Cofactor 2, Multiprotein Complex) Glutamine/Q-Rich-Associated Protein 2 3
  • Mediator Of RNA Polymerase II Transcription Subunit 15 3 4
  • Activator-Recruited Cofactor 105 KDa Component 3 4
  • Trinucleotide Repeat-Containing Gene 7 Protein 3 4
  • PC2 Glutamine/Q-Rich-Associated Protein 3 4
  • Trinucleotide Repeat Containing 7 2 3
  • TPA-Inducible Gene 1 Protein 3 4
  • CTG Repeat Protein 7a 3 4
  • ARC105 3 4
  • CAG7A 2 3
  • CTG7A 3 4
  • PCQAP 3 4
  • TNRC7 3 4
  • TIG1 3 4
  • Positive Cofactor 2, Glutamine/Q-Rich-Associated Protein 3
  • Positive Cofactor 2 Glutamine/Q-Rich-Associated Protein 4
  • PC2-Glutamine-Rich-Associated Protein 3
  • Activator-Recruited Cofactor, 105-KD 3
  • TPA Inducible Protein 3
  • TPA Inducible Gene-1 3
  • Arc105 2
  • MED15 5

External Ids for MED15 Gene

Previous HGNC Symbols for MED15 Gene

  • TNRC7
  • PCQAP

Previous GeneCards Identifiers for MED15 Gene

  • GC22P019191
  • GC22P020850
  • GC22P004129

Summaries for MED15 Gene

Entrez Gene Summary for MED15 Gene

  • The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

GeneCards Summary for MED15 Gene

MED15 (Mediator Complex Subunit 15) is a Protein Coding gene. Diseases associated with MED15 include Digeorge Syndrome and Ureterocele. Among its related pathways are Sterol Regulatory Element-Binding Proteins (SREBP) signalling and DNA Damage/Telomere Stress Induced Senescence.

UniProtKB/Swiss-Prot Summary for MED15 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.

Gene Wiki entry for MED15 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for MED15 Gene

Genomics for MED15 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for MED15 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around MED15 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for MED15

Top Transcription factor binding sites by QIAGEN in the MED15 gene promoter:
  • FOXO4
  • Ik-3
  • Lmo2
  • NRF-2
  • POU2F1
  • POU2F1a
  • SRF
  • SRF (504 AA)
  • STAT3

Genomic Locations for MED15 Gene

Latest Assembly
chr22:20,495,913-20,587,632
(GRCh38/hg38)
Size:
91,720 bases
Orientation:
Plus strand

Previous Assembly
chr22:20,861,897-20,941,906
(GRCh37/hg19 by Entrez Gene)
Size:
80,010 bases
Orientation:
Plus strand

chr22:20,850,200-20,941,919
(GRCh37/hg19 by Ensembl)
Size:
91,720 bases
Orientation:
Plus strand

Genomic View for MED15 Gene

Genes around MED15 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED15 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED15 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED15 Gene

Proteins for MED15 Gene

  • Protein details for MED15 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96RN5-MED15_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 15
    Protein Accession:
    Q96RN5
    Secondary Accessions:
    • D3DX31
    • D3DX32
    • O15413
    • Q6IC31
    • Q8NF16
    • Q96CT0
    • Q96IH7
    • Q9P1T3

    Protein attributes for MED15 Gene

    Size:
    788 amino acids
    Molecular mass:
    86753 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with SMAD2, SMAD3, SREBF1 and SREBF2. Interacts with WWTR1. Interacts with TRIM11.
    SequenceCaution:
    • Sequence=AAC12944.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=BAB85034.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors.; Evidence={ECO:0000305}; Sequence=BAC03446.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MED15 Gene

    Alternative splice isoforms for MED15 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED15 Gene

Post-translational modifications for MED15 Gene

  • Ubiquitinated by TRIM11, leading to proteasomal degradation.
  • Ubiquitination at Lys578
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for MED15 Gene

Domains & Families for MED15 Gene

Gene Families for MED15 Gene

Protein Domains for MED15 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED15 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ58080, highly similar to Positive cofactor 2glutamine/Q-rich-associated protein (B4DGD6_HUMAN)
  • cDNA FLJ59342, highly similar to Positive cofactor 2glutamine/Q-rich-associated protein (B4DM43_HUMAN)
  • Trinucleotide repeat-containing gene 7 protein (MED15_HUMAN)
  • MED15 protein (Q6PKB8_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q96RN5

UniProtKB/Swiss-Prot:

MED15_HUMAN :
  • Belongs to the Mediator complex subunit 15 family.
Family:
  • Belongs to the Mediator complex subunit 15 family.
genes like me logo Genes that share domains with MED15: view

Function for MED15 Gene

Molecular function for MED15 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
UniProtKB/Swiss-Prot Induction:
By 12-O-tetradecanoylphorbol-13-acetate (TPA).

Phenotypes From GWAS Catalog for MED15 Gene

Gene Ontology (GO) - Molecular Function for MED15 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003712 transcription coregulator activity IEA --
GO:0005515 protein binding IPI 16799563
genes like me logo Genes that share ontologies with MED15: view
genes like me logo Genes that share phenotypes with MED15: view

miRNA for MED15 Gene

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED15

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MED15 Gene

Localization for MED15 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED15 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED15 gene
Compartment Confidence
nucleus 5
cytosol 2
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for MED15 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm IDA,TAS --
GO:0005737 cytoplasm IEA --
GO:0016020 membrane HDA 19946888
GO:0016592 mediator complex IEA --
genes like me logo Genes that share ontologies with MED15: view

Pathways & Interactions for MED15 Gene

genes like me logo Genes that share pathways with MED15: view

Pathways by source for MED15 Gene

Gene Ontology (GO) - Biological Process for MED15 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006357 regulation of transcription by RNA polymerase II IEA --
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
genes like me logo Genes that share ontologies with MED15: view

No data available for SIGNOR curated interactions for MED15 Gene

Drugs & Compounds for MED15 Gene

(1) Drugs for MED15 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with MED15: view

Transcripts for MED15 Gene

mRNA/cDNA for MED15 Gene

6 REFSEQ mRNAs :
30 NCBI additional mRNA sequence :
31 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MED15

Alternative Splicing Database (ASD) splice patterns (SP) for MED15 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14a · 14b ^
SP1:
SP2: - - - - - - - - -
SP3: - - - - - - - - -
SP4: - - - - - - - - - -
SP5: - - - - - - - - - - - -
SP6: - - - - - - - - - - -
SP7: - - - - - - - - -
SP8: - - - - - - - - - - -
SP9: - - - - - - - - -
SP10: - - - - - - -
SP11: - - - - - -
SP12: - - - -
SP13: - - -
SP14: - -
SP15: -
SP16: - - - -
SP17:
SP18:
SP19:
SP20:

ExUns: 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b · 18c ^ 19 ^ 20 ^ 21a · 21b ^ 22 ^ 23
SP1: - -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16: -
SP17:
SP18:
SP19: - -
SP20:

Relevant External Links for MED15 Gene

GeneLoc Exon Structure for
MED15

Expression for MED15 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for MED15 Gene

Protein differential expression in normal tissues from HIPED for MED15 Gene

This gene is overexpressed in Placenta (19.9), Plasma (10.0), and CD8 Tcells (8.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MED15 Gene



Protein tissue co-expression partners for MED15 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for MED15

SOURCE GeneReport for Unigene cluster for MED15 Gene:

Hs.517421

mRNA Expression by UniProt/SwissProt for MED15 Gene:

Q96RN5-MED15_HUMAN
Tissue specificity: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.

Evidence on tissue expression from TISSUES for MED15 Gene

  • Nervous system(4.9)
  • Blood(4.5)
  • Kidney(4.4)
  • Liver(4.4)
  • Spleen(4.3)
  • Eye(4.3)
  • Pancreas(3.6)
  • Lung(3.5)
  • Skin(2.4)
  • Intestine(2.4)
  • Muscle(2.4)
genes like me logo Genes that share expression patterns with MED15: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED15 Gene

Orthologs for MED15 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED15 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia MED15 31
  • 99 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia MED15 31
  • 94 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia MED15 31
  • 93 (a)
OneToOne
Mouse
(Mus musculus)
Mammalia Med15 31
  • 91 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 89 (a)
OneToMany
-- 31
  • 61 (a)
OneToMany
-- 31
  • 52 (a)
OneToMany
-- 31
  • 44 (a)
OneToMany
Oppossum
(Monodelphis domestica)
Mammalia MED15 31
  • 74 (a)
OneToOne
Chicken
(Gallus gallus)
Aves MED15 31
  • 65 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia ARC105 30
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia Str.11806 30
Zebrafish
(Danio rerio)
Actinopterygii med15 31
  • 59 (a)
OneToOne
Dr.7497 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.986 30
Fruit Fly
(Drosophila melanogaster)
Insecta MED15 31
  • 31 (a)
OneToOne
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 35 (a)
OneToOne
Species where no ortholog for MED15 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rat (Rattus norvegicus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for MED15 Gene

ENSEMBL:
Gene Tree for MED15 (if available)
TreeFam:
Gene Tree for MED15 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for MED15: view image

Paralogs for MED15 Gene

(41) SIMAP similar genes for MED15 Gene using alignment to 19 proteins:

  • MED15_HUMAN
  • B4DM43_HUMAN
  • C9J1I1_HUMAN
  • C9JCQ3_HUMAN
  • C9JEM1_HUMAN
  • C9JGN2_HUMAN
  • C9JJ12_HUMAN
  • C9JLN7_HUMAN
  • C9JM58_HUMAN
  • C9JWK5_HUMAN
  • C9JZV5_HUMAN
  • F2Z2B7_HUMAN
  • F5GZU5_HUMAN
  • F8WCS1_HUMAN
  • F8WDM6_HUMAN
  • F8WEJ4_HUMAN
  • G3V1P5_HUMAN
  • H7C308_HUMAN
  • Q6PKB8_HUMAN
genes like me logo Genes that share paralogs with MED15: view

No data available for Paralogs for MED15 Gene

Variants for MED15 Gene

Polymorphic Variants from UniProtKB/Swiss-Prot for MED15 Gene

MED15_HUMAN-Q96RN5
The poly-Gln region from amino acids 235-262 of PCQAP is polymorphic. There are from 15 to 18 repeats in the Italian population.

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for MED15 Gene

SNP ID Clinical significance and condition Chr 22 pos Variation AA Info Type
rs1453731006 Uncertain Significance: Hirschsprung disease 20,566,719(+) C/A
NM_001003891.3(MED15):c.943C>A (p.Pro315Thr)
MISSENSE
rs148534902 Benign: not provided 20,537,211(+) C/CCCTA
NM_001003891.3(MED15):c.156+8_156+11dup
INTRON
rs766191914 Likely Benign: not provided 20,566,679(+) G/A
NM_001003891.3(MED15):c.903G>A (p.Pro301=)
SYNONYMOUS

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for MED15 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for MED15 Gene

Variant ID Type Subtype PubMed ID
dgv4483n100 CNV gain 25217958
dgv7993n54 CNV gain 21841781
dgv7994n54 CNV gain 21841781
esv25802 CNV gain 19812545
esv2763693 CNV gain 21179565
esv3647294 CNV loss 21293372
esv3647295 CNV loss 21293372
esv3893438 CNV gain 25118596
nsv1056614 CNV loss 25217958
nsv1062650 CNV gain 25217958
nsv3561 CNV insertion 18451855
nsv3562 CNV deletion 18451855
nsv436864 CNV insertion 17901297
nsv509810 CNV insertion 20534489
nsv523162 CNV loss 19592680
nsv588293 CNV gain 21841781
nsv588298 CNV gain 21841781
nsv819580 CNV gain 19587683
nsv821342 CNV deletion 20802225
nsv828954 CNV gain 20364138
nsv828956 CNV gain 20364138
nsv834136 CNV loss 17160897
nsv834138 CNV loss 17160897
nsv834139 CNV loss 17160897
nsv953033 CNV deletion 24416366

Variation tolerance for MED15 Gene

Residual Variation Intolerance Score: 15.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.14; 38.87% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MED15 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED15

SNP Genotyping and Copy Number Assays for research

Disorders for MED15 Gene

MalaCards: The human disease database

(6) MalaCards diseases for MED15 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
digeorge syndrome
  • dgs
ureterocele
noonan syndrome 5
  • ns5
velocardiofacial syndrome
  • vcfs
patent foramen ovale
  • atrial septal defect within oval fossa
- elite association - COSMIC cancer census association via MalaCards
Search MED15 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for MED15

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with MED15: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED15 Gene

Publications for MED15 Gene

  1. Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit. (PMID: 11414760) Berti L … Meisterernst M (Genomics 2001) 2 3 4 23
  2. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. (PMID: 16799563) Yang F … Näär AM (Nature 2006) 3 4 23
  3. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S … Conaway RC (Molecular cell 2004) 2 3 4
  4. Association study between CAG trinucleotide repeats in the PCQAP gene (PC2 glutamine/Q-rich-associated protein) and schizophrenia. (PMID: 12497610) De Luca A … Novelli G (American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 2003) 3 23 41
  5. A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues. (PMID: 11024300) Abraham S … Solomon WB (Gene 2000) 2 3 4

Products for MED15 Gene

Sources for MED15 Gene