This gene encodes the enzyme heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4. This enzyme generates 3-O-sulfated glucosaminyl residues in heparan sulfate. Cell surface heparan sulfate is used as a receptor by herpes simplex virus type 1 (HSV-1), and expression of this gene is thought to play a role in HSV-1 pathogenesis. [provided by RefSeq, Jul 2008] See more...

Aliases for HS3ST4 Gene

Aliases for HS3ST4 Gene

  • Heparan Sulfate-Glucosamine 3-Sulfotransferase 4 2 3 5
  • 3OST4 2 3 4
  • Heparan Sulfate D-Glucosaminyl 3-O-Sulfotransferase 4 3 4
  • Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 4 2 3
  • Heparan Sulfate Glucosamine 3-O-Sulfotransferase 4 3 4
  • Heparan Sulfate 3-O-Sulfotransferase 4 3 4
  • H3-OST-4 3 4
  • 3-OST-4 3 4
  • EC 2.8.2.23 4
  • EC 2.8.2 51
  • HS3ST4 5
  • 30ST4 3

External Ids for HS3ST4 Gene

Previous GeneCards Identifiers for HS3ST4 Gene

  • GC16P025131
  • GC16P025802
  • GC16P025631
  • GC16P025669
  • GC16P025610
  • GC16P023796
  • GC16P025692
  • GC16P026136
  • GC16P026165
  • GC16P026277

Summaries for HS3ST4 Gene

Entrez Gene Summary for HS3ST4 Gene

  • This gene encodes the enzyme heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4. This enzyme generates 3-O-sulfated glucosaminyl residues in heparan sulfate. Cell surface heparan sulfate is used as a receptor by herpes simplex virus type 1 (HSV-1), and expression of this gene is thought to play a role in HSV-1 pathogenesis. [provided by RefSeq, Jul 2008]

GeneCards Summary for HS3ST4 Gene

HS3ST4 (Heparan Sulfate-Glucosamine 3-Sulfotransferase 4) is a Protein Coding gene. Diseases associated with HS3ST4 include Spherocytosis, Type 3 and Apical Myocardial Infarction. Among its related pathways are Cytochrome P450 - arranged by substrate type and heparan sulfate biosynthesis. Gene Ontology (GO) annotations related to this gene include sulfotransferase activity and [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity. An important paralog of this gene is HS3ST2.

UniProtKB/Swiss-Prot Summary for HS3ST4 Gene

  • Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for HS3ST4 Gene

Genomics for HS3ST4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for HS3ST4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J025691 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE CraniofacialAtlas 600.7 +0.2 242 2 POLR2A EZH2 RAD21 SPI1 TAF1 CHD2 TARDBP HS3ST4 HSALNG0110368 HSALNG0110370
GH16J025729 Enhancer 0.5 FANTOM5 Ensembl 15.1 +37.4 37442 0.8 CREB1 HS3ST4 ZKSCAN2 HSALNG0110370
GH16J025712 Enhancer 0.2 FANTOM5 13.9 +20.8 20813 0.4 HS3ST4 ZKSCAN2 HSALNG0110370
GH16J025790 Enhancer 0.2 FANTOM5 11.6 +98.5 98539 0 piR-50444-147 HS3ST4 ENSG00000285882 ENSG00000260255
GH16J025762 Enhancer 0.2 FANTOM5 9.5 +71.1 71128 0.3 HS3ST4 RF00017-2026 ENSG00000285882
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around HS3ST4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for HS3ST4

Top Transcription factor binding sites by QIAGEN in the HS3ST4 gene promoter:
  • AhR
  • AREB6
  • C/EBPalpha
  • CHOP-10
  • FOXJ2
  • FOXJ2 (long isoform)
  • HOXA5
  • HSF2
  • Ik-1
  • p300

Genomic Locations for HS3ST4 Gene

Genomic Locations for HS3ST4 Gene
chr16:25,691,959-26,137,685
(GRCh38/hg38)
Size:
445,727 bases
Orientation:
Plus strand
chr16:25,703,347-26,149,009
(GRCh37/hg19)
Size:
445,663 bases
Orientation:
Plus strand

Genomic View for HS3ST4 Gene

Genes around HS3ST4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
HS3ST4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for HS3ST4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for HS3ST4 Gene

Proteins for HS3ST4 Gene

  • Protein details for HS3ST4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y661-HS3S4_HUMAN
    Recommended name:
    Heparan sulfate glucosamine 3-O-sulfotransferase 4
    Protein Accession:
    Q9Y661
    Secondary Accessions:
    • Q5QI42
    • Q8NDC2

    Protein attributes for HS3ST4 Gene

    Size:
    456 amino acids
    Molecular mass:
    49799 Da
    Quaternary structure:
    No Data Available

neXtProt entry for HS3ST4 Gene

Selected DME Specific Peptides for HS3ST4 Gene

Q9Y661:
  • KGGTRALLE
  • EPHFFDR
  • PRCLGKSKGR
  • EKTPSYFVT
  • FYFNKTKGF
  • KLIVVVRNPVTRAISDYTQTLSKKP
  • TKGFPCLKK

Post-translational modifications for HS3ST4 Gene

  • Glycosylation at Asn105, Asn178, Asn318, and Asn389

Other Protein References for HS3ST4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for HS3ST4 Gene

Gene Families for HS3ST4 Gene

Protein Domains for HS3ST4 Gene

Suggested Antigen Peptide Sequences for HS3ST4 Gene

GenScript: Design optimal peptide antigens:
  • Heparan sulfate 3-O-sulfotransferase 4 (A5H458_HUMAN)
  • Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4 (HS3S4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9Y661

UniProtKB/Swiss-Prot:

HS3S4_HUMAN :
  • Belongs to the sulfotransferase 1 family.
Family:
  • Belongs to the sulfotransferase 1 family.
genes like me logo Genes that share domains with HS3ST4: view

Function for HS3ST4 Gene

Molecular function for HS3ST4 Gene

UniProtKB/Swiss-Prot Function:
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=3'-phosphoadenylyl sulfate + alpha-D-glucosaminyl-[heparan sulfate](n) = 3-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+); Xref=Rhea:RHEA:15461, Rhea:RHEA-COMP:9830, Rhea:RHEA-COMP:9831, ChEBI:CHEBI:15378, ChEBI:CHEBI:58339, ChEBI:CHEBI:58343, ChEBI:CHEBI:58388, ChEBI:CHEBI:70975; EC=2.8.2.23;.
GENATLAS Biochemistry:
heparan sulfate D-glucosamine 3-O-sulfotransferase 4,putative regulator of heparan sulfate proteoglycan properties

Enzyme Numbers (IUBMB) for HS3ST4 Gene

Phenotypes From GWAS Catalog for HS3ST4 Gene

Gene Ontology (GO) - Molecular Function for HS3ST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008146 sulfotransferase activity IEA --
GO:0008467 [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity IBA,NAS 9988767
GO:0016740 transferase activity IEA --
genes like me logo Genes that share ontologies with HS3ST4: view
genes like me logo Genes that share phenotypes with HS3ST4: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for HS3ST4

No data available for Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for HS3ST4 Gene

Localization for HS3ST4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for HS3ST4 Gene

Golgi apparatus membrane. Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for HS3ST4 gene
Compartment Confidence
golgi apparatus 3
mitochondrion 2
plasma membrane 1
extracellular 1
cytoskeleton 1
nucleus 1
endoplasmic reticulum 1
cytosol 1

Gene Ontology (GO) - Cellular Components for HS3ST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0005794 Golgi apparatus IEA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with HS3ST4: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for HS3ST4 Gene

Pathways & Interactions for HS3ST4 Gene

genes like me logo Genes that share pathways with HS3ST4: view

Interacting Proteins for HS3ST4 Gene

Gene Ontology (GO) - Biological Process for HS3ST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0030201 heparan sulfate proteoglycan metabolic process NAS 9988767
genes like me logo Genes that share ontologies with HS3ST4: view

No data available for SIGNOR curated interactions for HS3ST4 Gene

Drugs & Compounds for HS3ST4 Gene

(2) Drugs for HS3ST4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
3'-phospho-5'-adenylyl sulfate Experimental Pharma 0
Adenosine 3',5'-diphosphate Experimental Pharma 0

(1) Additional Compounds for HS3ST4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Iduronic acid
  • Iduronate
  • L-Iduronate
  • L-Iduronic acid
  • Acid, iduronic
3402-98-0
genes like me logo Genes that share compounds with HS3ST4: view

Transcripts for HS3ST4 Gene

mRNA/cDNA for HS3ST4 Gene

1 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for HS3ST4

Alternative Splicing Database (ASD) splice patterns (SP) for HS3ST4 Gene

No ASD Table

Relevant External Links for HS3ST4 Gene

GeneLoc Exon Structure for
HS3ST4

Expression for HS3ST4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for HS3ST4 Gene

mRNA differential expression in normal tissues according to GTEx for HS3ST4 Gene

This gene is overexpressed in Brain - Cortex (x8.3), Brain - Frontal Cortex (BA9) (x6.1), Brain - Hippocampus (x5.8), Brain - Anterior cingulate cortex (BA24) (x5.7), and Brain - Hypothalamus (x4.1).

Protein differential expression in normal tissues from HIPED for HS3ST4 Gene

This gene is overexpressed in Liver, secretome (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for HS3ST4 Gene



Protein tissue co-expression partners for HS3ST4 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for HS3ST4

SOURCE GeneReport for Unigene cluster for HS3ST4 Gene:

Hs.655275

mRNA Expression by UniProt/SwissProt for HS3ST4 Gene:

Q9Y661-HS3S4_HUMAN
Tissue specificity: Brain-specific.

Evidence on tissue expression from TISSUES for HS3ST4 Gene

  • Nervous system(4.5)
genes like me logo Genes that share expression patterns with HS3ST4: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for HS3ST4 Gene

Orthologs for HS3ST4 Gene

This gene was present in the common ancestor of chordates.

Orthologs for HS3ST4 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia HS3ST4 30
  • 99.68 (n)
Cow
(Bos Taurus)
Mammalia HS3ST4 30
  • 94.74 (n)
Dog
(Canis familiaris)
Mammalia HS3ST4 30
  • 91.92 (n)
Rat
(Rattus norvegicus)
Mammalia LOC691700 30
  • 87.92 (n)
Mouse
(Mus musculus)
Mammalia Hs3st4 30 17 31
  • 86.59 (n)
OneToOne
Chicken
(Gallus gallus)
Aves HS3ST4 30 31
  • 82.52 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 75 (a)
ManyToMany
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia LOC100492416 30
  • 74.59 (n)
Zebrafish
(Danio rerio)
Actinopterygii hs3st4 30 31
  • 67.76 (n)
OneToOne
Species where no ortholog for HS3ST4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for HS3ST4 Gene

ENSEMBL:
Gene Tree for HS3ST4 (if available)
TreeFam:
Gene Tree for HS3ST4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for HS3ST4: view image

Paralogs for HS3ST4 Gene

(9) SIMAP similar genes for HS3ST4 Gene using alignment to 2 proteins:

  • HS3S4_HUMAN
  • A5H458_HUMAN
genes like me logo Genes that share paralogs with HS3ST4: view

Variants for HS3ST4 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for HS3ST4 Gene

SNP ID Clinical significance and condition Chr 16 pos Variation AA Info Type
rs2943336 - p.Gln136Arg

Additional dbSNP identifiers (rs#s) for HS3ST4 Gene

Structural Variations from Database of Genomic Variants (DGV) for HS3ST4 Gene

Variant ID Type Subtype PubMed ID
dgv164n21 CNV loss 19592680
dgv497e214 CNV loss 21293372
esv1614178 CNV deletion 17803354
esv2666037 CNV deletion 23128226
esv2714126 CNV deletion 23290073
esv2714127 CNV deletion 23290073
esv2714128 CNV deletion 23290073
esv2714130 CNV deletion 23290073
esv2714131 CNV deletion 23290073
esv2758639 CNV gain 17122850
esv2761904 CNV loss 21179565
esv3198511 CNV deletion 24192839
esv3306818 CNV mobile element insertion 20981092
esv3375442 CNV insertion 20981092
esv3389302 CNV insertion 20981092
esv3452152 CNV duplication 20981092
esv3582116 CNV loss 25503493
esv3582117 CNV loss 25503493
esv3582118 CNV loss 25503493
esv3638279 CNV loss 21293372
esv3638284 CNV gain 21293372
esv3638288 CNV gain 21293372
esv3638289 CNV gain 21293372
esv3638290 CNV gain 21293372
esv3638291 CNV gain 21293372
esv3638293 CNV loss 21293372
esv3638294 CNV loss 21293372
esv3892819 CNV gain 25118596
esv8601 CNV gain 19470904
esv989105 CNV deletion 20482838
nsv1064793 CNV gain 25217958
nsv1067150 CNV loss 25217958
nsv1123024 CNV deletion 24896259
nsv1136210 CNV deletion 24896259
nsv1138420 CNV deletion 24896259
nsv476470 CNV novel sequence insertion 20440878
nsv480644 CNV novel sequence insertion 20440878
nsv480725 CNV novel sequence insertion 20440878
nsv480788 CNV novel sequence insertion 20440878
nsv507811 OTHER sequence alteration 20534489
nsv519437 CNV loss 19592680
nsv521162 CNV gain 19592680
nsv9407 CNV loss 18304495
nsv978120 CNV duplication 23825009

Variation tolerance for HS3ST4 Gene

Residual Variation Intolerance Score: 61.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.06; 60.78% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for HS3ST4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
HS3ST4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HS3ST4 Gene

Disorders for HS3ST4 Gene

MalaCards: The human disease database

(3) MalaCards diseases for HS3ST4 Gene - From: DISEASES

Disorder Aliases PubMed IDs
spherocytosis, type 3
  • hs3
apical myocardial infarction
hereditary spherocytosis
  • spherocytosis, type 1
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for HS3ST4

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with HS3ST4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for HS3ST4 Gene

Publications for HS3ST4 Gene

  1. Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cdnas and identification of distinct genomic loci. (PMID: 9988767) Shworak NW … Rosenberg RD (The Journal of biological chemistry 1999) 2 3 4 23
  2. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41
  3. Genome-wide studies of verbal declarative memory in nondemented older people: the Cohorts for Heart and Aging Research in Genomic Epidemiology consortium. (PMID: 25648963) Debette S … Cohorts for Heart and Aging Research in Genomic Epidemiology Consortium (Biological psychiatry 2015) 3
  4. Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects. (PMID: 24324551) Deng X … REDSII Chagas Study Group from the NHLBI Retrovirus Epidemiology Donor Study-II Component International (PloS one 2013) 3
  5. Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. (PMID: 23251661) Comuzzie AG … Butte NF (PloS one 2012) 3

Products for HS3ST4 Gene

Sources for HS3ST4 Gene