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Aliases for MARCH4 Gene

Aliases for MARCH4 Gene

  • Membrane Associated Ring-CH-Type Finger 4 2 3 5
  • Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase 2 3
  • Membrane-Associated RING Finger Protein 4 3 4
  • RING-Type E3 Ubiquitin Transferase MARCH4 3 4
  • Membrane-Associated RING-CH Protein IV 3 4
  • RING Finger Protein 174 3 4
  • MARCH-IV 3 4
  • RNF174 3 4
  • Membrane-Associated Ring Finger (C3HC4) 4 2
  • E3 Ubiquitin-Protein Ligase MARCH4 3
  • Membrane Associated Ring Finger 4 3
  • EC 4
  • KIAA1399 4

External Ids for MARCH4 Gene

Previous GeneCards Identifiers for MARCH4 Gene

  • GC02M216949
  • GC02M216830
  • GC02M217122
  • GC02M208976

Summaries for MARCH4 Gene

Entrez Gene Summary for MARCH4 Gene

  • MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

GeneCards Summary for MARCH4 Gene

MARCH4 (Membrane Associated Ring-CH-Type Finger 4) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is MARCH9.

UniProtKB/Swiss-Prot for MARCH4 Gene

  • E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Additional gene information for MARCH4 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MARCH4 Gene

Genomics for MARCH4 Gene

GeneHancer (GH) Regulatory Elements for MARCH4 Gene

Promoters and enhancers for MARCH4 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH02I216370 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 550.8 -0.6 -647 3.7 MLX ZFP64 ARID4B SIN3A ZNF2 ZBTB7B SLC30A9 ZNF766 ZNF143 FOS MARCH4 SMARCAL1
GH02I216315 Enhancer 1.2 FANTOM5 Ensembl ENCODE 16.7 +55.7 55716 2.5 JUN ETV1 ZIC2 FOSL1 ZNF316 POLR2A RCOR1 FOSL2 FOS NFE2 MARCH4 ENSG00000233581 GC02P216342 GC02P216343
GH02I216339 Enhancer 1.3 FANTOM5 Ensembl ENCODE dbSUPER 13.7 +29.0 28972 7.7 KLF1 PKNOX1 FOXA2 ZIC2 ETS1 ZFHX2 EGR1 POLR2A EGR2 REST GC02P216342 GC02P216343 MARCH4 SMARCAL1 ENSG00000235042 GC02P216352
GH02I216308 Enhancer 0.9 FANTOM5 ENCODE 15.9 +63.4 63362 1.2 MEIS2 PKNOX1 ATF1 ZBTB21 ZNF189 OVOL3 TSHZ1 ZNF843 ATF2 OSR2 MARCH4 ENSG00000231092 SMARCAL1 ENSG00000235042 ENSG00000233581 GC02P216342 GC02P216343
GH02I216348 Enhancer 1.1 Ensembl ENCODE dbSUPER 12.1 +20.1 20138 5.9 MXI1 KLF1 USF1 NFIB JUN HIC1 FOSL1 ZFHX2 POLR2A VEZF1 ENSG00000207303 GC02P216352 MARCH4 SMARCAL1 ENSG00000235042
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around MARCH4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the MARCH4 gene promoter:

Genomic Locations for MARCH4 Gene

Genomic Locations for MARCH4 Gene
114,166 bases
Minus strand

Genomic View for MARCH4 Gene

Genes around MARCH4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MARCH4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MARCH4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MARCH4 Gene

Proteins for MARCH4 Gene

  • Protein details for MARCH4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    E3 ubiquitin-protein ligase MARCH4
    Protein Accession:
    Secondary Accessions:
    • Q4KMN7
    • Q86WR8

    Protein attributes for MARCH4 Gene

    410 amino acids
    Molecular mass:
    45528 Da
    Quaternary structure:
    No Data Available
    • Sequence=BAA92637.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for MARCH4 Gene

Post-translational modifications for MARCH4 Gene

No Post-translational modifications

Other Protein References for MARCH4 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for MARCH4 Gene

Domains & Families for MARCH4 Gene

Gene Families for MARCH4 Gene

Protein Domains for MARCH4 Gene

Graphical View of Domain Structure for InterPro Entry



  • The RING-CH-type zinc finger domain is required for E3 ligase activity.
  • The RING-CH-type zinc finger domain is required for E3 ligase activity.
genes like me logo Genes that share domains with MARCH4: view

No data available for Suggested Antigen Peptide Sequences for MARCH4 Gene

Function for MARCH4 Gene

Molecular function for MARCH4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Enzyme Numbers (IUBMB) for MARCH4 Gene

Phenotypes From GWAS Catalog for MARCH4 Gene

Gene Ontology (GO) - Molecular Function for MARCH4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IDA 14722266
GO:0008270 zinc ion binding IEA --
GO:0016740 transferase activity IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with MARCH4: view
genes like me logo Genes that share phenotypes with MARCH4: view

Animal Model Products

Inhibitory RNA Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MARCH4 Gene

Localization for MARCH4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MARCH4 Gene

Golgi apparatus membrane; Multi-pass membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MARCH4 gene
Compartment Confidence
golgi apparatus 5
plasma membrane 2
extracellular 2
mitochondrion 1
cytosol 1
lysosome 1

Gene Ontology (GO) - Cellular Components for MARCH4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0005794 Golgi apparatus IEA --
GO:0005795 Golgi stack IDA 14722266
GO:0005802 trans-Golgi network IDA 14722266
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with MARCH4: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for MARCH4 Gene

Pathways & Interactions for MARCH4 Gene

SuperPathways for MARCH4 Gene

No Data Available

UniProtKB/Swiss-Prot Q9P2E8-MARH4_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for MARCH4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with MARCH4: view

No data available for Pathways by source and SIGNOR curated interactions for MARCH4 Gene

Drugs & Compounds for MARCH4 Gene

(3) Drugs for MARCH4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
ATP Investigational Nutra Agonist 0

(1) Additional Compounds for MARCH4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
genes like me logo Genes that share compounds with MARCH4: view

Transcripts for MARCH4 Gene

mRNA/cDNA for MARCH4 Gene

(1) REFSEQ mRNAs :
(5) Additional mRNA sequences :
(40) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for MARCH4 Gene

Membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase:
Representative Sequences:

Inhibitory RNA Products

Alternative Splicing Database (ASD) splice patterns (SP) for MARCH4 Gene

No ASD Table

Relevant External Links for MARCH4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for MARCH4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MARCH4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MARCH4 Gene

This gene is overexpressed in Brain - Nucleus accumbens (basal ganglia) (x5.1), Brain - Cortex (x4.8), Brain - Anterior cingulate cortex (BA24) (x4.2), and Pituitary (x4.1).

Protein differential expression in normal tissues from HIPED for MARCH4 Gene

This gene is overexpressed in Adrenal (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for MARCH4 Gene

Protein tissue co-expression partners for MARCH4 Gene

- Elite partner

SOURCE GeneReport for Unigene cluster for MARCH4 Gene:


mRNA Expression by UniProt/SwissProt for MARCH4 Gene:

Tissue specificity: Expressed in brain and placenta.

Evidence on tissue expression from TISSUES for MARCH4 Gene

  • Nervous system(4.2)
  • Liver(4.1)
  • Pancreas(4.1)
genes like me logo Genes that share expression patterns with MARCH4: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for MARCH4 Gene

Orthologs for MARCH4 Gene

This gene was present in the common ancestor of chordates.

Orthologs for MARCH4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia MARCH4 33 34
  • 100 (n)
(Bos Taurus)
Mammalia MARCH4 33 34
  • 91.58 (n)
(Canis familiaris)
Mammalia MARCH4 33 34
  • 91.54 (n)
(Rattus norvegicus)
Mammalia March4 33
  • 88.77 (n)
(Mus musculus)
Mammalia March4 33 16 34
  • 88.72 (n)
(Monodelphis domestica)
Mammalia MARCH4 34
  • 81 (a)
(Gallus gallus)
Aves MARCH4 34
  • 80 (a)
MAR-04 33
  • 77.91 (n)
(Anolis carolinensis)
Reptilia MARCH4 34
  • 79 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia march4 33
  • 71.31 (n)
(Danio rerio)
Actinopterygii zgc:153256 33
  • 64.28 (n)
march4 34
  • 55 (a)
MARCH4 (1 of 2) 34
  • 52 (a)
Dr.18480 33
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 27 (a)
Species where no ortholog for MARCH4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MARCH4 Gene

Gene Tree for MARCH4 (if available)
Gene Tree for MARCH4 (if available)

Paralogs for MARCH4 Gene

Paralogs for MARCH4 Gene

(2) SIMAP similar genes for MARCH4 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with MARCH4: view

Variants for MARCH4 Gene

Sequence variations from dbSNP and Humsavar for MARCH4 Gene

SNP ID Clin Chr 02 pos Variation AA Info Type
rs1000008380 -- 216,333,793(-) G/A intron_variant
rs1000063618 -- 216,335,058(-) T/C intron_variant
rs1000064920 -- 216,323,559(-) G/T intron_variant
rs1000115392 -- 216,283,995(-) C/T intron_variant
rs1000122748 -- 216,289,662(-) T/C intron_variant

Structural Variations from Database of Genomic Variants (DGV) for MARCH4 Gene

Variant ID Type Subtype PubMed ID
nsv999217 CNV gain 25217958
nsv955059 CNV deletion 24416366
nsv834537 CNV loss 17160897
nsv507067 OTHER sequence alteration 20534489
nsv3160 CNV deletion 18451855
nsv1148113 CNV deletion 26484159
nsv1011506 CNV gain 25217958
nsv1005697 CNV loss 25217958
nsv1003877 CNV gain 25217958
nsv1001603 CNV gain 25217958
esv3594292 CNV loss 21293372
esv3560987 CNV deletion 23714750
esv2721460 CNV deletion 23290073
esv2721458 CNV deletion 23290073
esv2678065 CNV deletion 23128226
esv1006636 CNV deletion 20482838
dgv630e201 CNV deletion 23290073

Variation tolerance for MARCH4 Gene

Residual Variation Intolerance Score: 45.8% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for MARCH4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MARCH4 Gene

Disorders for MARCH4 Gene

Additional Disease Information for MARCH4

No disorders were found for MARCH4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MARCH4 Gene

Publications for MARCH4 Gene

  1. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. (PMID: 14722266) Bartee E … Früh K (Journal of virology 2004) 2 3 4 22 58
  2. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. (PMID: 10718198) Nagase T … Ohara O (DNA research : an international journal for rapid publication of reports on genes and genomes 2000) 2 3 4 58
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58
  4. Genome-wide association analysis identifies susceptibility loci for migraine without aura. (PMID: 22683712) Freilinger T … International Headache Genetics Consortium (Nature genetics 2012) 3 58
  5. Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase. (PMID: 20870941) Nice TJ … Raulet DH (Journal of immunology (Baltimore, Md. : 1950) 2010) 3 58

Products for MARCH4 Gene

Sources for MARCH4 Gene

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