Aliases for FBXW7 Gene
Aliases for FBXW7 Gene
- F-Box And WD Repeat Domain Containing 7 2 3 5
- F-Box And WD Repeat Domain Containing 7, E3 Ubiquitin Protein Ligase 2 3
- F-Box And WD-40 Domain Protein 7 (Archipelago Homolog, Drosophila) 2 3
- F-Box Protein FBX30 3 4
- SEL-10 3 4
- FBX30 3 4
- SEL10 3 4
- HCdc4 3 4
- FBW7 3 4
- HAgo 3 4
- F-Box And WD-40 Domain-Containing Protein 7 4
- F-Box/WD Repeat-Containing Protein 7 3
External Ids for FBXW7 Gene
- HGNC: 16712
- Entrez Gene: 55294
- Ensembl: ENSG00000109670
- OMIM: 606278
- UniProtKB: Q969H0
Previous GeneCards Identifiers for FBXW7 Gene
- GC04M154093
- GC04M153631
- GC04M153708
- GC04M153820
- GC04M153821
- GC04M153600
- GC04M153461
- GC04M153242
- GC04M148968
Summaries for FBXW7 Gene
-
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
GeneCards Summary for FBXW7 Gene
FBXW7 (F-Box And WD Repeat Domain Containing 7) is a Protein Coding gene. Diseases associated with FBXW7 include Hyperlucent Lung and Lung Adenoid Cystic Carcinoma. Among its related pathways are Notch signaling pathway (KEGG) and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and ubiquitin protein ligase binding. An important paralog of this gene is ZNF106.
UniProtKB/Swiss-Prot for FBXW7 Gene
-
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923, PubMed:29149593, PubMed:25775507). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593).
Additional gene information for FBXW7 Gene
- Monarch Initiative
- Search for FBXW7 at DataMed
- Search for FBXW7 at HumanCyc
No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for FBXW7 Gene
Genomics for FBXW7 Gene
GeneHancer (GH) Regulatory Elements for FBXW7 Gene
Promoters and enhancers for FBXW7 Gene
| GeneHancer (GH) Identifier | GH Type | GH Score |
GH Sources | Gene Association Score | Total Score | TSS distance (kb) | Number of Genes Away | Size (kb) | Transcription Factor Binding Sites |
Gene Targets |
|---|---|---|---|---|---|---|---|---|---|---|
| GH04J152533 | Promoter/Enhancer | 2.3 | EPDnew Ensembl ENCODE dbSUPER | 650.7 | -0.1 | -144 | 4.7 | HDGF PKNOX1 CLOCK MLX ARNT ARID4B SIN3A FEZF1 DMAP1 ZNF2 | MIR4453HG MIR4453 FBXW7 | |
| GH04J152348 | Promoter/Enhancer | 1.9 | EPDnew Ensembl ENCODE | 650.1 | +185.0 | 184989 | 5.4 | PKNOX1 SIN3A FEZF1 ZNF2 GLIS2 ZNF143 ATF7 RUNX3 REST TSHZ1 | FBXW7 FBXW7-AS1 GC04P152362 | |
| GH04J152382 | Promoter/Enhancer | 1.4 | EPDnew Ensembl ENCODE | 650.1 | +153.4 | 153431 | 1 | PKNOX1 TAF1 | FBXW7 GC04M152372 GC04P152461 | |
| GH04J152679 | Promoter/Enhancer | 2.1 | EPDnew Ensembl ENCODE dbSUPER | 10.4 | -144.6 | -144621 | 4.2 | HDGF ATF1 PKNOX1 SIN3A IRF4 ETS1 ZNF143 ATF7 RUNX3 NBN | TMEM154 LOC105377495 FBXW7 GC04P152678 GC04M152687 | |
| GH04J152678 | Enhancer | 0.6 | FANTOM5 dbSUPER | 28.9 | -142.3 | -142322 | 0.4 | ZNF148 CEBPG | TMEM154 LOC105377495 GC04P152678 FBXW7 ARFIP1 |
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data dump
GeneHancers around FBXW7 on UCSC Golden Path with GeneCards custom track
- Top Transcription factor binding sites by QIAGEN in the FBXW7 gene promoter:
-
- c-Jun
- ATF-2
- AP-1
Regulatory Element Products
Genomic Locations for FBXW7 Gene
Genomic Locations for FBXW7 Gene
- chr4:152,320,544-152,536,095
- (GRCh38/hg38)
- Size:
- 215,552 bases
- Orientation:
- Minus strand
- chr4:153,242,410-153,457,253
- (GRCh37/hg19)
- Size:
- 214,844 bases
- Orientation:
- Minus strand
Genomic View for FBXW7 Gene
Genes around FBXW7 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 4q31.3 by Entrez Gene
- 4q31.3 by HGNC
- 4q31.3 by Ensembl
FBXW7 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for FBXW7 Gene
Proteins for FBXW7 Gene
-
Protein details for FBXW7 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- Q969H0-FBXW7_HUMAN
- Recommended name:
- F-box/WD repeat-containing protein 7
- Protein Accession:
- Q969H0
- B7ZLP9
- Q68DR0
- Q96A16
- Q96LE0
- Q96RI2
- Q9NUX6
Protein attributes for FBXW7 Gene
- Size:
- 707 amino acids
- Molecular mass:
- 79663 Da
- Quaternary structure:
-
- Homodimer; homodimerization plays a role in substrate binding and/or ubiquitination and degradation (PubMed:22608923, PubMed:17434132). Component of the SCF(FBXW7) complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts with PSEN1, cyclin-E (CCNE1 or CCNE2), NOTCH1 intracellular domain/NICD, NOTCH4 intracellular domain/NICD and SKP1. Interacts with MYC (when phosphorylated) (PubMed:17873522, PubMed:25775507). Isoform 1 interacts with USP28, leading to counteract ubiquitination of MYC (PubMed:17873522). Isoform 3 interacts (via WD repeats) with SV40 large T antigen (via CPD region). Forms a trimeric complex with NOTCH1 and SGK1. Interacts with JUN (PubMed:14739463, PubMed:22608923). Found in a complex with JUN and PRR7 (PubMed:27458189). Interacts with JUN and PRR7; the interaction inhibits ubiquitination-mediated JUN degradation promoting its phosphorylation and transcriptional activity (PubMed:27458189). Interacts (when phosphorylated at Thr-205) with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923). Interacts with UBE2QL1 (PubMed:24000165). Interacts with FAM83D; promotes FBXW7 degradation (PubMed:24344117). Interacts with MYCN; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN (PubMed:27837025). Isoform 3 interacts with STOML1 (PubMed:23082202). Interacts with NFE2L1 (By similarity). Interacts with NOTCH2 intracellular domain (N2ICD) (PubMed:29149593). Isoform 3 interacts with USP36, leading to counteract ubiquitination of MYC (PubMed:25775507).
- SequenceCaution:
-
- Sequence=BAA91986.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Three dimensional structures from OCA and Proteopedia for FBXW7 Gene
Protein Expression for FBXW7 Gene
Post-translational modifications for FBXW7 Gene
- Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).
- Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its degradation (PubMed:22608923).
- Modification sites at PhosphoSitePlus
- Modification sites at neXtProt
Other Protein References for FBXW7 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- R&D Systems Antibodies for FBXW7 (FBXW7/Cdc4)
- Novus Biologicals Antibodies for FBXW7
- Invitrogen Antibodies for FBXW7
- antibodies-online: Search results for 138 available FBXW7 Antibodies ranked by validation data
- Compare Top FBXW7 Antibodies
-
Recommended
- 12 IF (p) Antibodies (anti-rat)
- 11 WB Antibodies (anti-rat)
- 1 IP Antibody (anti-rat)
- 100 WB Antibodies (anti-human)
- 12 IF (p) Antibodies (anti-human)
- 6 IHC (p) Antibodies (anti-human)
- 5 IP Antibodies (anti-human)
- 2 FACS Antibodies (anti-human)
- 1 IHC Antibody (anti-human)
- 30 WB Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 2 FACS Antibodies (anti-mouse)
- 1 IHC Antibody (anti-mouse)
- 1 IP Antibody (anti-mouse)
- GeneTex FBXW7 Antibody for FBXW7
-
Custom Antibody ServicesProSci Antibodies for FBXW7
-
Santa Cruz Biotechnology (SCBT) Antibodies for FBXW7
Protein Products
-
OriGene Purified Proteins for FBXW7
- Search Origene for MassSpec and Protein Over-expression Lysates for FBXW7
- Origene Custom Protein Services for FBXW7
- Search GeneTex for Proteins for FBXW7
Assay Products
- R&D Systems Proteome Profiler Antibody Arrays and other biochemical assays for FBXW7 (FBXW7/Cdc4)
No data available for DME Specific Peptides for FBXW7 Gene
Domains & Families for FBXW7 Gene
Gene Families for FBXW7 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Cancer-related genes
- Predicted intracellular proteins
- Predicted secreted proteins
Protein Domains for FBXW7 Gene
Suggested Antigen Peptide Sequences for FBXW7 Gene
- GenScript: Design optimal peptide antigens:
Graphical View of Domain Structure for InterPro Entry
Q969H0UniProtKB/Swiss-Prot:
FBXW7_HUMAN :- The WD repeats mediate interaction with substrates of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
- Domain:
-
- The WD repeats mediate interaction with substrates of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
- The F-box domain mediates interaction with SKP1.
Function for FBXW7 Gene
Molecular function for FBXW7 Gene
- UniProtKB/Swiss-Prot Function:
- Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923, PubMed:29149593, PubMed:25775507). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593).
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0004842 | ubiquitin-protein transferase activity | TAS | -- |
| GO:0005515 | protein binding | IPI | 11533444 |
| GO:0030332 | cyclin binding | IPI | 17434132 |
| GO:0030674 | protein binding, bridging | IDA | 12628165 |
| GO:0031625 | ubiquitin protein ligase binding | IPI | 12628165 |
Phenotypes for FBXW7 Gene
- MGI mutant phenotypes for FBXW7:
-
inferred from 11 alleles
- cardiovascular system phenotype
- respiratory system phenotype
- vision/eye phenotype
- digestive/alimentary phenotype
- homeostasis/metabolism phenotype
- nervous system phenotype
- neoplasm
- integument phenotype
- endocrine/exocrine gland phenotype
- mortality/aging
- cellular phenotype
- craniofacial phenotype
- liver/biliary system phenotype
- embryo phenotype
- normal phenotype
- hematopoietic system phenotype
- immune system phenotype
- growth/size/body region phenotype
- GenomeRNAi human phenotypes for FBXW7:
-
- Decreased HPV16-GFP infection
- Increased shRNA abundance (Z-score > 2)
- Decreased CDKN1A mRNA expression
- Increased cell death HMECs cells
- Negative genetic interaction between PTTG1-/- and PTTG1+/+
- Synthetic lethal with c-Myc after tamoxifen stimulation
- Decreased viability
- no effect
- Increased Cyt C expression
- Increased vaccinia virus (VACV) infection
- Synthetic lethal with Ras
- shRNA abundance <= 50%
- Increased transferrin (TF) endocytosis
Animal Models for FBXW7 Gene
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
- Cyagen custom Knockout/knockin (KOKI) mouse models for FBXW7
-
CRISPR Products
-
OriGene CRISPR knockouts for FBXW7
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for FBXW7
- genomics-online: gRNA clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- Applied Biological Materials CRISPR for FBXW7
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for FBXW7
- GenScript: Design CRISPR guide RNA sequences for FBXW7
miRNA for FBXW7 Gene
- miRTarBase miRNAs that target FBXW7
-
- hsa-mir-197-3p (MIRT003827)
- hsa-mir-107 (MIRT004756)
- hsa-mir-128-3p (MIRT004757)
- hsa-mir-223-3p (MIRT006237)
- hsa-mir-27a-3p (MIRT006357)
- hsa-mir-590-3p (MIRT016198)
- hsa-mir-103a-3p (MIRT027232)
- hsa-mir-101-3p (MIRT027385)
- hsa-mir-16-5p (MIRT032006)
- hsa-mir-503-5p (MIRT437907)
- hsa-mir-182-5p (MIRT437908)
- hsa-mir-25-3p (MIRT438342)
- hsa-mir-510-3p (MIRT476879)
- hsa-mir-4639-5p (MIRT476880)
- hsa-mir-2052 (MIRT476881)
- hsa-mir-4328 (MIRT476882)
- hsa-mir-4698 (MIRT476883)
- hsa-mir-570-5p (MIRT476884)
- hsa-mir-548ba (MIRT476885)
- hsa-mir-548ai (MIRT476886)
- hsa-mir-548ag (MIRT476887)
- hsa-mir-203a-3p (MIRT476888)
- hsa-mir-1303 (MIRT548240)
- hsa-mir-664b-3p (MIRT548241)
- hsa-mir-190a-3p (MIRT548242)
- hsa-mir-579-3p (MIRT548243)
- hsa-mir-380-3p (MIRT548244)
- hsa-mir-411-3p (MIRT548245)
- hsa-mir-379-3p (MIRT548246)
- hsa-mir-4495 (MIRT548247)
- hsa-mir-656-3p (MIRT548248)
- hsa-mir-5011-5p (MIRT548249)
- hsa-mir-5694 (MIRT548250)
- hsa-mir-32-5p (MIRT731444)
- hsa-mir-92a-3p (MIRT731599)
- hsa-mir-384 (MIRT732430)
- hsa-mir-363-3p (MIRT733161)
- hsa-mir-182-3p (MIRT733613)
- hsa-mir-155-3p (MIRT733887)
- hsa-mir-770-5p (MIRT734692)
- hsa-mir-155-5p (MIRT734975)
- hsa-mir-24-3p (MIRT735427)
- hsa-mir-223-5p (MIRT735433)
- hsa-mir-367-3p (MIRT735508)
- hsa-mir-1302 (MIRT737261)
- hsa-mir-144-3p (MIRT737623)
- hsa-mir-296-3p (MIRT739516)
- hsa-mir-3122 (MIRT739981)
- hsa-mir-3192-5p (MIRT741013)
- hsa-mir-3529-3p (MIRT741492)
- hsa-mir-3913-5p (MIRT742863)
- hsa-mir-4252 (MIRT743330)
- hsa-mir-4298 (MIRT743811)
- hsa-mir-4425 (MIRT744274)
- hsa-mir-4701-5p (MIRT747306)
- hsa-mir-588 (MIRT751553)
- hsa-mir-6823-5p (MIRT756921)
- hsa-mir-92a-1-5p (MIRT760517)
miRNA Products
Inhibitory RNA Products
- Origene sirna, shrna, and RNAi products in human, mouse, rat for FBXW7
- Browse OriGene Inhibitory RNA Products For FBXW7
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for FBXW7
- genomics-online: shRNA clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
Clone Products
-
OriGene ORF clones in human for FBXW7
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Sino Biological Human cDNA Clone for FBXW7
- VectorBuilder custom plasmid, inducible vectors for FBXW7
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for FBXW7
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for FBXW7
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for FBXW7
- Applied Biological Materials Clones for FBXW7
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
Cell Line Products
No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for FBXW7 Gene
Localization for FBXW7 Gene
Subcellular locations from UniProtKB/Swiss-Prot for FBXW7 Gene
- Isoform 1: Nucleus, nucleoplasm.
- Isoform 2: Cytoplasm.
- Isoform 3: Nucleus, nucleolus.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytosol | 5 |
| plasma membrane | 1 |
| cytoskeleton | 1 |
- Nucleoplasm (3)
- Vesicles (3)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005634 | nucleus | IEA | -- |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005730 | nucleolus | IEA | -- |
| GO:0005737 | cytoplasm | IDA | 23858059 |
| GO:0005829 | cytosol | TAS | -- |
Pathways & Interactions for FBXW7 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Signaling by NOTCH1 | ||
| 2 | Notch signaling pathway (KEGG) |
.30
|
|
| 3 | Cell cycle Role of SCF complex in cell cycle regulation | ||
| 4 | Class I MHC mediated antigen processing and presentation | ||
| 5 | Chaperonin-mediated protein folding |
.94
|
|
Pathways by source for FBXW7 Gene
1 Sino Biological pathway for FBXW7 Gene
4 BioSystems pathways for FBXW7 Gene
21 Reactome pathways for FBXW7 Gene
1 KEGG pathway for FBXW7 Gene
7 GeneGo (Thomson Reuters) pathways for FBXW7 Gene
1 R&D Systems pathway for FBXW7 Gene
2 Qiagen pathways for FBXW7 Gene
Interacting Proteins for FBXW7 Gene
Selected Interacting proteins:
Q969H0-FBXW7_HUMAN
ENSP00000281708
for FBXW7 Gene via
IID
MINT
UniProtKB
STRING
GPS-Prot Interaction Network for FBXW7
SIGNOR curated interactions for FBXW7 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0001944 | vasculature development | TAS | 21123947 |
| GO:0007062 | sister chromatid cohesion | IMP | 15917200 |
| GO:0010868 | negative regulation of triglyceride biosynthetic process | ISS | -- |
| GO:0010883 | regulation of lipid storage | ISS | -- |
Transcripts for FBXW7 Gene
mRNA/cDNA for FBXW7 Gene
- (16) REFSEQ mRNAs :
- (26) Additional mRNA sequences :
- (139) Selected AceView cDNA sequences:
- (17) Ensembl transcripts including schematic representations, and UCSC links where relevant :
-
- ENST00000263981 3743 bps (uc003imq.4)
- ENST00000281708 4896 bps (uc003imt.4)
- ENST00000296555 3587 bps (uc003imr.4)
- ENST00000393956 3540 bps (uc011cih.3)
- ENST00000603548 5653 bps (uc011cii.3)
Unigene Clusters for FBXW7 Gene
- F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase:
- Representative Sequences:
CRISPR Products
-
OriGene CRISPR knockouts for FBXW7
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for FBXW7
- genomics-online: gRNA clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- Applied Biological Materials CRISPR for FBXW7
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for FBXW7
- GenScript: Design CRISPR guide RNA sequences for FBXW7
miRNA Products
Inhibitory RNA Products
- Origene sirna, shrna, and RNAi products in human, mouse, rat for FBXW7
- Browse OriGene Inhibitory RNA Products For FBXW7
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for FBXW7
- genomics-online: shRNA clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
Clone Products
-
OriGene ORF clones in human for FBXW7
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Sino Biological Human cDNA Clone for FBXW7
- VectorBuilder custom plasmid, inducible vectors for FBXW7
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for FBXW7
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for FBXW7
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for FBXW7
- Applied Biological Materials Clones for FBXW7
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | ^ | 4 | ^ | 5a | · | 5b | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10a | · | 10b | ^ | 11 | ^ | 12 | ^ | 13 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | - | |||||||||||||||||||||||||||
| SP2: | - | - | - | - | - | ||||||||||||||||||||||||||
| SP3: | - | - | |||||||||||||||||||||||||||||
| SP4: | |||||||||||||||||||||||||||||||
| SP5: |
Expression for FBXW7 Gene
mRNA differential expression in normal tissues according to GTEx for FBXW7 Gene
This gene is overexpressed in Brain - Frontal Cortex (BA9) (x4.6).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for FBXW7 Gene
NURSA nuclear receptor signaling pathways regulating expression of FBXW7 Gene:
FBXW7SOURCE GeneReport for Unigene cluster for FBXW7 Gene:
Hs.561245mRNA Expression by UniProt/SwissProt for FBXW7 Gene:
Q969H0-FBXW7_HUMAN
Tissue specificity:
Isoform 1 is widely expressed. Isoform 3 is expressed in brain.
Evidence on tissue expression from TISSUES for FBXW7 Gene
- Nervous system(4.9)
- Eye(2.8)
- Intestine(2.5)
- Lung(2.3)
- Skin(2.2)
- Lymph node(2)
Primer Products
-
OriGene qPCR primer pairs for FBXW7
- genomics-online: primer clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
Gene Expression Assay Products
No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for FBXW7 Gene
Orthologs for FBXW7 Gene
This gene was present in the common ancestor of animals and fungi.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| chimpanzee (Pan troglodytes) |
Mammalia | FBXW7 34 33 |
|
OneToOne | |
| dog (Canis familiaris) |
Mammalia | FBXW7 34 33 |
|
OneToOne | |
| cow (Bos Taurus) |
Mammalia | FBXW7 34 33 |
|
OneToOne | |
| rat (Rattus norvegicus) |
Mammalia | Fbxw7 33 |
|
||
| mouse (Mus musculus) |
Mammalia | Fbxw7 16 34 33 |
|
||
| platypus (Ornithorhynchus anatinus) |
Mammalia | FBXW7 34 |
|
OneToOne | |
| oppossum (Monodelphis domestica) |
Mammalia | FBXW7 34 |
|
OneToOne | |
| chicken (Gallus gallus) |
Aves | FBXW7 34 33 |
|
OneToOne | |
| lizard (Anolis carolinensis) |
Reptilia | FBXW7 34 |
|
OneToOne | |
| tropical clawed frog (Silurana tropicalis) |
Amphibia | fbxw7 33 |
|
||
| Str.6549 33 |
|
||||
| African clawed frog (Xenopus laevis) |
Amphibia | Xl.32837 33 |
|
||
| zebrafish (Danio rerio) |
Actinopterygii | fbxw7 33 |
|
||
| FBXW7 34 |
|
OneToOne | |||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP005359 33 |
|
||
| fruit fly (Drosophila melanogaster) |
Insecta | ago 34 35 |
|
OneToOne | |
| worm (Caenorhabditis elegans) |
Secernentea | sel-10 34 |
|
OneToOne | |
| baker's yeast (Saccharomyces cerevisiae) |
Saccharomycetes | MET30 34 |
|
OneToMany | |
| CDC4 36 |
|
|
|||
| sea squirt (Ciona savignyi) |
Ascidiacea | CSA.3633 34 |
|
OneToOne |
- Species where no ortholog for FBXW7 was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Ashbya gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- Alicante grape (Vitis vinifera)
- alpha proteobacteria (Wolbachia pipientis)
- amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- barley (Hordeum vulgare)
- beta proteobacteria (Neisseria meningitidis)
- bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- common water flea (Daphnia pulex)
- corn (Zea mays)
- E. coli (Escherichia coli)
- filamentous fungi (Aspergillus nidulans)
- Firmicute bacteria (Streptococcus pneumoniae)
- fission yeast (Schizosaccharomyces pombe)
- green algae (Chlamydomonas reinhardtii)
- honey bee (Apis mellifera)
- K. lactis yeast (Kluyveromyces lactis)
- loblloly pine (Pinus taeda)
- malaria parasite (Plasmodium falciparum)
- medicago trunc (Medicago Truncatula)
- moss (Physcomitrella patens)
- orangutan (Pongo pygmaeus)
- pig (Sus scrofa)
- rainbow trout (Oncorhynchus mykiss)
- rice (Oryza sativa)
- rice blast fungus (Magnaporthe grisea)
- schistosome parasite (Schistosoma mansoni)
- sea anemone (Nematostella vectensis)
- sea urchin (Strongylocentrotus purpuratus)
- sorghum (Sorghum bicolor)
- soybean (Glycine max)
- stem rust fungus (Puccinia graminis)
- sugarcane (Saccharum officinarum)
- thale cress (Arabidopsis thaliana)
- tomato (Lycopersicon esculentum)
- toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- wheat (Triticum aestivum)
Evolution for FBXW7 Gene
- ENSEMBL:
- Gene Tree for FBXW7 (if available)
- TreeFam:
- Gene Tree for FBXW7 (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for FBXW7: view image
Paralogs for FBXW7 Gene
(19) SIMAP similar genes for FBXW7 Gene using alignment to 8 proteins:
- FBXW7_HUMAN
- A7BJS8_HUMAN
- B7Z2C8_HUMAN
- G0Z2K0_HUMAN
- H9CWI2_HUMAN
- H9CWI3_HUMAN
- S4R3N3_HUMAN
- S4R3U4_HUMAN
Variants for FBXW7 Gene
| SNP ID | Clin | Chr 04 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| rs1057519895 | likely-pathogenic, Adenocarcinoma of stomach, Squamous cell carcinoma of lung, Adenoid cystic carcinoma, Uterine Carcinosarcoma, Malignant neoplasm of body of uterus, Carcinoma of esophagus, Squamous cell carcinoma of the head and neck, Medulloblastoma, Chronic lymphocytic leukemia, Neoplasm of the breast, Colorectal Neoplasms, Ovarian Serous Cystadenocarcinoma, Adenocarcinoma of lung, Uterine cervical neoplasms | 152,328,232(-) | C/A/G/T | coding_sequence_variant, genic_downstream_transcript_variant, missense_variant | |
| rs1057519896 | likely-pathogenic, Carcinoma of esophagus, Squamous cell carcinoma of the head and neck, Adenocarcinoma of stomach, Uterine cervical neoplasms, Ovarian Serous Cystadenocarcinoma, Uterine Carcinosarcoma, Squamous cell carcinoma of lung, Transitional cell carcinoma of the bladder, Malignant melanoma of skin, Colorectal Neoplasms, Malignant neoplasm of body of uterus, Chronic lymphocytic leukemia | 152,326,136(-) | C/A/T | coding_sequence_variant, genic_downstream_transcript_variant, missense_variant | |
| rs149680468 | likely-pathogenic, Malignant neoplasm of body of uterus, Ovarian Serous Cystadenocarcinoma, Squamous cell carcinoma of lung, Transitional cell carcinoma of the bladder, Uterine Carcinosarcoma, Squamous cell carcinoma of the head and neck, Adenocarcinoma of stomach, Carcinoma of esophagus, Colorectal Neoplasms, Malignant melanoma of skin, Chronic lymphocytic leukemia, Uterine cervical neoplasms | 152,326,137(-) | G/A/C/T | coding_sequence_variant, genic_downstream_transcript_variant, missense_variant | |
| rs747241612 | likely-pathogenic, Malignant neoplasm of body of uterus, Uterine Carcinosarcoma, Squamous cell carcinoma of lung, Glioblastoma, Squamous cell carcinoma of the head and neck, Transitional cell carcinoma of the bladder, Adenocarcinoma of stomach, Malignant melanoma of skin, Colorectal Neoplasms, Neoplasm of the breast, Papillary renal cell carcinoma, sporadic, Uterine cervical neoplasms | 152,326,215(-) | G/C | coding_sequence_variant, genic_downstream_transcript_variant, missense_variant | |
| rs759610249 | likely-pathogenic, Malignant melanoma of skin, Malignant neoplasm of body of uterus, Glioblastoma, Transitional cell carcinoma of the bladder, Uterine Carcinosarcoma, Squamous cell carcinoma of the head and neck, Colorectal Neoplasms, Uterine cervical neoplasms | 152,323,032(-) | C/T | coding_sequence_variant, genic_downstream_transcript_variant, missense_variant |
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| nsv830116 | CNV | gain | 17160897 |
| nsv523798 | CNV | loss | 19592680 |
| nsv516218 | CNV | loss | 19592680 |
| nsv4553 | CNV | insertion | 18451855 |
| nsv1131833 | CNV | deletion | 24896259 |
| nsv1031970 | CNV | gain | 25217958 |
| dgv5442n100 | CNV | loss | 25217958 |
| dgv5441n100 | CNV | loss | 25217958 |
| dgv5440n100 | CNV | loss | 25217958 |
| dgv5439n100 | CNV | loss | 25217958 |
Residual Variation Intolerance Score:
36.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
2.36;
41.88% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for FBXW7 Gene
SNP Genotyping and Copy Number Assay Products
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for FBXW7 Gene
Disorders for FBXW7 Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| hyperlucent lung |
|
|
| lung adenoid cystic carcinoma |
|
|
| serous cystadenocarcinoma |
|
|
| bladder adenocarcinoma |
|
|
| cystadenocarcinoma |
|
|
Search FBXW7 in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for FBXW7
- Genetic Association Database
- (GAD)
- Human Genome Epidemiology Navigator
- (HuGE)
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
No data available for UniProtKB/Swiss-Prot and Genatlas for FBXW7 Gene
Publications for FBXW7 Gene
- The SV40 large T antigen contains a decoy phosphodegron that mediates its interactions with Fbw7/hCdc4. (PMID: 15611062) Welcker M … Clurman BE (The Journal of biological chemistry 2005) 3 4 22 58
- SEL-10 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. (PMID: 12354302) Li J … Gurney ME (Journal of neurochemistry 2002) 3 4 22 58
- A family of mammalian F-box proteins. (PMID: 10531037) Winston JT … Harper JW (Current biology : CB 1999) 2 3 4 58
- NOTCH2 Hajdu-Cheney Mutations Escape SCFFBW7-Dependent Proteolysis to Promote Osteoporosis. (PMID: 29149593) Fukushima H … Inuzuka H (Molecular cell 2017) 3 4 58
- Structural basis of N-Myc binding by Aurora-A and its destabilization by kinase inhibitors. (PMID: 27837025) Richards MW … Bayliss R (Proceedings of the National Academy of Sciences of the United States of America 2016) 3 4 58
Products for FBXW7 Gene
- R&D Systems Antibodies for FBXW7 (FBXW7/Cdc4)
- Browse R&D Systems for Human Recombinant Proteins
- R&D Systems Proteome Profiler Antibody Arrays and other biochemical assays for FBXW7 (FBXW7/Cdc4)
- Browse Human Proteins and Enzymes
- Browse Primary Antibodies
- Browse Proteins and Enzymes
- Browse ELISAs
- Browse Activity Assays
- Browse cDNA Clones
- Browse Cell Culture Products
- Browse Cell Selection and Detection Kits
- Browse DNA Damage and Repair Kits
- Browse ELISpot/FluoroSpot Kits and Development Modules
- Browse Flow Cytometry Kits
- Browse Immunoprecipitation Assays
- Browse Luminex Assays
- Browse Peptides
- Browse Proteome Profiler Antibody Arrays
- Browse Small Molecules
- Custom Antibody ServicesOriGene Antibodies for FBXW7
- Browse OriGene ELISA Kits
- Custom Assay Services
- OriGene Purified Proteins for FBXW7
- Search Origene for MassSpec and Protein Over-expression Lysates for FBXW7
- Origene Custom Protein Services for FBXW7
- Origene sirna, shrna, and RNAi products in human, mouse, rat for FBXW7
- Browse OriGene Inhibitory RNA Products For FBXW7
- OriGene qPCR primer pairs for FBXW7
- OriGene CRISPR knockouts for FBXW7
- OriGene ORF clones in human for FBXW7
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For FBXW7
- GenScript: Next-day shipping of latest version cDNA ORF clones for FBXW7 in any vector
- GenScript Custom Purified and Recombinant Proteins Services for FBXW7
- GenScript Custom Assay Services for FBXW7
- GenScript Custom overexpressing Cell Line Services for FBXW7
- GenScript: Design CRISPR guide RNA sequences for FBXW7
- Design optimal peptide antigens
- CloneReady with Over 120,000 Genes
- Gene Synthesis: Any Gene in Any Vector
- Vector-based siRNA and miRNA, Ready for Transfection
- Gene Mutant Library, Variants up to 10^11
- Plasmid Preparation
- GenScript Custom Peptide Services for FBXW7
- Sino Biological Human cDNA Clone for FBXW7
- Browse Sino Biological Recombinant Proteins
- Browse Sino Biological Cell Lysates
- Browse Sino Biological Antibodies
- Browse Sino Biological Assays
- Browse cDNA Clones
- Browse ELISA Kits
- Browse CRO Services
- High-throughput Antibody Production Service
- Protein Production Service
- Epitope Tag Antibodies & Reporter Protein Antibodies
- Lentiviral ORF Clones
- Novus Biologicals Antibodies for FBXW7
- Novus Biologicals proteins and lysates for FBXW7
- Novus Biologicals
- Novus Biologicals Tissue Microarrays
- Browse Antibodies
- Browse Peptides and Proteins
- Browse Lysates
- Browse ELISA Kits
- VectorBuilder custom plasmid, inducible vectors for FBXW7
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for FBXW7
- VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for FBXW7
- antibodies-online: Search results for 138 available FBXW7 Antibodies ranked by validation data
- Compare Top FBXW7 Antibodies
- Recommended
- 12 IF (p) Antibodies (anti-rat)
- 11 WB Antibodies (anti-rat)
- 1 IP Antibody (anti-rat)
- 100 WB Antibodies (anti-human)
- 12 IF (p) Antibodies (anti-human)
- 6 IHC (p) Antibodies (anti-human)
- 5 IP Antibodies (anti-human)
- 2 FACS Antibodies (anti-human)
- 1 IHC Antibody (anti-human)
- 30 WB Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 2 FACS Antibodies (anti-mouse)
- 1 IHC Antibody (anti-mouse)
- 1 IP Antibody (anti-mouse)
- antibodies-online: Search results for 12 available FBXW7 Elisa Kits ranked by validation data
- Compare Top FBXW7 Elisa Kits
- antibodies-online: Search results for 4 available FBXW7 Proteins ranked by validation data
- Compare Top FBXW7 Proteins
- GeneTex FBXW7 Antibody for FBXW7
- Search GeneTex for Proteins for FBXW7
- Santa Cruz Biotechnology (SCBT) Antibodies for FBXW7
- Search Santa Cruz Biotechnology (SCBT) for FBXW7 siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for FBXW7
- genomics-online: cdna clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- orf clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- genomics-online: gRNA clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- genomics-online: primer clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- genomics-online: shRNA clones - Search results for 122 available FBXW7 gene related products
- Overview of 122 available FBXW7 gene related products
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for FBXW7
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for FBXW7
- GeneCopoeia miRNA 3'UTR Target clone products for FBXW7
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for FBXW7
Sources for FBXW7 Gene
- (1) GeneCards
- (2) HGNC
- (3) EntrezGene
- (4) Swiss-Prot
- (5) Ensembl
- (6) OMIM
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) GO
- (12) TrEMBL
- (13) InterPro
- (14) ProtoNet
- (15) Blocks
- (16) MGI
- (17) IUBMB
- (18) KEGG
- (19) MINT
- (20) STRING
- (21) IntAct
- (22) Novoseek
- (23) PharmGKB
- (24) DrugBank
- (25) HMDB
- (26) UniGene
- (27) AceView
- (28) ASD
- (29) ECgene
- (30) GeneAnnot
- (31) CGAP SAGE
- (32) SOURCE
- (33) HomoloGene
- (34) PanEnsembl
- (35) euGenes
- (36) SGD
- (37) FlyBase
- (38) WormBase
- (39) Pseudogene
- (40) DGV
- (41) dbSNP
- (42) GenAtlas
- (43) HGMD
- (44) GAD
- (45) BGMUT
- (46) HuGE
- (47) Atlas
- (48) Cell Signaling Technology
- (49) GenBank
- (50) H-invDB
- (51) HORDE
- (52) HUGE
- (53) IMGT
- (54) Leiden
- (55) miRBase
- (56) DME
- (57) OriGene
- (58) PubMed
- (59) R&D Systems
- (60) TGDB
- (61) Tocris
- (62) Abcam
- (63) Novus Biologicals
- (64) ProSpec
- (65) Sino Biological
- (66) GenScript
- (67) Qiagen
- (68) Cloud-Clone Corp.
- (69) OCA
- (70) Proteopedia
- (71) MOPED
- (72) neXtProt
- (73) Reactome
- (74) GeneGo (Thomson Reuters)
- (75) fRNAdb
- (76) DISEASES
- (77) SIMAP
- (78) GenomeRNAi
- (79) LifeMap
- (80) miRTarBase
- (81) MalaCards
- (82) Invitrogen
- (83) BitterDB
- (84) Vector BioLabs
- (85) ESI-BIO
- (86) RefSeq
- (87) BioSystems
- (88) MaxQB
- (89) IUPHAR
- (90) BioGPS
- (91) Illumina
- (92) COMPARTMENTS
- (93) HOMER
- (94) PaxDb
- (95) ApexBio
- (96) Addgene
- (97) antibodies-online
- (98) CYP
- (99) NONCODE
- (100) SwitchGear Genomics
- (101) TreeFam
- (102) PathCards
- (103) GeneReviews
- (104) GeneTex
- (105) Taconic Biosciences
- (106) GTEx
- (107) ProteomicsDB
- (108) SCBT
- (109) DGIdb
- (110) ClinicalTrials
- (111) FDA Approved Drugs
- (112) RVIS
- (113) SIGNOR
- (114) diseasecard
- (115) NIH Rare Diseases
- (116) Orphanet
- (117) UMLS
- (118) GTR
- (119) Disease Ontology
- (120) Genetics Home Reference
- (121) MeSH
- (122) MedlinePlus
- (123) CDC
- (124) NINDS
- (125) NCBI Bookshelf
- (126) ClinVar
- (127) Gene Damage Index
- (128) ViGene Biosciences
- (129) HPO
- (130) UDN
- (131) VISTA
- (132) FANTOM5
- (133) ENCODE
- (134) ProSci
- (135) Horizon
- (136) NURSA
- (137) IID
- (138) Cyagen
- (139) VectorBuilder
- (140) SNPedia
- (141) BRCA Exchange
- (142) St John's Lab
- (143) CIViC
- (144) ProteoGenix
- (145) dbSUPER
- (146) TISSUES
- (147) Gene ORGANizer
- (148) abm
- (149) CrownBio
- (150) Human Protein Atlas
- (151) GWAS Catalog
- (152) Monarch Initiative
- (153) DataMed
- (154) HumanCyc
- (155) genomics-online
- (156) UCNEbase
- (157) EPDnew
- (158) Applied Biosystems
- (159) GlyConnect
- (160) Aminode
- (161) GeneCopoeia
- (162) GPS-Prot
- (163) RNACentral
- (164) LncRNADisease
- (165) LOVD
- (166) miR2Disease
- (167) DiseaseEnhancer
- (168) Boster Bio
- (169) Biorbyt