The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has... See more...

Aliases for ILF2 Gene

Aliases for ILF2 Gene

  • Interleukin Enhancer Binding Factor 2 2 3 5
  • NF45 2 3 4
  • Interleukin Enhancer Binding Factor 2, 45kDa 2 3
  • Interleukin Enhancer Binding Factor 2, 45kD 2 3
  • Interleukin Enhancer-Binding Factor 2 3 4
  • Nuclear Factor Of Activated T-Cells, 45-KDa 3
  • Nuclear Factor Of Activated T-Cells 45 KDa 4
  • PRO3063 3
  • ILF2 5

External Ids for ILF2 Gene

Previous GeneCards Identifiers for ILF2 Gene

  • GC01M151483
  • GC01M149365
  • GC01M150409
  • GC01M150851
  • GC01M150447
  • GC01M151900
  • GC01M153634
  • GC01M124997

Summaries for ILF2 Gene

Entrez Gene Summary for ILF2 Gene

  • The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]

GeneCards Summary for ILF2 Gene

ILF2 (Interleukin Enhancer Binding Factor 2) is a Protein Coding gene. Diseases associated with ILF2 include Childhood Endodermal Sinus Tumor. Among its related pathways are B cell receptor signaling pathway (KEGG) and Innate Immune System. Gene Ontology (GO) annotations related to this gene include double-stranded RNA binding. An important paralog of this gene is ZFR2.

UniProtKB/Swiss-Prot Summary for ILF2 Gene

  • Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.

Gene Wiki entry for ILF2 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ILF2 Gene

Genomics for ILF2 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ILF2 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ILF2 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ILF2

Top Transcription factor binding sites by QIAGEN in the ILF2 gene promoter:
  • E2F
  • E2F-1
  • E2F-2
  • E2F-3a
  • E2F-4
  • E2F-5
  • GATA-1
  • NF-1/L
  • ZID

Genomic Locations for ILF2 Gene

Genomic Locations for ILF2 Gene
chr1:153,661,788-153,671,028
(GRCh38/hg38)
Size:
9,241 bases
Orientation:
Minus strand
chr1:153,634,512-153,643,524
(GRCh37/hg19)
Size:
9,013 bases
Orientation:
Minus strand

Genomic View for ILF2 Gene

Genes around ILF2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ILF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ILF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ILF2 Gene

Proteins for ILF2 Gene

  • Protein details for ILF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q12905-ILF2_HUMAN
    Recommended name:
    Interleukin enhancer-binding factor 2
    Protein Accession:
    Q12905
    Secondary Accessions:
    • A6NDB0
    • B2R8G7
    • Q5SR10
    • Q5SR11
    • Q7L7R3
    • Q9BWD4
    • Q9P1N0

    Protein attributes for ILF2 Gene

    Size:
    390 amino acids
    Molecular mass:
    43062 Da
    Quaternary structure:
    • Forms heterodimers with ILF3. ILF2-ILF3 heterodimers may also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of G22P1/KU70 and XRCC5/KU80. Forms a complex with ILF3, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with IGF2BP1.
    SequenceCaution:
    • Sequence=AAA20993.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=AAF29591.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA.; Evidence={ECO:0000305};

neXtProt entry for ILF2 Gene

Post-translational modifications for ILF2 Gene

  • Ubiquitination at Lys45, Lys59, and Lys141
  • Modification sites at PhosphoSitePlus

Other Protein References for ILF2 Gene

No data available for DME Specific Peptides for ILF2 Gene

Domains & Families for ILF2 Gene

Gene Families for ILF2 Gene

Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for ILF2 Gene

Suggested Antigen Peptide Sequences for ILF2 Gene

GenScript: Design optimal peptide antigens:
  • Nuclear factor of activated T-cells 45 kDa (ILF2_HUMAN)
genes like me logo Genes that share domains with ILF2: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for ILF2 Gene

Function for ILF2 Gene

Molecular function for ILF2 Gene

UniProtKB/Swiss-Prot Function:
Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.

Phenotypes From GWAS Catalog for ILF2 Gene

Gene Ontology (GO) - Molecular Function for ILF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA,IDA 7519613
GO:0003723 RNA binding IEA,HDA 22658674
GO:0003725 double-stranded RNA binding IDA 10574923
GO:0005515 protein binding IPI 9442054
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with ILF2: view
genes like me logo Genes that share phenotypes with ILF2: view

Animal Models for ILF2 Gene

MGI Knock Outs for ILF2:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ILF2

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ILF2 Gene

Localization for ILF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ILF2 Gene

Nucleus, nucleolus. Cytoplasm. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ILF2 gene
Compartment Confidence
nucleus 5
extracellular 4
cytosol 3
mitochondrion 2
endoplasmic reticulum 2
plasma membrane 1
cytoskeleton 1
endosome 1
lysosome 1
golgi apparatus 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for ILF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region HDA,TAS --
GO:0005634 nucleus IEA,IDA 21123651
GO:0005654 nucleoplasm IDA --
GO:0005730 nucleolus IEA,IDA 11790298
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with ILF2: view

Pathways & Interactions for ILF2 Gene

genes like me logo Genes that share pathways with ILF2: view

Pathways by source for ILF2 Gene

1 BioSystems pathway for ILF2 Gene
3 Reactome pathways for ILF2 Gene

Gene Ontology (GO) - Biological Process for ILF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IDA 11739746
GO:0006955 immune response IEA --
GO:0043312 neutrophil degranulation TAS --
GO:0045893 positive regulation of transcription, DNA-templated IDA 11739746
genes like me logo Genes that share ontologies with ILF2: view

No data available for SIGNOR curated interactions for ILF2 Gene

Drugs & Compounds for ILF2 Gene

(2) Drugs for ILF2 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(1) Additional Compounds for ILF2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with ILF2: view

Transcripts for ILF2 Gene

mRNA/cDNA for ILF2 Gene

2 REFSEQ mRNAs :
11 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ILF2

Alternative Splicing Database (ASD) splice patterns (SP) for ILF2 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
SP1: - - - - - - - -
SP2: - - - - -
SP3: - - - - - - - -
SP4: - -
SP5: - - -
SP6: - -
SP7: -
SP8:
SP9:

Relevant External Links for ILF2 Gene

GeneLoc Exon Structure for
ILF2

Expression for ILF2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ILF2 Gene

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ILF2 Gene



Protein tissue co-expression partners for ILF2 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ILF2

SOURCE GeneReport for Unigene cluster for ILF2 Gene:

Hs.75117

Evidence on tissue expression from TISSUES for ILF2 Gene

  • Lung(4.8)
  • Nervous system(4.8)
  • Liver(4.7)
  • Intestine(3.8)
  • Skin(3.8)
  • Blood(3.6)
  • Eye(2.9)
  • Kidney(2.8)
  • Muscle(2.4)
  • Lymph node(2.4)
  • Bone marrow(2.4)
  • Heart(2.4)
  • Spleen(2.4)
  • Thyroid gland(2.4)
  • Pancreas(2.2)
  • Bone(2.1)
  • Stomach(2.1)
genes like me logo Genes that share expression patterns with ILF2: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ILF2 Gene

Orthologs for ILF2 Gene

This gene was present in the common ancestor of animals.

Orthologs for ILF2 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ILF2 30 31
  • 99.91 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia ILF2 31
  • 98 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia ILF2 30 31
  • 94.02 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia ILF2 30 31
  • 93.25 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Ilf2 30 17 31
  • 91.54 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia ILF2 31
  • 69 (a)
OneToOne
Chicken
(Gallus gallus)
Aves ILF2 30 31
  • 80.52 (n)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia ilf2-prov 30
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia MGC75701 30
Zebrafish
(Danio rerio)
Actinopterygii ilf2 30 31
  • 77.71 (n)
OneToOne
wufb34h01 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1694 30
Fruit Fly
(Drosophila melanogaster)
Insecta CG5641 30 31 32
  • 60.83 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP001997 30
  • 58.81 (n)
Worm
(Caenorhabditis elegans)
Secernentea CELE_R11H6.5 30
  • 45.9 (n)
R11H6.5 31 32
  • 36 (a)
OneToOne
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.5584 31
  • 58 (a)
OneToOne
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.3994 30
Species where no ortholog for ILF2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rat (Rattus norvegicus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for ILF2 Gene

ENSEMBL:
Gene Tree for ILF2 (if available)
TreeFam:
Gene Tree for ILF2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ILF2: view image

Paralogs for ILF2 Gene

Pseudogenes.org Pseudogenes for ILF2 Gene

genes like me logo Genes that share paralogs with ILF2: view

Variants for ILF2 Gene

Additional dbSNP identifiers (rs#s) for ILF2 Gene

Structural Variations from Database of Genomic Variants (DGV) for ILF2 Gene

Variant ID Type Subtype PubMed ID
dgv582n54 CNV gain 21841781
nsv1122407 CNV deletion 24896259
nsv464028 CNV gain 19166990
nsv476355 CNV novel sequence insertion 20440878

Variation tolerance for ILF2 Gene

Residual Variation Intolerance Score: 21.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.18; 3.98% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ILF2 Gene

Human Gene Mutation Database (HGMD)
ILF2
SNPedia medical, phenotypic, and genealogical associations of SNPs for
ILF2

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ILF2 Gene

Disorders for ILF2 Gene

MalaCards: The human disease database

(1) MalaCards diseases for ILF2 Gene - From: DISEASES

Disorder Aliases PubMed IDs
childhood endodermal sinus tumor
  • childhood endodermal sinus neoplasm
- elite association - COSMIC cancer census association via MalaCards
Search ILF2 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ILF2

genes like me logo Genes that share disorders with ILF2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ILF2 Gene

Publications for ILF2 Gene

  1. Cloning and expression of cyclosporin A- and FK506-sensitive nuclear factor of activated T-cells: NF45 and NF90. (PMID: 7519613) Kao PN … Corthésy B (The Journal of biological chemistry 1994) 2 3 4 23
  2. DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45. (PMID: 9442054) Ting NS … Lees-Miller SP (The Journal of biological chemistry 1998) 3 4 23
  3. Phosphorylation of the NFAR proteins by the dsRNA-dependent protein kinase PKR constitutes a novel mechanism of translational regulation and cellular defense. (PMID: 21123651) Harashima A … Barber GN (Genes & development 2010) 3 4
  4. NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response. (PMID: 19893574) Graber TE … Holcik M (Cell death and differentiation 2010) 3 23
  5. NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells. (PMID: 20051514) Kiesler P … Vercelli D (The Journal of biological chemistry 2010) 3 23

Products for ILF2 Gene

  • Signalway ELISA kits for ILF2
  • Signalway Proteins for ILF2

Sources for ILF2 Gene