The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced t... See more...

Aliases for GNLY Gene

Aliases for GNLY Gene

  • Granulysin 2 3 4 5
  • TLA519 2 3 4
  • NKG5 2 3 4
  • T-Lymphocyte Activation Gene 519 2 3
  • T-Cell Activation Protein 519 3 4
  • Lymphokine LAG-2 3 4
  • D2S69E 2 3
  • LAG-2 2 3
  • LAG2 3 4
  • Lymphocyte-Activation Gene 2 3
  • Protein NKG5 4
  • GNLY 5

External Ids for GNLY Gene

Previous HGNC Symbols for GNLY Gene

  • LAG2

Previous GeneCards Identifiers for GNLY Gene

  • GC02P085816
  • GC02P086215
  • GC02P085895
  • GC02P085834
  • GC02P085912

Summaries for GNLY Gene

Entrez Gene Summary for GNLY Gene

  • The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

GeneCards Summary for GNLY Gene

GNLY (Granulysin) is a Protein Coding gene. Diseases associated with GNLY include Erythema Multiforme and Kyphoscoliotic Heart Disease. Among its related pathways are Allograft rejection and Defensins.

UniProtKB/Swiss-Prot Summary for GNLY Gene

  • Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Gene Wiki entry for GNLY Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for GNLY Gene

Genomics for GNLY Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for GNLY Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH02J085683 Enhancer 0.8 dbSUPER 262.6 -0.2 -196 2.6 PLRG1 FOXA1 MCM3 NONO HES1 NBN EBF1 ESRRA E4F1 YBX1 GNLY SNORD94 USP39 ENSG00000228363 KDM3A TCF7L1 C2orf68 RNU1-38P ENSG00000229498 ATOH8
GH02J085693 Promoter/Enhancer 0.7 EPDnew ENCODE dbSUPER 267.2 +9.0 9004 1 GNLY SFTPB RNU1-38P ATOH8 ENSG00000229498 GPR160P1 lnc-C2orf68-2 NONHSAG028398.2
GH02J085709 Promoter/Enhancer 1.5 FANTOM5 Ensembl ENCODE dbSUPER 33.6 +26.6 26565 4.4 ATF3 YY1 GABPA FOXA2 RXRA RARA ZBTB33 JUND SPI1 PKNOX1 lnc-GNLY-1 GNLY ATOH8 GPR160P1 USP39 SFTPB ENSG00000228363 ENSG00000229498 KDM3A HSALNG0016527
GH02J085701 Enhancer 1.4 FANTOM5 ENCODE dbSUPER 23.5 +19.1 19128 6.4 BCLAF1 ZNF207 MYC ZNF654 IKZF1 SSRP1 JUND ZNF580 ATF2 CEBPA ATOH8 GNLY GPR160P1 SFTPB C2orf68 USP39 RNU1-38P ENSG00000229498 VAMP8 SH2D6
GH02J085680 Promoter/Enhancer 1.1 Ensembl ENCODE dbSUPER 15.2 -3.7 -3658 2.4 CEBPB RXRA ZNF316 USF2 HNF4A POLR2A SP1 TRIM24 TRIM28 NR2F2 LOC105374842 NONHSAG028398.2 GNLY RNU1-38P ATOH8 ENSG00000229498 SFTPB lnc-C2orf68-2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around GNLY on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for GNLY

Top Transcription factor binding sites by QIAGEN in the GNLY gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • GR
  • GR-alpha

Genomic Locations for GNLY Gene

Latest Assembly
chr2:85,685,175-85,698,854
(GRCh38/hg38)
Size:
13,680 bases
Orientation:
Plus strand

Previous Assembly
chr2:85,921,481-85,925,975
(GRCh37/hg19 by Entrez Gene)
Size:
4,495 bases
Orientation:
Plus strand

chr2:85,912,298-85,925,977
(GRCh37/hg19 by Ensembl)
Size:
13,680 bases
Orientation:
Plus strand

Genomic View for GNLY Gene

Genes around GNLY on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
GNLY Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for GNLY Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for GNLY Gene

Proteins for GNLY Gene

  • Protein details for GNLY Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P22749-GNLY_HUMAN
    Recommended name:
    Granulysin
    Protein Accession:
    P22749
    Secondary Accessions:
    • P09325
    • Q6GU08

    Protein attributes for GNLY Gene

    Size:
    145 amino acids
    Molecular mass:
    16374 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for GNLY Gene

    Alternative splice isoforms for GNLY Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for GNLY Gene

Post-translational modifications for GNLY Gene

  • A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for GNLY Gene

Domains & Families for GNLY Gene

Gene Families for GNLY Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Predicted secreted proteins
  • Transporters

Protein Domains for GNLY Gene

InterPro:
Blocks:
  • Saposin B

Suggested Antigen Peptide Sequences for GNLY Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ50974, moderately similar to Granulysin (B4DJJ4_HUMAN)
  • cDNA FLJ51776, highly similar to Granulysin (B4E3H9_HUMAN)
  • T-cell activation protein 519 (GNLY_HUMAN)
genes like me logo Genes that share domains with GNLY: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for GNLY Gene

Function for GNLY Gene

Molecular function for GNLY Gene

UniProtKB/Swiss-Prot Function:
Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
UniProtKB/Swiss-Prot Induction:
By T-cell growth factor and IL2/interleukin-2.
GENATLAS Biochemistry:
Granulysin,expressed by cytolytic T-lymphocytes and NK cells,located in cytotoxic granules,involved in immunity against intracellular infection against M tuberculosis and other pathogens

Phenotypes From GWAS Catalog for GNLY Gene

Gene Ontology (GO) - Molecular Function for GNLY Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 32296183
genes like me logo Genes that share ontologies with GNLY: view
genes like me logo Genes that share phenotypes with GNLY: view

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for GNLY

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for GNLY Gene

Localization for GNLY Gene

Subcellular locations from UniProtKB/Swiss-Prot for GNLY Gene

Secreted. Note=Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for GNLY gene
Compartment Confidence
extracellular 5
plasma membrane 2
cytoskeleton 2
mitochondrion 2
nucleus 2
endoplasmic reticulum 2
endosome 2
cytosol 2
lysosome 2
golgi apparatus 2

Gene Ontology (GO) - Cellular Components for GNLY Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005615 extracellular space TAS 2212946
GO:0044194 colocalizes_with cytolytic granule IBA,IDA 9756476
GO:0097013 phagocytic vesicle lumen TAS --
genes like me logo Genes that share ontologies with GNLY: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for GNLY Gene

Pathways & Interactions for GNLY Gene

PathCards logo

SuperPathways for GNLY Gene

genes like me logo Genes that share pathways with GNLY: view

Pathways by source for GNLY Gene

1 BioSystems pathway for GNLY Gene
3 Reactome pathways for GNLY Gene

Interacting Proteins for GNLY Gene

Gene Ontology (GO) - Biological Process for GNLY Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006968 cellular defense response IDA 9756476
GO:0019730 antimicrobial humoral response TAS --
GO:0031640 killing of cells of other organism IBA,IDA 9756476
GO:0042742 defense response to bacterium IEA,IDA 9756476
GO:0050832 defense response to fungus IEA --
genes like me logo Genes that share ontologies with GNLY: view

No data available for SIGNOR curated interactions for GNLY Gene

Drugs & Compounds for GNLY Gene

(11) Drugs for GNLY Gene - From: DrugBank and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
3-(N-morpholino)propanesulfonic acid Experimental Pharma Target 0

(6) Additional Compounds for GNLY Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with GNLY: view

Transcripts for GNLY Gene

mRNA/cDNA for GNLY Gene

3 REFSEQ mRNAs :
16 NCBI additional mRNA sequence :
14 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for GNLY

Alternative Splicing Database (ASD) splice patterns (SP) for GNLY Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b · 6c · 6d · 6e · 6f ^ 7 ^ 8 ^ 9 ^ 10a · 10b
SP1: - - - - - - - - - - - - -
SP2: - - - - - - -
SP3: - - - - - - - - -
SP4: - - -
SP5: - - - - - -
SP6: - -
SP7: - - - - - - - - -
SP8: - - - - - -
SP9: - - - -
SP10:
SP11:
SP12: - -
SP13:

Relevant External Links for GNLY Gene

GeneLoc Exon Structure for
GNLY

Expression for GNLY Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for GNLY Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for GNLY Gene

This gene is overexpressed in Whole Blood (x41.8).

Protein differential expression in normal tissues from HIPED for GNLY Gene

This gene is overexpressed in NK cells (38.9) and Peripheral blood mononuclear cells (22.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for GNLY Gene



Protein tissue co-expression partners for GNLY Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for GNLY

SOURCE GeneReport for Unigene cluster for GNLY Gene:

Hs.105806

mRNA Expression by UniProt/SwissProt for GNLY Gene:

P22749-GNLY_HUMAN
Tissue specificity: Expressed in natural killer and T-cells.

Evidence on tissue expression from TISSUES for GNLY Gene

  • Blood(3.4)
  • Spleen(3)
  • Bone marrow(2.9)
  • Lymph node(2.7)
  • Lung(2.5)
  • Intestine(2.2)
  • Stomach(2)
genes like me logo Genes that share expression patterns with GNLY: view

Primer products for research

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for GNLY Gene

Orthologs for GNLY Gene

This gene was present in the common ancestor of mammals.

Orthologs for GNLY Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia GNLY 29 30
  • 97.47 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia -- 30
  • 38 (a)
OneToMany
-- 30
  • 38 (a)
OneToMany
-- 30
  • 30 (a)
OneToMany
Dog
(Canis familiaris)
Mammalia -- 30
  • 30 (a)
OneToMany
-- 30
  • 30 (a)
OneToMany
Species where no ortholog for GNLY was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Mouse (Mus musculus)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rat (Rattus norvegicus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)
  • Zebrafish (Danio rerio)

Evolution for GNLY Gene

ENSEMBL:
Gene Tree for GNLY (if available)
TreeFam:
Gene Tree for GNLY (if available)
Aminode:
Evolutionary constrained regions (ECRs) for GNLY: view image
Alliance of Genome Resources:
Additional Orthologs for GNLY

Paralogs for GNLY Gene

No data available for Paralogs for GNLY Gene

Variants for GNLY Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for GNLY Gene

SNP ID Clinical significance and condition Chr 02 pos Variation AA Info Type
rs10181739 Benign: not provided 85,694,429(+) G/A
NM_006433.5(GNLY):c.11G>A (p.Trp4Ter)
NONSENSE,FIVE_PRIME_UTR
rs151117270 Benign: not provided 85,694,426(+) C/T
NM_006433.5(GNLY):c.8C>T (p.Thr3Ile)
MISSENSE_VARIANT,FIVE_PRIME_UTR
rs6732835 Benign: not provided 85,695,345(+) T/C
NM_006433.5(GNLY):c.78T>C (p.Pro26=)
SYNONYMOUS
rs11127 -- p.Thr119Ile

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for GNLY Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for GNLY Gene

Variant ID Type Subtype PubMed ID
esv23053 CNV gain 19812545
nsv2812 CNV deletion 18451855
nsv582369 CNV loss 21841781

Variation tolerance for GNLY Gene

Residual Variation Intolerance Score: 93.3% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for GNLY Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
GNLY
Leiden Open Variation Database (LOVD)
GNLY

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for GNLY Gene

Disorders for GNLY Gene

MalaCards: The human disease database

(18) MalaCards diseases for GNLY Gene - From: COP and GCD

Disorder Aliases PubMed IDs
erythema multiforme
  • dermatostomatitis, erythema multiforme type
kyphoscoliotic heart disease
erythematosquamous dermatosis
  • other erythematosquamous dermatosis
folliculitis
mycobacterium tuberculosis 1
  • mycobacterium tuberculosis, susceptibility to, 1
- elite association - COSMIC cancer census association via MalaCards
Search GNLY in MalaCards View complete list of genes associated with diseases

Additional Disease Information for GNLY

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with GNLY: view

No data available for UniProtKB/Swiss-Prot and Genatlas for GNLY Gene

Publications for GNLY Gene

  1. Granulysin crystal structure and a structure-derived lytic mechanism. (PMID: 12488100) Anderson DH … Eisenberg D (Journal of molecular biology 2003) 3 4 22
  2. Granulysin: a novel antimicrobial peptide of cytolytic T lymphocytes and natural killer cells. (PMID: 10644038) Krensky AM (Biochemical pharmacology 2000) 3 4 22
  3. Biosynthesis of granulysin, a novel cytolytic molecule. (PMID: 10449094) Hanson DA … Krensky AM (Molecular immunology 1999) 3 4 22
  4. An antimicrobial activity of cytolytic T cells mediated by granulysin. (PMID: 9756476) Stenger S … Modlin RL (Science (New York, N.Y.) 1998) 3 4 22
  5. A cDNA clone expressed in natural killer and T cells that likely encodes a secreted protein. (PMID: 2212946) Yabe T … Houchins JP (The Journal of experimental medicine 1990) 2 3 4

Products for GNLY Gene

Sources for GNLY Gene