HTRA3 Gene (Protein Coding)

HtrA Serine Peptidase 3

GC04P008269
GIFtS:
39
See more...

Aliases for HTRA3 Gene

Aliases for HTRA3 Gene

  • HtrA Serine Peptidase 3 2 3 5
  • Pregnancy-Related Serine Protease 2 3 4
  • High-Temperature Requirement Factor A3 3 4
  • Serine Protease HTRA3 3 4
  • Prsp 2 3
  • Tasp 2 3
  • Probable Serine Protease HTRA3 3
  • EC 3.4.21.108 51
  • EC 3.4.21.- 4
  • EC 3.4.21 51
  • HTRA3 5
  • PRSP 4

External Ids for HTRA3 Gene

Previous GeneCards Identifiers for HTRA3 Gene

  • GC04P008238
  • GC04P008421
  • GC04P008464
  • GC04P008389
  • GC04P008271
  • GC04P008191

Summaries for HTRA3 Gene

GeneCards Summary for HTRA3 Gene

HTRA3 (HtrA Serine Peptidase 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include serine-type endopeptidase activity and endopeptidase activity. An important paralog of this gene is HTRA1.

UniProtKB/Swiss-Prot Summary for HTRA3 Gene

  • Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724).

Additional gene information for HTRA3 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for HTRA3 Gene

Genomics for HTRA3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Download GeneHancer 2017 data

GeneHancers around HTRA3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for HTRA3

Top Transcription factor binding sites by QIAGEN in the HTRA3 gene promoter:
  • AhR
  • ATF-2
  • Bach2
  • c-Jun
  • c-Myc
  • GR
  • GR-alpha
  • GR-beta
  • NF-kappaB1
  • Zic3

Genomic Locations for HTRA3 Gene

Latest Assembly
chr4:8,269,712-8,307,098
(GRCh38/hg38)
Size:
37,387 bases
Orientation:
Plus strand

Previous Assembly
chr4:8,271,481-8,308,825
(GRCh37/hg19 by Entrez Gene)
Size:
37,345 bases
Orientation:
Plus strand

chr4:8,271,492-8,308,838
(GRCh37/hg19 by Ensembl)
Size:
37,347 bases
Orientation:
Plus strand

Genomic View for HTRA3 Gene

Genes around HTRA3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
HTRA3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for HTRA3 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for HTRA3 Gene

Proteins for HTRA3 Gene

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  • Protein details for HTRA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P83110-HTRA3_HUMAN
    Recommended name:
    Serine protease HTRA3
    Protein Accession:
    P83110
    Secondary Accessions:
    • Q7Z7A2

    Protein attributes for HTRA3 Gene

    Size:
    453 amino acids
    Molecular mass:
    48608 Da
    Quaternary structure:
    • Homotrimer (PubMed:26110759). Interacts with TGFB1; the interaction inhibits TGFB-mediated signaling. Interacts with BMP4; the interaction inhibits BMP4-mediated signaling. Interacts with TGFB2 and GDF5 (By similarity). Interacts with MYH9.

    Three dimensional structures from OCA and Proteopedia for HTRA3 Gene

    Alternative splice isoforms for HTRA3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for HTRA3 Gene

Protein Expression for HTRA3 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for HTRA3 Gene

P83110:
  • GEVIGINT
  • GNSGGPL
  • NFIADVVEK
  • GSPFALQNT

Post-translational modifications for HTRA3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for HTRA3 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for HTRA3 Gene

Gene Families for HTRA3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted secreted proteins

Protein Domains for HTRA3 Gene

InterPro:
Blocks:
  • PDZ/DHR/GLGF domain
  • Protease inhibitor, Kazal-type
  • HtrA/DegQ protease family signature
ProtoNet:

Suggested Antigen Peptide Sequences for HTRA3 Gene

GenScript: Design optimal peptide antigens:
  • Pregnancy-related serine protease (HTRA3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P83110

UniProtKB/Swiss-Prot:

HTRA3_HUMAN :
  • Belongs to the peptidase S1C family.
Family:
  • Belongs to the peptidase S1C family.
genes like me logo Genes that share domains with HTRA3: view

Function for HTRA3 Gene

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  1. Animal Models for research
  2. CRISPR products for research
  3. miRNA products for research
  4. Inhibitory RNAs for research
  5. Clone products for research
  6. Cell Lines for research

Molecular function for HTRA3 Gene

UniProtKB/Swiss-Prot Function:
Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724).
UniProtKB/Swiss-Prot Induction:
Down-regulated in ovarian and endometrial cancers (EC). Decrease of 3.2-fold in endometrial cancer.

Enzyme Numbers (IUBMB) for HTRA3 Gene

Phenotypes From GWAS Catalog for HTRA3 Gene

Gene Ontology (GO) - Molecular Function for HTRA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004175 endopeptidase activity IDA 18387192
GO:0004252 serine-type endopeptidase activity IEA --
GO:0005515 protein binding IPI 22229724
GO:0005520 insulin-like growth factor binding IEA --
GO:0008233 peptidase activity IEA --
genes like me logo Genes that share ontologies with HTRA3: view
genes like me logo Genes that share phenotypes with HTRA3: view

Animal Models for research

  • Taconic Biosciences Mouse Models for HTRA3

miRNA for HTRA3 Gene

miRTarBase miRNAs that target HTRA3

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for HTRA3

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for HTRA3 Gene

Localization for HTRA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for HTRA3 Gene

Secreted. Note=Secretion increased during decidualization of endometrial stromal cells.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for HTRA3 gene
Compartment Confidence
extracellular 4
plasma membrane 2
nucleus 2
mitochondrion 1
cytosol 1
endoplasmic reticulum 0
lysosome 0
golgi apparatus 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for HTRA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region IEA --
genes like me logo Genes that share ontologies with HTRA3: view

Pathways & Interactions for HTRA3 Gene

PathCards logo

SuperPathways for HTRA3 Gene

No Data Available

Gene Ontology (GO) - Biological Process for HTRA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IDA 18387192
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway ISS --
GO:0030514 negative regulation of BMP signaling pathway ISS --
genes like me logo Genes that share ontologies with HTRA3: view

No data available for Pathways by source and SIGNOR curated interactions for HTRA3 Gene

Drugs & Compounds for HTRA3 Gene

Products:

  1. Drug products for research

(1) Drugs for HTRA3 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with HTRA3: view

Transcripts for HTRA3 Gene

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  3. Inhibitory RNAs for research
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mRNA/cDNA for HTRA3 Gene

2 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for HTRA3

Alternative Splicing Database (ASD) splice patterns (SP) for HTRA3 Gene

No ASD Table

Relevant External Links for HTRA3 Gene

GeneLoc Exon Structure for
HTRA3

Expression for HTRA3 Gene

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  1. Primer products for research
  2. Gene Expression Assays for research

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for HTRA3 Gene

mRNA expression in normal human tissues for HTRA3 Gene
mRNA expression by GTEx for HTRA3 GenemRNA expression by BioGPS for HTRA3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for HTRA3 Gene

This gene is overexpressed in Heart - Atrial Appendage (x7.8).

Protein differential expression in normal tissues from HIPED for HTRA3 Gene

This gene is overexpressed in Bone marrow mesenchymal stem cell (32.5), Heart (25.8), and Peripheral blood mononuclear cells (10.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for HTRA3 Gene



Protein tissue co-expression partners for HTRA3 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for HTRA3

SOURCE GeneReport for Unigene cluster for HTRA3 Gene:

Hs.479119

mRNA Expression by UniProt/SwissProt for HTRA3 Gene:

P83110-HTRA3_HUMAN
Tissue specificity: Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. In the endometrium, expressed in epithelial glands and the stroma. Also present in leukocytes. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.

Evidence on tissue expression from TISSUES for HTRA3 Gene

  • Heart(4.4)
  • Nervous system(4.1)
genes like me logo Genes that share expression patterns with HTRA3: view

Primer products for research

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for HTRA3 Gene

Orthologs for HTRA3 Gene

This gene was present in the common ancestor of animals.

Orthologs for HTRA3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
 Mammalia HTRA3 30 31
  • 97.87 (n)
 OneToOne
Dog
(Canis familiaris)
 Mammalia HTRA3 30 31
  • 88.83 (n)
 OneToOne
Mouse
(Mus musculus)
 Mammalia Htra3 30 17 31
  • 87.66 (n)
 OneToOne
Rat
(Rattus norvegicus)
 Mammalia Htra3 30
  • 86.36 (n)
Cow
(Bos Taurus)
 Mammalia HTRA3 30 31
  • 82.66 (n)
 OneToOne
Oppossum
(Monodelphis domestica)
 Mammalia HTRA3 31
  • 78 (a)
 OneToOne
Platypus
(Ornithorhynchus anatinus)
 Mammalia HTRA3 31
  • 72 (a)
 OneToOne
Chicken
(Gallus gallus)
 Aves HTRA3 30 31
  • 73.63 (n)
 OneToOne
Lizard
(Anolis carolinensis)
 Reptilia HTRA3 31
  • 76 (a)
 OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
 Amphibia htra3 30
  • 73.04 (n)
Zebrafish
(Danio rerio)
 Actinopterygii LOC573378 30
  • 69.06 (n)
HTRA3 (1 of 3) 31
  • 66 (a)
 OneToMany
htra3a 31
  • 64 (a)
 OneToMany
HTRA3 (3 of 3) 31
  • 64 (a)
 OneToMany
Fruit Fly
(Drosophila melanogaster)
 Insecta CG8464 32
  • 44 (a)
HtrA2 31
  • 37 (a)
 OneToMany
Sea Squirt
(Ciona savignyi)
 Ascidiacea -- 31
  • 42 (a)
 OneToMany
Species where no ortholog for HTRA3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for HTRA3 Gene

ENSEMBL:
Gene Tree for HTRA3 (if available)
TreeFam:
Gene Tree for HTRA3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for HTRA3: view image

Paralogs for HTRA3 Gene

Paralogs for HTRA3 Gene

(3) SIMAP similar genes for HTRA3 Gene using alignment to 1 proteins:

  • HTRA3_HUMAN
genes like me logo Genes that share paralogs with HTRA3: view

Variants for HTRA3 Gene

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dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for HTRA3 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for HTRA3 Gene

Variant ID Type Subtype PubMed ID
dgv8956n54 CNV gain 21841781
dgv8957n54 CNV gain 21841781
esv1430054 CNV insertion 17803354
esv1923424 CNV deletion 18987734
esv2493897 CNV insertion 19546169
esv2727109 CNV deletion 23290073
esv29195 CNV loss 19812545
esv34196 OTHER inversion 12058347
esv3569280 CNV loss 25503493
esv3575676 CNV gain 25503493
esv3599544 CNV loss 21293372
nsv1002794 CNV gain 25217958
nsv1009011 CNV loss 25217958
nsv1073303 CNV deletion 25765185
nsv1125239 CNV insertion 24896259
nsv1132929 CNV insertion 24896259
nsv1136815 CNV deletion 24896259
nsv1142385 CNV tandem duplication 24896259
nsv1145025 CNV deletion 24896259
nsv4222 CNV insertion 18451855
nsv461216 CNV loss 19166990
nsv461218 CNV loss 19166990
nsv593626 CNV loss 21841781
nsv593627 CNV gain 21841781
nsv593628 CNV loss 21841781
nsv593634 CNV loss 21841781
nsv593635 CNV gain+loss 21841781
nsv593636 CNV loss 21841781
nsv829853 CNV gain+loss 17160897
nsv949784 CNV deletion 24416366

Variation tolerance for HTRA3 Gene

Residual Variation Intolerance Score: 34% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.85; 48.11% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for HTRA3 Gene

Human Gene Mutation Database (HGMD)
HTRA3
SNPedia medical, phenotypic, and genealogical associations of SNPs for
HTRA3

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for HTRA3 Gene

Disorders for HTRA3 Gene

Additional Disease Information for HTRA3

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform

No disorders were found for HTRA3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for HTRA3 Gene

Publications for HTRA3 Gene

  1. Identification and cloning of two isoforms of human high-temperature requirement factor A3 (HtrA3), characterization of its genomic structure and comparison of its tissue distribution with HtrA1 and HtrA2. (PMID: 12513693) Nie GY … Salamonsen LA (The Biochemical journal 2003) 2 3 4 23
  2. Expression of human HtrA1, HtrA2, HtrA3 and TGF-beta1 genes in primary endometrial cancer. (PMID: 19424634) Narkiewicz J … Lipinska B (Oncology reports 2009) 3 4 23
  3. Serine proteases HTRA1 and HTRA3 are down-regulated with increasing grades of human endometrial cancer. (PMID: 16650464) Bowden MA … Nie G (Gynecologic oncology 2006) 3 4 23
  4. Structural and Functional Analysis of Human HtrA3 Protease and Its Subdomains. (PMID: 26110759) Glaza P … Lipinska B (PloS one 2015) 3 4
  5. Application of the wheat-germ cell-free translation system to produce high temperature requirement A3 (HtrA3) proteases. (PMID: 22229724) Singh H … Nie G (BioTechniques 2012) 3 4

ExternalLinks

View latest publications for HTRA3 gene in Mastermind

Products for HTRA3 Gene

  • Addgene plasmids for HTRA3

Sources for HTRA3 Gene