This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during met... See more...

Aliases for KLF8 Gene

Aliases for KLF8 Gene

  • Kruppel Like Factor 8 2 3 5
  • ZNF741 2 3 4
  • BKLF3 2 3 4
  • Basic Krueppel-Like Factor 3 3 4
  • Zinc Finger Protein 741 3 4
  • Krueppel-Like Factor 8 3 4
  • Basic Kruppel-Like Factor 3 3
  • DXS741 2
  • KLF8 5

External Ids for KLF8 Gene

Previous GeneCards Identifiers for KLF8 Gene

  • GC0XP053239
  • GC0XP054197
  • GC0XP054864
  • GC0XP055226
  • GC0XP056141
  • GC0XP056275

Summaries for KLF8 Gene

Entrez Gene Summary for KLF8 Gene

  • This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

GeneCards Summary for KLF8 Gene

KLF8 (Kruppel Like Factor 8) is a Protein Coding gene. Among its related pathways are Signaling events mediated by focal adhesion kinase. An important paralog of this gene is KLF12.

UniProtKB/Swiss-Prot Summary for KLF8 Gene

  • Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).

Gene Wiki entry for KLF8 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for KLF8 Gene

Genomics for KLF8 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for KLF8 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH0XJ055907 Promoter/Enhancer 1.7 EPDnew Ensembl ENCODE CraniofacialAtlas 600.7 +0.7 678 3.2 CTCF PKNOX1 RAD21 IKZF2 YY1 EED RFX1 EGR1 BCLAF1 GABPA ENSG00000227486 KLF8 NBDY UBQLN2 GOT2P6
GH0XJ056231 Promoter/Enhancer 1.6 EPDnew Ensembl ENCODE CraniofacialAtlas 600.1 +325.1 325078 3.6 POLR2A PKNOX1 CTCF MAZ SIN3A VEZF1 KLF1 TAF1 EGR1 EZH2 KLF8 SPIN2B lnc-SPIN3-6 piR-53063-269 UBQLN2
GH0XJ056107 Promoter 0.6 EPDnew 600.1 +199.3 199277 0.1 MLLT1 ZBTB17 SIN3A ELF1 MNT POLR2A KLF8 piR-61532-349 piR-52368-177 ENSG00000227486 UBQLN2
GH0XJ055716 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 10.6 -190.0 -189968 2.5 CEBPG SP1 CREB1 CTCF PRDM10 TFE3 RFX1 POLR2A FOS LARP7 RRAGB ENSG00000227486 KLF8 MAGEH1 piR-44878-075
GH0XJ055488 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE CraniofacialAtlas 3.6 -418.8 -418796 4.1 POLR2A MTA1 PATZ1 CTCF ZNF561 SCRT2 NRF1 SP2 EGR1 ZXDB LOC101928692 USP51 lnc-MAGEH1-1 lnc-USP51-1 MAGEH1 KLF8
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around KLF8 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for KLF8

Top Transcription factor binding sites by QIAGEN in the KLF8 gene promoter:
  • aMEF-2
  • AML1a
  • c-Myb
  • LyF-1
  • MEF-2A
  • Nkx2-5
  • POU2F1
  • POU2F1a
  • ZID

Genomic Locations for KLF8 Gene

Genomic Locations for KLF8 Gene
chrX:55,908,123-56,291,531
(GRCh38/hg38)
Size:
383,409 bases
Orientation:
Plus strand
chrX:56,258,822-56,314,322
(GRCh37/hg19)
Size:
55,501 bases
Orientation:
Plus strand

Genomic View for KLF8 Gene

Genes around KLF8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
KLF8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for KLF8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for KLF8 Gene

Proteins for KLF8 Gene

  • Protein details for KLF8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O95600-KLF8_HUMAN
    Recommended name:
    Krueppel-like factor 8
    Protein Accession:
    O95600
    Secondary Accessions:
    • B4DJN3
    • E7EQQ8
    • L0R3U8
    • L0R4U2
    • Q2M246
    • Q59GV5
    • Q5HYQ5
    • Q5JXP7
    • Q6MZJ7
    • Q9UGC4

    Protein attributes for KLF8 Gene

    Size:
    359 amino acids
    Molecular mass:
    39314 Da
    Quaternary structure:
    • Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.

    Alternative splice isoforms for KLF8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for KLF8 Gene

Post-translational modifications for KLF8 Gene

  • Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Abcam antibodies for KLF8
  • Santa Cruz Biotechnology (SCBT) Antibodies for KLF8

No data available for DME Specific Peptides for KLF8 Gene

Domains & Families for KLF8 Gene

Gene Families for KLF8 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins
  • Transcription factors

Protein Domains for KLF8 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for KLF8 Gene

GenScript: Design optimal peptide antigens:
  • Zinc finger protein 741 (KLF8_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O95600

UniProtKB/Swiss-Prot:

KLF8_HUMAN :
  • The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF8, the motif is inactive.
  • Belongs to the Sp1 C2H2-type zinc-finger protein family.
Domain:
  • The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF8, the motif is inactive.
Family:
  • Belongs to the Sp1 C2H2-type zinc-finger protein family.
genes like me logo Genes that share domains with KLF8: view

Function for KLF8 Gene

Molecular function for KLF8 Gene

UniProtKB/Swiss-Prot Function:
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).

Phenotypes From GWAS Catalog for KLF8 Gene

Gene Ontology (GO) - Molecular Function for KLF8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000976 transcription regulatory region sequence-specific DNA binding IBA 21873635
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IBA --
GO:0000978 RNA polymerase II proximal promoter sequence-specific DNA binding IDA 10756197
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific ISA --
GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific IMP 10756197
genes like me logo Genes that share ontologies with KLF8: view
genes like me logo Genes that share phenotypes with KLF8: view

Animal Model Products

CRISPR Products

miRNA for KLF8 Gene

miRTarBase miRNAs that target KLF8

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for KLF8

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for KLF8 Gene

Localization for KLF8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for KLF8 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for KLF8 gene
Compartment Confidence
nucleus 5
cytosol 4
plasma membrane 2
extracellular 2
cytoskeleton 2
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for KLF8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin ISA --
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm IBA,IDA --
GO:0005829 cytosol IDA --
GO:0016235 aggresome IDA --
genes like me logo Genes that share ontologies with KLF8: view

Pathways & Interactions for KLF8 Gene

genes like me logo Genes that share pathways with KLF8: view

Pathways by source for KLF8 Gene

1 BioSystems pathway for KLF8 Gene

SIGNOR curated interactions for KLF8 Gene

Activates:

Gene Ontology (GO) - Biological Process for KLF8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II IMP 10756197
GO:0006357 regulation of transcription by RNA polymerase II IBA --
GO:0045944 positive regulation of transcription by RNA polymerase II IBA 21873635
genes like me logo Genes that share ontologies with KLF8: view

Drugs & Compounds for KLF8 Gene

(1) Drugs for KLF8 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with KLF8: view

Transcripts for KLF8 Gene

mRNA/cDNA for KLF8 Gene

7 REFSEQ mRNAs :
13 NCBI additional mRNA sequence :
6 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for KLF8

Alternative Splicing Database (ASD) splice patterns (SP) for KLF8 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8
SP1: -
SP2: -
SP3:
SP4:

Relevant External Links for KLF8 Gene

GeneLoc Exon Structure for
KLF8

Expression for KLF8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for KLF8 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for KLF8 Gene

This gene is overexpressed in Kidney (67.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for KLF8 Gene



Protein tissue co-expression partners for KLF8 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for KLF8

SOURCE GeneReport for Unigene cluster for KLF8 Gene:

Hs.646614

mRNA Expression by UniProt/SwissProt for KLF8 Gene:

O95600-KLF8_HUMAN
Tissue specificity: Ubiquitous.

Evidence on tissue expression from TISSUES for KLF8 Gene

  • Nervous system(4.4)
  • Skin(2.5)
  • Kidney(2)
  • Blood(2)
  • Heart(2)
genes like me logo Genes that share expression patterns with KLF8: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for KLF8 Gene

Orthologs for KLF8 Gene

This gene was present in the common ancestor of animals.

Orthologs for KLF8 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia KLF8 30 31
  • 100 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia KLF8 30 31
  • 90.37 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia KLF8 30 31
  • 88.3 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Klf8 30
  • 86.01 (n)
Mouse
(Mus musculus)
Mammalia Klf8 30 17 31
  • 85.54 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia KLF8 31
  • 69 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia KLF8 31
  • 55 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia KLF8 31
  • 58 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia klf8 30
  • 64.64 (n)
Str.19926 30
African clawed frog
(Xenopus laevis)
Amphibia ap-2rep 30
Zebrafish
(Danio rerio)
Actinopterygii si:ch211-208m1.2 30
  • 62.24 (n)
KLF8 31
  • 48 (a)
OneToOne
klf12 30
Fruit Fly
(Drosophila melanogaster)
Insecta CG12029 32
  • 75 (a)
CG9895 32
  • 74 (a)
CG42741 31
  • 33 (a)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea F56F11.3 32
  • 72 (a)
F53F8.1 32
  • 70 (a)
mua-1 32
  • 67 (a)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 27 (a)
OneToMany
Species where no ortholog for KLF8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for KLF8 Gene

ENSEMBL:
Gene Tree for KLF8 (if available)
TreeFam:
Gene Tree for KLF8 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for KLF8: view image

Paralogs for KLF8 Gene

(18) SIMAP similar genes for KLF8 Gene using alignment to 2 proteins:

  • KLF8_HUMAN
  • Q05BZ3_HUMAN

Pseudogenes.org Pseudogenes for KLF8 Gene

genes like me logo Genes that share paralogs with KLF8: view

Variants for KLF8 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for KLF8 Gene

SNP ID Clinical significance and condition Chr 0X pos Variation AA Info Type
710494 Benign: not provided 56,265,749(+) G/A NON_CODING_TRANSCRIPT_VARIANT,INTRON_VARIANT
734488 Likely Benign: not provided 56,269,388(+) C/T NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT,INTRON_VARIANT
737681 Likely Benign: not provided 56,265,530(+) C/T NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT
786706 Benign: not provided 56,265,323(+) G/T MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT
rs146429909 Benign: not specified; not provided 56,265,420(+) A/G MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT

Additional dbSNP identifiers (rs#s) for KLF8 Gene

Variation tolerance for KLF8 Gene

Residual Variation Intolerance Score: 69.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.42; 27.84% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for KLF8 Gene

Human Gene Mutation Database (HGMD)
KLF8
SNPedia medical, phenotypic, and genealogical associations of SNPs for
KLF8

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for KLF8 Gene

Disorders for KLF8 Gene

Additional Disease Information for KLF8

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform

No disorders were found for KLF8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for KLF8 Gene

Publications for KLF8 Gene

  1. Human Krüppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription. (PMID: 10756197) van Vliet J … Crossley M (Nucleic acids research 2000) 3 4 23
  2. Shaking the family tree: identification of novel and biologically active alternatively spliced isoforms across the KLF family of transcription factors. (PMID: 23134681) Camacho-Vanegas O … Martignetti JA (FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013) 3 4
  3. KLF8 transcription factor participates in oncogenic transformation. (PMID: 16832343) Wang X … Zhao J (Oncogene 2007) 3 23
  4. Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation. (PMID: 16617055) Wei H … Zhao J (The Journal of biological chemistry 2006) 3 4
  5. The DNA sequence of the human X chromosome. (PMID: 15772651) Ross MT … Bentley DR (Nature 2005) 3 4

Products for KLF8 Gene

Sources for KLF8 Gene