Aliases for MAP2K4 Gene
Aliases for MAP2K4 Gene
External Ids for MAP2K4 Gene
- HGNC: 6844
- Entrez Gene: 6416
- Ensembl: ENSG00000065559
- OMIM: 601335
- UniProtKB: P45985
Previous HGNC Symbols for MAP2K4 Gene
- SERK1
Previous GeneCards Identifiers for MAP2K4 Gene
- GC17P012290
- GC17P013039
- GC17P011868
- GC17P012124
- GC17P011864
Summaries for MAP2K4 Gene
-
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
GeneCards Summary for MAP2K4 Gene
MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4) is a Protein Coding gene. Diseases associated with MAP2K4 include Cerebral Cavernous Malformations 2. Among its related pathways are p38 MAPK Signaling Pathway (WikiPathways) and Immune response Fc epsilon RI pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAP2K6.
UniProtKB/Swiss-Prot for MAP2K4 Gene
-
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
Additional gene information for MAP2K4 Gene
No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MAP2K4 Gene
Genomics for MAP2K4 Gene
GeneHancer (GH) Regulatory Elements for MAP2K4 Gene
Promoters and enhancers for MAP2K4 Gene
| GeneHancer (GH) Identifier | GH Type | GH Score |
GH Sources | Gene Association Score | Total Score | TSS distance (kb) | Number of Genes Away | Size (kb) | Transcription Factor Binding Sites |
Gene Targets |
|---|---|---|---|---|---|---|---|---|---|---|
| GH17J012020 | Promoter/Enhancer | 1.9 | EPDnew Ensembl ENCODE | 660.3 | +0.4 | 385 | 2 | ARNT SIN3A ZNF48 YY1 ZNF143 ATF7 SP3 SP5 ZNF592 BRD4 | MAP2K4 RPL21P122 GC17P012021 | |
| GH17J011996 | Promoter/Enhancer | 2 | EPDnew Ensembl ENCODE | 7.8 | -23.5 | -23510 | 2 | HDGF PKNOX1 ARID4B SIN3A DMAP1 YBX1 ZNF2 YY1 POLR2B ZNF213 | ZNF18 MAP2K4 GC17M011978 | |
| GH17J012041 | Enhancer | 0.8 | Ensembl ENCODE | 11.6 | +20.7 | 20683 | 0.6 | FOSL1 JUND POLR2A JUN REST FOSL2 FOS PRDM1 | MAP2K4 RNU11-2P GC17P012021 MIR744 | |
| GH17J011987 | Enhancer | 0.5 | ENCODE | 17.8 | -33.7 | -33677 | 0.1 | CTCF ZNF654 SMC3 REST KDM1A RAD21 PRDM1 | MAP2K4 GC17M011978 ZNF18 | |
| GH17J011992 | Promoter | 0.5 | EPDnew | 12 | -27.8 | -27810 | 0.1 | ZNF18 MAP2K4 GC17M011978 |
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)
GeneHancers around MAP2K4 on UCSC Golden Path with GeneCards custom track
- Top Transcription factor binding sites by QIAGEN in the MAP2K4 gene promoter:
-
- AP-1
- c-Jun
- ATF-2
- ER-alpha
- AML1a
- NF-E2 p45
- NF-E2
- GR
- GR-alpha
- CUTL1
Regulatory Element Products
Genomic Locations for MAP2K4 Gene
Genomic Locations for MAP2K4 Gene
- chr17:12,020,818-12,143,831
- (GRCh38/hg38)
- Size:
- 123,014 bases
- Orientation:
- Plus strand
- chr17:11,924,135-12,047,147
- (GRCh37/hg19)
- Size:
- 123,013 bases
- Orientation:
- Plus strand
Genomic View for MAP2K4 Gene
Genes around MAP2K4 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 17p12 by Entrez Gene
- 17p12 by HGNC
- 17p12 by Ensembl
MAP2K4 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for MAP2K4 Gene
Proteins for MAP2K4 Gene
-
Protein details for MAP2K4 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P45985-MP2K4_HUMAN
- Recommended name:
- Dual specificity mitogen-activated protein kinase kinase 4
- Protein Accession:
- P45985
- B2R7N7
- B3KYB2
- D3DTS5
- Q5U0B8
- Q6FHX4
- Q6P9H2
- Q6PIE6
Protein attributes for MAP2K4 Gene
- Size:
- 399 amino acids
- Molecular mass:
- 44288 Da
- Quaternary structure:
-
- Interacts with SPAG9 (By similarity). Interacts (via its D domain) with its substrates MAPK8/JNK1, MAPK9/JNK2, MAPK10/JNK3, MAPK11 and MAPK14. Interacts (via its DVD domain) with MAP3Ks activators like MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with ARRB1, ARRB2 and MAPK8IP3/JIP3.
Protein Expression for MAP2K4 Gene
Post-translational modifications for MAP2K4 Gene
- Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks).
- Modification sites at PhosphoSitePlus
- Modification sites at neXtProt
Other Protein References for MAP2K4 Gene
Antibody Products
- R&D Systems Antibodies for MAP2K4 (MKK4)
-
Custom Antibody ServicesOriGene Antibodies for MAP2K4
- AM06144SU-N
- AM06580SU-N
- AP00207PU-N
- AP01678PU-N
- AP01679PU-N
- AP02453PU-N
- AP02453PU-S
- AP02454PU-N
- AP02454PU-S
- AP02660PU-N
- AP02660PU-S
- AP02661PU-N
- AP02661PU-S
- AP06311PU-N
- AP06312PU-N
- AP20270PU-N
- AP20418PU-N
- CF500403
- CF500411
- CF500412
- CF500428
- CF500430
- TA300116
- TA300580
- TA300743
- TA309998
- TA309999
- TA321550
- TA325642
- TA325643
- TA326264
- TA327868
- TA327914
- TA333238
- TA333239
- TA500403
- TA500411
- TA500412
- TA500428
- TA500430
- Novus Biologicals Antibodies for MAP2K4
-
Abcam antibodies for MAP2K4
- Invitrogen Antibodies for MAP2K4 (AFLGC-MAP2K4)
- antibodies-online: Search results for 338 available MAP2K4 Antibodies ranked by validation data
- Compare Top MAP2K4 Antibodies
-
Recommended
- 83 WB Antibodies (anti-rat)
- 24 IHC Antibodies (anti-rat)
- 12 IF (p) Antibodies (anti-rat)
- 12 IHC (p) Antibodies (anti-rat)
- 1 IP Antibody (anti-rat)
- 223 WB Antibodies (anti-human)
- 41 IHC Antibodies (anti-human)
- 16 IHC (p) Antibodies (anti-human)
- 12 IF (p) Antibodies (anti-human)
- 4 IP Antibodies (anti-human)
- 116 WB Antibodies (anti-mouse)
- 26 IHC Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 12 IHC (p) Antibodies (anti-mouse)
- 1 IP Antibody (anti-mouse)
- GeneTex MAP2K4 Antibody for MAP2K4
-
Santa Cruz Biotechnology (SCBT) Antibodies for MAP2K4
- Sino Biological Antibodies for MAP2K4
Protein Products
-
OriGene Purified Proteins for MAP2K4
- Search Origene for MassSpec and Protein Over-expression Lysates for MAP2K4
- Origene Custom Protein Services for MAP2K4
- Sino Biological Cell Lysates for MAP2K4
- Sino Biological Recombinant Proteins for MAP2K4
- Search GeneTex for Proteins for MAP2K4
-
Abcam proteins for MAP2K4
Assay Products
No data available for DME Specific Peptides for MAP2K4 Gene
Domains & Families for MAP2K4 Gene
Gene Families for MAP2K4 Gene
- HGNC:
- IUPHAR :
- Human Protein Atlas (HPA):
-
- Cancer-related genes
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for MAP2K4 Gene
- InterPro:
- ProtoNet:
Suggested Antigen Peptide Sequences for MAP2K4 Gene
- GenScript: Design optimal peptide antigens:
Graphical View of Domain Structure for InterPro Entry
P45985UniProtKB/Swiss-Prot:
MP2K4_HUMAN :- The DVD domain (residues 364-387) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation.
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Domain:
-
- The DVD domain (residues 364-387) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation.
- The D domain (residues 34-52) contains a conserved docking site and is required for the binding to MAPK substrates.
- Family:
-
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Function for MAP2K4 Gene
Molecular function for MAP2K4 Gene
- UniProtKB/Swiss-Prot Function:
- Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
- UniProtKB/Swiss-Prot CatalyticActivity:
- ATP + a protein = ADP + a phosphoprotein.
- UniProtKB/Swiss-Prot EnzymeRegulation:
- Activated in response to a variety of cellular stresses, including UV and gamma-irradiation, heat shock, hyperosmolarity, T-cell receptor stimulation, peroxide and inflammatory cytokines. Also activated by developmental cues. MAP2K4/MKK4 is activated by the majority of MKKKs, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K7/TAK1, MAP3K10/MLK2, MAP3K11/MLK3, MAP3K12/DLK and MAP3K13/LZK.
- GENATLAS Biochemistry:
- mitogen-activated protein kinase kinase 4,binding to JUN transactivation domain and phosphorylating it,involved in signal transduction,also involved in Ras pathway and hepatogenesis
Enzyme Numbers (IUBMB) for MAP2K4 Gene
Phenotypes From GWAS Catalog for MAP2K4 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000166 | nucleotide binding | IEA | -- |
| GO:0004672 | protein kinase activity | TAS | 7716521 |
| GO:0004674 | protein serine/threonine kinase activity | IBA | -- |
| GO:0004708 | MAP kinase kinase activity | IEA,IBA | -- |
| GO:0004713 | protein tyrosine kinase activity | IEA | -- |
Phenotypes for MAP2K4 Gene
- MGI mutant phenotypes for MAP2K4:
- inferred from 4 alleles
- GenomeRNAi human phenotypes for MAP2K4:
-
- Increased mitotic index
- Decreased substrate adherent cell growth
- Decreased shRNA abundance (Z-score < -2)
- Increased shRNA abundance (Z-score > 2)
- Decreased viability
- Negative genetic interaction between MUS81-/- and MUS81+/+
- Increased colony dispersion (increased number of colonies and decreased number of cells per colony)
- Decreased viability in esophageal squamous lineage
- Increased Nanog expression
- Increased cell viability after pRB stimulation
- no effect
- Decreased human cytomegalovirus (HCMV) strain AD169 replication
- Increased vaccinia virus (VACV) infection
- Resistant to vaccinia virus (VACV-A4L) infection
- Increased viability
Animal Models for MAP2K4 Gene
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
- Cyagen custom Knockout/knockin (KOKI) mouse models for MAP2K4
-
CRISPR Products
-
OriGene CRISPR knockouts for MAP2K4
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for MAP2K4
- genomics-online: gRNA clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- Applied Biological Materials CRISPR for MAP2K4
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for MAP2K4
- GenScript: Design CRISPR guide RNA sequences for MAP2K4
miRNA for MAP2K4 Gene
- miRTarBase miRNAs that target MAP2K4
-
- hsa-mir-375 (MIRT019756)
- hsa-mir-15b-5p (MIRT023483)
- hsa-mir-26b-5p (MIRT028937)
- hsa-mir-25-3p (MIRT030360)
- hsa-mir-145-5p (MIRT053655)
- hsa-mir-188-5p (MIRT053698)
- hsa-mir-27a-3p (MIRT054442)
- hsa-mir-92a-3p (MIRT437965)
- hsa-mir-1185-2-3p (MIRT473851)
- hsa-mir-1185-1-3p (MIRT473852)
- hsa-let-7f-2-3p (MIRT473853)
- hsa-mir-542-3p (MIRT473854)
- hsa-mir-4261 (MIRT473855)
- hsa-mir-3134 (MIRT473856)
- hsa-mir-8082 (MIRT473857)
- hsa-mir-4534 (MIRT473858)
- hsa-mir-4433a-3p (MIRT473859)
- hsa-mir-3688-3p (MIRT473860)
- hsa-mir-6818-3p (MIRT556303)
- hsa-mir-7855-5p (MIRT556304)
- hsa-mir-4325 (MIRT556305)
- hsa-mir-7703 (MIRT556306)
- hsa-mir-6749-3p (MIRT556307)
- hsa-mir-6792-3p (MIRT556308)
- hsa-mir-4691-5p (MIRT556309)
- hsa-mir-3185 (MIRT556310)
- hsa-mir-3121-5p (MIRT556311)
- hsa-mir-660-3p (MIRT556312)
- hsa-mir-5193 (MIRT556313)
- hsa-mir-3614-5p (MIRT556314)
- hsa-mir-10a-3p (MIRT556315)
- hsa-mir-559 (MIRT556316)
- hsa-mir-548y (MIRT556317)
- hsa-mir-548w (MIRT556318)
- hsa-mir-548o-5p (MIRT556319)
- hsa-mir-548j-5p (MIRT556320)
- hsa-mir-548i (MIRT556321)
- hsa-mir-548h-5p (MIRT556322)
- hsa-mir-548d-5p (MIRT556323)
- hsa-mir-548c-5p (MIRT556324)
- hsa-mir-548bb-5p (MIRT556325)
- hsa-mir-548b-5p (MIRT556326)
- hsa-mir-548ay-5p (MIRT556327)
- hsa-mir-548au-5p (MIRT556328)
- hsa-mir-548as-5p (MIRT556329)
- hsa-mir-548ar-5p (MIRT556330)
- hsa-mir-548aq-5p (MIRT556331)
- hsa-mir-548ap-5p (MIRT556332)
- hsa-mir-548am-5p (MIRT556333)
- hsa-mir-548ak (MIRT556334)
- hsa-mir-548ae-5p (MIRT556335)
- hsa-mir-548ad-5p (MIRT556336)
- hsa-mir-548ab (MIRT556337)
- hsa-mir-548a-5p (MIRT556338)
- hsa-mir-363-3p (MIRT556339)
- hsa-mir-367-3p (MIRT556340)
- hsa-mir-92b-3p (MIRT556341)
- hsa-mir-32-5p (MIRT556342)
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for MAP2K4
- Browse OriGene Inhibitory RNA Products For MAP2K4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MAP2K4
- genomics-online: shRNA clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
Clone Products
- Sino Biological Human cDNA Clone for MAP2K4
- Addgene plasmids for MAP2K4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for MAP2K4
- Applied Biological Materials Clones for MAP2K4
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
Cell Line Products
-
Horizon Cell Lines for MAP2K4
No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MAP2K4 Gene
Localization for MAP2K4 Gene
Subcellular locations from UniProtKB/Swiss-Prot for MAP2K4 Gene
- Cytoplasm. Nucleus.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytosol | 5 |
| extracellular | 2 |
| mitochondrion | 2 |
| plasma membrane | 1 |
| cytoskeleton | 1 |
- Cell Junctions (2)
- Nucleoplasm (2)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005634 | nucleus | IEA | -- |
| GO:0005737 | cytoplasm | IBA | -- |
| GO:0005829 | cytosol | TAS,IEA | -- |
| GO:0030424 | axon | IEA | -- |
| GO:0032839 | dendrite cytoplasm | IEA | -- |
Pathways & Interactions for MAP2K4 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Activated TLR4 signalling | ||
| 2 | IL-2 Pathway |
.59
.48
|
.43
.37
.36
.34
|
| 3 | 4-1BB Pathway |
.54
.54
.48
|
.36
.30
|
| 4 | TNFR1 Pathway |
.56
.55
|
.35
|
| 5 | Toll-like Receptor Signaling Pathway | ||
Pathways by source for MAP2K4 Gene
11 Sino Biological pathways for MAP2K4 Gene
2 GeneTex pathways for MAP2K4 Gene
39 BioSystems pathways for MAP2K4 Gene
34 Reactome pathways for MAP2K4 Gene
2 PharmGKB pathways for MAP2K4 Gene
15 KEGG pathways for MAP2K4 Gene
30 GeneGo (Thomson Reuters) pathways for MAP2K4 Gene
3 R&D Systems pathways for MAP2K4 Gene
87 Qiagen pathways for MAP2K4 Gene
1 Cell Signaling Technology pathway for MAP2K4 Gene
Interacting Proteins for MAP2K4 Gene
Selected Interacting proteins:
P45985-MP2K4_HUMAN
ENSP00000262445
for MAP2K4 Gene via
MINT
STRING
UniProtKB
IID
GPS-Prot Interaction Network for MAP2K4
SIGNOR curated interactions for MAP2K4 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000187 | activation of MAPK activity | IBA | -- |
| GO:0001934 | positive regulation of protein phosphorylation | IEA | -- |
| GO:0006468 | protein phosphorylation | IEA | -- |
| GO:0006915 | apoptotic process | IEA | -- |
| GO:0007165 | signal transduction | TAS | 7716521 |
Drugs & Compounds for MAP2K4 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Dabrafenib | Approved, Investigational | Pharma | Inhibition, inhibitor | BRAF kinase inhibitor, Kinase Inhibitors, Mutant BRAF Kinase | 92 | |
| Trametinib | Approved | Pharma | inhibitor, Biomarker | MEK 1/2 inhibitor, MEK Inhibitors, Kinase Inhibitors, Mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors | 151 | |
| ATP | Investigational | Nutra | Agonist, Activator, Full agonist, Antagonist, Potentiation, Pore Blocker | 0 | ||
| PD-0325901 | Investigational | Pharma | inhibitor | 0 | ||
| Selumetinib | Investigational | Pharma | inhibitor, Biomarker | MEK inhibitor, MEK Inhibitors, Kinase Inhibitors, Mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors | 0 |
| Name | Synonyms | Role | CAS Number | PubChem IDs | PubMed IDs | |
|---|---|---|---|---|---|---|
| ADP |
|
Full agonist, Agonist, Partial agonist, Antagonist, Gating inhibitor | 58-64-0 |
|
Drug Products
Transcripts for MAP2K4 Gene
mRNA/cDNA for MAP2K4 Gene
- (6) REFSEQ mRNAs :
- (14) Additional mRNA sequences :
- (191) Selected AceView cDNA sequences:
- (15) Ensembl transcripts including schematic representations, and UCSC links where relevant :
-
- ENST00000353533 3826 bps (uc002gnj.5)
- ENST00000582183 551 bps (uc060bif.1)
- ENST00000415385 3856 bps (uc002gnk.5)
- ENST00000602811 1136 bps (uc060bii.1)
- ENST00000602375 1064 bps (uc060bih.1)
Unigene Clusters for MAP2K4 Gene
- Mitogen-activated protein kinase kinase 4:
- Representative Sequences:
CRISPR Products
-
OriGene CRISPR knockouts for MAP2K4
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for MAP2K4
- genomics-online: gRNA clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- Applied Biological Materials CRISPR for MAP2K4
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for MAP2K4
- GenScript: Design CRISPR guide RNA sequences for MAP2K4
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for MAP2K4
- Browse OriGene Inhibitory RNA Products For MAP2K4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MAP2K4
- genomics-online: shRNA clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
Clone Products
- Sino Biological Human cDNA Clone for MAP2K4
- Addgene plasmids for MAP2K4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for MAP2K4
- Applied Biological Materials Clones for MAP2K4
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | ^ | 7 | ^ | 8a | · | 8b | ^ | 9a | · | 9b | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13a | · | 13b | · | 13c |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | ||||||||||||||||||||||||||||||||
| SP2: | - | - | - | - | |||||||||||||||||||||||||||||||
| SP3: | - | - | |||||||||||||||||||||||||||||||||
| SP4: | - | - | - | - | |||||||||||||||||||||||||||||||
| SP5: | |||||||||||||||||||||||||||||||||||
| SP6: |
Expression for MAP2K4 Gene
mRNA expression in embryonic tissues and stem cells from LifeMap Discovery
- Brain (Nervous System)
- Neural Tube (Nervous System)
-
Blood (Hematopoietic System)
- Granulocytes Peripheral Blood
This gene is overexpressed in Frontal cortex (9.5) and Brain (8.7).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MAP2K4 Gene
- Elite partner
NURSA nuclear receptor signaling pathways regulating expression of MAP2K4 Gene:
MAP2K4SOURCE GeneReport for Unigene cluster for MAP2K4 Gene:
Hs.514681mRNA Expression by UniProt/SwissProt for MAP2K4 Gene:
P45985-MP2K4_HUMAN
Tissue specificity:
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Evidence on tissue expression from TISSUES for MAP2K4 Gene
- Nervous system(5)
- Liver(4.4)
- Kidney(2.1)
Primer Products
-
OriGene qPCR primer pairs for MAP2K4
-
OriGene qPCR primer pairs and template standards for MAP2K4
- genomics-online: primer clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
Gene Expression Assay Products
No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for MAP2K4 Gene
Orthologs for MAP2K4 Gene
This gene was present in the common ancestor of animals and fungi.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| chimpanzee (Pan troglodytes) |
Mammalia | MAP2K4 34 33 |
|
OneToOne | |
| oppossum (Monodelphis domestica) |
Mammalia | -- 34 |
|
OneToMany | |
| -- 34 |
|
OneToMany | |||
| dog (Canis familiaris) |
Mammalia | MAP2K4 34 33 |
|
OneToOne | |
| mouse (Mus musculus) |
Mammalia | Map2k4 16 34 33 |
|
||
| rat (Rattus norvegicus) |
Mammalia | Map2k4 33 |
|
||
| cow (Bos Taurus) |
Mammalia | MAP2K4 34 33 |
|
OneToOne | |
| platypus (Ornithorhynchus anatinus) |
Mammalia | MAP2K4 34 |
|
OneToOne | |
| chicken (Gallus gallus) |
Aves | MAP2K4 34 33 |
|
OneToOne | |
| lizard (Anolis carolinensis) |
Reptilia | MAP2K4 34 |
|
OneToOne | |
| tropical clawed frog (Silurana tropicalis) |
Amphibia | map2k4 33 |
|
||
| African clawed frog (Xenopus laevis) |
Amphibia | Xl.13308 33 |
|
||
| zebrafish (Danio rerio) |
Actinopterygii | map2k4b 34 |
|
OneToMany | |
| map2k4a 34 33 |
|
OneToMany | |||
| Dr.10451 33 |
|
||||
| rainbow trout (Oncorhynchus mykiss) |
Actinopterygii | Omy.4083 33 |
|
||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP003365 33 |
|
||
| fruit fly (Drosophila melanogaster) |
Insecta | Mkk4 34 35 33 |
|
OneToOne | |
| lic 35 |
|
|
|||
| worm (Caenorhabditis elegans) |
Secernentea | sek-1 35 |
|
|
|
| VZC374L.1 35 |
|
|
|||
| mkk-4 34 |
|
OneToMany | |||
| sek-6 34 |
|
OneToMany | |||
| jkk-1 35 |
|
|
|||
| F35C8.1 35 |
|
|
|||
| sek-3 34 |
|
OneToMany | |||
| sea squirt (Ciona savignyi) |
Ascidiacea | -- 34 |
|
OneToOne | |
| fission yeast (Schizosaccharomyces pombe) |
Schizosaccharomycetes | wis1 33 |
|
- Species where no ortholog for MAP2K4 was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Ashbya gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- Alicante grape (Vitis vinifera)
- alpha proteobacteria (Wolbachia pipientis)
- amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- baker's yeast (Saccharomyces cerevisiae)
- barley (Hordeum vulgare)
- beta proteobacteria (Neisseria meningitidis)
- bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- common water flea (Daphnia pulex)
- corn (Zea mays)
- E. coli (Escherichia coli)
- filamentous fungi (Aspergillus nidulans)
- Firmicute bacteria (Streptococcus pneumoniae)
- green algae (Chlamydomonas reinhardtii)
- honey bee (Apis mellifera)
- K. lactis yeast (Kluyveromyces lactis)
- loblloly pine (Pinus taeda)
- malaria parasite (Plasmodium falciparum)
- medicago trunc (Medicago Truncatula)
- moss (Physcomitrella patens)
- orangutan (Pongo pygmaeus)
- pig (Sus scrofa)
- rice (Oryza sativa)
- rice blast fungus (Magnaporthe grisea)
- schistosome parasite (Schistosoma mansoni)
- sea anemone (Nematostella vectensis)
- sea urchin (Strongylocentrotus purpuratus)
- sorghum (Sorghum bicolor)
- soybean (Glycine max)
- stem rust fungus (Puccinia graminis)
- sugarcane (Saccharum officinarum)
- thale cress (Arabidopsis thaliana)
- tomato (Lycopersicon esculentum)
- toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- wheat (Triticum aestivum)
Evolution for MAP2K4 Gene
- ENSEMBL:
- Gene Tree for MAP2K4 (if available)
- TreeFam:
- Gene Tree for MAP2K4 (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for MAP2K4: view image
Paralogs for MAP2K4 Gene
(9) SIMAP similar genes for MAP2K4 Gene using alignment to 5 proteins:
- MP2K4_HUMAN
- J3QLE2_HUMAN
- K7EP58_HUMAN
- R4GN37_HUMAN
- R4GN68_HUMAN
Pseudogenes.org Pseudogenes for MAP2K4 Gene
Variants for MAP2K4 Gene
| SNP ID | Clin | Chr 17 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| rs753665559 | A colorectal adenocarcinoma sample | 12,125,315(+) | G/A | coding_sequence_variant, missense_variant, non_coding_transcript_variant | |
| VAR_040818 | A lung squamous cell carcinoma sample | p.Gln142Leu | |||
| VAR_040819 | A colorectal adenocarcinoma sample | p.Arg154Trp | |||
| VAR_040820 | An ovarian serous carcinoma sample | p.Asn234Ile | |||
| VAR_040821 | A metastatic melanoma sample | p.Ser251Asn |
Residual Variation Intolerance Score:
25.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
0.42;
9.23% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for MAP2K4 Gene
SNP Genotyping and Copy Number Assay Products
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MAP2K4 Gene
Disorders for MAP2K4 Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| cerebral cavernous malformations 2 |
|
|
Search MAP2K4 in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for MAP2K4
- Genetic Association Database
- (GAD)
- Human Genome Epidemiology Navigator
- (HuGE)
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
No data available for UniProtKB/Swiss-Prot and Genatlas for MAP2K4 Gene
Publications for MAP2K4 Gene
- The association between -1304T>G polymorphism in the promoter of MKK4 gene and the risk of sporadic colorectal cancer in southern Chinese population. (PMID: 19610067) Wei Y … Wang J (International journal of cancer 2009) 3 22 44 58
- A docking site in MKK4 mediates high affinity binding to JNK MAPKs and competes with similar docking sites in JNK substrates. (PMID: 12788955) Ho DT … Bardwell L (The Journal of biological chemistry 2003) 3 4 22 58
- Alterations in pancreatic, biliary, and breast carcinomas support MKK4 as a genetically targeted tumor suppressor gene. (PMID: 9622070) Su GH … Kern SE (Cancer research 1998) 3 4 22 58
- Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2. (PMID: 7716521) Lin A … Karin M (Science (New York, N.Y.) 1995) 2 3 4 58
- Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms. (PMID: 7839144) Dérijard B … Davis RJ (Science (New York, N.Y.) 1995) 3 4 22 58
Products for MAP2K4 Gene
- R&D Systems Antibodies for MAP2K4 (MKK4)
- Browse R&D Systems for Human Recombinant Proteins
- Browse R&D Systems for biochemical assays
- Browse Human Proteins and Enzymes
- Browse Primary Antibodies
- Browse Proteins and Enzymes
- Browse ELISAs
- Browse Activity Assays
- Browse cDNA Clones
- Browse Cell Culture Products
- Browse Cell Selection and Detection Kits
- Browse DNA Damage and Repair Kits
- Browse ELISpot/FluoroSpot Kits and Development Modules
- Browse Flow Cytometry Kits
- Browse Immunoprecipitation Assays
- Browse Luminex Assays
- Browse Peptides
- Browse Proteome Profiler Antibody Arrays
- Browse Small Molecules
- Custom Antibody ServicesOriGene Antibodies for MAP2K4
- AM06144SU-N
- AM06580SU-N
- AP00207PU-N
- AP01678PU-N
- AP01679PU-N
- AP02453PU-N
- AP02453PU-S
- AP02454PU-N
- AP02454PU-S
- AP02660PU-N
- AP02660PU-S
- AP02661PU-N
- AP02661PU-S
- AP06311PU-N
- AP06312PU-N
- AP20270PU-N
- AP20418PU-N
- CF500403
- CF500411
- CF500412
- CF500428
- CF500430
- TA300116
- TA300580
- TA300743
- TA309998
- TA309999
- TA321550
- TA325642
- TA325643
- TA326264
- TA327868
- TA327914
- TA333238
- TA333239
- TA500403
- TA500411
- TA500412
- TA500428
- TA500430
- Browse OriGene ELISA Kits
- Custom Assay Services
- OriGene Purified Proteins for MAP2K4
- Search Origene for MassSpec and Protein Over-expression Lysates for MAP2K4
- Origene Custom Protein Services for MAP2K4
- Origene shrna, sirna, and RNAi products in human, mouse, rat for MAP2K4
- Browse OriGene Inhibitory RNA Products For MAP2K4
- OriGene qPCR primer pairs for MAP2K4
- OriGene qPCR primer pairs and template standards for MAP2K4
- OriGene CRISPR knockouts for MAP2K4
- OriGene ORF clones in human for MAP2K4
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For MAP2K4
- GenScript: Next-day shipping of latest version cDNA ORF clones for MAP2K4 in any vector
- GenScript Custom Purified and Recombinant Proteins Services for MAP2K4
- GenScript Custom Assay Services for MAP2K4
- GenScript Custom overexpressing Cell Line Services for MAP2K4
- GenScript: Design CRISPR guide RNA sequences for MAP2K4
- Design optimal peptide antigens
- CloneReady with Over 120,000 Genes
- Gene Synthesis: Any Gene in Any Vector
- Vector-based siRNA and miRNA, Ready for Transfection
- Gene Mutant Library, Variants up to 10^11
- Plasmid Preparation
- GenScript Custom Peptide Services for MAP2K4
- Sino Biological Human cDNA Clone for MAP2K4
- Sino Biological Cell Lysates for MAP2K4
- Sino Biological Recombinant Proteins for MAP2K4
- Sino Biological Antibodies for MAP2K4
- Browse Sino Biological Assays
- Browse Recombinant Proteins
- Browse Antibodies
- Browse cDNA Clones
- Browse ELISA Kits
- Browse CRO Services
- High-throughput Antibody Production Service
- Protein Production Service
- Epitope Tag Antibodies & Reporter Protein Antibodies
- Lentiviral ORF Clones
- Novus Biologicals Antibodies for MAP2K4
- Novus Biologicals lysates and proteins for MAP2K4
- Novus Biologicals
- Novus Biologicals Tissue Microarrays
- Browse Antibodies
- Browse Peptides and Proteins
- Browse Lysates
- Browse ELISA Kits
- Abcam antibodies for MAP2K4
- Abcam proteins for MAP2K4
- Find your target
- Browse Primary Antibodies
- Browse Conjugated Primary Antibodies
- Browse Secondary Antibodies
- Browse ELISA Kits
- Browse Matched Antibody Pairs
- Browse Proteins and Peptides
- Search Knockout (KO) Validated Antibodies
- Browse Monoclonal Antibodies
- Browse Recombinant Antibodies
- Invitrogen Antibodies for MAP2K4 (AFLGC-MAP2K4)
- Cyagen custom Knockout/knockin (KOKI) mouse models for MAP2K4
- Addgene plasmids for MAP2K4
- antibodies-online: Search results for 338 available MAP2K4 Antibodies ranked by validation data
- Compare Top MAP2K4 Antibodies
- Recommended
- 83 WB Antibodies (anti-rat)
- 24 IHC Antibodies (anti-rat)
- 12 IF (p) Antibodies (anti-rat)
- 12 IHC (p) Antibodies (anti-rat)
- 1 IP Antibody (anti-rat)
- 223 WB Antibodies (anti-human)
- 41 IHC Antibodies (anti-human)
- 16 IHC (p) Antibodies (anti-human)
- 12 IF (p) Antibodies (anti-human)
- 4 IP Antibodies (anti-human)
- 116 WB Antibodies (anti-mouse)
- 26 IHC Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 12 IHC (p) Antibodies (anti-mouse)
- 1 IP Antibody (anti-mouse)
- antibodies-online: Search results for 8 available MAP2K4 Elisa Kits ranked by validation data
- Compare Top MAP2K4 Elisa Kits
- antibodies-online: Search results for 11 available MAP2K4 Proteins ranked by validation data
- Compare Top MAP2K4 Proteins
- GeneTex MAP2K4 Antibody for MAP2K4
- Search GeneTex for Proteins for MAP2K4
- Santa Cruz Biotechnology (SCBT) Antibodies for MAP2K4
- Search Santa Cruz Biotechnology (SCBT) for MAP2K4 siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for MAP2K4
- Horizon Cell Lines for MAP2K4
- genomics-online: cdna clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- orf clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- genomics-online: gRNA clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- genomics-online: primer clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- genomics-online: shRNA clones - Search results for 111 available MAP2K4 gene related products
- Overview of 111 available MAP2K4 gene related products
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for MAP2K4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for MAP2K4
- GeneCopoeia miRNA 3'UTR Target clone products for MAP2K4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for MAP2K4
Sources for MAP2K4 Gene
- (1) GeneCards
- (2) HGNC
- (3) EntrezGene
- (4) Swiss-Prot
- (5) Ensembl
- (6) OMIM
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) GO
- (12) TrEMBL
- (13) InterPro
- (14) ProtoNet
- (15) Blocks
- (16) MGI
- (17) IUBMB
- (18) KEGG
- (19) MINT
- (20) STRING
- (21) IntAct
- (22) Novoseek
- (23) PharmGKB
- (24) DrugBank
- (25) HMDB
- (26) UniGene
- (27) AceView
- (28) ASD
- (29) ECgene
- (30) GeneAnnot
- (31) CGAP SAGE
- (32) SOURCE
- (33) HomoloGene
- (34) PanEnsembl
- (35) euGenes
- (36) SGD
- (37) FlyBase
- (38) WormBase
- (39) Pseudogene
- (40) DGV
- (41) dbSNP
- (42) GenAtlas
- (43) HGMD
- (44) GAD
- (45) BGMUT
- (46) HuGE
- (47) Atlas
- (48) Cell Signaling Technology
- (49) GenBank
- (50) H-invDB
- (51) HORDE
- (52) HUGE
- (53) IMGT
- (54) Leiden
- (55) miRBase
- (56) DME
- (57) OriGene
- (58) PubMed
- (59) R&D Systems
- (60) TGDB
- (61) Tocris
- (62) Abcam
- (63) Novus Biologicals
- (64) ProSpec
- (65) Sino Biological
- (66) GenScript
- (67) Qiagen
- (68) Cloud-Clone Corp.
- (69) OCA
- (70) Proteopedia
- (71) MOPED
- (72) neXtProt
- (73) Reactome
- (74) GeneGo (Thomson Reuters)
- (75) fRNAdb
- (76) DISEASES
- (77) SIMAP
- (78) GenomeRNAi
- (79) LifeMap
- (80) miRTarBase
- (81) MalaCards
- (82) Invitrogen
- (83) BitterDB
- (84) Vector BioLabs
- (85) ESI-BIO
- (86) RefSeq
- (87) BioSystems
- (88) MaxQB
- (89) IUPHAR
- (90) BioGPS
- (91) Illumina
- (92) COMPARTMENTS
- (93) HOMER
- (94) PaxDb
- (95) ApexBio
- (96) Addgene
- (97) antibodies-online
- (98) CYP
- (99) NONCODE
- (100) SwitchGear Genomics
- (101) TreeFam
- (102) PathCards
- (103) GeneReviews
- (104) GeneTex
- (105) Taconic Biosciences
- (106) GTEx
- (107) ProteomicsDB
- (108) SCBT
- (109) DGIdb
- (110) ClinicalTrials
- (111) FDA Approved Drugs
- (112) RVIS
- (113) SIGNOR
- (114) diseasecard
- (115) NIH Rare Diseases
- (116) Orphanet
- (117) UMLS
- (118) GTR
- (119) Disease Ontology
- (120) Genetics Home Reference
- (121) MeSH
- (122) MedlinePlus
- (123) CDC
- (124) NINDS
- (125) NCBI Bookshelf
- (126) ClinVar
- (127) Gene Damage Index
- (128) ViGene Biosciences
- (129) HPO
- (130) UDN
- (131) VISTA
- (132) FANTOM5
- (133) ENCODE
- (134) ProSci
- (135) Horizon
- (136) NURSA
- (137) IID
- (138) Cyagen
- (139) VectorBuilder
- (140) SNPedia
- (141) BRCA Exchange
- (142) St John's Lab
- (143) CIViC
- (144) ProteoGenix
- (145) dbSUPER
- (146) TISSUES
- (147) Gene ORGANizer
- (148) abm
- (149) CrownBio
- (150) Human Protein Atlas
- (151) GWAS Catalog
- (152) Monarch Initiative
- (153) DataMed
- (154) HumanCyc
- (155) genomics-online
- (156) UCNEbase
- (157) EPDnew
- (158) Applied Biosystems
- (159) GlyConnect
- (160) Aminode
- (161) GeneCopoeia
- (162) GPS-Prot
- (163) RNACentral
- (164) LncRNADisease
- (165) LOVD
- (166) miR2Disease
- (167) DiseaseEnhancer
- (168) Boster Bio
- (169) Biorbyt