Aliases for GPR18 Gene

Aliases for GPR18 Gene

  • G Protein-Coupled Receptor 18 2 3 5
  • N-Arachidonyl Glycine Receptor 3 4
  • NAGly Receptor 3 4
  • G-Protein Coupled Receptor 18 4
  • GPR18 5
  • GPCRW 4

External Ids for GPR18 Gene

Previous GeneCards Identifiers for GPR18 Gene

  • GC13M098266
  • GC13M094294
  • GC13M098743
  • GC13M097604
  • GC13M098704
  • GC13M099906
  • GC13M080501

Summaries for GPR18 Gene

GeneCards Summary for GPR18 Gene

GPR18 (G Protein-Coupled Receptor 18) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and GPCRs, Other. Gene Ontology (GO) annotations related to this gene include G protein-coupled receptor activity. An important paralog of this gene is GPR17.

UniProtKB/Swiss-Prot Summary for GPR18 Gene

  • Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (PubMed:16844083, PubMed:24762058, PubMed:27572937). However, conflicting results about the role of NAGly as an agonist are reported (PubMed:27018161). Can also be activated by plant-derived and synthetic cannabinoid agonists (PubMed:24762058). The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (PubMed:16844083). May contribute to regulation of the immune system. Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs)in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity). Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (PubMed:27572937).

Gene Wiki entry for GPR18 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for GPR18 Gene

Genomics for GPR18 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for GPR18 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH13J099255 Promoter/Enhancer 1.6 EPDnew Ensembl ENCODE dbSUPER 605.2 +4.6 4611 5.8 SP1 JUND RELA IKZF2 POLR2A RCOR1 TRIM22 PKNOX1 EED ARID3A GPR18 MIR623 UBAC2 lnc-TM9SF2-7 piR-55281-150
GH13J099261 Enhancer 0.7 ENCODE dbSUPER 600.7 +1.2 1236 0.2 RCOR2 SOX13 CTCF EBF1 SMAD4 HNF4A KDM1A NR2F6 SOX5 SMC3 GPR18 RF00017-1477 lnc-TM9SF2-7 piR-55281-150 UBAC2
GH13J099264 Promoter/Enhancer 0.5 Ensembl dbSUPER 600.7 -0.7 -703 0.4 GPR18 RF00017-1477 lnc-TM9SF2-7 lnc-TM9SF2-6 GPR183 UBAC2
GH13J099263 Enhancer 0.4 ENCODE dbSUPER 600.7 -1.8 -1840 1.1 SPI1 GPR18 RF00017-1477 MIR623 DOCK9 lnc-TM9SF2-6 GPR183 UBAC2
GH13J099431 Promoter/Enhancer 1.9 FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 16.2 -171.3 -171330 5 SP1 TEAD4 LEF1 POLR2A BACH1 JUND LARP7 STAT3 GABPA ZNF143 ENSG00000285448 LOC107984558 GPR18 LINC00449 MIR623 GPR183 HSALNG0098913 TM9SF2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around GPR18 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for GPR18

Top Transcription factor binding sites by QIAGEN in the GPR18 gene promoter:
  • AhR
  • AML1a
  • Arnt
  • C/EBPalpha
  • Elk-1
  • p53
  • PPAR-gamma1
  • PPAR-gamma2
  • RORalpha1
  • SEF-1 (1)

Genomic Locations for GPR18 Gene

Genomic Locations for GPR18 Gene
chr13:99,254,713-99,262,498
(GRCh38/hg38)
Size:
7,786 bases
Orientation:
Minus strand
chr13:99,906,967-99,913,998
(GRCh37/hg19)
Size:
7,032 bases
Orientation:
Minus strand

Genomic View for GPR18 Gene

Genes around GPR18 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
GPR18 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for GPR18 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for GPR18 Gene

Proteins for GPR18 Gene

  • Protein details for GPR18 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q14330-GPR18_HUMAN
    Recommended name:
    N-arachidonyl glycine receptor
    Protein Accession:
    Q14330
    Secondary Accessions:
    • Q6GTM3
    • Q96HI6
    • Q9H2L2

    Protein attributes for GPR18 Gene

    Size:
    331 amino acids
    Molecular mass:
    38134 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAG44671.1; Type=Frameshift; Evidence={ECO:0000305};

neXtProt entry for GPR18 Gene

Post-translational modifications for GPR18 Gene

  • Glycosylation at Asn14
  • Modification sites at PhosphoSitePlus

Other Protein References for GPR18 Gene

No data available for DME Specific Peptides for GPR18 Gene

Domains & Families for GPR18 Gene

Gene Families for GPR18 Gene

Protein Domains for GPR18 Gene

Suggested Antigen Peptide Sequences for GPR18 Gene

GenScript: Design optimal peptide antigens:
  • G-protein coupled receptor 18 (GPR18_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q14330

UniProtKB/Swiss-Prot:

GPR18_HUMAN :
  • Belongs to the G-protein coupled receptor 1 family.
Family:
  • Belongs to the G-protein coupled receptor 1 family.
genes like me logo Genes that share domains with GPR18: view

Function for GPR18 Gene

Molecular function for GPR18 Gene

UniProtKB/Swiss-Prot Function:
Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (PubMed:16844083, PubMed:24762058, PubMed:27572937). However, conflicting results about the role of NAGly as an agonist are reported (PubMed:27018161). Can also be activated by plant-derived and synthetic cannabinoid agonists (PubMed:24762058). The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (PubMed:16844083). May contribute to regulation of the immune system. Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs)in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity). Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (PubMed:27572937).
GENATLAS Biochemistry:
G protein coupled receptor 18,abundantly expressed in testis and spleen

Phenotypes From GWAS Catalog for GPR18 Gene

Gene Ontology (GO) - Molecular Function for GPR18 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004930 G protein-coupled receptor activity IBA,NAS 9205118
genes like me logo Genes that share ontologies with GPR18: view
genes like me logo Genes that share phenotypes with GPR18: view

Animal Models for GPR18 Gene

MGI Knock Outs for GPR18:

Animal Model Products

  • Taconic Biosciences Mouse Models for GPR18

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for GPR18

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for GPR18 Gene

Localization for GPR18 Gene

Subcellular locations from UniProtKB/Swiss-Prot for GPR18 Gene

Cell membrane. Multi-pass membrane protein. Cytoplasmic vesicle membrane.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for GPR18 gene
Compartment Confidence
plasma membrane 5
extracellular 2
peroxisome 2
nucleus 2
cytoskeleton 1
mitochondrion 1
endoplasmic reticulum 1
cytosol 1
lysosome 1

Gene Ontology (GO) - Cellular Components for GPR18 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005623 cell IEA --
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane IBA 21873635
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane NAS 9205118
genes like me logo Genes that share ontologies with GPR18: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for GPR18 Gene

Pathways & Interactions for GPR18 Gene

genes like me logo Genes that share pathways with GPR18: view

Pathways by source for GPR18 Gene

Gene Ontology (GO) - Biological Process for GPR18 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002300 CD8-positive, alpha-beta intraepithelial T cell differentiation IEA --
GO:0002305 CD8-positive, gamma-delta intraepithelial T cell differentiation IEA --
GO:0002689 negative regulation of leukocyte chemotaxis IEA --
GO:0007165 signal transduction IEA --
GO:0007186 G protein-coupled receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with GPR18: view

No data available for SIGNOR curated interactions for GPR18 Gene

Drugs & Compounds for GPR18 Gene

(11) Drugs for GPR18 Gene - From: DrugBank, DGIdb, IUPHAR, and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
cannabidiol Approved, Investigational Pharma Agonist, Partial agonist, Antagonist, Channel blocker, Activator, Target 0
Glycine Approved, Vet_approved Nutra Agonist, Full agonist, Target, substrate 196
Medical Cannabis Experimental, Investigational Pharma Target 0
Nabiximols Investigational Pharma Target 59
&Delta;<sup>9</sup>-tetrahydrocannabinol Pharma Agonist, Partial agonist, Allosteric regulator, Potentiation, Full agonist, Activator, Channel blocker 0

(1) Additional Compounds for GPR18 Gene - From: IUPHAR

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
arachidonyl cyclopropylamide
Agonist
genes like me logo Genes that share compounds with GPR18: view

Transcripts for GPR18 Gene

mRNA/cDNA for GPR18 Gene

2 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for GPR18

Alternative Splicing Database (ASD) splice patterns (SP) for GPR18 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b
SP1:
SP2: -

Relevant External Links for GPR18 Gene

GeneLoc Exon Structure for
GPR18

Expression for GPR18 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for GPR18 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for GPR18 Gene

This gene is overexpressed in Testis (x13.1), Whole Blood (x11.2), and Spleen (x9.5).

Protein differential expression in normal tissues from HIPED for GPR18 Gene

This gene is overexpressed in Blymphocyte (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for GPR18 Gene



Protein tissue co-expression partners for GPR18 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for GPR18

SOURCE GeneReport for Unigene cluster for GPR18 Gene:

Hs.741589

mRNA Expression by UniProt/SwissProt for GPR18 Gene:

Q14330-GPR18_HUMAN
Tissue specificity: Expressed in midpiece of spermatozoon (at protein level) (PubMed:27572937). Most abundant in testis and spleen (PubMed:16844083). Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells (PubMed:16844083).

Evidence on tissue expression from TISSUES for GPR18 Gene

  • Spleen(4.5)
  • Blood(4.5)
  • Lymph node(4.5)
  • Intestine(2.3)
genes like me logo Genes that share expression patterns with GPR18: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for GPR18 Gene

Orthologs for GPR18 Gene

This gene was present in the common ancestor of chordates.

Orthologs for GPR18 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia GPR18 30 31
  • 99.9 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia GPR18 30 31
  • 89.73 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia GPR18 30 31
  • 87.71 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia GPR18 31
  • 85 (a)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Gpr18 30
  • 83.69 (n)
Mouse
(Mus musculus)
Mammalia Gpr18 30 17 31
  • 82.68 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 77 (a)
OneToMany
-- 31
  • 66 (a)
OneToMany
Chicken
(Gallus gallus)
Aves GPR18 30 31
  • 73.29 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia GPR18 31
  • 74 (a)
OneToOne
Zebrafish
(Danio rerio)
Actinopterygii gpr18 30 31
  • 65.84 (n)
OneToOne
Species where no ortholog for GPR18 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for GPR18 Gene

ENSEMBL:
Gene Tree for GPR18 (if available)
TreeFam:
Gene Tree for GPR18 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for GPR18: view image

Paralogs for GPR18 Gene

(35) SIMAP similar genes for GPR18 Gene using alignment to 2 proteins:

  • GPR18_HUMAN
  • Q5JUH9_HUMAN
genes like me logo Genes that share paralogs with GPR18: view

Variants for GPR18 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for GPR18 Gene

SNP ID Clinical significance and condition Chr 13 pos Variation AA Info Type
770422 Likely Benign: not provided 99,255,314(-) G/T MISSENSE_VARIANT,INTRON_VARIANT

Additional dbSNP identifiers (rs#s) for GPR18 Gene

Structural Variations from Database of Genomic Variants (DGV) for GPR18 Gene

Variant ID Type Subtype PubMed ID
nsv1050094 CNV gain 25217958
nsv523519 CNV loss 19592680

Variation tolerance for GPR18 Gene

Residual Variation Intolerance Score: 28.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.92; 19.02% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for GPR18 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
GPR18

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for GPR18 Gene

Disorders for GPR18 Gene

No disorders were found for GPR18 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for GPR18 Gene

Publications for GPR18 Gene

  1. Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis. (PMID: 9205118) Gantz I … Yamada T (Genomics 1997) 2 3 4
  2. Characterization of non-olfactory GPCRs in human sperm with a focus on GPR18. (PMID: 27572937) Flegel C … Gisselmann G (Scientific reports 2016) 3 4
  3. Activation of GPR18 by cannabinoid compounds: a tale of biased agonism. (PMID: 24762058) Console-Bram L … Abood ME (British journal of pharmacology 2014) 3 4
  4. Identification of N-arachidonylglycine as the endogenous ligand for orphan G-protein-coupled receptor GPR18. (PMID: 16844083) Kohno M … Yasukawa M (Biochemical and biophysical research communications 2006) 3 4
  5. The DNA sequence and analysis of human chromosome 13. (PMID: 15057823) Dunham A … Ross MT (Nature 2004) 3 4

Products for GPR18 Gene

Sources for GPR18 Gene