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Aliases for GPR35 Gene

Aliases for GPR35 Gene

  • G Protein-Coupled Receptor 35 2 3 5
  • Kynurenic Acid Receptor 3 4
  • KYNA Receptor 3 4
  • G-Protein Coupled Receptor 35 3

External Ids for GPR35 Gene

Previous GeneCards Identifiers for GPR35 Gene

  • GC02M239906
  • GC02M240750
  • GC02P241588
  • GC02P241889
  • GC02P241286
  • GC02P241215
  • GC02P233327

Summaries for GPR35 Gene

GeneCards Summary for GPR35 Gene

GPR35 (G Protein-Coupled Receptor 35) is a Protein Coding gene. Diseases associated with GPR35 include Cholangitis, Primary Sclerosing and Cholecystolithiasis. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity. An important paralog of this gene is GPR55.

UniProtKB/Swiss-Prot for GPR35 Gene

  • Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.

Gene Wiki entry for GPR35 Gene

Additional gene information for GPR35 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for GPR35 Gene

Genomics for GPR35 Gene

GeneHancer (GH) Regulatory Elements for GPR35 Gene

Promoters and enhancers for GPR35 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH02J240620 Promoter/Enhancer 1.9 EPDnew FANTOM5 Ensembl ENCODE 672.4 +18.6 18585 7.2 PKNOX1 MLX ARID4B DMAP1 IRF4 ZNF48 ZNF121 ZNF143 ATF7 RUNX3 GPR35 DUSP28 CAPN10 LOC285191 PASK AQP12B AQP12A MTERF4 RNPEPL1 LOC100420500
GH02J240603 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE dbSUPER 656.3 -0.1 -73 3.1 PKNOX1 SMAD1 FOXA2 MLX ARID4B DMAP1 SLC30A9 ZNF143 DEK PAF1 GPR35 ANKMY1 ENSG00000223374 FARP2 CAPN10
GH02J240583 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 16.3 -19.7 -19683 4.5 HDGF PKNOX1 FOXA2 SMAD1 ARNT ARID4B SIN3A DMAP1 ZNF2 IRF4 CAPN10 CAPN10-DT PIR33196 GPR35
GH02J240596 Enhancer 0.8 ENCODE 16 -7.7 -7744 1.5 ELF3 SOX13 TFAP4 FOXA2 SAP130 MLX ARID4B THRB RBPJ RARA GPR35 CAPN10
GH02J240023 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 5 -580.3 -580347 2.7 HDGF ARID4B SIN3A DMAP1 ZBTB7B IRF4 POLR2B ZNF207 ZNF143 SP3 NDUFA10 GPR35 PIR31958 GC02M240027
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around GPR35 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the GPR35 gene promoter:
  • ATF
  • USF1
  • USF-1
  • Max
  • c-Myc
  • MIF-1
  • HOXA5
  • STAT5A
  • p300
  • ER-alpha

Genomic Locations for GPR35 Gene

Genomic Locations for GPR35 Gene
25,852 bases
Plus strand
25,852 bases
Plus strand

Genomic View for GPR35 Gene

Genes around GPR35 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
GPR35 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for GPR35 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for GPR35 Gene

Proteins for GPR35 Gene

  • Protein details for GPR35 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    G-protein coupled receptor 35
    Protein Accession:
    Secondary Accessions:
    • J3KR30
    • O43495
    • Q17R58
    • Q4VBN5
    • Q4ZFV2
    • Q6FHI8
    • Q86UH4

    Protein attributes for GPR35 Gene

    309 amino acids
    Molecular mass:
    34072 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for GPR35 Gene


neXtProt entry for GPR35 Gene

Post-translational modifications for GPR35 Gene

  • Glycosylation at Asn2
  • Modification sites at PhosphoSitePlus

Other Protein References for GPR35 Gene

No data available for DME Specific Peptides for GPR35 Gene

Domains & Families for GPR35 Gene

Gene Families for GPR35 Gene

Human Protein Atlas (HPA):
  • G-protein coupled receptors
  • Predicted membrane proteins
  • Transporters

Protein Domains for GPR35 Gene


Suggested Antigen Peptide Sequences for GPR35 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the G-protein coupled receptor 1 family.
  • Belongs to the G-protein coupled receptor 1 family.
genes like me logo Genes that share domains with GPR35: view

Function for GPR35 Gene

Molecular function for GPR35 Gene

UniProtKB/Swiss-Prot Function:
Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.
GENATLAS Biochemistry:
G protein coupled receptor 35,expressed in heart,spleen,liver,lung,ovary kidney

Phenotypes From GWAS Catalog for GPR35 Gene

Gene Ontology (GO) - Molecular Function for GPR35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004930 G-protein coupled receptor activity IDA,TAS 16754668
GO:0016494 C-X-C chemokine receptor activity IMP 25411203
genes like me logo Genes that share ontologies with GPR35: view
genes like me logo Genes that share phenotypes with GPR35: view

Human Phenotype Ontology for GPR35 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Model Products

Clone Products

  • Addgene plasmids for GPR35

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for GPR35 Gene

Localization for GPR35 Gene

Subcellular locations from UniProtKB/Swiss-Prot for GPR35 Gene

Cell membrane; Multi-pass membrane protein. Note=Internalized to the cytoplasm after exposure to kynurenic acid.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for GPR35 gene
Compartment Confidence
plasma membrane 5

Gene Ontology (GO) - Cellular Components for GPR35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane IDA,IEA 16754668
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with GPR35: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for GPR35 Gene

Pathways & Interactions for GPR35 Gene

genes like me logo Genes that share pathways with GPR35: view

Pathways by source for GPR35 Gene

1 KEGG pathway for GPR35 Gene

Gene Ontology (GO) - Biological Process for GPR35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007010 cytoskeleton organization IEA --
GO:0007165 signal transduction IEA --
GO:0007186 G-protein coupled receptor signaling pathway TAS,IDA 16754668
GO:0007204 positive regulation of cytosolic calcium ion concentration IMP 25411203
GO:0035025 positive regulation of Rho protein signal transduction IBA --
genes like me logo Genes that share ontologies with GPR35: view

No data available for SIGNOR curated interactions for GPR35 Gene

Drugs & Compounds for GPR35 Gene

(13) Drugs for GPR35 Gene - From: DrugBank, DGIdb, IUPHAR, and Tocris

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Bumetanide Approved Pharma Agonist, Inhibition, Inhibitor, blocker NKCC cotransporter inhibitor, Na+/2Cl-/K+ (NKCC) symporter inhibitor 19
Furosemide Approved, Vet_approved Pharma Agonist, Inhibition, Inhibitor, blocker GABAA receptors antagonist, Na+/2Cl-/K+ (NKCC) symporter inhibitor. Also antagonizes GABAA 209
Pyrantel Approved, Vet_approved Pharma Target 19
Kynurenic acid Investigational Pharma Agonist, Target 4
Zaprinast Pharma Agonist, Inhibition, Inhibitor phosphodiesterase inhibitor, GPR35 agonist 0

(5) Tocris Compounds for GPR35 Gene

Compound Action Cas Number
Pamoic acid disodium salt GPR35 agonist 6640-22-8
TC-G 1001 GPR35 agonist; more potent than zaprinast (Cat. No. 0947) 494191-73-0
TCS 3035 GPR35 agonist 871085-49-3
YE 120 GPR35 agonist 383124-82-1
Zaprinast GPR35 agonist 37762-06-4
genes like me logo Genes that share compounds with GPR35: view

Transcripts for GPR35 Gene

mRNA/cDNA for GPR35 Gene

Unigene Clusters for GPR35 Gene

G protein-coupled receptor 35:
Representative Sequences:

Clone Products

  • Addgene plasmids for GPR35

Alternative Splicing Database (ASD) splice patterns (SP) for GPR35 Gene

No ASD Table

Relevant External Links for GPR35 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for GPR35 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for GPR35 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for GPR35 Gene

This gene is overexpressed in Colon - Transverse (x8.6) and Small Intestine - Terminal Ileum (x6.4).

NURSA nuclear receptor signaling pathways regulating expression of GPR35 Gene:


SOURCE GeneReport for Unigene cluster for GPR35 Gene:


mRNA Expression by UniProt/SwissProt for GPR35 Gene:

Tissue specificity: Predominantly expressed in immune and gastrointestinal tissues.

Evidence on tissue expression from TISSUES for GPR35 Gene

  • Nervous system(4.3)
  • Intestine(3.8)

Phenotype-based relationships between genes and organs from Gene ORGANizer for GPR35 Gene

Germ Layers:
  • ectoderm
  • endoderm
  • mesoderm
  • digestive
  • integumentary
  • nervous
  • urinary
Head and neck:
  • eye
  • head
  • mouth
  • abdominal wall
  • biliary tract
  • gallbladder
  • kidney
  • liver
  • pancreas
  • ureter
  • urinary bladder
  • skin
genes like me logo Genes that share expression patterns with GPR35: view

No data available for Protein differential expression in normal tissues , Protein expression and Protein tissue co-expression partners for GPR35 Gene

Orthologs for GPR35 Gene

This gene was present in the common ancestor of chordates.

Orthologs for GPR35 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia GPR35 34 33
  • 97.05 (n)
(Canis familiaris)
Mammalia LOC100855802 33
  • 79.47 (n)
GPR35 34
  • 65 (a)
(Bos Taurus)
Mammalia GPR35 34 33
  • 78.88 (n)
(Mus musculus)
Mammalia Gpr35 16 34 33
  • 78.18 (n)
(Rattus norvegicus)
Mammalia Gpr35 33
  • 77.26 (n)
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 45 (a)
-- 34
  • 35 (a)
-- 34
  • 35 (a)
(Monodelphis domestica)
Mammalia GPR35 34
  • 38 (a)
(Gallus gallus)
Aves GPR35 33
  • 51.79 (n)
-- 34
  • 37 (a)
-- 34
  • 29 (a)
(Anolis carolinensis)
Reptilia -- 34
  • 36 (a)
-- 34
  • 30 (a)
(Danio rerio)
Actinopterygii GPR35 (1 of 3) 34
  • 28 (a)
GPR35 (2 of 3) 34
  • 27 (a)
GPR35 (3 of 3) 34
  • 26 (a)
Species where no ortholog for GPR35 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for GPR35 Gene

Gene Tree for GPR35 (if available)
Gene Tree for GPR35 (if available)
Evolutionary constrained regions (ECRs) for GPR35: view image

Paralogs for GPR35 Gene

Paralogs for GPR35 Gene

(9) SIMAP similar genes for GPR35 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with GPR35: view

Variants for GPR35 Gene

Sequence variations from dbSNP and Humsavar for GPR35 Gene

SNP ID Clin Chr 02 pos Variation AA Info Type
rs1000001290 -- 240,615,618(+) G/A genic_upstream_transcript_variant, intron_variant
rs1000238975 -- 240,628,882(+) G/A intron_variant
rs1000384083 -- 240,605,328(+) C/T upstream_transcript_variant
rs1000420757 -- 240,613,243(+) C/G genic_upstream_transcript_variant, intron_variant
rs1000585791 -- 240,616,340(+) G/A genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for GPR35 Gene

Variant ID Type Subtype PubMed ID
esv1170354 CNV insertion 17803354
esv1425277 CNV insertion 17803354
esv2721937 CNV deletion 23290073
esv2721938 CNV deletion 23290073
esv2721939 CNV deletion 23290073
esv2759119 CNV gain 17122850
esv29214 CNV loss 19812545
esv3389950 CNV duplication 20981092
esv3430269 CNV duplication 20981092
esv3594806 CNV loss 21293372
nsv1001262 CNV gain 25217958
nsv10227 CNV gain 18304495
nsv1130764 CNV deletion 24896259
nsv1136493 CNV deletion 24896259
nsv1142405 CNV deletion 24896259
nsv1147160 CNV deletion 26484159
nsv3240 CNV insertion 18451855
nsv460159 CNV loss 19166990
nsv470529 CNV loss 18288195
nsv584812 CNV gain 21841781
nsv584813 CNV gain 21841781
nsv584814 CNV gain 21841781
nsv584815 CNV loss 21841781
nsv820464 CNV duplication 20802225
nsv834586 CNV loss 17160897
nsv955292 CNV deletion 24416366

Variation tolerance for GPR35 Gene

Residual Variation Intolerance Score: 88% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.51; 64.57% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for GPR35 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for GPR35 Gene

Disorders for GPR35 Gene

MalaCards: The human disease database

(2) MalaCards diseases for GPR35 Gene - From: HGMD, Orphanet, and DISEASES

Disorder Aliases PubMed IDs
cholangitis, primary sclerosing
  • psc
- elite association - COSMIC cancer census association via MalaCards
Search GPR35 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for GPR35

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with GPR35: view

No data available for UniProtKB/Swiss-Prot and Genatlas for GPR35 Gene

Publications for GPR35 Gene

  1. Discovery of three novel G-protein-coupled receptor genes. (PMID: 9479505) O'Dowd BF … George SR (Genomics 1998) 2 3 4 58
  2. Meta-analysis identifies 29 additional ulcerative colitis risk loci, increasing the number of confirmed associations to 47. (PMID: 21297633) Anderson CA … Rioux JD (Nature genetics 2011) 3 44 58
  3. Application of machine learning algorithms to predict coronary artery calcification with a sibship-based design. (PMID: 18271057) Sun YV … Kardia SL (Genetic epidemiology 2008) 3 44 58
  4. Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35. (PMID: 16754668) Wang J … Ling L (The Journal of biological chemistry 2006) 3 4 58
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58

Products for GPR35 Gene

Sources for GPR35 Gene

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