Aliases for EPHX3 Gene

Aliases for EPHX3 Gene

  • Epoxide Hydrolase 3 2 3 4 5
  • Abhydrolase Domain-Containing Protein 9 3 4
  • Abhydrolase Domain Containing 9 2 3
  • ABHD9 3 4
  • EH3 3 4
  • EC 4
  • FLJ22408 2
  • EPHX3 5

External Ids for EPHX3 Gene

Previous HGNC Symbols for EPHX3 Gene

  • ABHD9

Previous GeneCards Identifiers for EPHX3 Gene

  • GC19M015199
  • GC19M015337
  • GC19M014905

Summaries for EPHX3 Gene

GeneCards Summary for EPHX3 Gene

EPHX3 (Epoxide Hydrolase 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include hydrolase activity. An important paralog of this gene is EPHX4.

UniProtKB/Swiss-Prot Summary for EPHX3 Gene

  • Catalyzes the hydrolysis of epoxide-containing fatty acids. Active in vitro against epoxyeicosatrienoic acids (EETs) including 8,9-EET, 9,10-EET, 11,12-EET and 14,15-EET and leukotoxin.

Additional gene information for EPHX3 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for EPHX3 Gene

Genomics for EPHX3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for EPHX3 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J015231 Promoter/Enhancer 1.6 EPDnew Ensembl ENCODE CraniofacialAtlas 600.7 +0.9 852 2.8 CTCF KDM1A POLR2A EZH2 PKNOX1 SCRT2 DPF2 ZBTB48 YY1 IKZF1 EPHX3 ENSG00000269635 NOTCH3 lnc-OR7C2-1
GH19J015194 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 12.9 +35.3 35253 7.2 CREB1 ZBTB33 RFX5 TARDBP JUND ZSCAN4 PATZ1 ZNF600 ZNF341 SCRT2 NOTCH3 EPHX3 RNU6-782P AKAP8 OR7C1 RF00017-2858 ILVBL lnc-OR7C2-1
GH19J015295 Promoter/Enhancer 1.5 Ensembl ENCODE dbSUPER 11.6 -65.3 -65257 5.7 CREB1 GATAD2A ATF7 PRDM10 ZNF629 TFE3 SOX13 IKZF1 CTCF ZNF692 AKAP8 ZNF333 BRD4 EPHX3 RASAL3 ENSG00000268189 RNU6-782P CYP4F24P lnc-EPHX3-7 lnc-CYP4F22-6
GH19J015279 Promoter/Enhancer 1.4 Ensembl ENCODE dbSUPER 11.3 -48.9 -48907 6.1 CEBPG GATAD2A ZBTB33 PRDM10 IKZF1 ZNF692 JUND PRDM1 ZIC2 PKNOX1 AKAP8 DDX39A BRD4 EPHX3 AKAP8L RNU6-782P lnc-EPHX3-5 lnc-EPHX3-7 lnc-OR7C2-1
GH19J015316 Promoter/Enhancer 1.3 Ensembl ENCODE dbSUPER 11.3 -85.8 -85836 6.2 ZNF629 SOX13 IKZF1 POLR2A JUND PRDM1 IKZF2 HDAC1 ZNF600 SCRT2 AKAP8 DDX39A BRD4 NOTCH3 EPHX3 AKAP8L RAB8A lnc-CYP4F22-6 lnc-OR7C2-1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around EPHX3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for EPHX3

Top Transcription factor binding sites by QIAGEN in the EPHX3 gene promoter:
  • Egr-2
  • NF-1
  • Nkx2-5
  • POU2F1
  • POU2F1c
  • RP58

Genomic Locations for EPHX3 Gene

Genomic Locations for EPHX3 Gene
6,535 bases
Minus strand
6,517 bases
Minus strand

Genomic View for EPHX3 Gene

Genes around EPHX3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
EPHX3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for EPHX3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EPHX3 Gene

Proteins for EPHX3 Gene

  • Protein details for EPHX3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Epoxide hydrolase 3
    Protein Accession:
    Secondary Accessions:
    • A3KMR3

    Protein attributes for EPHX3 Gene

    360 amino acids
    Molecular mass:
    40909 Da
    Quaternary structure:
    No Data Available

neXtProt entry for EPHX3 Gene

Post-translational modifications for EPHX3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for EPHX3 Gene

No data available for DME Specific Peptides for EPHX3 Gene

Domains & Families for EPHX3 Gene

Gene Families for EPHX3 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for EPHX3 Gene

  • Alpha/beta hydrolase fold
  • Alpha/beta hydrolase fold signature
  • Epoxide hydrolase signature

Suggested Antigen Peptide Sequences for EPHX3 Gene

GenScript: Design optimal peptide antigens:
  • Abhydrolase domain-containing protein 9 (EPHX3_HUMAN)

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
  • Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
genes like me logo Genes that share domains with EPHX3: view

Function for EPHX3 Gene

Molecular function for EPHX3 Gene

UniProtKB/Swiss-Prot Function:
Catalyzes the hydrolysis of epoxide-containing fatty acids. Active in vitro against epoxyeicosatrienoic acids (EETs) including 8,9-EET, 9,10-EET, 11,12-EET and 14,15-EET and leukotoxin.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=an epoxide + H2O = an ethanediol; Xref=Rhea:RHEA:19037, ChEBI:CHEBI:15377, ChEBI:CHEBI:32955, ChEBI:CHEBI:140594; EC=; Evidence={ECO:0000269|PubMed:22798687};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=9,10-epoxyoctadecanoate + H2O = 9,10-dihydroxyoctadecanoate; Xref=Rhea:RHEA:45352, ChEBI:CHEBI:15377, ChEBI:CHEBI:85195, ChEBI:CHEBI:85197; Evidence={ECO:0000269|PubMed:22798687};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=9,10-epoxy-(12Z)-octadecenoate + H2O = 9,10-dihydroxy-(12Z)-octadecenoate; Xref=Rhea:RHEA:44032, ChEBI:CHEBI:15377, ChEBI:CHEBI:84023, ChEBI:CHEBI:84027; Evidence={ECO:0000269|PubMed:22798687};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoate + H2O = 8,9-dihydroxy-(5Z,11Z,14Z)-eicosatrienoate; Xref=Rhea:RHEA:44048, ChEBI:CHEBI:15377, ChEBI:CHEBI:84025, ChEBI:CHEBI:84032; Evidence={ECO:0000269|PubMed:22798687};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=11,12-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H2O = 11,12-dihydroxy-(5Z,8Z,14Z)-eicosatrienoate; Xref=Rhea:RHEA:44044, ChEBI:CHEBI:15377, ChEBI:CHEBI:76625, ChEBI:CHEBI:84031; Evidence={ECO:0000269|PubMed:22798687};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=14,15-epoxy-(5Z,8Z,11Z)-eicosatrienoate + H2O = 14,15-dihydroxy-(5Z,8Z,11Z)-eicosatrienoate; Xref=Rhea:RHEA:44040, ChEBI:CHEBI:15377, ChEBI:CHEBI:84024, ChEBI:CHEBI:84029; Evidence={ECO:0000269|PubMed:22798687};.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=30 uM for 8,9-EET {ECO:0000269|PubMed:22798687}; KM=80 uM for 11,12-EET {ECO:0000269|PubMed:22798687}; KM=130 uM for 14,15-EET {ECO:0000269|PubMed:22798687}; KM=25 uM for leukotoxin {ECO:0000269|PubMed:22798687}; Vmax=12 umol/min/mg enzyme with 8,9-EET as substrate {ECO:0000269|PubMed:22798687}; Vmax=50 umol/min/mg enzyme with 11,12-EET as substrate {ECO:0000269|PubMed:22798687}; Vmax=60 umol/min/mg enzyme with 14,15-EET as substrate {ECO:0000269|PubMed:22798687}; Vmax=22 umol/min/mg enzyme with leukotoxin as substrate {ECO:0000269|PubMed:22798687};
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by 1-(1-acetylpiperidin-4-yl)-3-(4-(trifl uoromethoxy)phenyl)urea (TPAU), 1-cyclohexyl-3-dodecylurea (CDU), 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA), 1-((3S, 5S, 7S)-adamantan-1-yl)-3-(5-(2-(2-ethoxyethoxy) ethoxy)pentyl)urea (AEPU) and to a lesser extent by 8-(3-((3S, 5S, 7S)-adamantan-1-yl)ureido) octanoic acid (AUOA).

Enzyme Numbers (IUBMB) for EPHX3 Gene

Phenotypes From GWAS Catalog for EPHX3 Gene

Gene Ontology (GO) - Molecular Function for EPHX3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003824 catalytic activity IEA --
GO:0004301 epoxide hydrolase activity IMP 22798687
GO:0016787 hydrolase activity IEA,IBA 21873635
genes like me logo Genes that share ontologies with EPHX3: view
genes like me logo Genes that share phenotypes with EPHX3: view

Animal Models for EPHX3 Gene

MGI Knock Outs for EPHX3:

Animal Model Products

CRISPR Products

miRNA for EPHX3 Gene

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for EPHX3

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for EPHX3 Gene

Localization for EPHX3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for EPHX3 Gene

Microsome membrane. Single-pass membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for EPHX3 gene
Compartment Confidence
endoplasmic reticulum 3
plasma membrane 2
mitochondrion 2
extracellular 1
cytoskeleton 1
peroxisome 1
nucleus 1
cytosol 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for EPHX3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IEA --
GO:0016020 membrane IEA,IDA 22798687
GO:0016021 integral component of membrane IEA --
GO:0031090 organelle membrane IEA --
GO:0043231 intracellular membrane-bounded organelle IEA,IDA 22798687
genes like me logo Genes that share ontologies with EPHX3: view

Pathways & Interactions for EPHX3 Gene

PathCards logo

SuperPathways for EPHX3 Gene

No Data Available

Interacting Proteins for EPHX3 Gene

Gene Ontology (GO) - Biological Process for EPHX3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006629 lipid metabolic process IEA --
GO:0097176 epoxide metabolic process IDA 22798687
genes like me logo Genes that share ontologies with EPHX3: view

No data available for Pathways by source and SIGNOR curated interactions for EPHX3 Gene

Drugs & Compounds for EPHX3 Gene

No Compound Related Data Available

Transcripts for EPHX3 Gene

mRNA/cDNA for EPHX3 Gene

6 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for EPHX3

Alternative Splicing Database (ASD) splice patterns (SP) for EPHX3 Gene

No ASD Table

Relevant External Links for EPHX3 Gene

GeneLoc Exon Structure for

Expression for EPHX3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for EPHX3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for EPHX3 Gene

This gene is overexpressed in Skin - Sun Exposed (Lower leg) (x13.1), Esophagus - Mucosa (x12.6), Skin - Not Sun Exposed (Suprapubic) (x11.7), and Vagina (x5.4).

Protein differential expression in normal tissues from HIPED for EPHX3 Gene

This gene is overexpressed in Liver, secretome (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for EPHX3 Gene

Protein tissue co-expression partners for EPHX3 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for EPHX3

SOURCE GeneReport for Unigene cluster for EPHX3 Gene:


Evidence on tissue expression from TISSUES for EPHX3 Gene

  • Nervous system(4.4)
  • Skin(2.5)
  • Kidney(2)
genes like me logo Genes that share expression patterns with EPHX3: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for EPHX3 Gene

Orthologs for EPHX3 Gene

This gene was present in the common ancestor of animals.

Orthologs for EPHX3 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia EPHX3 30 31
  • 99.44 (n)
(Bos Taurus)
Mammalia EPHX3 30 31
  • 86.3 (n)
(Canis familiaris)
Mammalia EPHX3 31
  • 82 (a)
(Mus musculus)
Mammalia Ephx3 30 17 31
  • 80.78 (n)
(Rattus norvegicus)
Mammalia Ephx3 30
  • 80.07 (n)
(Monodelphis domestica)
Mammalia EPHX3 31
  • 63 (a)
(Ornithorhynchus anatinus)
Mammalia EPHX3 31
  • 61 (a)
(Anolis carolinensis)
Reptilia EPHX3 31
  • 43 (a)
(Danio rerio)
Actinopterygii AL954361.1 31
  • 40 (a)
(Caenorhabditis elegans)
Secernentea ceeh-1 31
  • 29 (a)
ceeh-2 31
  • 27 (a)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 29 (a)
Species where no ortholog for EPHX3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)

Evolution for EPHX3 Gene

Gene Tree for EPHX3 (if available)
Gene Tree for EPHX3 (if available)
Evolutionary constrained regions (ECRs) for EPHX3: view image

Paralogs for EPHX3 Gene

(1) SIMAP similar genes for EPHX3 Gene using alignment to 2 proteins:

  • M0QX48_HUMAN
genes like me logo Genes that share paralogs with EPHX3: view

Variants for EPHX3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for EPHX3 Gene

SNP ID Clinical significance and condition Chr 19 pos Variation AA Info Type
744463 Benign: not provided 15,231,811(-) G/C SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for EPHX3 Gene

Structural Variations from Database of Genomic Variants (DGV) for EPHX3 Gene

Variant ID Type Subtype PubMed ID
dgv3442n100 CNV loss 25217958
esv34154 CNV loss 18971310
nsv578717 CNV loss 21841781
nsv953983 CNV deletion 24416366

Variation tolerance for EPHX3 Gene

Residual Variation Intolerance Score: 83.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.18; 23.78% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for EPHX3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for EPHX3 Gene

Disorders for EPHX3 Gene

Additional Disease Information for EPHX3

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform

No disorders were found for EPHX3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for EPHX3 Gene

Publications for EPHX3 Gene

  1. EH3 (ABHD9): the first member of a new epoxide hydrolase family with high activity for fatty acid epoxides. (PMID: 22798687) Decker M … Arand M (Journal of lipid research 2012) 3 4
  2. Oxidative damage to biological macromolecules in Prague bus drivers and garagemen: impact of air pollution and genetic polymorphisms. (PMID: 20723587) Bagryantseva Y … Sram RJ (Toxicology letters 2010) 3 41
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. Validation study of genes with hypermethylated promoter regions associated with prostate cancer recurrence. (PMID: 24718283) Stott-Miller M … Stanford JL (Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2014) 3

Products for EPHX3 Gene

Sources for EPHX3 Gene